LOCUS       NZ_CP069507             9084 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter pittii strain FDAARGOS_1215 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP069507
VERSION     NZ_CP069507.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357357
            Assembly: GCF_016889345.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1649.01x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:49:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,670
            CDSs (total)                      :: 3,575
            Genes (coding)                    :: 3,515
            CDSs (with protein)               :: 3,515
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 23 of 60
            Pseudo Genes (internal stop)      :: 18 of 60
            Pseudo Genes (multiple problems)  :: 11 of 60
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069507.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1215"
                     /culture_collection="DSM:9307"
                     /db_xref="taxon:48296"
                     /plasmid="unnamed3"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            complement(join(8230..9084,1..306))
                     /locus_tag="I6J45_RS18135"
                     /old_locus_tag="I6J45_18135"
     CDS             complement(join(8230..9084,1..306))
                     /locus_tag="I6J45_RS18135"
                     /old_locus_tag="I6J45_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_000179382.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIEFWQAADSHERANAKAYMEYEIALPNEFTPEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHENYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENHPFSSLEVMYYLNYLIKNAKNKEKLEKDFSEIIKIQEPILHQEGWNLNNMNRKLRDS
                     EHNSTPRFEFEVHSQPNYPLPMQNKPPQPEQPKPKSDDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            613..1389
                     /locus_tag="I6J45_RS18140"
                     /old_locus_tag="I6J45_18140"
     CDS             613..1389
                     /locus_tag="I6J45_RS18140"
                     /old_locus_tag="I6J45_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000191151.1"
                     /translation="MTQEKAKKRGRPAQLLQMAELHAFVEFLLEKDPRSELQNQVIAAL
                     QAEDFNFEMLSEAQQILVKEALKPYREHLKLQLLFDELVITPGRTEYEEKFLELYQDYK
                     KDDLDPAEMNILKTMCTRYLNFKAQKLEYSDLELYLSQIQKKYAGKKRKAENQRKFEVG
                     GGVLSLYKVKDDENLDSKKFLKRIEIDRFLAGKFRQSQLYKKVWALERPNTEKYQLLFE
                     VLHQLSGYSLKGQTLTLLDLVIINAQKKIKSRSEKE"
                     /name="hypothetical protein"
     gene            1614..2813
                     /locus_tag="I6J45_RS18145"
                     /old_locus_tag="I6J45_18145"
     CDS             1614..2813
                     /locus_tag="I6J45_RS18145"
                     /old_locus_tag="I6J45_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005804948.1"
                     /translation="MTPKILCIKSLELEVEKIMSIQEFAFQLSKKIQEQHSVKISRSHI
                     YELIALDQDYRTYSSFVAQNLLLDAQYDDSEEYYQHELVDALTLEILENPPETDYLNYS
                     EDDLHWDDYEGNVLLEQIRKIIIRLKVLLKLDASEEFYLSISKTVYRELLWLNLEVINF
                     NAVREELSYINFENGKVDDLELGGDYFEFSKIGAIFEKILSYAQDRENADAYALLGAYY
                     RYLANQIAPYGRHGSNFGSRWDNNKQKYINSDETKKNKEKYEEYIKQAEHFESYIKNSP
                     MNLHEINLDADKETVYNQILYLCNRGDTSAIESFLYEEIFKNHGEAWLYIYLAQMCGVD
                     FTQDDFRDYNAYTGEDYDDYGPIEIAGREAIQYAIDLSELDVDKDNLARKIAAELFEKI
                     "
                     /name="hypothetical protein"
     gene            complement(2886..3128)
                     /locus_tag="I6J45_RS18150"
                     /old_locus_tag="I6J45_18150"
     CDS             complement(2886..3128)
                     /locus_tag="I6J45_RS18150"
                     /old_locus_tag="I6J45_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001015364.1"
                     /translation="MNHFVQPKLLISSQAKKIGLALINDLELCITARLAFYLDVNPSYD
                     GHEATFIWIEYFLQDHPQAEFSEVREAFLQLIPGC"
                     /name="hypothetical protein"
     gene            3277..3501
                     /locus_tag="I6J45_RS18155"
                     /old_locus_tag="I6J45_18155"
     CDS             3277..3501
                     /locus_tag="I6J45_RS18155"
                     /old_locus_tag="I6J45_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002072934.1"
                     /translation="MPYEEFQRLIGKSGLSIKEFAALLDMNANSITNYKKNGKVPTTIA
                     VIAVVISDMKDDGLDFYPIFEKVRAYSDQ"
                     /name="hypothetical protein"
     gene            3717..3992
                     /locus_tag="I6J45_RS18160"
                     /old_locus_tag="I6J45_18160"
     CDS             3717..3992
                     /locus_tag="I6J45_RS18160"
                     /old_locus_tag="I6J45_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140357.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_005140357.1"
                     /translation="MNTLAKKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQV
                     VNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQELFTDLEDDTDD"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            3976..4266
                     /locus_tag="I6J45_RS18165"
                     /old_locus_tag="I6J45_18165"
     CDS             3976..4266
                     /locus_tag="I6J45_RS18165"
                     /old_locus_tag="I6J45_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_000176412.1"
                     /translation="MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTV
                     PDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDNNELILARIGSHSELFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(4314..4460)
                     /locus_tag="I6J45_RS18170"
                     /old_locus_tag="I6J45_18170"
     CDS             complement(4314..4460)
                     /locus_tag="I6J45_RS18170"
                     /old_locus_tag="I6J45_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000192351.1"
                     /translation="MTQKNSNTSTNPANQQPHKDIKSEVPPPPVRYGLDEKIKAEVPKS
                     NKK"
                     /name="hypothetical protein"
     gene            complement(4457..4978)
                     /locus_tag="I6J45_RS18175"
                     /old_locus_tag="I6J45_18175"
     CDS             complement(4457..4978)
                     /locus_tag="I6J45_RS18175"
                     /old_locus_tag="I6J45_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000999915.1"
                     /translation="MNDSDDRNHKVILEYLQKVLERERDRFYKLDDKVSKLFTTVSTVI
                     TGFLIIVNILLDHNKISIFCSSEITILSLLSLTTFCFLCMSWNFLFKVFNLKKTAHLQH
                     QGFWKYYKNREEIDFYERIIKDHETVIDTYIESNDQKVLLLKKSLSNIYLSAIMFVILV
                     IIYLIFKYLK"
                     /name="hypothetical protein"
     gene            complement(5503..5820)
                     /locus_tag="I6J45_RS18180"
                     /old_locus_tag="I6J45_18180"
     CDS             complement(5503..5820)
                     /locus_tag="I6J45_RS18180"
                     /old_locus_tag="I6J45_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065053.1"
                     /translation="MLGENHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            complement(6171..6737)
                     /locus_tag="I6J45_RS18185"
                     /old_locus_tag="I6J45_18185"
     CDS             complement(6171..6737)
                     /locus_tag="I6J45_RS18185"
                     /old_locus_tag="I6J45_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000858429.1"
                     /translation="MKTSTKYSVSDAATLYKKSRATLYKDIKNGVLSRDHDGSIDFSEL
                     LRVYGEPFSGKHSKHTDTSPTQTINTPEYTDNTVIELKNQIDFLKKQLEKAEDREAKAN
                     SRIDTLLTLIEMKPTQVHPTPTSDQSSVQSSPSQVEPTLNVDNDKHDGLTTPEKTENKR
                     IPVPEPVEPEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(6801..7682)
                     /gene="repM"
                     /locus_tag="I6J45_RS18190"
                     /old_locus_tag="I6J45_18190"
     CDS             complement(6801..7682)
                     /gene="repM"
                     /locus_tag="I6J45_RS18190"
                     /old_locus_tag="I6J45_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734502.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005804946.1"
                     /translation="MNKNHVVKSNQVIEASYQLSAVEQRIVLAAISRIPKNQPITDDEL
                     YPVSVDELKQLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEIQFLDSQSV
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVESL
                     RTMLELGKKYPLFADFKKRVIDTAIDQINEYSPLKVTYEQKKTGRKVTHIIFSFKEKSK
                     SITHTSKDFYQLSDAQINLFGNQLSRLHELSHLASEGESYEILAAKIKDMLRNPHQQQQ
                     FLPHLKNLGFKA"
                     /name="repM"
     Typing          complement(6804..7682)
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.922"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..306)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.353"
                     /coverage="100.0"
                     /evalue="1.47e-49"
     Typing          complement(8300..9084)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.565"
                     /coverage="100.0"
                     /evalue="2.13e-167"
ORIGIN
        1 tatgtgtttg tctaaaaacg tctctatgag cgttttacgc tgttctggcg tgaactcgtt
       61 ggggagggca atttcatact ccatgtaggc cttggcattg gctcgttcat gactatcggc
      121 tgcctgccaa aactcaatcg gatctttgac aaaggcgggc atgcaacgtc caggttcaat
      181 atgttctatg gtttcattgc ttgtttttcg aatgccttca tattgcatca gccgggaaat
      241 atagaggtag tggccggtcg catagccttt atttttgctt tttacactga aatggtagct
      301 ggccatgttc acctctggtg gcttggggac cggtttttat gcagtgaaac ggaatgaaaa
      361 ccgtccggat tttgaaatgc ctgaatggct gagaaatccg gttaaagggg gtcaaggggg
      421 aatcgcccca ttgcgtacta gtttcttcga ttagaagaaa ctgtcaagtg cgaattattt
      481 aaattaaaaa taaaatttct tttcaagaaa ttttattttt aatttaaata attattttca
      541 gactgcccga tttaaatatg gtaaaaccat atttaaatcg gtagcagttc tgaaaattag
      601 gcggcagatg atatgaccca agaaaaagca aaaaagcgtg gtagaccagc tcaactttta
      661 caaatggcag agttacacgc tttcgttgag ttcctgttag aaaaagatcc tcgctctgaa
      721 ctgcaaaatc aggttattgc tgcactacaa gctgaagact ttaattttga aatgttgtca
      781 gaagctcagc agattctggt gaaagaagcg ttaaaaccgt accgtgagca tttgaaactt
      841 cagttgttgt ttgatgagtt ggtgataact ccaggaagga ctgagtatga agaaaaattc
      901 ctggagctgt accaagatta taaaaaagat gatctagatc cggctgaaat gaatatcctg
      961 aaaacgatgt gtacgcgtta tctgaatttt aaagcgcaaa agcttgaata cagtgacttg
     1021 gagctgtatt tgagtcagat ccagaaaaag tatgccggga aaaagcgaaa ggctgaaaat
     1081 caacgtaagt ttgaagtggg tggtggggtt ttaagtcttt ataaagtaaa agatgatgaa
     1141 aatttagaca gtaaaaagtt tctaaaaaga atcgaaattg accgttttct tgccgggaaa
     1201 tttcgtcaat ctcaacttta taaaaaggtt tgggcactag agcgaccaaa tacagaaaag
     1261 tatcagctac tttttgaggt tctccatcag ctgtctggat atagcctaaa agggcagact
     1321 ttaacgcttc tcgatttagt gattataaat gctcaaaaaa agataaaaag taggtctgaa
     1381 aaagaatgaa ttttttgact gtccgctttt tagctgactt ttctattttg gcaattgtaa
     1441 atttatgcga aatttattat tttaaaaata aggtcgccta ccgtacttac gcatgagtag
     1501 aaaagggata aaaaaccata ggtttaattt ttaaagccat atctaaaatc taattttctt
     1561 aagtatcaac tcgtttggtt cttagcattt aaacatgcgt tgctagaagt cttatgacac
     1621 caaagattct ttgtataaag agtttagagt tggaagtaga gaaaattatg tctattcaag
     1681 aattcgcatt tcaattatct aaaaaaattc aggaacagca ttctgtaaag attagccgtt
     1741 cacatattta tgaattaatt gctttagatc aggactatag gacctatagc tcttttgttg
     1801 ctcaaaatct tttacttgat gctcaatatg atgattctga agagtattat cagcacgaat
     1861 tggttgatgc tctaactcta gaaatattgg aaaatccgcc tgaaacagat tatctaaatt
     1921 atagtgaaga tgatcttcat tgggatgatt atgaaggtaa tgttctattg gagcagatta
     1981 gaaaaattat tataagactt aaggttttac ttaagttaga tgcctcagaa gagttttatt
     2041 tgtcgatttc taaaacagtc tatcgagaac ttttatggct taatttagaa gttattaact
     2101 tcaatgctgt aagagaggaa ttaagttata tcaattttga aaatggtaag gtggatgacc
     2161 tagaacttgg aggtgattat ttcgagtttt caaaaattgg ggctattttt gagaagattt
     2221 tatcttatgc gcaagatcgt gagaatgctg atgcttatgc tctactcggg gcctactatc
     2281 gttatttagc aaatcagata gccccttatg gtcgacatgg ctcaaatttt ggaagtcgtt
     2341 gggacaataa taaacaaaaa tatatcaatt ctgatgagac taagaaaaat aaagaaaagt
     2401 atgaagaata cataaagcaa gcggaacact ttgaatctta tattaaaaat tcacctatga
     2461 atcttcatga aattaatttg gatgcagaca aggaaacagt ttacaaccag attctgtact
     2521 tgtgtaatcg aggtgatacg agtgctattg aatccttcct gtatgaagaa attttcaaaa
     2581 atcatggaga agcatggtta tacatctact tagctcaaat gtgtggagtt gatttcacgc
     2641 aggatgattt tagagactac aatgcttata caggtgagga ttatgatgac tatggaccta
     2701 ttgaaatcgc aggtcgtgag gcaattcaat atgctataga tttatcagaa ctggatgttg
     2761 ataaagataa tttagctagg aagattgctg cagagttatt tgagaaaatt taattcatag
     2821 tcttataaaa aagagaattt gaaatctgta tttgactcat aaaatacaga tttcattatc
     2881 atcgtttaac atcctgggat aagttgtaga aaggcttctc taacttcaga aaattcggct
     2941 tgaggatgat cttgtaaaaa atattcgatc caaatgaatg ttgcctcatg gccgtcatag
     3001 gaaggattta catccagata aaaagccaat cgtgctgtta tacaaagttc aagatcgtta
     3061 attaatgcca atccaatctt ttttgcttga gaagaaataa gaagcttggg ctgtacgaaa
     3121 tggttcataa aaacaagtcc tatcaaaaga gtaaaattgc ttgataagac catttaaaca
     3181 cataaataat gtgttaaaca cattatttat gtgtttaaat gtaaataaat gatctgtgct
     3241 atacttttcc atcagaacaa ttctgctttt aaaactatgc cttacgaaga atttcaaaga
     3301 cttataggta agtctggcct ttctattaaa gaatttgcag cgcttttaga tatgaacgca
     3361 aattcaatta ccaactataa aaagaacggt aaagtgccca ctacgattgc agtaattgca
     3421 gtcgtaattt cagatatgaa ggatgatggt ctggactttt atccaatttt tgagaaagtt
     3481 cgagcttata gtgatcaata gagtatttga tgtgaaccca attaaagatt taattggatc
     3541 ctagcacgtg ctgctgcatt tcgtagatga gattttatgt taagttagtt gttgtgtagc
     3601 acatgtgtgc tacaatggct ttagccaaat caatgaactg actactgaac tgactactga
     3661 actgactact gaactgacta ctgaactgac tactgaactg actactggat aaacatatga
     3721 atactttggc aaaaaaagat gagtatgtac gtgctagggt tccaaatgaa ttaaaggttc
     3781 aagcggagcg aattctaaaa gaagtgggtt taaatactac agatgctatt cgcttattat
     3841 taacgcaagt agtaaatcga ggtggatttc ctttagagtt aagaacccct aaccgtacaa
     3901 caattgaggc tatgaactct gttgcagaac ctaaaaaatt tagttctgct caggaattgt
     3961 ttacggattt agaagatgac actgacgatt aggtacaaga aacaatttaa aaaagatttt
     4021 aaacgggtaa gctcaaaccc aaagcttaag aaaggactct taattttgaa ggatcttatt
     4081 gatgagcatt tggtaaaaac agggactgtt ccagatgagt atttacctca tcctttgatt
     4141 ggtgagtgga aaccccatat ggaatgccat attttacctg atttattatt aatttgggat
     4201 gtggatgaag ataataatga gctaatactt gctcggattg gatctcattc tgaacttttt
     4261 ggataagtaa aaagcccgat atattcgggc tttttgttgt caataattag agattatttt
     4321 ttattacttt ttggaacttc agctttaatt ttttcatcta aaccatatct aacaggagga
     4381 ggtggaactt ctgatttgat atctttgtga ggttgttgat tagctgggtt ggtacttgta
     4441 ttggaatttt tttgggtcat tttagatact taaaaatcaa atagataata actaatataa
     4501 caaacattat tgctgataaa tagatgttgc ttaaagactt ctttaaaagt aaaacttttt
     4561 ggtcattaga ttctatgtat gtatctataa cagtttcatg atccttaatg attctttcat
     4621 aaaaatctat ttcttctcta tttttataat atttccaaaa cccttgatgt tgaaggtgag
     4681 ctgttttttt taaattaaaa actttaaaaa ggaagttcca agacatgcac aagaagcaga
     4741 atgttgtaag tgacaataaa cttagaatag taatctcgga agaacaaaaa atactaattt
     4801 tattatgatc taaaaggata ttaactataa ttagaaatcc tgtaatcaca gtagatactg
     4861 tcgtaaataa cttagatact ttgtcatcta atttataaaa tcgatctcgc tctctttcta
     4921 gtactttctg tagatactca agaattacct tatgattacg atcatcagag tcattcatac
     4981 tcaatcccct ttatttcaaa taacaaaatt tatttaattt aaattttaac aataaaaaat
     5041 cccgacatca tgtcgggatt tttcggtgtg gagcgcctag gtagggctga ctacctgatg
     5101 gtcatggtgc ctgactgatt attattgtta ttattgttgt tttaagtcat tgaacatctt
     5161 gttcatatag attgatcata aggacttcat cgggggatag atacagacaa tttcgtaaaa
     5221 cgctgtaagc caaaacgtac aaaaaataat caaaatttat cccaatggtt atccgcataa
     5281 attgtggatt cttttgggta aaaaaggctt aaaaactcta tggaattgct atttttgatc
     5341 attatttaac caattttatt ggcaagaagt atcaaccttg ctcaaaaaaa atccgctttg
     5401 gaaacaaagc ggatttttgc ggtagtttcg ggttatgtaa aatagctaag tacatgattt
     5461 tatttaactt ataaaagacg atttcggata acgcccatta tgttaaatgg aatgtttatg
     5521 agaaattaat agtctatttt tgtttgagat tgaagtatat ttaaaaaagt taagttgttt
     5581 ttttaaatgc ataacttcat cttttagttt tttgatttgt tgctctcttt cgtcctgtat
     5641 agggtaagta agttcaccaa attttctaac taactgactg tattcaatca atttattgta
     5701 gtcaagttcg aaatctccgc tttttatata tttaagtata gtcgagcgag gttttttata
     5761 cgctatagca gcatcactga tcgaaaattt ttttaaaaaa tgatgatttt cacctagcat
     5821 actatttcac ttaaggcatt attaatttcg tatatatatg ttaataaaat taacacatac
     5881 aagatataaa tctttaatca ttattggtta ataatcgaac gtttacttta cacatgccct
     5941 actctgataa tttttctatt attaaatcaa tatgctttta aaaatttata ttattaacat
     6001 aatacacctt atacgaaatg tgtcataatt taccttgaaa ttcatatact tatttcaaag
     6061 tttatagccc gaacatccct gtacgggctt ttttttgtga tctttcacgt aatatggcta
     6121 ttctttaatc aaatccttac aactttgatt agctcagttg aatagtaccc ttagccattc
     6181 ggcaagaaaa accggcttaa ccaccctctt ttcggctgct ccggttcaac cggctccggc
     6241 accggaatac gcttattttc cgttttttcc ggagtagtca atccgtcatg tttgtcgtta
     6301 tctacattca atgtaggttc tacttgagat ggactagatt gcactgaaga ttgatctgaa
     6361 gttggtgtgg gatgaacctg agttggcttc atttcaatta gggtaagtaa tgtatcaata
     6421 cgagaatttg ctttagcttc gcgatcttct gctttttcta actgtttctt tagaaaatcg
     6481 atctgattct ttagttcaat aactgtattg tctgtgtatt caggtgtatt tatagtttgt
     6541 gttggagacg tatcagtgtg ttttgagtgt tttccactaa aaggttcacc ataaacgcgg
     6601 agtaactcag agaagtcgat agagccatca tgatcacgac ttaacacccc attttttata
     6661 tctttataaa gtgtagcacg agatttctta tacagtgtgg ctgcatcact aacactgtat
     6721 tttgtacttg ttttcatagt gtattgctgt gtatttagga tacgtattga agaatacaat
     6781 aagtgtattc ttcaatacac tcaagcttta aaccctaagt tctttaagtg tggcaaaaac
     6841 tgctgttgtt ggtgagggtt tctaagcata tccttaattt tagcagctaa aatctcataa
     6901 ctttcacctt ctgaggctaa atgggaaagc tcatgtaaac gtgataattg attcccgaat
     6961 agattaattt gagcatcaga tagctgataa aaatctttcg aggtatgtgt tatggattta
     7021 gatttttctt taaaagaaaa gataatatga gtgactttac gccctgtttt tttctgctca
     7081 taagtgactt ttaaagggct atattcattg atttggtcta tagcagtgtc aattactcgt
     7141 ttcttgaaat cagcgaataa tgggtacttt ttaccgagtt ctaacattgt ccgtaaactc
     7201 tcaacagaaa tttcacgctt acctatttgc cgatattgaa ccagtagctc ataaatacga
     7261 ataccgtatc cactattgat cccagcaatg tctgataagg catatttggt gaattctcta
     7321 cttagattgg aaatgaatgg cagaatgggt tttgaaaaac ggattccaat gacactttga
     7381 ctatctaaaa attggatttc ttgcacccat ctcattttta tagatttatc atcaatactg
     7441 aggttaatag acctctcata gagtctatta ataccttcct ttaaatctct gtatgcagtt
     7501 ttctcatgta cgcccaattg ttttaattca tcgacgctaa cagggtataa ctcatcatct
     7561 gtaataggct gatttttggg aatccgtgaa atggcagcta agacaattcg ttgctctacg
     7621 gcacttaatt gatacgatgc ttctattact tgatttgatt ttacaacgtg atttttattc
     7681 aaaacaaaac cattgatttt tctaatatat gacaaatata tacttgtcat agttagtagt
     7741 caatccgtca tagttaagta gtcaatccgt catagttagt agtcaatccg tcatagttag
     7801 tagtcaatcc gtcatattta aacgttgaaa cctttgctat ataaggcaca gagacgactg
     7861 aaaagattta aaagatttaa aatatattaa aaaggaaaga atgaaaaatt gccctatgtt
     7921 ttcgctacgc tcaaactcta ttgaacttcg cttacgctca gttcgaaggg gcaatttttg
     7981 ggttaatctc gtgataactt caaaaaagca aaagcaactc ggaattcgct tcgctcataa
     8041 gttttttata ctcgcttcgc tcgacctaga tgcaaattaa atctgttcgc accctccggg
     8101 atgctctgta tctcagtctc agcagcaaag gggagtttgt aatcagggaa aaagcatggc
     8161 gaaaatttta attgggtctg ttcgctcgta gacactcgct ctgtaaggcc tgaataaagc
     8221 cagataattt taaaaaccag gaccaccgta atcataatca tcagattttg gctttggctg
     8281 ttcgggttga gggggcttat tttgcatggg taatggataa tttggctggg aatggacttc
     8341 aaactcaaag cgtggcgttg agttatgttc tgaatctctc aattttcgat tcatgttatt
     8401 caggttccag ccttcttggt gcagtatagg ctcttgaatt ttgataattt cactaaaatc
     8461 tttttccagt ttttccttat ttttcgcatt tttgattaag taattcaggt aatacatcac
     8521 ttccaatgat gaaaatggat ggttttcagc actataaaaa tattcatgtt gttgatctaa
     8581 ttgcttttgg cgttcttcga gttctcgttc ttgctggttg tgttctcgga ttttttggat
     8641 ttgagcgaga tattccgctg tcgcattttg ttggccaacg cctagttttg gctctggaag
     8701 gtatagcgtg ctgtggatct cgttatattt ttcatagctg tctgctgaaa cctgattttt
     8761 gatggtgatg tcttggccat ccagtgtgta ggtgacggtc gggcagtgct gtgcaagggc
     8821 atcattcaaa tgattttccc attgttttcg gatatagatc aaaaaagtgg aatagttttc
     8881 atggccatct tgaagttgga tttttttagc accgcctttg gctggatctt gagggttata
     8941 gcgtttgaag tactgttctg cgctgcgttc aatgccgtca tccgctttta gcgagaacat
     9001 caggtgacaa tgaggctgat cttcgccact gatgcgtgat tttacgttgt gtatggcgta
     9061 tgagtgagga tattgctgtg ggac
//
