LOCUS       NZ_CP069491             9318 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1264 plasmid unnamed3.
ACCESSION   NZ_CP069491
VERSION     NZ_CP069491.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357406
            Assembly: GCF_016889265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 990.89x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 04:51:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,921
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,621
            CDSs (with protein)               :: 4,621
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 73 of 187
            Pseudo Genes (incomplete)         :: 130 of 187
            Pseudo Genes (internal stop)      :: 34 of 187
            Pseudo Genes (multiple problems)  :: 45 of 187
            Pseudo Genes (short protein)      :: 3 of 187
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069491.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1264"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1264"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            157..372
                     /gene="ccdA"
                     /locus_tag="I6J94_RS24210"
                     /old_locus_tag="I6J94_24215"
                     /pseudo=""
     CDS             157..372
                     /gene="ccdA"
                     /locus_tag="I6J94_RS24210"
                     /old_locus_tag="I6J94_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /name="ccdA"
     gene            374..678
                     /gene="ccdB"
                     /locus_tag="I6J94_RS24215"
                     /old_locus_tag="I6J94_24220"
                     /pseudo=""
     CDS             374..678
                     /gene="ccdB"
                     /locus_tag="I6J94_RS24215"
                     /old_locus_tag="I6J94_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /name="ccdB"
     gene            679..1488
                     /locus_tag="I6J94_RS24220"
                     /old_locus_tag="I6J94_24225"
     CDS             679..1488
                     /locus_tag="I6J94_RS24220"
                     /old_locus_tag="I6J94_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237314.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_000016979.1"
                     /translation="MSGSVIHSQSAARVPAVYSAGQSPQLPVVIDYPAALALRQMSMVH
                     DELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQ
                     LATLKQRTEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPLERRNKRTGR
                     TEKARIWEVTDRTVRTWIGEAVAAAATDGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVL
                     QSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNRHA"
                     /name="site-specific integrase"
     gene            complement(1626..1901)
                     /locus_tag="I6J94_RS24225"
                     /old_locus_tag="I6J94_24230"
     CDS             complement(1626..1901)
                     /locus_tag="I6J94_RS24225"
                     /old_locus_tag="I6J94_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000239529.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQCR
                     TTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
                     /name="hypothetical protein"
     gene            complement(1895..2539)
                     /locus_tag="I6J94_RS24230"
                     /old_locus_tag="I6J94_24235"
     CDS             complement(1895..2539)
                     /locus_tag="I6J94_RS24230"
                     /old_locus_tag="I6J94_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000633911.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAAK
                     HHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAHQI
                     IAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLEYLE
                     EFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAAKAEIEHLMNEVFGPW"
                     /name="AAA family ATPase"
     gene            2768..3739
                     /gene="parM"
                     /locus_tag="I6J94_RS24235"
                     /old_locus_tag="I6J94_24240"
     CDS             2768..3739
                     /gene="parM"
                     /locus_tag="I6J94_RS24235"
                     /old_locus_tag="I6J94_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="WP_001103694.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQT
                     FNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTLPI
                     SEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVVDKVG
                     QFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSDTSFL
                     VADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVNRVYVV
                     GGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
                     /name="parM"
     gene            3744..4136
                     /locus_tag="I6J94_RS24240"
                     /old_locus_tag="I6J94_24245"
     CDS             3744..4136
                     /locus_tag="I6J94_RS24240"
                     /old_locus_tag="I6J94_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="WP_000340835.1"
                     /translation="MDDERKRKKYTLYLHPEKAADFQTLEAIESVPRSERGELFRNAFI
                     SGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRTVLTELGASQSV
                     EKMPPSATDSVQEAMNDVRLKMKKLF"
                     /name="plasmid partitioning/stability family protein"
     gene            complement(4141..5412)
                     /gene="umuC"
                     /locus_tag="I6J94_RS24245"
                     /old_locus_tag="I6J94_24250"
     CDS             complement(4141..5412)
                     /gene="umuC"
                     /locus_tag="I6J94_RS24245"
                     /old_locus_tag="I6J94_24250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /GO_function="GO:0003684 - damaged DNA binding [Evidence
                     IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="WP_182574825.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEAK
                     ALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDEMF
                     IDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFSGVV
                     ALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKNFSVI
                     LERTVRELNGESSISLEEVPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAAEKLRG
                     ERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLSLPTQDSRDIIAAACRVLNHVWREGYR
                     YMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFAGRGTAPE
                     WQMKREMLSPSYTTQWTEIPIASF"
                     /name="umuC"
     gene            complement(5412..5849)
                     /gene="umuD"
                     /locus_tag="I6J94_RS24250"
                     /old_locus_tag="I6J94_24255"
     CDS             complement(5412..5849)
                     /gene="umuD"
                     /locus_tag="I6J94_RS24250"
                     /old_locus_tag="I6J94_24255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="WP_000109079.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDLN
                     SYCISRPAATFFLRTSGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKRLL
                     LRPRPALEPVSDSPEFRTLYPENICIFGIVTHVIHRTRELR"
                     /name="umuD"
     gene            complement(5846..6094)
                     /locus_tag="I6J94_RS24255"
                     /old_locus_tag="I6J94_24260"
     CDS             complement(5846..6094)
                     /locus_tag="I6J94_RS24255"
                     /old_locus_tag="I6J94_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="WP_000619112.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRIDQ
                     GSAPSVSVTGARNDKGKERIQSLLEEIWQDDSWLPAA"
                     /name="DinI-like family protein"
     gene            6229..6747
                     /locus_tag="I6J94_RS24260"
                     /old_locus_tag="I6J94_24265"
     CDS             6229..6747
                     /locus_tag="I6J94_RS24260"
                     /old_locus_tag="I6J94_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="WP_000115885.1"
                     /translation="MSVPQTKAELLLAIDKNFSKLISYLNTIPPEITSDKSMDGHAKGT
                     EMSVRDLVSYLLGWNALVVKWIASDAKGLPVDFPETGYKWNQLGLLAQKFYSDYSELSY
                     ELLVAELQTVKNEIVNLINDRTDDILYGRPWYTKWTMGRMISFNTSSPYANANGRLRKW
                     AKNNNISLK"
                     /name="ClbS/DfsB family four-helix bundle protein"
     gene            6766..7986
                     /locus_tag="I6J94_RS24265"
                     /old_locus_tag="I6J94_24270"
                     /pseudo=""
     CDS             6766..7986
                     /locus_tag="I6J94_RS24265"
                     /old_locus_tag="I6J94_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc53 family transposase"
                     /name="ISL3-like element ISEc53 family transposase"
     gene            8405..9235
                     /locus_tag="I6J94_RS24270"
                     /old_locus_tag="I6J94_24275"
     CDS             8405..9235
                     /locus_tag="I6J94_RS24270"
                     /old_locus_tag="I6J94_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="WP_001298676.1"
                     /translation="MYFSGEPAKIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLLQ
                     TTEDVRFEPCPGLTAAGRGVLSPENITFTRWLKHLQDGVLLDEQNCLMLHELWLQSGTG
                     QRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLSEKTMPFDLLTV
                     LPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDFDTPW
                     CQPESDVVAELSRRFGCTLEHWYAEQGCNFCGCVNATMFDRYLPFSCPLRYFRY"
                     /name="DUF1281 domain-containing protein"
     gene            complement(9229..>9318)
                     /locus_tag="I6J94_RS24275"
                     /old_locus_tag="I6J94_24280"
                     /pseudo=""
     CDS             complement(9229..>9318)
                     /locus_tag="I6J94_RS24275"
                     /old_locus_tag="I6J94_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /name="AAA family ATPase"
ORIGIN
        1 agccagattg atttttattg gttaacataa cgaaaggtaa aataaataag gcttactaaa
       61 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggcataagaa tatatactga
      121 tatgtatacc cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca
      181 gttgacagcg acagctatca ttgctcaagg catatgatgt caatatctcg gtctggtaag
      241 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
      301 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg cgatgagaac
      361 agggactggt gaaatgcagt ttaaggttta caccataaaa gagagagccg ttatcgtctg
      421 tttgtggatg ttcagagtga cattattgac acgcccgggc gacggatggt gatccccctg
      481 gccagtgcac gtctgctgtc agacaaagtc tcccgtgagc tttacccggt ggtgcatgtc
      541 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
      601 ggggaagaag tggctgatct cagtcaccgc gaaaatgaca tcaaaaacgc cattaacctg
      661 atgttctggg gaatataaat gtcaggctcc gttatacaca gccagtctgc agccagggta
      721 ccggcagttt attctgccgg acagtctcca caacttcctg ttgtcattga ttatccggca
      781 gctctggcac ttcgccagat gtcgatggtt catgatgaac tgccgaaata tctgctggct
      841 ccggaagtga gtgccctgct ccattacgtc ccggatctgc gccgcaagat gctgctggcc
      901 acactgtgga acaccggtgc gcgcattaat gaagcactgg cgctgacgcg gggggatttt
      961 tcgctcgcgc ctccgtatcc gtttgtgcag ctggccactc tgaagcagcg gaccgaaaaa
     1021 gccgccagga cggcaggaag aacgcctgcc ggacagcaga ctcaccggct ggttccgctc
     1081 tccgactcct ggtacgtcag tcagctgcag acgatggtgg caacactgaa aattcctctg
     1141 gaacggcgta ataaacgaac aggcaggaca gagaaagcgc ggatctggga agtgacggac
     1201 agaacggtca ggacctggat tggggaggcg gttgccgccg ctgctactga cggtgtgacg
     1261 ttctctgtcc cggtcacgcc acatacgttc cgccattcct atgcgatgca catgctgtat
     1321 gccggtatac cactgaaggt cctgcagagc ctgatggggc ataagtccat cagctcaacg
     1381 gaggtctaca cgaaggtgtt tgcgctggat gtggctgcac ggcaccgggt gcagttttcg
     1441 atgcctgagt ccgatgcggt cacaatgctg aaaaacagac acgcataatt cataaatcat
     1501 aattatgaat tgtgatttat tctgtaaaaa aagagaccac tgcaatatgt gatctcttgt
     1561 atgcaagggt gcttaaacag tatgaattca caagtgtgat ttcataagta ataacttatt
     1621 gattattacg ttagtgtttt taagtattgc tcaagggcta ctctgacgat ggcacttaca
     1681 ttttttggat ctttaccatt gcgtttattc ctcaatgcca gatcttctat tgtgacaagc
     1741 atgctttcac caagagagat tgtcgtccga cattgttttt ctggttcggg tttttccggc
     1801 gcgccgtatg gtttatccgc aaggcgctga gccagagcct ctgcttgttc taccgtaact
     1861 gcagctctgt ttaatgcttg ctggctgggt ttcttcacca tgggccaaat acctcattca
     1921 ttaaatgttc aatttctgct ttggctgctg tattgttcgt ttctactaca ccggtgccgt
     1981 tgctcatgca atcgcgatag acttttctga aacaaataac tgaatcaagg acttgaattg
     2041 ttgggaattc ttcaagatat tcaaggaatt cttttctctc atttcccctc aaaaccggat
     2101 tggttgttgc catactttgt aaacaataaa ccttaagttc cgggttaagg ttacgcattg
     2161 cgtctatctg ttgttctagt tctgtaagag tatccagatc tggttgagaa cactgtagtg
     2221 gcgcaattat ttgatgtgcg acaactccac tcgttatgaa ttcctttgag tttcttcctg
     2281 cgacatccac tattacatca tcgaactttt cattcagagc ctgtagtgtt tgtgtcaggt
     2341 tatcaaattt ctcaacaagg gttattgctg gtaacagtcc tgcagcttct cgttccgcat
     2401 gatgttttgc ggctgttcgt tgtagatctc cgtttaacag gcagacttcc cttcctttat
     2461 ttgcgagagc aatagccagg tttgatgcaa tggtagtttt ccctgaacca cctttgttcc
     2521 caccaactac agtaatcatt gcaacctcat aaatgtgatg tgtgaagtat gatgatattt
     2581 tgacacggta acctgaggtg tcaatatgtg ttcatattta tgaagtatga aatgtgaagt
     2641 gtgccatgtg atgtaataaa taatacttgt gttttgtgat ttcataacta tgatggtgct
     2701 caaattgagt attaccttgc aaccagaaac aatgatttac tcagattgag tatttaaggg
     2761 gctatagatg aacgtatact gcgatgatgg ttcaacaaca atcaagttgg catggaatga
     2821 taatgggaaa atctgtaagt cgttgtcgca aaactcattt cgtcatggat ggaaggttga
     2881 cgggttagga attcgccaga catttaacta tgaactggat ggtaaaaaat acacgtatga
     2941 tgaagttagc aaccaatcta ttcttacgac tcatattgag tatcaatata cggatgttaa
     3001 cctgttagct gtgcatcacg ctttactgaa tagtggatta gcgcctcagc ctgtatcatt
     3061 gactgtgact ttgcctatca gtgagttcta tacgaaagag tgtcaaaaaa atgaactcaa
     3121 cattcagcgt aaaattgaga atttaatgag gccgattcgt cttaataaag gcgatgtttt
     3181 caccattgaa cacgttgatg tgatgcctga atcattgcca gctgtgtttt ctcgtctggt
     3241 cgtggataaa gttggtcagt tcgaaaaatc gctggtagtt gatattggcg gcactacgct
     3301 tgatgttggt gttatcgttg gtcaatttga ctctgtaagt gccattcatg gtaattctgg
     3361 cattggtgtg tcttcagtta cgaaagctgc aatgagtgca ttgcgcatgg catccagtga
     3421 taccagtttc ctcgttgcag atgaattgat taaacgtcgt aatgacccgg actttgttcg
     3481 gcaggttata aatgacgaaa caaaaactga tctggtcctt aataccattg agggggctat
     3541 tgccagtttg ggggagcaag tagtcaatga gctcggtgat ttccatcatg tgaatcgtgt
     3601 ttatgttgtc ggtggtgggg ccccgttgat ctatgattcg ataaaaacgg catggcacca
     3661 tcttgggcaa aaagtagtga tgatggagtc gccacagaca gcactggttg aagctatcgc
     3721 ggctttcaaa gaggagtaat attgtggacg atgaacggaa aaggaaaaaa tacacacttt
     3781 atctgcatcc ggaaaaagct gcagactttc aaactttgga agcgatagag tccgttccgc
     3841 gttctgagcg tggtgagctg ttccggaatg catttatttc tggaatggcg ttacaccaac
     3901 ttgatcctcg cctgcctgta ttgctgacag ccattttgag cgaggagttt tcagcagatc
     3961 aggttgtaac cttactcagt cagacgactg gatggaagcc ttctcaggcc gatatcagaa
     4021 ctgtgttgac tgaactcggt gcttcgcaat ctgttgaaaa aatgcctcct tctgctactg
     4081 attcggtgca ggaggcaatg aatgatgtgc gtcttaaaat gaaaaaatta ttctgatata
     4141 tcagaatgaa gcaatgggga tctcagtcca ttgtgttgta tacgaaggcg atagcatttc
     4201 ccgtttcatt tgccattcag gggcggttcc tcgccccgca aaccacactt tcccttttcc
     4261 cgactggttc agttcatcga gtgttttcat taacttttca ctgtttttac ggggctggat
     4321 ttcatcaaat aatcccggct gcgctatacc cgatggtgtg aaatcagcca gcatgactcc
     4381 tgccttcata tagcggtacc cttcacgcca gacatggttt aagactctgc atgcggcggc
     4441 aataatgtcc cggctgtcct gtgtgggcaa tgaaagcttt tccacagcgg cattgctgta
     4501 acagggttct tttactgcaa agggggatgt ccgtacaaat gtcgtcacct gccgacaata
     4561 ctgacgctcc ccacgtagtt tctctgcggc ccgctctgca tactgaacaa cagcctggtg
     4621 catggcatct ttgtctgtga ttcgttcacc aaaactgcga ctacagacaa tctgctgttt
     4681 tgccggtggt acttcttcca gggatatgct ggactcgccg ttgagttcgc gtaccgtacg
     4741 ctcaagaatg acgctgaagt ttttccggat gaatgccgtg ttagcctgcg ccagttgcag
     4801 tgctgtgtta atacccagcg cattcagctt ttccgtcagt ctgcgtccta ctccccagac
     4861 ctcaccaact ggctgcagcc ccagtagctt caagatccga ttacggtttt ctgccgtcag
     4921 cgcgaccaca ccggaaaact gtggccattg ctttgttgcc cactgtgcac ttttagccag
     4981 cgtttttgta ggcgcaatgc ccacccccat ggtgagtcct gtccagctct ttacctgttc
     5041 cctgagctga tgaccaaaaa actccggaga gatgcaatga tttatccccc gcaaatcaat
     5101 gaacatttca tcaattgagt agggctcaac tgcgggagaa agcgactcca gaacagccat
     5161 aacccgttgg ctcatgctgt ggtacagcgc ataattgctg gaaaatacat gtattttctt
     5221 ctccaggcgc atttgtctca cctgaaacca gggctgcccc attctgatgc caagggcttt
     5281 tgcctccggg ctgcgcgcga tcacacagcc atcgttattg ctgagtacga tgaccggttc
     5341 gttgcgaagg tccgggcgga aaactttttc acatgaggcg tagaaactgt tgatatcagc
     5401 cagtgcaaac atcagcgtaa ctcccgcgtc ctgtgtatca cgtgagtgac aataccaaaa
     5461 atacagatgt tttccggata cagtgtgcgg aactccgggc tgtctgaaac cggctccagt
     5521 gccgggcgtg ggcgcaacag cagtcgtttg acggtgaact caccgtcgat ctcagcgata
     5581 acgatgtccc cgtgttgtgg tttttcggcc ctgtccacta ccagcagatc accattctgc
     5641 acgccagcct ggttcatcga ttcaccgctg gtgcgcagaa agaaggtggc tgcaggtctg
     5701 ctgatgcaat agctgttcag atccagttcc tgctcagcat aatcagtggc aggcgaagga
     5761 aaaccggcct ggcaacgatc ggcaaacaac ggtcgcacgt aactgtcatc gcctgaaggg
     5821 tcagccggac ggtgataaac ggtactcatg ctgcgggcag ccagctgtcg tcctgccaga
     5881 tttcttccag cagagactgg atacgttctt tacccttgtc gttgcgggct ccagtgacag
     5941 atacagacgg ggcgctgccc tggtctatac gcagccagat ttccgggtag tgtggtttca
     6001 gacgttgttc gatttcattc tgtagcgcct gaagtgtgcc tgatttgagg ttttttgtgc
     6061 tctggcggtc gaaaagaatt tcaatgcgga tcatgttctc atctcccttt tcttttgctg
     6121 tatgtatatg ggtcttcccc tgttgtggtg ggtggtacat aattgagtga ttttcaaaca
     6181 ccacacagta cctttatggt gtctcttttt acatgagagg gaattcttat gagtgtgccg
     6241 caaacaaaag ctgaactgct tttagctatt gataaaaatt ttagtaaatt aattagttac
     6301 ctcaacacaa tcccaccaga aattacttca gataaatcaa tggacggaca cgccaaagga
     6361 acggagatga gtgttcgtga tctcgtttcg tatctgcttg gatggaatgc tcttgttgta
     6421 aagtggatcg cttctgatgc taaaggtctg cctgtcgatt ttccggaaac tggctataaa
     6481 tggaatcagc ttggccttct tgctcaaaaa ttttactcag attacagtga gttaagttat
     6541 gagttgttag tagctgaact tcagactgta aaaaatgaga ttgtgaacct tattaatgat
     6601 cgtaccgatg atattttgta tggaagacca tggtacacaa aatggacgat ggggagaatg
     6661 atctcattta acacatcttc gccttacgcc aacgctaatg gaagattaag aaagtgggca
     6721 aaaaataata atatcagttt aaagtaagct tcagaatgaa attatatgga cgaaaaatcc
     6781 ctctatgccc atatccttaa cctgactgca ccatggcagg ttaaatccct taccctcgat
     6841 gaaaatgcag gttccgttac tgttacagtc ggaattgctg aaaatactca gttaacctgt
     6901 ccgacctgca ggaaatcctg ttctgttcac gatcaccgac atcgtaaatg gcgccacctt
     6961 gatacctgcc agttcatgac attagtagaa gccgatgttc cccgcgttat gtgcccggag
     7021 catggctgcc agactctgcc tgtaccgtgg gcaggttccg gcagtcggta cactctgctg
     7081 ttcgaatcgt tcgtgctctc atggcttaaa atcagcaccg ttgatgcggt cagaaaacaa
     7141 cttaaactta gctggaatgc cgttgacggc atcatgaccc gcgcggttaa gcgaggcctg
     7201 tcgcggatta aaaagccttt atcagtgcgt catatgaatg tagacgaagt cgcctttaaa
     7261 aaagggcata ggtatataac cgtggtatct gatcgcgacg ggcgggcact ggcattaacc
     7321 gatgatcgtg gcacagagag tcttgccagc tatctccgtt cgcttactga cagtcagttg
     7381 ttggccatca aaaacactgt cgatggacat gaatgcgggc tatataagag cagcgcgtat
     7441 ccatttaccc aatgcggtcg agaaaatcgc cttcgatcgc ttccatgtgg cgaagcaact
     7501 gggcgaagtg gttgataaaa cccgccagaa tgaacatccg cacctccctg ttgaaagtcg
     7561 tcgtcaggcc aaaggtaccc gcttcctgtg gcagtacagc gacaaatgga tgactgagtc
     7621 ccggcaggaa aagctgatgt ggttgcggga acagatgcaa cagacaagcc agtgctggac
     7681 actgaaagag ctggcaaaaa atatctggga tcgccctgga gcacagaacg caggaatgac
     7741 tggttgcagt ggatatcgcg ggcgtctgaa tgtgatgtgc cgatgatgaa aaacgcagcg
     7801 aaaaccatta aaaaacggtt atacggaata ctgaatgcaa tgcgtcatcg cgtctcgaat
     7861 ggaaatgcgg aggcgctgaa cagcaagatc agactgctga ggataaaggc caggggatac
     7921 cgaaaccggg aacgctttaa actgggagtt atgttccact atgggaaact gaatatggcg
     7981 tactgagtct tcccaccatg atcggggaag acccatgtat atacagtatt ctaataactg
     8041 aatccacaca cagtcaaatg tcattattgt gactaaaaaa cgctcaattc tctgagggtg
     8101 tggctgtatg cctgctgtgg actgctgtgt ccagcttatc cacagcattt tgtgcacggt
     8161 tctgtggaca aaatacttgg ttacccaggc cgtgccggca cgttaatcgg gctgcatccg
     8221 atgcaagtgt gtcgctgtcg ccggcctcct cacccggtca cgtttcgtcg ttcctcctcc
     8281 acgcgctgcg gcttcggggc cgcacctgca ttcgtatgcg gtcgcccggt tacaggtgcg
     8341 gcacggcctg atggaggccg tatgtgagag gagaattccc atgccaaact ggtgctcgaa
     8401 tcgtatgtat ttttctggtg aaccggcaaa gattgctgag attaaacggc tggccagcgg
     8461 tgcagtcaca ccgttttatc gccgcgccac aaatgaaggt attcagctgt ttctggccgg
     8521 aagtgccgga cttctgcaga ccactgaaga tgtgcggttt gaaccatgcc ccggactgac
     8581 ggctgccgga cgtggtgttt tatcgccgga gaatatcacg ttcacccgct ggctgaagca
     8641 cctgcaggac ggtgtactgc tggatgagca aaactgcctg atgctgcatg aactctggct
     8701 gcagagcggt actggacagc gtcgctggga agggttaccg gatgatgtca gggaaactat
     8761 cactgtgcat ttcaccgcaa aaagaggtga ctggtgtgac atctggggta acgaggatgt
     8821 atcggtgtgg tggaaccgtc tgtgtgacaa cgtactgtcg gaaaaaacca tgccgtttga
     8881 cctgctgacg gttttgccga cccgcctgga tattgaagtg aatggcttta acggcggtgt
     8941 tctgaacggt gttccttctg catatcactg gtatacagaa cggtatggcg tgaagtggcc
     9001 ttgtgggtat gacctgaata tcagcagtca gggggaaaac ttcattcagg tagatttcga
     9061 cacgccgtgg tgtcagccgg aaagcgacgt tgttgcagaa ttaagccgcc gtttcggctg
     9121 cacgctggag cactggtacg ccgaacaggg ctgcaatttc tgtggctgtg tcaacgccac
     9181 gatgtttgac cgttatttgc cattttcatg cccgctaaga tactttcgtt actgatgctg
     9241 tccgcttttc accatttcat catcactgat tggcgttgtg tttttggtca gcactccagc
     9301 tttacgtttc tctttaaa
//
