LOCUS       NZ_CP069490            11357 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1264 plasmid unnamed2.
ACCESSION   NZ_CP069490
VERSION     NZ_CP069490.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357406
            Assembly: GCF_016889265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11357)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11357)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 990.89x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 04:51:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,921
            CDSs (total)                      :: 4,808
            Genes (coding)                    :: 4,621
            CDSs (with protein)               :: 4,621
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 187
            CDSs (without protein)            :: 187
            Pseudo Genes (ambiguous residues) :: 0 of 187
            Pseudo Genes (frameshifted)       :: 73 of 187
            Pseudo Genes (incomplete)         :: 130 of 187
            Pseudo Genes (internal stop)      :: 34 of 187
            Pseudo Genes (multiple problems)  :: 45 of 187
            Pseudo Genes (short protein)      :: 3 of 187
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069490.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1264"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1264"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..199)
                     /locus_tag="I6J94_RS24155"
                     /old_locus_tag="I6J94_24160"
                     /pseudo=""
     CDS             complement(<1..199)
                     /locus_tag="I6J94_RS24155"
                     /old_locus_tag="I6J94_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /name="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
     gene            432..764
                     /gene="mobA"
                     /locus_tag="I6J94_RS24160"
                     /old_locus_tag="I6J94_24165"
     CDS             432..764
                     /gene="mobA"
                     /locus_tag="I6J94_RS24160"
                     /old_locus_tag="I6J94_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_001291058.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTF
                     IRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALR
                     RDLMGR"
                     /name="mobA"
     gene            776..3487
                     /locus_tag="I6J94_RS24165"
                     /old_locus_tag="I6J94_24170"
     CDS             776..3487
                     /locus_tag="I6J94_RS24165"
                     /old_locus_tag="I6J94_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000991381.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVP
                     HRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKA
                     DKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAIHTDTDN
                     LHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRIVRKT
                     ALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGEL
                     VVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVD
                     IRPEKVAETESLKQYACRYFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVL
                     HDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAV
                     SDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNFSWQACHEMF
                     ARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAELQAGPFEIAGADIFERV
                     KPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRKPDLRY
                     GERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIA
                     EGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNADRCVILCEPGGTP
                     LYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDRNELVDKLNLIAPVL
                     FDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISRPDVDLHRQRSEQYYH
                     EYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3555..3986
                     /locus_tag="I6J94_RS24170"
                     /old_locus_tag="I6J94_24175"
     CDS             3555..3986
                     /locus_tag="I6J94_RS24170"
                     /old_locus_tag="I6J94_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="WP_072264695.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTAL
                     GSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKISTSN
                     GDKTYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
                     /name="cell envelope integrity TolA C-terminal
                     domain-containing protein"
     gene            complement(4084..4314)
                     /locus_tag="I6J94_RS24175"
                     /old_locus_tag="I6J94_24180"
     CDS             complement(4084..4314)
                     /locus_tag="I6J94_RS24175"
                     /old_locus_tag="I6J94_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065406859.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALINI
                     TDRKNEGTNTIDGNWQADERRRYRDNVRIYF"
                     /name="hypothetical protein"
     gene            complement(4781..7084)
                     /gene="trbC"
                     /locus_tag="I6J94_RS24180"
                     /old_locus_tag="I6J94_24185"
     CDS             complement(4781..7084)
                     /gene="trbC"
                     /locus_tag="I6J94_RS24180"
                     /old_locus_tag="I6J94_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="WP_000077664.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIWP
                     LALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEA
                     DETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNPLCW
                     GRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSRPQSN
                     TWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQ
                     MLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQ
                     HAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSAL
                     GRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEAT
                     QVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTARTVKNAAGE
                     EARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFTVFQGDVVPSA
                     SVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSIIGVLTA
                     KPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNT
                     TREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLTASPPHKKNVAQ
                     TPSGGKQESRKKRF"
                     /name="trbC"
     gene            complement(7065..8189)
                     /locus_tag="I6J94_RS24185"
                     /old_locus_tag="I6J94_24190"
     CDS             complement(7065..8189)
                     /locus_tag="I6J94_RS24185"
                     /old_locus_tag="I6J94_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="WP_000152381.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNNR
                     LMHLSFSSPFREITEAVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKYGF
                     WRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTEHHV
                     NSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINLSTGH
                     ERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCEPDSQK
                     RATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTPWVLSDT
                     GWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
                     /name="DsbC family protein"
     gene            complement(8186..9538)
                     /locus_tag="I6J94_RS24190"
                     /old_locus_tag="I6J94_24195"
     CDS             complement(8186..9538)
                     /locus_tag="I6J94_RS24190"
                     /old_locus_tag="I6J94_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="WP_180482026.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFALLIWLFLPDIVFATCLIL
                     HTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMTIG
                     FLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPERRVA
                     LTPEAFVEQNNLIRNMQLDVAATRQCFMAQLGQPLTTWKDMAPHEKALFAVFGLQFFLG
                     DRKSAVSLMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEDAQQWLRQHRYVR
                     SGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARAENEASR
                     LGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVIPRRHDND
                     EDTDTGETTETTETTETTETTETTETTETTETTETTETTEKQHPADKEKEQ"
                     /name="conjugal transfer protein TrbA"
     gene            10335..10430
                     /gene="dqlB"
                     /locus_tag="I6J94_RS24765"
     CDS             10335..10430
                     /gene="dqlB"
                     /locus_tag="I6J94_RS24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_001363141.1"
                     /translation="MINLLIIVLRAVVAVANAVIALLELIRELVD"
                     /name="dqlB"
     gene            complement(10493..10669)
                     /locus_tag="I6J94_RS24200"
                     /old_locus_tag="I6J94_24205"
     CDS             complement(10493..10669)
                     /locus_tag="I6J94_RS24200"
                     /old_locus_tag="I6J94_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001054907.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSRE
                     QAQEVQEGFRFLT"
                     /name="hypothetical protein"
     gene            complement(10819..11286)
                     /locus_tag="I6J94_RS24205"
                     /old_locus_tag="I6J94_24210"
     CDS             complement(10819..11286)
                     /locus_tag="I6J94_RS24205"
                     /old_locus_tag="I6J94_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_000598962.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVRV
                     RLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEANRW
                     QVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
                     /name="thermonuclease family protein"
     Typing          333..431
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          777..3484
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.287"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4785..7084)
                     /name="MPF_I"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.348"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cataccctgc atttttgagc gtgtcacgaa catctgccag tgtataaccc tttgtcttta
       61 ccaggacaat aatgtcatca cgaatggcat caagaaaatc acgcaatgtt ttccgttgag
      121 cggtgagatc cggcaattcc gataaggcag cttttgccag ctggatgtca tcatctgagt
      181 aaaatttttt agaagccatt ggcactttct ctccgtttca tgttgaagtc tgattttatc
      241 atcaaaaact gcctcctggc agggctgcgc cctgcctctg ccgtctgcat aagactatga
      301 tgcacaaaaa taacaggcta taatggcctg aaaaacggga caggatgtgg gaatgtcgga
      361 ccgcgcacgc gcactcagac attccccacc tggtcagggc tttgccccga caccccggta
      421 aggagcagac catgagtgag aagaaaaccc gaacgggcag tgagaacagg aagagaattg
      481 taaaattcac ggcgagattc acggaggatg aggctgaaat cgtaagagaa aaagcggagt
      541 caagtggaca gacggtttca acatttatac gaagctcttc actggataag gtggttaact
      601 gccgtactga cgaccgtatg attgatgaga ttatgcgtct tggccgcctt cagaagcatc
      661 tgtttgtgga gggaaaacgc actggtgata aggagtatgc agaggttctt gttgccatta
      721 ctgaatatgt caatgctctg cgaagagact taatgggacg ctgagataac tgctgatgaa
      781 tgccattatt ccgcacaaaa gacgtgacgg caggtcttcc tttgaagacc tgattgcgta
      841 cacgtctgtg cgggatgatg tgcaggaaaa tgagctcaca gaagacagcg aaatcaagag
      901 tgatgtccct caccgcaacc gtttcagacg gcttgttgat tatgcaacac gcctgcgtaa
      961 cgaaaaattt gtctccctga ttgatgtgat gaaagacggc agtcagtggg taaatttcta
     1021 tggcgtaacc tgtttccata actgcaattc actggaaaca gctgctgaag agatgcagta
     1081 caaggcggac aaagctgttt tttcccgtaa tgatacagac cccgttttcc actacattct
     1141 ttcctggcct gcgcatgaaa gcccgcgtcc tgagcagctt tttgactgtg tgcgtcacac
     1201 actgaaatct cttgaactct caaaacatca gtacgtggct gccattcata cggacacgga
     1261 taatctgcat gtgcatgttg ccgtgaatcg tgtacatcct gaaactggtt atattaactg
     1321 gctttcctgg agccaggaaa aactcagccg tgcatgtcgt gaacttgaac ttaagcacgg
     1381 attcgcgcct gataatggct gctttgtgca tgcacccggc aaccgtattg tgcgcaaaac
     1441 agcgctggta cgtgagcgcc gtaatgcctg gcgacgcggg aaaaagcaga cgttcaggga
     1501 atatattgcg cagatgtcga ttgccgggct gcgtgaagag cccgcacaag actggttgtc
     1561 tttgcataaa cggctggcca gtgatggtct gtatatcacg atgcaggaag gggaactggt
     1621 ggtgaaggat ggctgggaca gagcgcggga aggtgttgca ctcagttcgt tcggaccgtc
     1681 atggacagct gaaaaactgg ggcggaaact gggggaatat cagcctgtac cgacagatat
     1741 tttcagtcag gtggggacac ccggtcgtta tgatccggaa gccataaacg ttgatatccg
     1801 gccggaaaaa gtggcagaaa cggagagtct gaaacagtac gcctgccgtt attttgccga
     1861 acgcttaccg gaaatggcga gaaatggtga gctggaaagt tgcctggatg tgcacagaac
     1921 tctggcaaag gctggattat ggatggggat ccagcacggg catctggtac tgcatgatgg
     1981 atttgataaa caacagacac cagtacgtgc agacagtgtg tggccgttaa tgacgctgga
     2041 ttatatgcag gatctggatg gtggatggca gcctgtaccg aaggatattt ttactcaggt
     2101 tatacccgga gaacgattcc ggggacgtaa tcttggtact caggctgtca gtgattacga
     2161 atggtatcgt atgcggatgg ggacaggtcc acagggagca ataaagcgcg aactgttttc
     2221 tgataaggaa agtctgtggg ggtacacgac tgttcagtgt gagtcgttga ttgaagatat
     2281 gatcgcgggc gggaatttca gctggcaggc gtgccatgag atgtttgcgc gtaaagggct
     2341 tatgttgcag aaacagcatc atggtcttgt gattgtggat gcgtttaatc atgagctcac
     2401 accagtgaag gccagcagta tccacccgga tttgacgtta tccagggctg agctgcaggc
     2461 agggccattt gagattgcag gtgcagatat atttgaacgg gtgaagcctg aatgtcgcta
     2521 caatccggaa cttgctgcca gcgatgaagt ggaacccggc ttccggcgcg atcctgtact
     2581 gcgtcgtgaa cgccgtgaag cccgtgcagc tgcgcgggaa gatttgcgcg cgcgttatct
     2641 tgcatggaag gagcactggc gtaaacccga cttacgttac ggagaacggt tacgggaaat
     2701 tcatgctgcc tgccgtcggc gcaaggcgta tatccgtgtg cagttccggg atccacaatt
     2761 gcgtaagctg cattaccata ttgcggaagt gcagcgtatg caggcgctga tcaggctgaa
     2821 ggagagtgtg aaggaagagc ggttgtcact gattgcggag ggtaagtggt atcctttgtc
     2881 ctaccggcag tgggtggagc aacaggcggt acagggagac agagctgcat tatcacagtt
     2941 acgtggctgg gattatcgtg atcgtcgcaa agacaagcga cgaacaacga atgccgaccg
     3001 ctgcgtgatt ctctgtgaac cgggaggaac tccactttat gaagacactg gcgttctcga
     3061 agcccgtctg cagaaagatg gcagtgtgcg ttttcgtgat cggaggaatg gcgagttagt
     3121 ctgtgtggat tatggggacc gtgtggtgtt ctaccatcat caggacagaa atgaactggt
     3181 ggataagctg aatctgattg ctcctgtatt gtttgatcgt gagccaggaa tgggttttga
     3241 accggaaggc tcatatcaac agtttaatga tgtatttgcg gaaatggtgg cctggcataa
     3301 tgcagctgga ataaccggaa atggacactt tgtaatcagc cgcccggatg tggatttaca
     3361 tcgtcagcgt agtgaacagt actaccacga atatatcagg caacagaaaa gcatatccgg
     3421 ggggcatggc gcatcttatg ctccagttca ggataatgag tggacgccac catcacctgg
     3481 aatgtaggga tgaggagtgg atattaaaaa tcttaacttg tattagttaa gattttcttt
     3541 gctctgacag agctatggta ctgagatcta tatggattga ggagcaatat atgggggtaa
     3601 agaaaatcgt tatgctggct attttagtca gctttgtggc gggatgtgcg ccattacatc
     3661 cttcaaattg ccataaaact acagccctgg gtagttgcag ttcaggacgt tgggatgatc
     3721 aggatgaatg gggggagcag gcacggggaa tcagggatgc aattaatgct aaacttgatg
     3781 atccgcaaaa atggaaaagg aaaaaatgca ggttgcatat ggaattctcc cgggatggca
     3841 cggcgttaaa aatatcaacc agtaatggcg ataaaaccta ttgcgaagcg ataaagtcag
     3901 cagcccataa agctaaattc ccggccttca acaatccgga agtttataga gattttcaaa
     3961 aatccggctt tgacatgcgc ggttagctcc acgacatatc tgtagggcta acagaagact
     4021 gtcaggttgg ctgattacta cactggaacc tgaggtactt tgcctcaggt tatgttgaca
     4081 tactcaaaaa tagattctta cattatcccg gtaacggcgt ctttcatcgg cctgccagtt
     4141 accatcaatg gtattggtac cctcgttctt tctgtctgtg atgttaataa gggcaaaatt
     4201 aatcagcatg tctggcagga tctgggaatt cataccaata tccatagaag tgaatctatt
     4261 acgaacgtgg ggaaccctgg caccattttt tgtgctgccc atatttgttt tcatgtataa
     4321 ttcataatca aagcgttcat cctgatttag cttaactgat taaaatccgt agattatgat
     4381 tttatcagtg ttgtgacttt atttattatg gggagcttac ccagtatttc gaaattatcg
     4441 ggagaacact tgtaccatct gtttgagcct gcaagtattt ctctgtccca tcgtctgacc
     4501 ctctccccgt ttctgagtat gcatatcata tagttttcgg gcaccatcag tgtccgggct
     4561 gccagaagac tatttttaaa cgtcagggaa gtgatattga tatcttctcc ggaattttta
     4621 cgataaataa cagaccatga gtttgtttcc gggctactaa tacacattta ttgtacctct
     4681 ggtgatggat tgaaatcctg aatcctccgc ttactatggt gagcggagga gattaataat
     4741 cgcgtaaagg ttgttattaa aatcagccat acaggtgtta tcagaatctt tttttacgac
     4801 tctcttgttt tccacctgat ggtgtctgag cgacattttt tttatggggc ggggatgcag
     4861 ttagatccgg tacaggtaac agcatctcca gggggagcag agtattcaga atcgccttca
     4921 ctgaaggtat atcttcagga ccagagtgtt cctcttccgg acgattcagt gtgatataac
     4981 atacccggcg ttcttccctg gttgtgttat tgatagtgtg cacagccttt ttccacagtt
     5041 cgtttgcccg ggattcgata tctgtatcat attgcggaag cagatccagt gatgtctgcg
     5101 ctgttgccaa ccggcgctgg aatgtatcaa tgatacggta aggctctgta cggtttttac
     5161 gacgtttacg ggatggtttt gctgtcagta caccgatgat gctgctgaca tgctccggtg
     5221 tggggagcct gcgctgcact gtcctgggga cgagacggcg gagacgctcc agccgggggg
     5281 cttcaacgga aatatagcgg ttgatgacca ccggatcact gtcgcaactt ttctcgctgt
     5341 ccggaatata cacagaggct gacggcacca catccccctg gaaaacggta aagaactcac
     5401 cggcattcag tctgatcaaa tcctgcacgt cgattttgct ttccatctgg atggtgatct
     5461 ggttaccgtc cacccaggac tcacccatca caccatcgtg gcgctgcagg ctgcccattc
     5521 tggcccgtgc ctcttcaccg gcggcatttt tcaccgttct tgccgttttg tcgtcactga
     5581 caatgcgtcc ggcgaatttg gtgccggcgt tctgcatcag tgtggcggtg ttggtggcgg
     5641 aggtctgccc ttcaattcgc tcctgatcct gcgccgtcat tatcagggaa aactccagtg
     5701 aacgtacctg ggtggcctcc acagcaatgc ggtctgtgta gtaggcaccg acttcgtcaa
     5761 ggatacagat ataggggaag ctgcctttgt attttttgac ttccagggtc tgtgcatctg
     5821 tgccttccag ccggtagccc aggtcacggg cgaggagcat gctctgctgg gtgacgaaca
     5881 tacgaccgag tgccgacgtg gtatgagccg atgtgtccag tgcagggatc atcacaatca
     5941 gaatgcggtc gctgtgaata ctgtcgcgaa tatcgatgtc gccggcatct gccgcaaaga
     6001 cgtccccgaa ggtttcggca aaggttgtga acgtttccga gaactgaccg gagagataag
     6061 catgctgttt acggggctcc tcagtccagg ctgacggcgt tctcaccagt gacatatcga
     6121 atcccgggac atcctgcagg tagtttcgca gtggtgcgat agcttcttcc ggccactggt
     6181 catcaaggcc acggcaatag agttttgcca tcccttccag cgtcatatgt tcccgtaaca
     6241 tctgcaggga catggtttta ccttcccgga cgcaccagaa ttttgtgcca aagaccaggg
     6301 ccttgttcat ggctatcgcc cgtgactgcc attcaccgcc ctgaacgttc tggggcagca
     6361 tggactgcat ggtttcggcg gtgaaggctt cggtgctgaa tgcgaacggg ttccaggtgt
     6421 ttgactgtgg gcgggatttt tcgccgctct gaatgatttc actgcgggat tttccgccgt
     6481 tcatgaagtt gatgacctcg atatcatcct ccctgccaaa acgtcgggag agataccaga
     6541 ttgtcctcgt ggtatcgttc tgtgcctttc cgtcaacaaa ggtaaagcct cttccccagc
     6601 agagcgggtt gagcagccag gcaaaggtgg tttctgtttt accgccaccg gtggtggcaa
     6661 aaaacatgac atgccgggtg aggtcatcca tgctcagcca gagctcacga ccgatatcgt
     6721 tgatacggcg gtaaccgaca taaaaaatcc cccgggcggg gaatgtttca tccgcttcat
     6781 actgaaacag ggtgggccag aagctgaaga ggcttctcct gacctttctg tcctgggagg
     6841 ggtcatcaag gctgagtgtc atgggcatac gcagggggca tcgccagcgt tgcagcatga
     6901 atatcacgga ggtgaccagg gtgaacagca ggcagacagg aagggccagt ggccatatca
     6961 gcgccgtaat gatactgccg gccagacaca gaccagtgat gttcaggtta tgcagtgcat
     7021 tccacaacgg gtttccccag gccgtgcggt gtatgaggtc aggattaacg tggtgctggc
     7081 tcattgtgat actcctcgct atctgttgct ttcaggaaga ggcgaacgga agtggcgtct
     7141 ttcaggatgc cggacggcag gtgccagccg gtatctgata acacccaggg ggttcctcca
     7201 aaaccgaact ggcggaaagc ctggttattg acatcaatgg cagcacgtgc ggcggtcaga
     7261 cactgtccat cctgtgcggc cggtgcatcg ccggtggggg ccataccact gtcagcggaa
     7321 tacagctgat gccatccggt cgcccgtttt tgtgagtccg gttcacacag cagcggagca
     7381 gtccgctttg tactggactc cccgccaatc acggagaccg gataaatcac cacgctgaat
     7441 tcccgggcca gttgcttaag cgcgggttcc agcagacggc agttgatgca tgcggggtca
     7501 aggaaggcgt acagcgtgcg ttcatgacca gtggacaggt taatggtgaa catcccccgg
     7561 tctgcgttgc gtttaagtac tgtggccaga ttacggcggg cctcagcggc ctgaacgtgt
     7621 tgttcttccg tcactgccgt cggagcgggg gcagaagaag gttcctgctg tgtgggagcc
     7681 gggcgggccg ggatgctgtt aacatgatgt tccgtatgtg ggcgtggtgt ctctgtgtcg
     7741 gttactggtt cagctctcca ttcctgagat gtagggtaag ccagcgtgga agggggctct
     7801 gccttcatcc agtggtaacc tgcaactgtc gccggcagag caattccggc gataaagcca
     7861 cacatcagtg tccgccagaa gccatatttc cgggagggat gttttccttt tccggcatat
     7921 tcggcatcca ggcaggcatc aatcgctgag agcattctgc cgatgtgatg tccgggacgt
     7981 tctgcatcaa cggtatcaac aagcaccgct tcggttattt cgcgaaaagg ggaactgaag
     8041 gacaggtgca tcagcctgtt attacggtta atcagtgcgc ccagttctgt gaccggcagg
     8101 atggcaatca cctcgcgatt accgtcaggg tacagggcgg tcagggacag gtgtttatta
     8161 ttcagtgttg cagtgtagtg gatggtcatt gttctttttc cttgtcggcg ggatgctgtt
     8221 tttcggtggt ttcggtggtt tcggtggttt cggtggtttc ggtggtttcg gtggtttcgg
     8281 tggtttcggt ggtttcggtg gtttcggtgg tttcggtggt ttcaccagtg tcagtgtctt
     8341 cgtcgttatc atggcggcgg ggtatcacgt catccgtgag tgaccagcgg gtcctgatct
     8401 gacgtttacg atcgcgatct ggctgaggtt cgtcccatat caggcccagc cccagcatgt
     8461 cccgtcgaag cccttcaatg gcctcttcca cacagggttc cgggcagggg aggccgagac
     8521 ggctggcttc attttctgcc cgggcaacgg ccaccacgcc ggccccttcg atgaagcctt
     8581 ttgtggtgtt tgcccggtga agggcataga agagcgtccg gtcaacgccc ttgagccaca
     8641 gccagttggg gggcgtcagg cgcaaatcat gtgcgtaaag ccagacaaga ccactgcgaa
     8701 cgtaacggtg ctgcctgagc cactgttgtg cgtcttctgt ttttatgacc cggataaagg
     8761 cgtttctggc cagtgaatac actggcgtgc tgaaacgccc ctggtcccgc ttgcttttga
     8821 gtctgcagga gaggttgagg ttattcatca gggaaacagc cgatttccgg tccccaagga
     8881 agaactgcag gccaaagacg gcaaagagtg ctttttcatg tggagccatg tctttccagg
     8941 tggtcagagg ctggcccagt tgcgccataa agcactggcg cgtggccgca acatcgagct
     9001 gcatgttgcg gataaggttg ttctgctcca caaaggcttc gggggttaga gcgacacggc
     9061 gctcgggacg tttttgtccg tggaagagtc tgttgctgtc tccgtcagcc agaacggggg
     9121 cgatcgccgg tgacagtgat gaaaacagat gtggcagcgt gtggatattc agtggtctgc
     9181 gggtgaagcg gggctgggcc ggatgctgaa accacatcca cagaaagccg atcgtcatgg
     9241 gaagcagaat cagccagagg atccccacgg tctgtgacat gacatccagc cactggctga
     9301 aagtgatcgt tgcagcgttg ttggcggtgt tggccagcag gttataacgg ggggcggcaa
     9361 aatggtgaaa aggcccccag tcagtcatcc cccacattgt gtgcaggatc aggcaggtgg
     9421 caaagacgat gtcgggtaaa aagagccaga tgagaagcgc aaagagcaga catatggtgc
     9481 caacgatata ccatatgttc atcgggtctt ctgttgtggg ggagtgttgg taagacacga
     9541 ggattcctcc atcgataaat agataaattt cgggatgagg ctgagtggaa ttaatgcgaa
     9601 caatcgccgg taatgaccgg gcagggctga attcagcagg gcattttcac aggaacagca
     9661 gaaggtggtg agagacaggc acaaaaaagc cgactggtga ggccggcttt tttgcgatcc
     9721 cagggagagt gggtggctct ggcccggagg aagtctcttt tcaactaacg ttaccagaat
     9781 atcgttatat tcacctgatc gcaatctgtt cgttacagtc tattgttccg ggagtagcga
     9841 tatcgcctga aacaagtata atcagcaagt tagccttgtc tggagactta ccttcgcatc
     9901 aatccatttg ccgtattttc gtaaaattca acaggaaata ttcaacggga gtgagagaac
     9961 tgctgtccgg ggaggaggta cagacgcaaa aaaaccgact ggtgaggccg gtttttttgc
    10021 gattccggat cgaagttggt agccctgacc ggaagataac tgcctataac tgactatgaa
    10081 ataatacggc tgaaataata tttctgcaag cacagtataa gtatatactc ggtgtgtcag
    10141 ctttgaggca cgttctttcc ggcatatcgt tcaccgggat gaacaggttg caagtcctgg
    10201 ccaaaacatc aggtggtgct ggttttggtg ctctggccgg agaagaactg tctataactg
    10261 acactataga aaacgtggcg acaggcaatg taagggcaaa ccacggtgtt ctttctccat
    10321 tgatggagaa gcggatgatt aacctgttaa tcatcgttct cagggcggtt gttgctgtgg
    10381 ctaacgcagt gatagctctc ctggaactga tccgggaact ggttgactga taatcccgga
    10441 aacgtactta agggcagagg gtcaccgcct tctgcccttt aacaaaatat gctcaggtta
    10501 aaaatcgaaa accttcctgc acttcctgtg cctgttcccg tgagtcaaac atgagccggc
    10561 ttttacccgg aactccccag gaaatatatt ctacatccac ccaccatttt ccgtattctt
    10621 catacggtcc ggatatcact ttagtgacaa atgcatcaac caggttcatt tttacacctt
    10681 tctgttattc tgcttattac gaccagcaga cagcaaatat taaaaataac gcggcggtaa
    10741 aaatggcaat tttttccatg ctgtattttt gccatttatt ttctttgaaa tggttttttt
    10801 cttcatatca gcaaataatt atttcttatt ctgttttctc caggtccatg gtggtaccgg
    10861 atttttatct gcccataatc cccgcttcag ggcgcgggca tcacgctgga gcggcgggag
    10921 agtatagtca ttattgtagt ccgggtacac ccaggcagcg ccgttacgga cctgccagcg
    10981 atttgcttct gtgccgtcag cggtatagac ccggccgaga atacggccat acctgtctgt
    11041 ctgcgcgtat tgcacggtta cgggctttcc ggccaccagg tccttcagct gagtggctga
    11101 ccagcgtcca aagggctgtt tcttttccgg tgcatcaata ttagccagac ggaccctgac
    11161 aggctttttg tcctgtaata tttccagcgt atcaccatca agaacgcgga tgacgtgccc
    11221 ctgaatctgt gcggcacaga gagaagcgga aaaaataatg gtgagtgaaa agatatattt
    11281 tatcatttca tcgtctcctt tttatttatg aaaacaggtg caattaatat tgcgaaataa
    11341 taacgttcag gtgtttt
//
