LOCUS       NZ_CP069477             6266 bp    DNA     circular CON 02-APR-2024
DEFINITION  Staphylococcus epidermidis strain FDAARGOS_1243 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP069477
VERSION     NZ_CP069477.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357385
            Assembly: GCF_016889165.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Carlson,P., Fischbach,M., Hastie,J., Bilen,M., Cheng,A., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Carlson,P., Fischbach,M., Hastie,J., Bilen,M., Cheng,A., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 3182.86x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016889165.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 04:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,447
            CDSs (total)                      :: 2,363
            Genes (coding)                    :: 2,300
            CDSs (with protein)               :: 2,300
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 34 of 63
            Pseudo Genes (incomplete)         :: 37 of 63
            Pseudo Genes (internal stop)      :: 22 of 63
            Pseudo Genes (multiple problems)  :: 24 of 63
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069477.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1243"
                     /culture_collection="FDA:FDAARGOS_1243"
                     /db_xref="taxon:1282"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MD"
                     /collected_by="FDA-Stanford Microbiome Effort"
     gene            10..873
                     /locus_tag="I6J73_RS12135"
                     /old_locus_tag="I6J73_12140"
     CDS             10..873
                     /locus_tag="I6J73_RS12135"
                     /old_locus_tag="I6J73_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440631.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_145366611.1"
                     /translation="MSGETVIYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLHL
                     SFDELKELSNYSPETRNINRFVDDLQRVYDKMLDIRYTIRTETKIKKFVLFYKYEIDIS
                     EKYLEIATSPDLKYILNSITNNFTKFELKEMTHLKSTYSKNMFRLLKQYKHTGYMKIKI
                     EDFRERLDIPKSYRMSNINQFVLTPIIKELSPIFNNLNINKIKAKKGRKIEWLEFTFDA
                     EKRIHSKRQPQMTDVGKSRQYISREKTPKWLEERTYEKTTQNEYDPQLEKEREAFLKQL
                     QVDWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(1008..1103)
                     /locus_tag="I6J73_RS12140"
                     /old_locus_tag="I6J73_12145"
     CDS             complement(1008..1103)
                     /locus_tag="I6J73_RS12140"
                     /old_locus_tag="I6J73_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001002795.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGYWLHKRDKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1527..3095)
                     /locus_tag="I6J73_RS12145"
                     /old_locus_tag="I6J73_12150"
     CDS             complement(1527..3095)
                     /locus_tag="I6J73_RS12145"
                     /old_locus_tag="I6J73_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449123.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vga family ABC-F type ribosomal protection
                     protein"
                     /protein_id="WP_145366609.1"
                     /translation="MKIMLEGLHIKHYVQDRLLLNINRLNIYQNDRIGLIGKNGSGKTT
                     LLHILYKKIVPEEGIVKQFSHCELIPQLKLIESTKSGGEVTRNYIRQTLDKNPELLLAD
                     EPTTNLDNNYIEKLEQDLKNWHGAFIIVSHDRAFLDNLCTTIWEIDEGRITEYKGNYSN
                     YVEQKELERHREELEYEKYEKEKKRLEKAINIKEQKAQRATKKPKNLSLSEGKITGAKP
                     YFAGKQKKLRKTVKSLETRLEKLESVEKRNELPPLKMDLVNLESVKNRTIIRGEDVSGT
                     IEGRVLWKAKSFSIRGGDKMAIIGSNGTGKTTFIKKIVHGNPGISLSPSVKIGYFSQKI
                     DTLELDKSILENVQSSSQQNETLIRTILARMHFFRDDVYKPISVLSGGERVKVALTKVF
                     LSEVNTLVLDEPTNFLDMEAIEAFESLLKEYNGSIIFVSHDRKFIEKVATRIMTIDNKE
                     IKIFDGTYEQFKQAEKPTRNIKEDKKLLLETKITEVLSRLSIEPSEELEQEFQNLINEK
                     RNLDK"
                     /name="Vga family ABC-F type ribosomal protection protein"
     gene            3645..4028
                     /gene="mobC"
                     /locus_tag="I6J73_RS12150"
                     /old_locus_tag="I6J73_12155"
     CDS             3645..4028
                     /gene="mobC"
                     /locus_tag="I6J73_RS12150"
                     /old_locus_tag="I6J73_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_048668204.1"
                     /translation="MSEQNHFVASDATVGQNHKPNRKEPKQISFRVSESEYLKLKQSAE
                     TLNMSVPAFVKKKAQGSRLVAPKFDKETRQSIAKDLSRLGANANQIAKYCHQHQYDAPN
                     YSALERNISELRERLDDIWQQLN"
                     /name="mobC"
     gene            4010..5017
                     /locus_tag="I6J73_RS12155"
                     /old_locus_tag="I6J73_12160"
     CDS             4010..5017
                     /locus_tag="I6J73_RS12155"
                     /old_locus_tag="I6J73_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_145366607.1"
                     /translation="MATTKLSATKSTSRAINYAEKRAEEKSGLNCDIDYAKSSFKATRE
                     LYGKTGGNEGHVIIQSFKPGEVTPEQCNQLGLELTEKFAPNHQVAVYTHNDTDHVHNHI
                     VINSIDLETGKKFNNNKQALRDLRDFNDDVCREHGLSVPEKDTARLRYTQTEKAIADPN
                     TKSTAQYSWKDDIREAIDQSTATNMDEFKDHLSQYGIKIARVTPKSITYRHLAEDKKVR
                     GRRLGEDYNKGGIEDGFERQIQRQQQEREDDNDIQPKRPTSNRDESTERDTGVTQADWE
                     QFAQDTNELERSRQAAESARLAHEKARRDREERAREKQASRTIIDNDRDHGLEL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4983..5525
                     /locus_tag="I6J73_RS12160"
                     /old_locus_tag="I6J73_12165"
     CDS             4983..5525
                     /locus_tag="I6J73_RS12160"
                     /old_locus_tag="I6J73_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_202596020.1"
                     /translation="MTTIETTASNFNQATEITQKRFISVAKHYIERINNDNLKQDFQTA
                     IQEELEDVKSQTQNLVKQFQNHQSDLQKAHDDYKKTLDERIETNEIAIKQYDQAFNRLT
                     KGITAMFFIVALVMITFVVLSPLGDLLGVQHFYDWINHVLKTGHSAWRYLIVTLYLAPY
                     ILFSLLIYAILKAYERL"
                     /name="mobilization protein"
     ARG             complement(1531..3095)
                     /name="vga(A)"
                     /gene="vga(A)"
                     /gene_name="ABC-F type ribosomal protection protein Vga(A)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE"
                     /identity="99.81"
                     /coverage="100.0"
     ARG             complement(1528..3095)
                     /name="vgaA"
                     /gene="vgaA"
                     /gene_name="vga-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="lincosamide antibiotic; streptogramin
                     antibiotic; streptogramin A antibiotic; pleuromutilin
                     antibiotic"
                     /identity="99.23"
                     /coverage="100.0"
     Typing          complement(3439..3674)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.326"
                     /coverage="100.0"
                     /evalue="5.88e-114"
     Typing          11..873
                     /name="rep_cluster_1448"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.287"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4050..5014
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.41"
                     /coverage="95.0"
                     /evalue="1.55e-177"
ORIGIN
        1 gattattata tgagcggaga aactgttatt tacaaaaatg aaatgaattt agttccatta
       61 agacgcttta ctgctactga aattaactta ttttttgcta tgtgtaataa gctcaaagaa
      121 caagatacta atacacttca tttgtctttt gacgaactca aagaattaag taattacagt
      181 ccagaaacac gtaacatcaa caggtttgta gatgatctcc aaagagttta cgataagatg
      241 ttagatatca gatatactat tagaactgaa acaaaaatta aaaaatttgt cttattctat
      301 aagtatgaaa tagatatatc ggaaaaatat ttagaaattg ctacttctcc tgatttaaaa
      361 tatatcttaa attcaattac gaataacttt actaaatttg aattaaaaga gatgacacac
      421 cttaaatcca cctattcaaa aaatatgttt agattactta aacaatataa acatactggc
      481 tatatgaaga ttaaaattga agactttaga gaacgattag atattccaaa atcttataga
      541 atgtctaata ttaaccaatt tgttttaaca ccgattatca aggagctctc tccaatattt
      601 aataacctca atatcaataa aatcaaagct aaaaaaggtc gtaaaattga gtggttagag
      661 tttacgtttg atgctgagaa acgcattcac agtaaacgac aacctcaaat gacggatgta
      721 ggtaagtcac gacaatatat cagtcgcgaa aaaacgccga aatggttaga agaacgcaca
      781 tatgaaaaaa caacacaaaa tgagtatgat ccacaattag agaaagaacg agaggcattt
      841 ttaaaacaac ttcaagttga ttgggaagaa tagaaaagtt agaaattgag acttatgtaa
      901 tatcatgata ttatataagt aacaagaatt tcgtttataa acgcaccaaa ccccgaacta
      961 ttcacagtag ttcggggttt ttgggtacat tgactgaatc gtctatgtta ttttttatca
     1021 cgtttatgga gccaataacc aaataacgta actagacaac cactgattgc cgttgtcatg
     1081 atgtgaacaa gaatttcatt cataaagcgc acctcccctc tacgtcaaat tgacgtctga
     1141 ggaatagacg acttcccaat tataacaaaa tgtctctaaa cttgtatgcc tgcatacaat
     1201 atacatgcat acatgataca cgtatactgt atgcaacact cagacgcttg agaactgaat
     1261 aaagatacaa aattttattt aatgaaaatg catgaaaacg cgtagttaag ggaatgtata
     1321 aaatatgaat aatccatgaa ttcacataaa agggacaaac gccgtcattt cagcgtttgt
     1381 cccttttgga ttggttaaca taataaagtg ttatgggtag ttgaattgta tatgttatgc
     1441 aatacgctaa cttaataaat attacccatc aatgattcaa taaaaagaca acaagaataa
     1501 tctttattgc ttaaatttaa agtaatttat ttatctaaat ttcttttttc atttattaag
     1561 ttttgaaact cttgttctaa ttcttccgaa ggttcaatac tcaatcgact gagtacttct
     1621 gtaatttttg tctcaagtaa aagtttttta tcttctttaa tattccttgt tggcttttca
     1681 gcttgtttaa attgttcata tgtgccatca aatattttta tttctttatt atcaattgtc
     1741 attattcgag tggctacttt ttcgataaat ttacgatcgt gagatacaaa gattatactg
     1801 ccattatatt cctttaacaa agattcaaac gcctctatag cttccatatc aagaaagttt
     1861 gttggttcat ctaataccaa cgtattaact tcacttaaga atactttagt tagtgctact
     1921 ttaactcgct ctccaccact taagacactt attggtttat aaacatcatc tctaaaaaaa
     1981 tgcattctag ctagaatagt tcgaataaga gtttcatttt gttgtgaaga agattgaacg
     2041 ttttctaaaa tactcttatc taattctaat gtatctattt tttggctaaa ataaccgatt
     2101 ttgacagatg gcgataatga aataccagga ttcccatgca caattttttt aataaacgtt
     2161 gtctttcctg taccattaga tccgataatt gccatcttgt ctcctccgcg aatactaaaa
     2221 ctttttgctt tccacaatac ccgtccttca attgtacccg agacatcttc accacgtatt
     2281 atagttctat tttttacact ttctaagttc actaaatcca ttttaagtgg aggaagttcg
     2341 tttctctttt cgacgctttc aagtttttct agtctggttt ctagagattt tacagttttt
     2401 cgtaacttct tttgcttacc tgcaaagtat ggctttgctc ctgttatttt gccttcagat
     2461 aaacttaagt ttttcggttt tttagttgct cgttgagctt tctgttcttt tatatttata
     2521 gctttttcca atcgtttctt ttctttttca tatttttcat attctaattc ttctcgatgt
     2581 ctttctaatt ctttttgttc aacatagtta ctataattcc ccttatattc agttattctc
     2641 ccctcgtcaa tttcccatat agtagtacac aagttatcta aaaaagcgcg atcatgtgaa
     2701 actataataa atgctccatg ccaatttttt aaatcctgtt ctaatttttc tatatagtta
     2761 ttatctaagt tagttgttgg ttcatctgct aatagcagtt ctggattttt atcaagcgtt
     2821 tgccgaatat agtttcgtgt tacttcacca ccacttttag ttgattctat gagcttcaat
     2881 tgaggaataa gttcacaatg tgaaaattgt tttacaatac cttcttcagg cacaattttt
     2941 ttatataata tgtgaagtaa cgttgttttt ccacttccat ttttaccaat taaaccaata
     3001 cgatcattct gataaatatt taggcgattt atgttcaaca ataaacgatc ttgaacataa
     3061 tgttttatat gaagtccctc taacattatt ttcatatttt ctcatcctct tataagtaat
     3121 tgaagacaat aaaagcctcc caatctattc agaataggag gcataaatct atatagatga
     3181 actctaacaa aatactttta gagccaacta cagaattttc atcctatcct gaaaagtaca
     3241 attgaaaaca acacaaatca aacgctgttt gtaaataaaa cactttcaat tgtttacttt
     3301 tgaaaatagg attagttaat catgtagttg gttcaccctt tcctttatcg tattaaattc
     3361 agcttaaaca aatagcaaat aaatgtcaat gttcaactca aattattttt ttaatttatt
     3421 tatattactt aatgaacagt ggctagcaat tagccactta ttttttcgca gaaaaaatcc
     3481 taaggggctt ggggatattc cccaacaagc aggcgtcgct accacgatag tggctagcaa
     3541 agccaatgct tgccaaacca ctatattact tgatgaaatt tagattatca tataatctaa
     3601 atttatatta agtaatatct ttcgtagaaa gcgtggtggg atgtatgagc gaacaaaacc
     3661 attttgtggc tagcgacgca actgttgggc aaaaccataa acccaaccgt aaggagccaa
     3721 aacaaattag ttttcgtgtg agcgaatccg aatatttaaa gttaaagcag tcagctgaaa
     3781 ctttaaatat gagtgtgcct gctttcgtta agaaaaaggc acagggcagt cgattggtcg
     3841 cacctaaatt tgataaagaa acgcgacaat cgattgccaa agatttaagt cgattagggg
     3901 cgaatgctaa tcaaattgcg aaatattgtc atcaacatca atatgacgca ccaaattata
     3961 gcgcattaga acgtaatatc agtgaattac gtgaaaggtt ggatgacata tggcaacaac
     4021 taaattaagt gcgacgaaat caacgtcacg tgccattaat tacgctgaaa aacgggctga
     4081 agaaaaaagt ggcttaaact gcgacataga ctatgctaaa agtagtttta aagccacacg
     4141 tgaactctat ggtaaaacag ggggcaatga aggccatgtc attattcagt cattcaaacc
     4201 aggcgaagta accccagaac aatgtaatca actaggctta gaattaactg aaaaattcgc
     4261 acctaatcat caagtcgctg tctatacaca caacgatacg gaccatgtac acaatcatat
     4321 tgtgattaat tcaattgatc ttgagacagg gaaaaaattt aacaacaata agcaagcctt
     4381 acgtgattta agagacttta atgacgacgt atgtcgtgaa catggcttaa gcgtgccaga
     4441 aaaagataca gcacgcttac gttatacaca aacagaaaaa gcgattgctg accctaatac
     4501 aaaatcaacc gctcaatatt catggaaaga tgacatacgt gaagcaatcg accaatcaac
     4561 agccacaaat atggacgaat ttaaagacca tttaagtcaa tacggcatta aaattgcacg
     4621 agttacacct aagagcatta cctatcgaca tctagctgaa gataaaaaag tccgtggccg
     4681 tcgattaggt gaagattata acaaaggggg catagaagat ggctttgaaa gacaaataca
     4741 acgacaacag caagaacgag aagatgacaa cgacatccag ccaaaacgac caacttcaaa
     4801 ccgtgatgaa tcaactgaac gagatacagg cgtcactcaa gcagattggg aacaattcgc
     4861 acaagacaca aatgagcttg agcgtagccg acaagctgca gaatcagcaa gacttgctca
     4921 tgaaaaggct cgaagagatc gagaagagag agcacgagag aaacaagcgt cacgaacaat
     4981 tattgacaac gatcgagacc acggcctcga actttaatca agcgactgaa atcacacaaa
     5041 agcgtttcat cagtgtagcg aaacactata tcgaacgcat taataacgat aatttaaaac
     5101 aagattttca aacggcgata caagaagaat tggaagatgt gaaatcacag acacaaaatt
     5161 tagttaaaca gtttcaaaat catcaatcag acttacaaaa agcacatgat gattataaaa
     5221 aaacgcttga tgagcgtatc gaaacaaacg aaatagcaat aaaacaatat gatcaagcat
     5281 tcaatcgttt aactaaaggg attacagcca tgtttttcat cgttgcctta gtaatgatta
     5341 cttttgtagt cttaagtcca ttaggcgacc tattaggcgt acaacatttt tatgattgga
     5401 ttaaccacgt cttaaaaaca ggtcattcag catggcgtta tctaattgtg acgctttatc
     5461 ttgcccctta catattattt agtttgctta tatatgccat tttaaaggcg tatgagcgac
     5521 tttaacctta gtcagtatta aaaacctata acttatcatg aaaaacgctg catacggttt
     5581 aaattagttt agaaatggaa ttaaacattt tttataagga gatccgaaat gcttatattt
     5641 ttgtttattt taatgagtgg acttttaagt tattacttga ttaatcgttc aaacaaaaag
     5701 aaatgacgct attatgccga gaaaatttat tgtgcattga gaagaaccct taataaaccc
     5761 taaaactgtt tcgacgaatg tcggtatagc gtgagctata aaaccgacga gtcgacagtt
     5821 ttagggttta ttaagggttc agaggctcaa tgtcaataaa gcaattggaa taatgcaata
     5881 agtaaatgat ttttattaat caaaaccaat catattttat gattggtttc ctttttgttt
     5941 ttttgaattt tgaggggcaa attgccccga actttttaat atctttttat aaatcttttt
     6001 aaaacctttt taggaggtgc tttagaccta gagtgccaag gattatagaa tgttaaaggc
     6061 atagttttgc cacattaaag gcatagtttt gccacattaa aggcatagtt ttgccacatt
     6121 aaaggcatag ttttgccaca ttaaaggcat agtttttttg ttgttatgcc tattattcta
     6181 tgttactgta taaacaagct ttaatacata atattataga atttaaaggc atagtttttt
     6241 tgttgttatg cctttattaa aaggag
//
