LOCUS       NZ_CP069505            11158 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter pittii strain FDAARGOS_1215 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP069505
VERSION     NZ_CP069505.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357357
            Assembly: GCF_016889345.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1649.01x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:49:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,670
            CDSs (total)                      :: 3,575
            Genes (coding)                    :: 3,515
            CDSs (with protein)               :: 3,515
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 32 of 60
            Pseudo Genes (incomplete)         :: 23 of 60
            Pseudo Genes (internal stop)      :: 18 of 60
            Pseudo Genes (multiple problems)  :: 11 of 60
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069505.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1215"
                     /culture_collection="DSM:9307"
                     /db_xref="taxon:48296"
                     /plasmid="unnamed1"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            complement(join(10915..11158,1..1244))
                     /locus_tag="I6J45_RS17600"
                     /old_locus_tag="I6J45_17600"
     CDS             complement(join(10915..11158,1..1244))
                     /locus_tag="I6J45_RS17600"
                     /old_locus_tag="I6J45_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953322.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005065066.1"
                     /translation="MLSTIREQWFSNIRGDVLAGIVVALALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVIAFVGGRPGMISGATGAMALLMVTLVKEHGLQYLLAATILTGFIQILAG
                     YLKLGALMRFVSKSVVIGFVNALAILIFMAQLPELTNVTWHVYAMTIGGLAIIYLFPYI
                     PVIGKLLPSPLICIVLLTLFALFIGLDVRTVGDMGQLPDTLPIFLLPDIPLNLETLTII
                     LPYSLGLAAVGLLESMMTATIVDDLTDTNSDKNRECKGQGVANIASGFLGGMAGCAMIG
                     QSIINVKSGGGTRLSTFSAGVFLLIMVVFISDWLKAIPMAALVAVMIMVAIGTFNWDSL
                     RNIRQYPLSSNIVMIVTVIVVVATHNLAYGVLVGVLLSALFFANKIERYMAIQSEFNEP
                     ENTRTYTVTGQVFFSSADKFTSAFDFKEALSKVVIDVSQAHFWDVSAVAAVDKVVIKFR
                     REGTEVELIGMNEASKTLVDKFGIHDKPDALDRLNSH"
                     /name="SulP family inorganic anion transporter"
     gene            1757..2044
                     /locus_tag="I6J45_RS17605"
                     /old_locus_tag="I6J45_17605"
     CDS             1757..2044
                     /locus_tag="I6J45_RS17605"
                     /old_locus_tag="I6J45_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002124950.1"
                     /translation="MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIISARQVTKAERNQYEHG"
                     /name="BrnT family toxin"
     gene            2031..2342
                     /locus_tag="I6J45_RS17610"
                     /old_locus_tag="I6J45_17610"
     CDS             2031..2342
                     /locus_tag="I6J45_RS17610"
                     /old_locus_tag="I6J45_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_005065064.1"
                     /translation="MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEI
                     TDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQGKGYQTRMNKILREAMLEDLKNH"
                     /name="BrnA antitoxin family protein"
     gene            complement(2433..3107)
                     /locus_tag="I6J45_RS17615"
                     /old_locus_tag="I6J45_17615"
     CDS             complement(2433..3107)
                     /locus_tag="I6J45_RS17615"
                     /old_locus_tag="I6J45_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_005065062.1"
                     /translation="MSQKILQRYEQLLTDSASIKAMTKKAYSEYSGSYDTLGDEGDALY
                     LEWKVKVKNLLLLSCGEHSIHYRDFLDAEETQSFDTNTRIISRLIPILKASYDDFKNGF
                     LTSFKQIVQAEVFDSELEQARSLLSSGYKNSAAVIAGVVLETAIKELCLNNGIELEKKK
                     LTRLNEDLAKAGVYNPLQQKLITAMADIRNNAAHGDYDQFTKEDVHRMIEDIERFLLAY
                     SS"
                     /name="HEPN domain-containing protein"
     gene            complement(3159..3560)
                     /locus_tag="I6J45_RS17620"
                     /old_locus_tag="I6J45_17620"
     CDS             complement(3159..3560)
                     /locus_tag="I6J45_RS17620"
                     /old_locus_tag="I6J45_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065059.1"
                     /translation="MTDKKQNDHLNLDGINSSYNDGDGLRINNPEDFRSITISNGYFSN
                     NKGNGITIGSPQQSPLEIILTQLAPKLPDTIQPYELASVIQNLLESTNQEEISQKLMTS
                     GLKEKFKDPNLWISFSSLLFSLIFQFSSK"
                     /name="hypothetical protein"
     gene            complement(3652..3954)
                     /locus_tag="I6J45_RS17625"
                     /old_locus_tag="I6J45_17625"
     CDS             complement(3652..3954)
                     /locus_tag="I6J45_RS17625"
                     /old_locus_tag="I6J45_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_005065057.1"
                     /translation="MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3959..4303)
                     /locus_tag="I6J45_RS17630"
                     /old_locus_tag="I6J45_17630"
     CDS             complement(3959..4303)
                     /locus_tag="I6J45_RS17630"
                     /old_locus_tag="I6J45_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_005065055.1"
                     /translation="MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSGKKRFYTEMLAIADKQ
                     YELHLSTLGE"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4824..5141)
                     /locus_tag="I6J45_RS17635"
                     /old_locus_tag="I6J45_17635"
     CDS             complement(4824..5141)
                     /locus_tag="I6J45_RS17635"
                     /old_locus_tag="I6J45_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000900860.1"
                     /translation="MLGKNHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            complement(5491..6021)
                     /locus_tag="I6J45_RS17640"
                     /old_locus_tag="I6J45_17640"
     CDS             complement(5491..6021)
                     /locus_tag="I6J45_RS17640"
                     /old_locus_tag="I6J45_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_005065050.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSQNKIDLAEAIRV
                     FGEPVKKQDVKEPVKIDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPNMTTFTDQELVQPSIAKPDPEPDTAEAKHDGLTTPERRENKWIPVPEHIEPEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(6100..7029)
                     /gene="repM"
                     /locus_tag="I6J45_RS17645"
                     /old_locus_tag="I6J45_17645"
     CDS             complement(6100..7029)
                     /gene="repM"
                     /locus_tag="I6J45_RS17645"
                     /old_locus_tag="I6J45_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005065049.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKGTDTLALFTKMSDKQRHLFANKLSELPEMGQYSEGTESFQQFA
                     IRIAEMLQDYQKFQELFPYLQKVGFAPA"
                     /name="repM"
     gene            complement(7727..8440)
                     /locus_tag="I6J45_RS17650"
                     /old_locus_tag="I6J45_17650"
     CDS             complement(7727..8440)
                     /locus_tag="I6J45_RS17650"
                     /old_locus_tag="I6J45_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065071.1"
                     /translation="MAGRKTFALTQQEIIDLDKHIRALPFKRRMQDAFLAMISERESMK
                     QLNEEDLKLIQRCRYERNTYNKRIVQLQLIQKTEPQKLKKLELNILELSKKTDIDAYFA
                     MHDALNEVLKIKRNQTSEKNITAKVERVLNPEKYNQAPLSKSLRKQQDHIKYFIGSLYL
                     NLLEQSGLERSNSNEDLDRMLDLFKQAQSGAEKNNAFSLDQNRKTDILSAKSLYEMEKL
                     IKQAKEDPRNPFNKA"
                     /name="hypothetical protein"
     gene            8896..9780
                     /locus_tag="I6J45_RS17655"
                     /old_locus_tag="I6J45_17655"
     CDS             8896..9780
                     /locus_tag="I6J45_RS17655"
                     /old_locus_tag="I6J45_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_005065070.1"
                     /translation="MFYLNLKHNIKKEQSSLNAFHYLTRTAHFENQKDNEKLEFMRYGN
                     MPKWAENKPKLFWKSADQFEISRGRTSSTLTIALPKELILEQRAELVQKLIDQFAGQYQ
                     FPYTAVIHNHPSEITGEDQPHLHLMYSERTISDDIERPPEQFFKQYRPKNPTQGGAQKL
                     TADALGFGRNQIKVFREKTQELMNLFLEKYAPTKKVMVYGVEIEVKNQVSCLSNDDFNQ
                     KFGTKLQDVPQIPRWKLYSADPIIQLDVEAQKNTIKKIRNQNNAELYGKEYDLEISRRH
                     VLTQKKDPGFSLD"
                     /name="MobA/MobL family protein"
     gene            complement(10051..10902)
                     /locus_tag="I6J45_RS17660"
                     /old_locus_tag="I6J45_17660"
     CDS             complement(10051..10902)
                     /locus_tag="I6J45_RS17660"
                     /old_locus_tag="I6J45_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034565.1"
                     /GO_function="GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="WP_005065068.1"
                     /translation="MSKIIACIDGSLATNTVCDYAAWFSDKLSSPLKLLHVIDKPKAKA
                     PQDLSGAIGLGSRETLLKELVEIEERKGKIELEHGQILLREAKNYLLEKFSIDAQSFQR
                     HGSLLETIMGMEEDIRVLVMGKHGAETEHDSSKVGTHIENVVRALHKPVLITSSPFSPP
                     KSFLIAFDGSPTARICVDKIANSPLLKTLTAHVVYVGKPNSDMTLQVTWAKELLANNGF
                     DVIDSMLDGDVEKSILDYQEKNVIDMIVIGAYGHSKIRQFFIGSTTTKLLSSSTKPVLL
                     LR"
                     /name="universal stress protein"
     Typing          complement(6114..7029)
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.884"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          8897..9777
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagctgaaga acacttgacc tgttaccgta taagtacggg tattttcagg ttcattaaat
       61 tcggactgaa ttgccatgta gcgttcaatt ttgttggcaa aaaataatgc agaaagaagt
      121 acaccaacta atacgccata agccaaatta tgtgtcgcaa cgaccacgat gactgtaacg
      181 atcatcacaa tattgctaga taagggatat tggcggatat tgcgtagtga atcccagtta
      241 aatgttccaa ttgctaccat gatcatgaca gcaaccagtg cagccattgg aatggcttta
      301 agccagtcac tgataaacac caccataatt aacagaaaga cccctgcact aaatgtagat
      361 aaacgggtac ccccaccaga tttcacatta ataatcgatt gaccaatcat ggcacagcct
      421 gccattccgc ctaagaaacc tgaagcaata ttggcaacgc cttgtccttt acactcacgg
      481 tttttatcac tattggtatc tgttaaatca tcgacaatcg ttgctgtcat catagattca
      541 agcaaaccta cggctgctaa tcccagtgaa taaggcaaaa taatcgttaa ggtttcaaga
      601 tttaaaggaa tatcaggaag caagaaaatt ggaagcgtat ccggtaattg ccccatatct
      661 ccgacggttc ttacatctaa accaatgaat aaggcaaaga gcgtaagtaa gacaatacag
      721 atgagaggag agggtaaaag cttccctatc actggaatat aagggaatag ataaataatg
      781 gctaaaccac caattgtcat cgcatagaca tgccatgtca cattagtgag ttcaggtaat
      841 tgagccataa aaattagaat agccagggca ttcacaaagc caatcacaac tgacttggat
      901 acaaaccgca tcaaggcacc taattttaga tagccggcta agatttgaat aaatcctgtc
      961 aaaattgtgg ctgctaataa gtattgcaga ccatgctctt taaccagtgt taccattaat
     1021 aacgccatgg caccggttgc cccagaaatc ataccagggc gaccacctac gaaagcaatc
     1081 accacggcaa tacagaatga agcgtataac ccgactttag gatcgacacc tgcaataata
     1141 gagaaggcaa ttgcttcagg aattagagcc aaagcgacaa caatacccgc aaggacatcc
     1201 ccgcgaatat tagagaacca ttgttctcga atagtggata acatgaaata gcctattttt
     1261 ttaaatttta agaaaggcta gtgatgtgat atatacgaca cacaagctac tacaccacat
     1321 cactagacag tgatgagtta tttactgatt taagaggtaa ataaacttat ggtggcgtaa
     1381 gtgtcatatt aagaaagata tctattttgg aggatttcgg gggcaatcat agcagctagc
     1441 cccttttcta tccaccctaa acgcagtact gaagtaaaag ctaagaatta tcatagataa
     1501 ttttagaatt gatttatgcc catttaacat aatgggcgtt atccgaaatc gtcttttata
     1561 agttaaataa aatcatatgc ttaactattt tacatcaccc gtaaccaccg caaaaatccg
     1621 ctttggaaac aaagcggatt ttttttgagc aagtttgata cttcttgcca ataattttga
     1681 ctaaaaatta ctccaattag cttgagtgat acaaatttgt gatacattat tgtatataca
     1741 aatttgaatg ttttttatgg aactgtattt cgaatgggat gaggctaaaa accaaaagaa
     1801 tcagaaaaaa catgatgtat cttttgaaac ggcaagcctc gtttttgaag atcctttacg
     1861 gatctcaatc caggatcgac ataccaatgg tgaagaacgt tggcaaacga tcggtatggt
     1921 taaaggtgta ctgatgttat tggtggctca tacaatcatt gacgatgatg actgtgagat
     1981 catacgaatc attagtgcaa ggcaagtcac taaagcggaa cgcaatcaat atgagcatgg
     2041 ttagatatac acgcaaagaa ctgaatgaaa aattcagtga caagcaagat gctgaaatta
     2101 aacgcttgct tgcaaaagga actgtaccag atgaacaatt agatctatct gacattcctg
     2161 agattaccga ctggagtcat gctgttcgcc atgggcaatt ttatcgtcca gtgaagcaac
     2221 aaacatctgt tcgtctagat gcagatgtac ttgcatggtt aaaggctcaa ggcaaaggct
     2281 atcagacacg catgaataaa attctgcgtg aagcaatgct agaagatctt aagaatcatt
     2341 gatgaaaatg ggagctatag actcccattt ttgcttcgca taagagattt tatgttaaat
     2401 aattaaattt tttctatgta aatctgacag tcttacgaag agtaagctag tagaaatctt
     2461 tcgatatctt cgatcatcct gtgaacatct tcttttgtaa actgatcata atctccatgg
     2521 gccgcattat ttcgaatatc agccatagct gtaattagtt tttgctgtag agggttataa
     2581 actcccgctt ttgccaagtc ttcattcaat cgtgttaatt ttttcttctc taattctatt
     2641 ccattattca aacatagttc tttaattgcg gtttctagaa ctacaccagc aattacagca
     2701 gctgaatttt tataaccact acttaacagc gatcgtgctt gttctaattc actatcaaaa
     2761 acttcagctt gaacaatttg cttgaaagaa gttaaaaaac catttttaaa atcatcatac
     2821 gatgctttta aaattggaat taacctactg attattctag tattagtatc aaagctctgt
     2881 gtttcttcag catctaaaaa atcacggtaa tgaatagaat gttcaccaca tgatagtaat
     2941 aatagatttt taactttaac tttccattca agataaagag catccccctc atcacccaaa
     3001 gtatcatagc taccgctata ttctgagtaa gctttttttg tcatagcttt gatggaagca
     3061 gaatcggtta gtagttgttc ataacgctgt aatatttttt gactcatgtt tactcagttt
     3121 tttagggttt ccaaattaag taattgaact ctataaatct atttagatga aaattggaaa
     3181 attaatgaaa acaataaact actgaaagat atccaaagat taggatcttt aaatttttct
     3241 ttaaggcctg aagtcattaa tttttgagat atttcttctt gattagttga ttccagaagg
     3301 ttttgaataa ctgatgccaa ttcataaggc tgaatagtat ctggaagttt tggggctaat
     3361 tgagtaagaa tgatttctaa tggtgattgt tgcggtgagc caatagtaat tccattccct
     3421 ttattgtttg agaaatagcc attagatatg gtaatacttc tgaaatcctc aggattattt
     3481 attcttagcc catcaccatc attataagat gagttgattc catcaagatt tagatgatcg
     3541 ttctgttttt tatcagtcat atataagcct ttatcttttg aatgatttat aacttatatc
     3601 atactgatca gaccaaatga aaaaagtgag cttaaactca cttttctata tctatacatt
     3661 gaatcctata tgtttcccag ttggaagttc cacatcaata cggagctttc ctcccatagc
     3721 ttcaacatac tttttcatgg ttgaaatctt aagatcattg ccacgatttt ctattgctga
     3781 aagcgatggc tgttttattc ccaaagtttc agccagttct ttttgagaga tttcgagttc
     3841 ttcacgaata aggtaaagct gattttctaa tagcaattca tcagccattt tttgaatacg
     3901 ggcttggctc tcaggagagc gattagccaa taattcttgt agggtcttag ccatcagatt
     3961 attctcctaa tgttgagaga tgaagttcat attgcttatc tgcaattgct agcatctctg
     4021 tataaaaacg ctttttaccg ctcttatcac caatacacag tacaattgct tgtcgtaatg
     4081 gatcgaatgc aaaaaatgct cttaatggtt tacctcgatg ctgaacacgt agttccttca
     4141 tattggtaaa tttagaatcg tagacggtat ctactaaggg acgacctaaa cttggtcctt
     4201 gcagttgcag aacaaccaac gcagctaaga ctttctcttg tgttatttca tcttgctgtt
     4261 caagccattc attaaacaga tccgttgtaa tgactgtcca catattacaa ccataatata
     4321 gattatagtc tatatattaa agctgattga ataactttgc aaatggcaat aaaaaagccc
     4381 gacatcctgt cgggcttttt catatcgggt gctcgtctta ctgatctcgt gctgcccatt
     4441 ccttgcgcta ttttcttatt cttgttgtct ggagacatcc agttccctta tgtattcata
     4501 atagggctag taccttggat taccaatcag caaaatcctt aaatctctgt aagctttagc
     4561 ttacaaagtt ggcctatttt atcccaagag ttgtccgcag aaattgggga ttatttgtga
     4621 agtgtgacca ttgagaaatc aggaaaaaat gaaaaacgaa tgttttttga tcgattttat
     4681 tggctaaaag ttacagtttt gcttaaaaag aaaccgactt gtttcccagt cggttttttg
     4741 tggtttttta gggggatgaa aaatttttaa ctatatgaaa aacaaggaga ttttaaaaag
     4801 catttcgtat aagctctatt atgttaaatg gaatgtttat gagaaattaa tagtctattt
     4861 ttgtttgaga ttgaagtata tttaaaaaag ttaagttgtt tttttaaatg cataacttca
     4921 tcttttagtt ttttgatttg ttgctctctt tcgtcctgta tagggtaagt aagttcacca
     4981 aattttctaa ctaactgact gtattcaatc aatttattgt agtcaagttc gaaatctccg
     5041 ctttttatat atttaagtat agtcgagcga ggttttttat acgctatagc agcatcactg
     5101 atcgaaaatt tttttaaaaa atgatgattt ttacctagca tactatttca cttaaggcat
     5161 tattaatttc gtatatatat gttaataaaa ttaacacata caagatataa atctttaatc
     5221 attattggtt aataatcgaa cgtttacttt acacatgccc tactctgata atttttctat
     5281 tattaaatca atatgctttt aaaaatttat attattaaca taatacacct tatacgaaat
     5341 gtgttataat tttccttaga aatcaacgct gtagtttctt ttttgcccaa cctccccaag
     5401 gtgggctttt tttttatgat ttttcacgtt ccacgcttat tatttaatca aagtttcacg
     5461 actttattca tattaattaa acaatcgtgg ttaatcatag ggtctaaaaa aacggctcca
     5521 aaagcctctt ttctcaggct ctggttcaat gtgctctggc actggaatcc acttattttc
     5581 cctccgctct ggagtagtca atccgtcatg tttagcttct gctgtgtcag gttcgggatc
     5641 tggtttggct atgctgggtt gcactaattc ttgatcggta aacgtagtca tattaggttt
     5701 tggggcttct aataggcgtt gcatcgtttc aatttggttc tgatagaaag attcccgatc
     5761 tttggcatca tcaagctggt ttttcagcat gtctatctgc tgtctaagta gtaaaacttc
     5821 tgccgaattt ggactgtcta tttttacagg ttcttttaca tcctgttttt tgacaggttc
     5881 cccgaaaaca cggatagcct cggctagatc gatcttattc tgggaatttt tacttaaaat
     5941 tcctttcttt atgttgttgt aaatcgtttg tctattgata ttgtaaagct tagaaagctc
     6001 gagaactgta aggcttttca tgttgtaaat ctatgcctaa acctgtcaaa agttgttgtc
     6061 tattagactg tctaataaac aaatgatcaa gtttaaattt taggcaggtg caaagccaac
     6121 tttttgaaga taagggaata attcttggaa tttttgataa tcttggagca tttctgcaat
     6181 gcgtatagca aattgttgga agctttcggt gccttctgaa tattgcccca tctcaggtaa
     6241 ttcagaaagt ttgttggcaa agagatgacg ttgtttatca ctcattttgg tgaaaagggc
     6301 taatgtatcc gttcccttaa ttactttgtc agagttggtt tttttctgct taaaactgaa
     6361 agaaaaaccg gaaattgaac gtcctttttt atgttgttca tatttagccg ttatatcggt
     6421 taactcattt acttgcttga gggcaaaatc taaaacatac gttttaaaat tactcattgt
     6481 tttgtattga tgagcttcga caccaagttg ttgtctaaag atcgatagat caaaaattgg
     6541 cgttttacca gcagatctcc attgaattaa taactcgtat aaccgaatag cataagagct
     6601 agttaatctc gaaacctgct ccaattcata tttagtaaag ttttcttcca gtcgagttat
     6661 gagtggaaca atgtcttgcg tgaaacgtag gaaaacgatc ccggattgag gttcatatcc
     6721 aattttatcg acccaacgac agtgaaagct acgatcctta ccagtcttag gattaatgtc
     6781 atgatacgtg acatagcgat caaacaagct tttagatgca tcccgaagca cggtataagc
     6841 agtgtgtttc tcgacattaa aggtattgat ataactactt gcatgtactt ctaaaaggct
     6901 attttcagtt atcccgtgcc ctgtttcccg agcttcagca atggctaata ggatcagcct
     6961 ttgttcaact gtatctaggg tatagctagc ttgaattaaa gcattatcct ttacgattaa
     7021 ttcactcatt tctaataata aatttataat taaactaggt tgtacaatag actattttaa
     7081 ttgaattgta aatcgactta atttggggtc gataaaccta gttttaaggt cgataaacct
     7141 agttttatgg gtcgataaac ctagttttat gggtcgataa acctagtttt atgggtcgat
     7201 aaacctagtt ttattaactc taaaactatt atgaataaag ggttacagct tacttaaaag
     7261 catttaaaag attaaaaata attaaaagcc caggtaaaga aaaattgccc ttggttttcg
     7321 cttcgctcaa actctattga acttcgcttt cgctcagttc gtcggggcaa ttttttggtg
     7381 agcacttacg tgactttaaa aaagcaaaag caactcggaa ttcgctacgc tcataaaact
     7441 tttttactcg ctacgctcga tctagactca aatttctaca acttttccat gcttatttag
     7501 gtttcacagc ctaaaaagcg gtttttcagg gagtctaggc aaaattttcc aaggatcgct
     7561 cgtagacact cgctttgttc ttatcggtaa aatcaaatga tctccaaaat actctaattt
     7621 gcgtttagag cactctaatt tttttattga tggggaatat aggtttaggc taaaaaaggc
     7681 atatacggct tcaacagtgt atttaaaccc tattatttga gtcaatctaa gctttattga
     7741 aaggattcct tggatcttct ttggcttgct taatcaattt ctccatttca tatagcgatt
     7801 tagccgataa aatatctgtt tttctatttt gatccaggct aaatgcattg ttcttttctg
     7861 ctccggattg tgcctgctta aataaatcga gcattcgatc taaatcttcg tttgaatttg
     7921 atcgctctaa accgctttgt tccaataagt ttaaatatag tgatccgata aaatatttaa
     7981 tgtggtcttg ctgttttctt aaagatttac taaggggggc ctggttatat ttctcagggt
     8041 ttaaaactcg ttcgaccttc gctgtaatat tcttttcaga agtctgattt cttttaattt
     8101 ttaaaacttc atttaatgca tcatgcattg caaagtaagc atcaatatct gtttttttac
     8161 ttaattctag aatattcaat tctaattttt ttaatttctg tggctcggtt ttttggatca
     8221 gttgtaattg aactatacgt ttattataag tattgcgctc ataacgacat cgttgaataa
     8281 gttttaaatc ttcttcattc agttgtttca ttgattctcg ttcagaaatc attgctaaaa
     8341 aagcatcttg cattcgtctt ttaaacggca gcgctcgaat atgtttatcc aaatcgatga
     8401 tttcttgttg tgttaaggca aatgtttttc taccggccaa agcggatttc ctgattttgg
     8461 gtatctaaat tgatgttagc ggaattctga tggtgttgaa acaatatttt atgttataca
     8521 taaaatattt tttatctcgt ttccgtgtct ttttttaaca atgattgctt tagcaatcat
     8581 tgttaaaaaa agacacttct actcgctcct gcggaggcta cataaaccac tgatttaatt
     8641 attaaattat gagtttttct tcgggtttta acggtatttt tcttaatggt gaaaaagtaa
     8701 gcaatgtctg ccgttacaag aaatgggaaa ggggtgcaac aaggaaaaca gcgaatttta
     8761 gacgagtgca acaaactttt ttgatgaaat atcgatgttt gcaacaagaa gagactgctc
     8821 tctgcaacaa gaattagccc cttgttacaa gaaaatttgc tgtttcgtta cacgtaattt
     8881 gtttgaggtg aactgatgtt ttatttaaat ctaaagcata atattaaaaa agagcagagt
     8941 tctctgaatg cattccatta tttaactcgg acggcacatt ttgaaaatca aaaagacaat
     9001 gaaaaattag agtttatgcg gtatggaaat atgcccaaat gggcagaaaa taaaccaaaa
     9061 cttttttgga aaagtgctga tcaatttgaa atttcccggg gaagaacaag ctctacttta
     9121 accattgctt taccaaaaga gttaatacta gaacaacgag cagaattggt tcagaagtta
     9181 atagatcagt ttgccggaca atatcaattt ccttataccg cagtcattca taatcatccc
     9241 agtgaaatta ctggagagga tcaaccacat ttacatttga tgtattcaga aagaacaatt
     9301 tcagatgata ttgaacgacc accagagcag ttttttaaac agtaccggcc aaaaaatcca
     9361 actcaaggtg gtgcgcaaaa gttaacagct gatgcattag gttttgggcg aaatcaaatc
     9421 aaagtattcc gtgaaaaaac tcaagaatta atgaatttgt ttttagaaaa atatgcacca
     9481 actaaaaaag taatggttta tggtgtagaa attgaagtga aaaatcaggt cagttgccta
     9541 tctaatgacg atttcaatca aaaattcggg accaaattac aggatgtacc acaaatccct
     9601 cgttggaaac tttatagcgc tgatccaatt attcagcttg atgttgaagc tcaaaaaaac
     9661 acaattaaaa aaattagaaa tcagaacaat gctgaacttt atggcaagga atatgattta
     9721 gaaatatcta gacgccatgt gcttactcag aaaaaagatc ctggctttag cttagattga
     9781 tttttataag tggaaaattt gtgttttttg aacatggaat cttgaaaaaa cttatccata
     9841 ttgtgagtga tttttagcca caacatggaa aatttgtgtt ttttaggctg caatgatgga
     9901 tagattgtgt ttttgattca ggtcaataag gaaaacctag atttttgccc ataaatgtaa
     9961 aatctaggtt tttcgcataa ggtgtattat gttaatttta gaaaatctaa aaaagggata
    10021 gttaaaaact atccttttat cttttaaaaa ttatcttaat aataatactg gttttgtaga
    10081 gcttgataac aatttggtcg tcgtactacc aataaaaaac tggcgtatct ttgaatgacc
    10141 atatgcacca attacaatca tatcaataac gtttttttct tgataatcca atatagattt
    10201 ctcaacatca ccgtcaagca tgctatcaat tacatcaaaa ccattatttg caagtagctc
    10261 ttttgcccaa gtgacttgta atgtcatatc actatttggc ttaccaacat ataccacatg
    10321 agctgtcagg gtttttaata aagggctatt tgcaattttg tctacacaaa tacgtgcagt
    10381 aggacttcca tcaaaagcaa ttaaaaaaga cttaggtgga ctaaagggtg aagatgttat
    10441 taatacgggt ttatgaagtg cacgaacaac attttcaata tgggttccaa ctttactgga
    10501 atcatgctcg gtttcagccc catgtttacc cataacaaga acacgaatat cctcttccat
    10561 tcccatgatt gtctctaaaa gactgccgtg gcgttgaaaa ctttgagcat caatagagaa
    10621 cttctccaat agataattct ttgcttcccg aagaagaatt tggccatgct ctagttcaat
    10681 cttcccctta cgctcttcaa tttcaaccaa ttctttgagc aaggtttctc gacttcccaa
    10741 gccgattgca ccgcttaaat cttgtggtgc tttcgctttt ggcttatcaa taacatgtaa
    10801 taacttaagc ggtgaactaa gtttatcgct aaaccaagct gcataatcac aaacggtatt
    10861 tgttgctaat gatccatcaa tacatgcaat aatttttgac atttttcatc cctcttagtg
    10921 gctatttaag cgatctaatg catctggttt atcatggatg ccaaatttat cgactaaggt
    10981 tttgctggct tcattcattc caataagttc gacttctgtt ccttcacgtc taaacttaat
    11041 gaccacttta tctacggcag cgaccgcaga aacatcccaa aagtgagcct gactaacatc
    11101 aattacaact tttgaaagtg cttctttgaa atcaaatgca gaagtaaatt tatctgct
//
