LOCUS       NZ_CP069493             5219 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1264 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP069493
VERSION     NZ_CP069493.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357406
            Assembly: GCF_016889265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 990.89x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 04:51:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,921
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,621
            CDSs (with protein)               :: 4,621
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 73 of 187
            Pseudo Genes (incomplete)         :: 130 of 187
            Pseudo Genes (internal stop)      :: 34 of 187
            Pseudo Genes (multiple problems)  :: 45 of 187
            Pseudo Genes (short protein)      :: 3 of 187
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069493.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1264"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1264"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            36..566
                     /locus_tag="I6J94_RS24315"
                     /old_locus_tag="I6J94_24320"
     CDS             36..566
                     /locus_tag="I6J94_RS24315"
                     /old_locus_tag="I6J94_24320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_011666801.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFRKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            573..806
                     /locus_tag="I6J94_RS24320"
                     /old_locus_tag="I6J94_24325"
     CDS             573..806
                     /locus_tag="I6J94_RS24320"
                     /old_locus_tag="I6J94_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1351..1647)
                     /locus_tag="I6J94_RS24325"
                     /old_locus_tag="I6J94_24330"
     CDS             complement(1351..1647)
                     /locus_tag="I6J94_RS24325"
                     /old_locus_tag="I6J94_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047647.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLTQ
                     ADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
                     /name="hypothetical protein"
     gene            1859..2056
                     /locus_tag="I6J94_RS24330"
                     /old_locus_tag="I6J94_24335"
     CDS             1859..2056
                     /locus_tag="I6J94_RS24330"
                     /old_locus_tag="I6J94_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001335786.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSVS
                     GLDVELIDVSCESEWEEHSH"
                     /name="hypothetical protein"
     gene            2074..2358
                     /locus_tag="I6J94_RS24335"
                     /old_locus_tag="I6J94_24340"
     CDS             2074..2358
                     /locus_tag="I6J94_RS24335"
                     /old_locus_tag="I6J94_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="WP_000625497.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="colicin Z C-terminal domain-related protein"
     gene            complement(2422..2811)
                     /locus_tag="I6J94_RS24340"
                     /old_locus_tag="I6J94_24345"
     CDS             complement(2422..2811)
                     /locus_tag="I6J94_RS24340"
                     /old_locus_tag="I6J94_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000477210.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNPTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            complement(3998..4189)
                     /locus_tag="I6J94_RS24345"
                     /old_locus_tag="I6J94_24350"
     CDS             complement(3998..4189)
                     /locus_tag="I6J94_RS24345"
                     /old_locus_tag="I6J94_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4254..4577
                     /locus_tag="I6J94_RS24350"
                     /old_locus_tag="I6J94_24355"
     CDS             4254..4577
                     /locus_tag="I6J94_RS24350"
                     /old_locus_tag="I6J94_24355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_000955995.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="plasmid mobilization relaxosome protein MobC"
     Typing          3826..3912
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          complement(3259..3815)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.821"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..910
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.67"
                     /coverage="99.0"
                     /evalue="4.8e-175"
     Typing          4568..5217
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.539"
                     /coverage="99.0"
                     /evalue="8.199999999999999e-140"
ORIGIN
        1 cggcgggaag aatatcagac tgaaaggagc attctatgag caatctttta cagacgggcg
       61 cggagttcga gaaaaagctg aaagagagag ccgaatctac agagacaatg ctgaacgacg
      121 agttcaggaa gctcgaaaaa tctgtaagca aggctgtgac atcaaacgag acgaaaatca
      181 aagacgctat agccctgttc acagcttcga cagaggaatc actgaaaaaa caccgggaag
      241 gggtgaaaga ggcgatgatg cagcacagga aggacgtgtt aaagctggca gggaatacgg
      301 gcatgatgtt actggggatg gtcattttcc tgtttaccgt gagtggcggg acgctctggt
      361 atcttggcgg gatgatacag gcgaacctgg aagaaatcag gaagcagaac gagaatctgg
      421 agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggggagcaac ggacgttttc
      481 tggtgttgcc cgaggggatg aaagccgaaa cgaactggac gatggaaaac gggaaacaga
      541 atgccgtaag gctggtacag gagtaacaga gcatgacgga gctggaaaga catttgctga
      601 acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg gagagcgcct
      661 tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc
      721 gggtcatgca gttgagtcag caggtgcagc acttgagcga gcagacagaa cgcttgagcc
      781 agttatacag cgagaacagg agataaggga cgaacggctg atacaggaac gcgaacatgc
      841 gttatccctg gagcgtgagc gccagctgga aatacaggaa cggacactgg atggcccttc
      901 gctgggatgg taatcagaga caggctaaaa tgttgtcagc gcgtttccgg tgcatttttt
      961 gggataatgg ttggttcggg ggcaccggga acgcgcttca gtcagacctt gctgaaaaca
     1021 caggaacgcg ttaaaacgca tacgggcgcg attctggtgt gtcttttgtt gctgtgtttt
     1081 gcctctattg ccagatcgcg ctcattcccc tgaacttctg ttttcccgct ccggtccccg
     1141 tcttcttcgc ttccgaaagc atgaaaaaaa tccgtttcag ctgtctctgg ctggcaattc
     1201 ctgcatgatt cacatggctg catagctatg catgtggtta aaatttaccg ggtgcgatcg
     1261 cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgccga
     1321 acgcgtttca gcggtgcgta caataagtga ttatggtaaa tcacccttcc aggcgttcag
     1381 cccttcttcc ggtttttttc cgttcaggaa tttacgggcg agacgaatgc gtcttgcctg
     1441 gtaaatcgca tcgtaggcct gtgactgtcc cacaccgtta ctgatttcac tgaccagatg
     1501 gcgcaggtcg gcctgtgtca gtgtgtaaac gtgtttctgg atgaggtcac gggctgtccg
     1561 ctcttcgtat ccggcagcga taagtgcagc cagcaggcgt ttgtctgttt tgctcagtcg
     1621 gtggcgacca gattttgttt tgctcacgtt atctcctctc tgttttttct cctgatgtta
     1681 tccggtgctg tttttctgct gcgaatcttc ccttcctgat ttttataagg gtgattcaga
     1741 tcacaaaaat gaaaattcat atcattgaca ggaaaaataa tgcggcgtaa tttatactgt
     1801 atataaaact agtatttata tatacagtat attctgtgta aataaaggga gggataaaat
     1861 gaaagaagaa aaagtattgc ttcaccgttt cttatttgta gttaggaata agaatggttg
     1921 tgaactatca tgttcggcag accttatggg aacaagggat gatgtttata agtatttctc
     1981 tgattctgtt agtgggctag atgttgagct tatagatgtt tcttgcgaga gtgaatggga
     2041 agaacattcg cattgaacta atcaggagat tatatgattt ctcaaaatgt ttgggctcct
     2101 cctttctttt ttggtccttg ggttgaactt gaaaatcata ctggtgatat cgcgtacact
     2161 gtctattttg atacattgtc taatgtaccc ggtccattta ttgcttttat ctggtatgcc
     2221 cctaatgggc caagaacagt taacgttatt ggtccagcat cttatcagat ctcttgcaag
     2281 ggcgctggtg ttgataaaat taaatttaaa agtgtcagtg ggacagggca gattattcat
     2341 gtatacacta ccttataata agagctgaat aaatataatc ctgcctaagc tataccttaa
     2401 gcaggattat atctattaat attattttga atatgctttt atgatttcca ttactgttgt
     2461 ttcatcgttc acaggttgat aataatgggt tggatttagt gcagaatgaa tgattgcagg
     2521 ttgggatatt acttttccat caaaatatga aatcaggggt ttccctatat ttttactagt
     2581 tatttcgttt agttttttct tacataaact atctgtgttt acttctatta tgacgccttt
     2641 gtttttttct gattgggaag ggtacatttt aagtatgcaa tatttattca gaatgatgct
     2701 gtcaccacca actttgtaaa caatgttttt gtttgtttca gtacttgaac atcctataag
     2761 taaggtcagt atagtactcg atgttattga ctttactgat gatttgaaca tattaactcc
     2821 attttatttt gttaaaattc caatctataa atacatatac acgtaaaaat aaacaatgcg
     2881 aacgttttca cttatattat ccccatgatg tgattgaatg gtggttccat ataagagagg
     2941 gcaattatac tatctttctc tcctgaactg atgggattgc agtttagaga aaaaagatca
     3001 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa acgaaaaaac
     3061 caccctggca ggtggttttt cgaaggttag ctaatcctgg cagattacct aaccgggtag
     3121 ctggcttaag tggagcacag ataccaaaag atgtcctttc agtgtagccg ctataaggct
     3181 tcaacttcaa gagttctgcg tatctaaatc cactttttag ttaccaatgg ctgctgccag
     3241 tggcgttaag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3301 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3361 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3421 ggcggacagg tatccggtaa acggtaaggt cggaacagga gagcgcacga gggagcttcc
     3481 ggggggaaac gcctggtatc tttaagtcct gtcgggtttc gccacctctg acttgagcgt
     3541 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag cggcgcggcc
     3601 ttcttcctgt tcctggtctt ttgctcacat gttttttccg gtgttatccc ctgattctgt
     3661 ggataacagc atttcgtttt tgagtgagta gataccgctc gccgcagtcg aacgaccgag
     3721 cgtagcgagt cagtgagcga ggaagcggaa aagcgcctgg acgtgcattt tctccttacg
     3781 catctgtgcg gcatttcaca cccggtatgg cgcacgtttc atacaatccg cactgatgcc
     3841 gcatggttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     3901 gacacccgct aaaacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctc
     3961 acagacaagc tgtgaccgtc tccgggagct gtatgtgtca gaggttttca ccgtcatccc
     4021 cgaagcgtgc gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc gattcacaga
     4081 tgtctgcctg ttcatccgcg tccagttcgt tgagcttttc cagcagcgtt aatgtctggc
     4141 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttagtcact gatgcctccg
     4201 tgtaaggggg gatttctgtt catggggtaa tgataccgat gaaacgcgag aggatgctca
     4261 cgatacgggt tactgatgat gaacatgccc ggttactgga acgctgtgag ggtaaacagc
     4321 tggcggtatg gatgcggcgg gtctgtctgg gtgagccggt tgcccgttcc gggaagctgc
     4381 cgacactggc accgccgtta ctgcgccagc tggctgccat cgggaataac ctgaatcaga
     4441 cggcgaggaa ggtgaacagc gggcagtggt cttccggtga ccgggttcag gtggtggccg
     4501 cactgatggc catcgagaga gagttgcgca gcttacggca ggtcgtcagg gaacatggag
     4561 cgcgggatga tagttaagtt tcatgccagg ggaaaaggcg gtggcagtgg tccggttgat
     4621 tacctgctgg gaagggagcg taaccgggaa ggggcaacgg tgcttcaggg taatccggaa
     4681 gaagtccggg aactcatcga tgccacgcca tttgcgaaga aatacacgtc cggtgttctg
     4741 tcgttcgcgg agaaggagct gccgccggga ggacgtgaaa aagtgatggc gagctttgag
     4801 cgtgtactga tgcccggtct cgaaaaaaat cagtacagca tcctgtgggt ggagcaccag
     4861 gacaagggac ggcttgagct gaattttgtc attccgaaca tggagctaca gagcggaaaa
     4921 cgcctccagc cgtactacga ccgggcagac aggccgagaa ttgatgcctg gcagacgctg
     4981 gttaatcacc attacgggct gcatgacccc aacgccccgg agaaccgccg gatactgact
     5041 ctccctgata acctgccgga aacgaaacag gcgcttgcag agagcgtcac acgcgggata
     5101 gacgctcttt atcatgtcgg ggagataaaa ggccgtcagg atgtgattca ggcgctcaca
     5161 gaggcagggc ttgaagtggt cagggtgacg cgaagcagtc tcagcattgc agacccgaa
//
