LOCUS       NZ_CP069508            11469 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter pittii strain FDAARGOS_1215 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP069508
VERSION     NZ_CP069508.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357357
            Assembly: GCF_016889345.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11469)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1649.01x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:49:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,670
            CDSs (total)                      :: 3,575
            Genes (coding)                    :: 3,515
            CDSs (with protein)               :: 3,515
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 23 of 60
            Pseudo Genes (internal stop)      :: 18 of 60
            Pseudo Genes (multiple problems)  :: 11 of 60
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069508.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1215"
                     /culture_collection="DSM:9307"
                     /db_xref="taxon:48296"
                     /plasmid="unnamed4"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            complement(join(11382..11469,1..908))
                     /locus_tag="I6J45_RS18195"
                     /old_locus_tag="I6J45_18195"
     CDS             complement(join(11382..11469,1..908))
                     /locus_tag="I6J45_RS18195"
                     /old_locus_tag="I6J45_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032049752.1"
                     /translation="MIHYSPMSRYTAQKIVDKVGHGAYFYSHFSVEGEDNLFFPKIDKL
                     IKKLTDKYHLDLTSRQRSYRLNTKKEPIADLIVQKRVNSTIFDFWLLITTPNTHKFNTQ
                     LSQINLKPRLSGQRVAEAENVVWNRENEQQEISVIQDYFRDQEKFKFVLQKPYLKLNFG
                     NGKYVELVRLSHSTKNSKKYASNRKKSEKNYTWTWRYDEPTVHLIEKKYKEIINDLISN
                     PNKSVGIGKWQQLNADLQHYTVFKGNRHQVGRLFTQAVGYHYKKGQSNLRNAEYYQPLT
                     LSYLPRQENYAEDFIQFVILRRLFEETGREFGKENVHEENYNQLINQYLI"
                     /name="hypothetical protein"
     gene            3243..4172
                     /gene="repM"
                     /locus_tag="I6J45_RS18200"
                     /old_locus_tag="I6J45_18200"
     CDS             3243..4172
                     /gene="repM"
                     /locus_tag="I6J45_RS18200"
                     /old_locus_tag="I6J45_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017401951.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005237331.1"
                     /translation="MSKELVVKTNRLNQAFQTLSLSEFHIVQLAIVDARHTGTGLSTDT
                     PLRIDALRYAEVFGTTRQNAYQRMKEAEDSLFNRRFSFFDEDGKLVKSRWIQQVKYLDD
                     EGAIELVFTLAVVQGISKIDGIKDFFTQYLLSQTAQLNSTYSARLYELLIQWRAIGKTP
                     VFELATFREQLGIGVNEYKRMDHFKTRVLDLAISEISEKTDIEATYQQHKKGRSISGFS
                     FSFKQKKSKTKSLENQTISGNLDLFSKKMTDSQRHLFSNKLSELPEMSKYSQGTESYPQ
                     FAVRIAEMLKDSEKLKEFAPMLEKVGYR"
                     /name="repM"
     gene            4241..4786
                     /locus_tag="I6J45_RS18205"
                     /old_locus_tag="I6J45_18205"
     CDS             4241..4786
                     /locus_tag="I6J45_RS18205"
                     /old_locus_tag="I6J45_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_171054282.1"
                     /translation="MKALSVIELAQLYGMNRQSIYKRINKGDLSKNSDGKIDFSEALRV
                     FGEPSHNNKDVTTLQSTSVQKETEVDMLKQQVDILKKQLELAHEREVFQREQLEAKDNQ
                     IEAIQRLLEAPKTNMTTFTDQKPSQDIATDPRSEPEPKHDGLTTPEQTENKRIPVPDHV
                     EPEQPKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(4983..>5117)
                     /locus_tag="I6J45_RS18210"
                     /pseudo=""
     CDS             complement(4983..>5117)
                     /locus_tag="I6J45_RS18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /name="cysteine hydrolase"
     gene            5236..5565
                     /locus_tag="I6J45_RS18435"
     CDS             5236..5565
                     /locus_tag="I6J45_RS18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005237346.1"
                     /translation="MKNLQIVIAQALHSYEFEHMEQSISKSISDTILEILVNDGPFEKI
                     DCYLYSKKDSKKILKSSIHYVIAVLERAKYRVQKAQQDNLSPLYICLYVIKKIEGFIEY
                     LNTKF"
                     /name="hypothetical protein"
     gene            5815..6102
                     /locus_tag="I6J45_RS18215"
                     /old_locus_tag="I6J45_18210"
     CDS             5815..6102
                     /locus_tag="I6J45_RS18215"
                     /old_locus_tag="I6J45_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_039890878.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDKYEHG"
                     /name="BrnT family toxin"
     gene            6089..6403
                     /locus_tag="I6J45_RS18220"
                     /old_locus_tag="I6J45_18215"
     CDS             6089..6403
                     /locus_tag="I6J45_RS18220"
                     /old_locus_tag="I6J45_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_057095489.1"
                     /translation="MSMVKYSRKELNEKFSDKQDAEIERLLAKGTVSDDHLDLSDMPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            6626..8764
                     /locus_tag="I6J45_RS18225"
                     /old_locus_tag="I6J45_18220"
     CDS             6626..8764
                     /locus_tag="I6J45_RS18225"
                     /old_locus_tag="I6J45_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="WP_057095490.1"
                     /translation="MIKVIWGTNKEKPIASRQLAETLSSNMSLEGFLYIGYPIVGSPLG
                     PTKFDALLLSESKGIIAFDLVEGTDLTNYINNQDEMASMLEVKLKPYPKLKKGRHLKFD
                     IKTVTFCPAKKNNLPQVEDNLYNIANISNLNEVINSFEWESDEDTFKELKAAIQVVTNI
                     RSGAIRRQTKKENSLGSRLQKLEDSIANLDQHQSEAVIETVDGVQRIRGLAGSGKTIVL
                     ALKVAYLHAQNQSWKIAVTFHTRALKEQFKRLINNFTIEQLGSEPDWDSIDIFNSWGAP
                     GDKENDGMYHRYCVENSIDYYDFQTAKNEFGGDDAFKNVCKLALEQSSSNKIEKYDLIL
                     IDEAQDFPPEFIKLCYKFLKEPKRLVYAYDELQSLNGLSVLPPEQLFGKDSKGVPLVTL
                     EEEDPTKPKKDIILEKCYRNSRPLLATAHTLGFGLTRPKGLIQFFDNDSLWEDVGYSVK
                     KGKIEGGKEVELIRTSESSPLFLEEHTPLEELIKFEVFDSVRDMNQMLFDSIVKNIHED
                     ELKPEDILVINPDPFTTQKNVGIVRKALQQNEIDSEIAGVTTSRDIFRKNGSVTFTGIF
                     RAKGNEAAMVYIIHAQDCADSYDPNHLALIRNRLFTAITRSKAWVRVLGIGPSMQALKE
                     EWETLKNNDYALKFVYPTEKQKNTLKLINKDMSVQERNRRRKLTHNIQEIIHAINSGEL
                     PTELIPEELINILKKSGH"
                     /name="ATP-binding domain-containing protein"
     gene            8766..9437
                     /locus_tag="I6J45_RS18230"
                     /old_locus_tag="I6J45_18225"
     CDS             8766..9437
                     /locus_tag="I6J45_RS18230"
                     /old_locus_tag="I6J45_18225"
                     /inference="COORDINATES: protein motif:HMM:NF021564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2290 domain-containing protein"
                     /protein_id="WP_057095488.1"
                     /translation="MDSDAISKQCTEIIDRLILSGLSIEQSYPSTRMNASGDYEIGFKK
                     GIEISQTLKNIPYEDIYKSVLDEKSYHIHLIDGGLVSFNYIFEKNDDVFFIKKHRLSFY
                     PSPSLPSFEDCPELYSNDEIFLECTKKNLVKFPIRFDFDPKNHVNVTHPASHVTFGQFL
                     NCRIPVSMPVTPRKFILFLLRNFYYSGYTKSKNIFDKKMVNVIPFHTITSEERKIGHFI
                     L"
                     /name="DUF2290 domain-containing protein"
     gene            complement(9558..10721)
                     /locus_tag="I6J45_RS18235"
                     /old_locus_tag="I6J45_18230"
     CDS             complement(9558..10721)
                     /locus_tag="I6J45_RS18235"
                     /old_locus_tag="I6J45_18230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994878.1"
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]; GO:0004024 - alcohol dehydrogenase
                     activity, zinc-dependent [Evidence IEA]; GO:0008270 - zinc
                     ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="WP_005237355.1"
                     /translation="MRALTYHGARDVRVESVPDPVIQEPDDVILRVTATAICGSDLHLY
                     RGKIPATEDGDIFGHEFMGIVEEVGPEVTEVKKGDRVIIPFVIACGHCFFCEHELMAAC
                     ENTNTGRGAILNKKQIPPGAALFGFSHLYGGIPGGQAEYVRIPKGNVGPFKVPGSLPDE
                     KVLFLTDILPTAWQAVNNAQVGRGSSVAIYGAGPVGLLAAACARMLGAEQIFMVDHHPY
                     RLSFAHQTYGVIPVNFDEVDAAEFIIQNTTGYRGVDAVIDAVGFEAKGSMLETVMTNLK
                     LEGSSGSALRQCIAAVRRGGVVSVPGIYAGPIHGFLFGDAFDKGLTFKMGQTHVHNYLP
                     QLLEHIENGDLSPDLIITHRMKLEDAAEGYRIFDKKEEDCRKVILTP"
                     /name="zinc-dependent alcohol dehydrogenase"
     gene            complement(10965..11297)
                     /locus_tag="I6J45_RS18240"
                     /old_locus_tag="I6J45_18235"
     CDS             complement(10965..11297)
                     /locus_tag="I6J45_RS18240"
                     /old_locus_tag="I6J45_18235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032049750.1"
                     /translation="MPKPFLGSADDLAKFLDKHFRLGVKENGSSSFKFLNKGEAYSVLK
                     YVARDVINEEVERLKVEDPKAVARYNPGPSKIRLLDPLKPSTTGYIHIVLKRRYGSGNY
                     CIRPIR"
                     /name="hypothetical protein"
     Typing          3244..4172
                     /name="rep_cluster_1261"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtcttaaaa tcacgaattg aataaaatct tccgcataat tttcttgtct aggcaaatac
       61 gataacgtca acggctgata atattcagcg ttcctcaaat tcgattgtcc tttcttgtag
      121 tgatagccga cagcttgcgt gaacagtcgc ccgacttgat gacgattacc tttgaacaca
      181 gtgtaatgtt gtaaatcggc atttaactgc tgccatttcc cgatgcctac tgatttatta
      241 gggttagaaa tcaaatcatt aataatttct ttgtatttct tctcgattaa atgaacggtt
      301 ggttcgtcat accgccatgt ccaagtgtag tttttctcac tctttttacg atttgaagca
      361 tatttttttg agtttttggt cgagtgtgaa agtcgaacta gctcgacata cttaccattg
      421 ccaaaattga gctttaaata gggtttttga agtacaaatt tgaacttttc ctgatctcga
      481 aagtagtcct ggatgacgct tatttcttgt tgttcattct cacgattcca aacaacattt
      541 tcagcctcag ctactcgttg tccagataga cgtggtttca agtttatttg gctgagctgc
      601 gtgttaaatt tatgagtatt aggcgtagta ataagaagcc aaaaatcaaa aatcgtactg
      661 tttacacgtt tttgcacaat gagatcggca ataggctctt ttttagtgtt cagtcggtat
      721 gatctctgcc tcgaagttag gtctaagtgg tatttatcgg ttaatttctt aatcagctta
      781 tctattttag ggaaaaaaag gttgtcttct ccctcaacag aaaaatgaga ataaaaataa
      841 gcgccatgcc cgaccttatc tacgattttc tgagctgtat aacgactcat tgggctgtag
      901 tgaatcattt tattgtcacc aagcaatatt tataaaattg tcatcaaaca atattacaaa
      961 aacttgattg taatcaagca atatttataa aaagttaaag acttatttta taagattttt
     1021 ataaaaattt agtggcgaag cgacttttgc caatgccgaa ggcattggcc ggacttaatt
     1081 gacacaattt gtcattgtgc aattaagtgg ttcctaagtg acaatattta cacctgtttt
     1141 tacctgctat atgatggaat tattcattga ctgaagcagt ctgaccaact gcctgaccat
     1201 ttcgttttta cgattttgag caggcgtcag cctgcttaaa gccatgtttt tttagatagc
     1261 gcacgttcct ctactagcca ttttgggatg accgattgag cgtactacct ctgaaggggg
     1321 gttcggtacg atctgatggg cgctggagtt tactctttcg atccaatcca agctgtgatt
     1381 tttctttatg acttttcacg gggttgggcg acaaggacac gggagcctat aagcaatcga
     1441 aagattgtga aatggtcgtt aggttagata tagcctagct agatggttgt ttacatcgaa
     1501 aaccctgttc tagcaattac cgtgagaacc tgacaggacg ttaaggttcg ttaataacgg
     1561 ttatgtattg cggctcatgc acgttggaaa caacggatag gcgcagttca taggcacaac
     1621 tgtaggcgtt tgaggtaaca gcgatgttat cggctgcgga gaggaaactt ggaagcagcg
     1681 gcaccactga gtgagatagt ctcaagcaag gtgggtcaac ggatagctca agcagtatac
     1741 atcgggatgc atgaaataat tccataattg cccgagatac gtgtagatca agagccatac
     1801 gctcagattt tttctgattt gccatatccc cattttttta aggaaccgcg ttggggggaa
     1861 actggaagtg caggtcagga gagagagcat tggcggtctg gtcgttaaga ttgctagaaa
     1921 taacttggga atctcttagc tgctgctgtc atggcactgg taggttaaga gagggagaga
     1981 cgatgagtag ggcgtataag gcatataact tgttatatgt tgaagtcgtg gcaaatattg
     2041 ctaaaacaat aggcgaactc aattgcgact ggcagtcgta attggtaaag ggagtctaaa
     2101 ctctatgtga tgtttacgtg gagccgtttc cgaaagtttg agtcggtttt atgttgttat
     2161 ctaaaaaagc gaaaaactcg ttaaagatgg tttggtcgcc atcgttgttt tgcttgggtc
     2221 aggattggtc tcctgactta tgcaagacta aacaacagtc taatgaaaga atgtgatgtg
     2281 atatttagcc acttttagag tgcaacagtc ttgttaatca ccgctctgtt caatagtgcc
     2341 aggtgctatt tctctggata gttttaaagt ttagaaaaac tgatgttagc agacattcca
     2401 atctgtgagt ttttactctt ttttagtaaa gaaagggcgt actgtgatga ttcttaggct
     2461 ctgccgacct aagttattcg ataaacagcg tggtagttac gtcagtgcac tatagccccc
     2521 tcggttttct tcggcaaaga aagcgaaacg aggtgggtgt ctttaatttc taaaatgcga
     2581 tattttgcga ttttaggagc tttctaactt aggacataga atctatgctt aaacgtagta
     2641 aatgagctaa aacccccatt ttttgcgttt aaaagccatt ctcagaacga gtgtctacga
     2701 gtgaacattg aaaagttgcg cctagacttc ctgaaaaaca gttttttcag tatataaatt
     2761 tctaaaaaca gaaaattatg tctccaaagt ctacttcaaa tcgagcgtag cgagcaaaaa
     2821 aagctcatga gcgaagcgaa ttccgagttg cttttgcttt ttctaaaatt cacgaaaata
     2881 ttaactaaaa aattgccccg acgaatcgag cgagagcgag attcaataga gtttgagcgt
     2941 agcgaaaacc aagggcaatt tttcattccc tgtgttttta attattttaa ggtttttaaa
     3001 tgtttttaac ttatttttaa ttattaaatt tcaataacta atctttgtat tcccccacgt
     3061 ttagttaccc attcccccac gtttagttac ccattccccc acgtttagtt acccattccc
     3121 ccacgtttag ttacccattc ccccacgttt agttaccctc ctaattacac ccttaactac
     3181 acagttgtag ttatacccac attgttataa tctgtgggta taaaaatatt tcaatcgagt
     3241 ctatgtcaaa agaattagtt gtaaaaacca atcggttaaa ccaagctttt caaactctat
     3301 ctttatcaga gtttcatatt gttcaattag ccattgttga tgctagacat acgggtactg
     3361 gactaagtac agatactcct ttacgaatag atgctttgag atatgctgaa gtttttggga
     3421 caacaaggca aaatgcttat cagagaatga aagaagcaga ggattcttta tttaacagaa
     3481 gatttagctt ctttgatgaa gatgggaaat tagtcaaaag tagatggatt cagcaagtaa
     3541 aatatctaga tgatgaagga gctattgaac ttgttttcac tttagctgtt gtacaaggca
     3601 taagtaaaat tgatggtatt aaagactttt ttactcaata tttactaagc caaacagccc
     3661 agcttaacag tacatattca gcaagattat acgagttatt aattcaatgg agagctattg
     3721 gaaaaacgcc agtatttgaa ttagcaactt ttagagaaca acttggaatt ggtgttaatg
     3781 aatacaagcg tatggatcac tttaaaacta gagttcttga cttagccatt tctgaaatta
     3841 gcgaaaaaac agatatagag gcaacctacc aacagcataa aaaaggccgt tcaatatctg
     3901 gtttttcttt ttcttttaaa caaaaaaaat ctaaaactaa atcattagaa aatcagacaa
     3961 tatctgggaa tctagatctt ttttcaaaaa aaatgactga ctctcaacgc cacttatttt
     4021 ccaataagct atctgaactt cctgaaatga gtaaatactc tcaaggaact gaaagctatc
     4081 ctcaattcgc agtaagaatt gctgaaatgc ttaaagattc tgagaagtta aaagagtttg
     4141 ctccaatgct tgaaaaggtt ggctatcgtt aaatcattga tgtaacgtta catcactaaa
     4201 tactgtctac tgacatacag acttttactt gggttacatg atgaaagcac tatctgttat
     4261 tgaattagct caactctatg gaatgaatcg tcaaagcatc tataaacgta taaataaagg
     4321 agatttatca aaaaatagtg atggaaaaat agatttttca gaagctctac gtgtatttgg
     4381 tgaaccatcc cacaataata aggatgtaac cacgttacag tctacttcgg tacaaaaaga
     4441 gacagaagta gacatgctta aacaacaagt agacatacta aaaaaacagt tagaacttgc
     4501 acacgaacga gaagtttttc aacgagaaca actagaagca aaagacaatc aaatagaagc
     4561 aatacaacga ctgttagaag ctccaaaaac caatatgact acgtttaccg atcagaaacc
     4621 tagccaagat atagcaacgg atcctcggtc agaacctgag ccgaaacatg acggattgac
     4681 tactccggaa caaacagaaa ataagcggat tccggtgccg gatcacgttg aaccggagca
     4741 gccgaaaaga ggcttttgga gccgtttttt tagaccctat gattaaccac gattgtttaa
     4801 ctaatatgaa taaagtcgtg aaatattgat caaataatag gcgtggaacg tgaaaaatca
     4861 taaaaaaaaa agcccacctt ggggaggttg ggcaaaaaag aaactacagc gttgaatttt
     4921 caagagaatt ataacgcatt tcgtataagg tgtattatgt taattttagg aaatcttaag
     4981 ctttagccaa cataatcttc agttgatatt acttgagcat atgcaaattc aaaagcggcc
     5041 attaatgttg catggacatg accagcaggt actttaacgc cattaaattc caagtcagct
     5101 ctggccctag aatcattttt ttaacttttc ataacgaaga ataaaaataa ctcaatgttt
     5161 actttaggtt ttatacgctt tttcttttca ggagataatg ctaggatcaa aattggctaa
     5221 cattaaataa tcataatgaa gaatcttcaa atagttatag cacaagctct acattcatat
     5281 gaatttgaac atatggagca atcaataagt aaatccatta gcgacactat attggaaata
     5341 ttggtcaatg atgggccttt tgaaaaaatt gattgttatc tgtattcaaa aaaagattct
     5401 aaaaaaattc taaaaagctc tatacactat gtaattgctg tcttagaaag ggcgaagtat
     5461 agagtacaga aagctcagca agataatctg agtcctttgt atatctgctt atacgtgatt
     5521 aagaaaattg agggctttat cgaatactta aatactaaat tttaactgtg aaagcctcca
     5581 tttaacataa tggcgattat acgaagtaca cttgtatatt aatataaata tcagatactt
     5641 aaaattttgg ctctagccaa aaaatgcaca aaaagccgat tttgaaacaa gtcggctttt
     5701 ttatgagcag ttttgaaata ttttgccaat aaatttgact aaaaattacg cttttcagct
     5761 tgagtgatac aattttgcga tacattattg tatatacaaa tttgaatgtt ttttatggaa
     5821 cagtattttg aatgggatga ggctaagaat cgaaagaatc agaaaaagca cgatgtctct
     5881 ttcgaaactg caagccttgt ttttgaagat ccgttgagga tctcaatcca agacagacat
     5941 accgatggcg aagaacgttg gcagaccatt gggaaagtaa aaggcgtact aatgctctta
     6001 gtagctcaca ccatctttga tgaagatgac tgtgaaatca tacgaatcat tagtgcaaga
     6061 caagtaacta aagcagagcg agataaatat gagcatggtt aaatactcac gcaaagaact
     6121 gaatgaaaaa ttcagcgaca agcaagatgc tgaaattgaa cgcttgcttg ctaagggaac
     6181 agtttctgac gatcatctag atctatcaga tatgcctgaa attaccgact ggagtaatgc
     6241 tgtacgccac aatcaattct atcgtccagt gaagcaacaa acatctattc gtttagatgc
     6301 tgacgtactt gcatggttca aagcacaagg caaaggttat cagacacgta tgaatgaaat
     6361 cttacgggat gctatgctaa aagaattaaa aaatcatcaa taaaaatggg agcttaggct
     6421 cccatttttg actgcgtata acgtgtatta tattaatttt agatattttt aataataagc
     6481 aatctctaaa ttgagatgtt taagtttatt tgatttcgaa ctaaccataa gagttattca
     6541 tatcaatgct taacttgata agactaaggc ttataaatat agtagagcat tgcttgattt
     6601 caagatttta aataaaggta ttgctgtgat taaagttatt tggggcacta ataaagaaaa
     6661 accaattgct tcaagacaac ttgcggaaac attatcttct aacatgagtt tagaaggctt
     6721 tctttatata ggttatccaa ttgtaggctc tcctctagga ccgacaaaat ttgatgcact
     6781 acttttaagc gagtcaaaag ggatcattgc ttttgattta gtggaaggaa cggaccttac
     6841 aaactatata aataatcaag atgaaatggc ctccatgttg gaggtgaaat taaaacctta
     6901 tcccaagtta aaaaaaggtc gtcatcttaa gtttgatatt aaaaccgtaa ctttctgtcc
     6961 cgctaaaaaa aataatttgc cacaagtcga ggataatcta tacaacatcg ctaatatttc
     7021 caatctaaat gaagttatca attcatttga gtgggaatca gatgaagata cgtttaaaga
     7081 attaaaagca gcaattcaag ttgttactaa tattcgatct ggtgctatta gaagacaaac
     7141 taaaaaggaa aattctttag gaagtcgcct acaaaaatta gaagattcta tagcgaacct
     7201 agatcagcac cagtctgaag cagtcattga aacagtggat ggtgtacaac ggatcagagg
     7261 gttagcagga tcaggaaaaa ctatcgtatt agcgttaaaa gttgcttatt tacatgcaca
     7321 gaaccaaagt tggaaaatag cagtaacttt ccatacacgt gcgttaaaag aacagtttaa
     7381 aaggctaatc aacaacttta ctattgaaca attaggtagc gaacctgatt gggattctat
     7441 tgatatattt aactcatggg gagcacccgg agataaagaa aacgatggga tgtatcaccg
     7501 atattgtgtt gaaaactcaa tagattacta tgatttccaa acggctaaaa atgagttcgg
     7561 aggggatgat gctttcaaaa atgtttgtaa actagcttta gaacagtcct catctaataa
     7621 aatagaaaaa tatgatttaa ttttgattga tgaagctcaa gacttccctc ctgagttcat
     7681 taagttatgt tataaatttt taaaggaacc taagcgatta gtttatgcat atgatgaact
     7741 tcagtcttta aatggtctgt ccgttttacc tcctgaacag ttatttggga aagattccaa
     7801 aggagtgcca ttagtaacct tagaagaaga agatccaaca aaacctaaaa aagatattat
     7861 tctcgaaaaa tgttaccgca attcacgtcc actacttgca accgctcata cactagggtt
     7921 tggattaact agaccaaaag gattaattca attttttgat aatgattctc tatgggaaga
     7981 tgttggttat tcggttaaaa aaggtaaaat tgaaggtgga aaagaagtag agttaattag
     8041 aactagtgaa tcaagccctc tatttttgga agaacataca cctcttgaag aacttataaa
     8101 atttgaggta tttgattcag ttcgagatat gaatcaaatg cttttcgatt ctattgtcaa
     8161 aaatatccat gaagatgagc ttaagcctga agatatctta gttattaatc ctgacccttt
     8221 cacaacacag aaaaacgtag ggattgtaag aaaagcatta caacaaaatg agatagattc
     8281 tgaaattgca ggtgtcacaa cctccagaga tatcttcaga aaaaatggat ctgttacttt
     8341 tacaggtatc ttcagagcaa aaggtaatga ggctgcaatg gtctacatta tccacgctca
     8401 agattgtgca gattcctatg atccaaatca cttagcacta atcagaaata gattatttac
     8461 tgctattact cggagtaaag cttgggttcg tgtcttggga attgggccat ctatgcaggc
     8521 tctaaaagaa gaatgggaaa ctctaaaaaa caacgattat gctttaaagt ttgtttatcc
     8581 gacagaaaag cagaaaaata cattaaagtt aattaataaa gatatgtctg ttcaagagag
     8641 gaatagacga agaaaactga cacataatat tcaagaaatt atacatgcta tcaattcagg
     8701 agaattacca actgaattaa ttcctgaaga attaattaat attttaaaaa aatcagggca
     8761 ttaagatgga ttctgatgct atttctaaac aatgtactga aatcattgac cgccttattt
     8821 tatctgggtt atcaatagaa caaagctatc catccacacg tatgaatgcg agtggagatt
     8881 atgaaattgg atttaaaaaa ggtattgaaa tttctcaaac gctaaaaaat atcccttatg
     8941 aagatatata taagtctgtt ttagatgaaa aaagctatca tattcacctg atagatggag
     9001 gcttagtttc ttttaattat atttttgaga aaaatgatga tgtttttttt ataaaaaaac
     9061 atagattgtc attctatcct tcaccatcat tgccatcctt tgaagattgc ccagaacttt
     9121 atagtaatga tgaaattttc ttagagtgta ctaaaaaaaa tttagttaaa tttcccatta
     9181 gattcgactt tgatcccaaa aatcatgtga atgttactca tcctgcatcg catgtaacat
     9241 ttgggcaatt tctcaattgt agaatacctg tttcaatgcc agttactcca agaaaattta
     9301 ttctattttt acttcgtaat ttttattatt ctggctatac aaaaagtaag aatatttttg
     9361 ataaaaaaat ggttaatgtc attccatttc atactattac tagtgaagag agaaaaattg
     9421 gtcacttcat tttatagttc acctatactt tatttaacat aaaatctctt atatgcaatt
     9481 cagttgtaag cccaaatgga aatgtccgat ttctccaaag taaaaaagcg cctgatggcg
     9541 ctttccttta atcatattta tggcgtcaga ataactttac gacagtcttc ttctttttta
     9601 tcaaaaatac ggtaaccttc cgcagcatct tccagtttca tgcgatgggt aataatcaga
     9661 tctggagaca gatctccatt ttcaatatgt tctagcaact gcggcaagta attatgcaca
     9721 tgagtttgtc ccattttaaa ggttaaacct ttgtcaaagg catcaccaaa caggaagccg
     9781 tgaattggac cggcataaat tccaggtaca ctcaccacac cgccgcggcg gaccgctgca
     9841 atacattgtc ttaacgctga accactcgaa ccttctaatt tcaggttagt cattacggtt
     9901 tccagcatgc tgcctttggc ttcaaaaccg accgcatcaa tgacggcatc gactccacga
     9961 taacccgtgg tattttgaat gataaattca gcggcatcga cttcatcaaa attgaccgga
    10021 atcaccccat aggtttgatg tgcgaaactc agacgataag gatggtgatc gaccataaaa
    10081 atctgctcgg caccgagcat ccgcgcacag gcagcagcca gcagaccaac tgggccagca
    10141 ccataaatcg ccacactgga acctcgacca acttgggcat tgttcactgc ttgccaggcg
    10201 gttggtagaa tatcggtcag gaataatact ttttcatcag gcagggagcc tggcacttta
    10261 aatggtccga cattgccctt ggggatccgc acatattcag cttgaccacc cggtatacca
    10321 ccatatagat ggctaaagcc aaataaagct gcccctggtg gaatctgttt tttattgaga
    10381 attgcaccac ggccggtatt ggtattttca caggcagcca tgagttcatg ttcacagaaa
    10441 aaacagtgac cgcatgcaat cacaaaggga ataatcaccc ggtcaccttt tttgacttcg
    10501 gttacttcag ggccgacttc ttctacaatt cccataaatt catggccgaa aatatcaccg
    10561 tcttcagtgg ccgggatttt cccccggtat aaatgcagat ctgagccaca gatggcggtg
    10621 gctgtcaccc ttaaaataac gtcatctggt tcctgaatca ctggatctgg aacggattca
    10681 actcgtacat cccgagcacc atgataggta agagcacgca tgcggatttc ctctttctag
    10741 ttcaacatta aacagactcg tatcaaggct gccaaaaata aaaaacactt ttattagaga
    10801 cttatgcgcc aagaaatcgc tgtaaattaa tgtatgaaat aagacatccg agtgtttaaa
    10861 aaataaattt atatagggtt tacgtaattc gtgatcttat gtgtcgaatg aatagaaaag
    10921 aacagaatta aagaaacacc tagattgtta gaacccattt ctagctatct tataggccta
    10981 atgcagtagt ttccgctgcc atatcttcgc ttaagtacta tatgtatata ccctgttgta
    11041 gaaggtttaa gaggatccaa taatctaatt ttagaaggtc cgggattgta acgagctact
    11101 gctttcggat cttcaacttt taaacgctct acttcttcat tgataacatc acgggccaca
    11161 tattttaaaa cgctataagc ctctccttta ttcagaaatt tgaaagagga acttccattt
    11221 tcctttactc ctaaacggaa gtgcttatct aaaaatttag ctaaatcatc agcagaacct
    11281 aaaaaaggtt ttggcataat cttctcctta aactaatcct tatattattc agcattagaa
    11341 ctgcaaactt tattaagtat caatccatta acagttttaa tttaaataag atattgatta
    11401 atcaattgat tataattttc ttcatgcaca ttttccttac caaactctcg tcctgtttct
    11461 tcaaaaaga
//
