LOCUS       NZ_CP069469             9269 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1249 plasmid unnamed10.
ACCESSION   NZ_CP069469
VERSION     NZ_CP069469.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357391
            Assembly: GCF_016889125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 864.31x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 04:58:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,157
            CDSs (total)                      :: 5,036
            Genes (coding)                    :: 4,746
            CDSs (with protein)               :: 4,746
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 290
            CDSs (without protein)            :: 290
            Pseudo Genes (ambiguous residues) :: 0 of 290
            Pseudo Genes (frameshifted)       :: 76 of 290
            Pseudo Genes (incomplete)         :: 208 of 290
            Pseudo Genes (internal stop)      :: 41 of 290
            Pseudo Genes (multiple problems)  :: 34 of 290
            Pseudo Genes (short protein)      :: 4 of 290
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069469.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1249"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1249"
                     /db_xref="taxon:562"
                     /plasmid="unnamed10"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(486..1289)
                     /locus_tag="I6J79_RS25385"
                     /old_locus_tag="I6J79_25385"
     CDS             complement(486..1289)
                     /locus_tag="I6J79_RS25385"
                     /old_locus_tag="I6J79_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204147842.1"
                     /translation="MGIFSQIGSALLNCISKPVDALCEWAKEPLKDREYRREEASKESE
                     HKRNMDVLTAKKRVEHELRMSELNAEYELREKEKTLEVDLEVRRVREIEKAVAEIQEWR
                     KSQELERMERTTAAIARYKEQLTKLNIEVINSIGNMQLDLKERAQKFVYEKAVQYKRLQ
                     DEATEEAMNDLIRIEEKFGDNERAKDILIRAVDTKMGNVIDTSTRFLEELNRDIVSLNQ
                     SIDRLTNQGQKFIESHLERFHIVDATSSLYITSDEKERLLLKNIN"
                     /name="hypothetical protein"
     gene            complement(1276..1815)
                     /locus_tag="I6J79_RS25390"
                     /old_locus_tag="I6J79_25390"
     CDS             complement(1276..1815)
                     /locus_tag="I6J79_RS25390"
                     /old_locus_tag="I6J79_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204147843.1"
                     /translation="MAGGRHINSLLYVLVAILERKVNDFKLSSSELPEILAALREAREC
                     LVSRSINEDPQLYSALFLYEKNEGLLSLSFEREYSIFINSNLECILRKVADIKDKKQGK
                     LQKIMNELVRFKYSSSDSCADLLFLSGKLEQIRKIYEVFDRDRNRLEFLNYMMKKERVI
                     FEREFERLLGEKNGDF"
                     /name="hypothetical protein"
     gene            complement(1818..2429)
                     /locus_tag="I6J79_RS25395"
                     /old_locus_tag="I6J79_25395"
     CDS             complement(1818..2429)
                     /locus_tag="I6J79_RS25395"
                     /old_locus_tag="I6J79_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="WP_251301786.1"
                     /translation="MTDKTTDIKMALENMIRFRDTIERLGGAMSKELDVETKGKRTYSM
                     NNDMSDLYNVISRAVEASGADHSINEKLTNVLKRELVDYVSIAHLKTKLSVLYEFEKNY
                     LQLIAEYKEEIKFASSLQEDLRKERAKFFSETLKEVHQTLSESQVDNEVASKWIKELVG
                     SYTKSLDLSGGLVEEHTLDTIACIRAEAKLNKPSIEPGNN"
                     /name="nickel transporter"
     gene            complement(2762..2995)
                     /locus_tag="I6J79_RS25400"
                     /old_locus_tag="I6J79_25400"
     CDS             complement(2762..2995)
                     /locus_tag="I6J79_RS25400"
                     /old_locus_tag="I6J79_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3002..3520)
                     /locus_tag="I6J79_RS25405"
                     /old_locus_tag="I6J79_25405"
     CDS             complement(3002..3520)
                     /locus_tag="I6J79_RS25405"
                     /old_locus_tag="I6J79_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_204147844.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3558..3989)
                     /locus_tag="I6J79_RS25830"
                     /pseudo=""
     CDS             complement(<3558..3989)
                     /locus_tag="I6J79_RS25830"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4198..4521)
                     /locus_tag="I6J79_RS25415"
                     /old_locus_tag="I6J79_25415"
     CDS             complement(4198..4521)
                     /locus_tag="I6J79_RS25415"
                     /old_locus_tag="I6J79_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4586..4777
                     /locus_tag="I6J79_RS25420"
                     /old_locus_tag="I6J79_25420"
     CDS             4586..4777
                     /locus_tag="I6J79_RS25420"
                     /old_locus_tag="I6J79_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5980..6846)
                     /locus_tag="I6J79_RS25425"
                     /old_locus_tag="I6J79_25425"
     CDS             complement(5980..6846)
                     /locus_tag="I6J79_RS25425"
                     /old_locus_tag="I6J79_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(6848..7900)
                     /locus_tag="I6J79_RS25430"
                     /old_locus_tag="I6J79_25430"
     CDS             complement(6848..7900)
                     /locus_tag="I6J79_RS25430"
                     /old_locus_tag="I6J79_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(8381..8614)
                     /locus_tag="I6J79_RS25435"
                     /old_locus_tag="I6J79_25435"
     CDS             complement(8381..8614)
                     /locus_tag="I6J79_RS25435"
                     /old_locus_tag="I6J79_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(8621..9151)
                     /locus_tag="I6J79_RS25440"
                     /old_locus_tag="I6J79_25440"
     CDS             complement(8621..9151)
                     /locus_tag="I6J79_RS25440"
                     /old_locus_tag="I6J79_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     Typing          complement(4872..4953)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(2..95)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="87.0"
                     /evalue="4.21e-42"
     Typing          complement(5410..5597)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(2659..4208)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.611"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8278..9269)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
       61 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgccttaa cgccactggc
      121 agcagccatt ggtaactgat ttagaggagt ttgtcttgaa gttatgtgcc tgttaaggct
      181 aaactgaaag aacagatttt ggtgactgcg ctcctccaag ccagtgacct tcggaagaaa
      241 gagttggtag ctctagattc cggcaaacaa accaccgttg gtagcggtgg tttttttgtt
      301 tacaggcagc agattacgcg cagaaaaaaa ggatcacaag aggatccttg aatcttttca
      361 ctcccactcc taggtcatag ccatagcgct cagttttcaa ccacaagaca aaaactaatg
      421 attagcaacc gcaagaacaa acaatgcgac cattaaaaat aatgatcgct attactcaca
      481 aaatactaat taatattttt cagcaacaat cgttctttct catcacttgt aatatataaa
      541 gaagatgtcg catcaacaat atgaaaacgt tccaaatgac tttcaatgaa tttttgtcct
      601 tgattagtca gcctatcaat actttggttt aaactaacaa tatctctatt taattcttcc
      661 aaaaaccgag ttgacgtatc aataacattc cccatcttag tatcaactgc acgaattaaa
      721 atatctttag ctctttcatt atcaccaaat ttttcctcaa tccttataag atcattcata
      781 gcctcttcag ttgcttcatc ttgcaggcgc ttatattgta cagctttctc atagacaaat
      841 ttctgtgcgc gctcctttaa atcaagctgc atgttcccaa tagagttaat cacttctata
      901 tttaatttcg ttaattgctc cttgtaacgt gcaatagctg ctgttgttcg ctccatcctc
      961 tcaagttcct gtgactttct ccattcctgg atctcagcta ctgctttttc tatttcgcga
     1021 acgcgacgaa cctctagatc aacttcaaga gttttctctt tttctctcag ctcatattca
     1081 gcattcaatt cactcattct gagttcatgt tcaacacgtt tcttggccgt caagacatcc
     1141 atgttgcgct tatgttcaga ctcctttgaa gcctcctctc ttctatattc cctatctttc
     1201 aatggttctt tagcccattc acagagtgca tcaacaggct tactaataca attaagcaaa
     1261 gcgctaccaa tctgactaaa aatccccatt tttttcacct aaaagccgtt caaactctct
     1321 ttcaaaaata acacgttctt tcttcatcat atagttaaga aattcaagtc tatttctatc
     1381 cctatcaaag acctcatata ttttacgtat ctgttcaagt ttaccactta aaaacaaaag
     1441 atccgcacaa gaatcgctac ttgaatattt aaacctcaca agttcattca ttattttttg
     1501 cagtttaccc tgctttttat cttttatatc agcaactttc ctcaatatgc actctaaatt
     1561 ggagttaata aaaatagagt attccctctc aaatgaaaga cttagcaacc cttcattttt
     1621 ttcatataaa aacaatgccg aatataactg aggatcttca ttgatgctgc gcgatacaag
     1681 gcattccctt gcctctcgta aagcagccaa tatctcagga agttccgacg aagacaattt
     1741 aaagtcattc actttacgtt ctaatatagc cacaagaaca taaagcaaac tatttatatg
     1801 tctacccccg gccataatta gttattgcct ggctcaatag atggtttatt taatttagcc
     1861 tcagcgcgaa tacaagcaat tgtatctaat gtatgctctt caacaaggcc tccgcttaga
     1921 tccaagcttt tggtataact accaacaagt tctttaatcc attttgatgc cacttcatta
     1981 tcaacttgag attcacttag agtttgatga acctctttta atgtctcaga aaagaattta
     2041 gcacgttctt tacgtaaatc ctcttgcaaa gaggaagcaa attttatttc ttccttatat
     2101 tctgcgataa gctgcaaata attcttttca aactcataca atacagacaa tttggttttt
     2161 agatgcgcaa tgctgacata atcaactaat tctcttttca agacatttgt caatttttca
     2221 ttaattgaat gatcggcacc agacgcttca acagcccgtg atattacatt atataaatca
     2281 ctcatatcgt tattcataga ataagtacgt tttcctttgg tctcaacatc caactctttc
     2341 gacatagccc cccccaatct ctctatagtg tcacgaaatc tgatcatgtt ttctaaagcc
     2401 attttaatat cggtcgtttt atctgtcatg acttaggcct aactcatcta tttaccataa
     2461 cgtcgcatta cgcgcactgc ttaaacgcgt tcagcgcgat cacggcagca gacaggtaaa
     2521 aatggcaaca aaccacccga aaaacagccg cgatcgcgcc tgataaattt taaccgtatg
     2581 aatacctatg caaccagatg gtacaggcca cattaccccc tcttaatcca ctgaagctgc
     2641 catttttcat ggtttcacca tcccagcgaa gggccatcca gcgtgcgttc ctgtatttcc
     2701 ggctgacgct cccgttccag ggataacgca tgttcgcgct cctgtatcag ccgttcctct
     2761 cttatctcca gttctcgctg tataactggc tcaagcgctc tgtctgctcg ctcaagtgct
     2821 gcacctgctg actcaactgc atgacccgct cgttcagcat cgcgttgtcc cgttgcgtaa
     2881 gcgaaaacat cttctgcaat tccacgaagg cgctctccca ttcgctcagc cgctgcatat
     2941 agtcctgttg cagctgctct aaggcgttca gcaaatgtgt ttccagctct gtcactctgt
     3001 gtcactcctt cagatgtacc cattccttcc cctgaaagga aatcacctct gctgacttcc
     3061 cgtaaggaag gaccagaaat ttcctgttcc cgtcctgcac aaactccacg ccccatgtct
     3121 tcgcgttcag tttctgcaat gtctcttcct gcttcctgat atcttccagg ttcgcctgta
     3181 tcctcccgcc aagataccag agcgtcccgc cactcgcggt aaacaggaga aagactatcc
     3241 ccagtaacat catgcccgta ttccctgcca gctttaacac gtccctcctg tgctgcctca
     3301 tcgcctcttt caccccttcc cggtgttttt ccagcgattc ctctgtcgag gcggtgaaca
     3361 gggctatagc gtctctgatt ttcgtctcgt ttgatgtcac agcctcgctt acagattcgc
     3421 cgagcctcct gaactcgttg ttcagcattt tctctgtaga ttcggctctc tctttcagct
     3481 ttttctcgaa ctccgcgccc gtctgtaaaa gattgctcat aaaacgctcc tttcagtctg
     3541 atattcctcc cgccgttcgg gtctgcaatg ctgatactgc ttcgcgtcac cctgaccact
     3601 tcaagccccg cctctgtgag cgcctgaatc acatcctgac ggccttttat ctctccggca
     3661 tggtaaagcg cgtctatccc gcgtgtgacg ccctcagcaa gcgcctgttt cgtttccggc
     3721 aggttatcag gaagggtcag cgtccggcgg ttctccgggg cgttggggtc atgcagaccg
     3781 taatggtgat tcaccagcgt ctgccaggca tcaattctcg gcctgtctgc ccggtcgtag
     3841 tacggctgga ggcgttttcc ggtctgtagc tccatgttcg gaatgacaaa attcagctca
     3901 agccgtccct tgtcctggtg ctccacccac aggatgctgt actgattttt ctcgagaccg
     3961 ggcatcagta cacgctcaaa gctcgccatc actttttcac gccctccagg cggcagctcc
     4021 ttctccgcga acgacagaac acctgacgtg tatttcttcg caaatggcgt ggcatcgatg
     4081 agttcccgga cctcttccgg attcccctga agcaccgttg ccccttcccg gttacgctcc
     4141 cttcccagca ggtaatcaac cggaccactg ccaccacctt ttcccctggc atgaaactta
     4201 actatcatcc cgcgccccct gttccctgac cgccagacgc agccggcgca gctcatcccc
     4261 gatggccatc agtgcggcca ccacctgaac ccggtcaccg gaagaccact gcccgctgtt
     4321 caccttcctc gctgtctggt taaggttatt cccgatggcg gccagctgac gcagtaacgg
     4381 cggtgccagt gtcggcagct tcccggaacg ggcaaccggc tcacccaggc agacccgccg
     4441 catccatacc gccagttgtt taccctcaca gcgttccagt aaccgggcat gttcatcatc
     4501 agtaacccgt atcgtgagca tcctctcgcg tttcatcggt atcattaccc ccatgaacag
     4561 aaccccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc
     4621 ccgcttcatc agaggccaga cattaacgct gctggagaag ctcaacgaac tggacgcgga
     4681 tgaacaggca gacatctgtg aatcgcttca cgaccacgcc gatgagcttt accgcagttg
     4741 cctcgcacgc ttcggggatg acggtgaaaa ccgctgacac atgcagctcc cggagacggt
     4801 cacagcttgt ctgtgagcgg atgccgggag cagacaagcc cgccagggcg cgtcaggccg
     4861 ttgacctttg gggtgtcggg gcgaagccct gacccagtca cgtagcgata gcggagtgta
     4921 tactggctta accattcggc atcagtgcgg attgtatgaa aagtgcgcca tgtccggtgt
     4981 gaaatgccgc acagatgcgt aaggagaaaa tgccagttca ggcgctcttc cgcttcctcg
     5041 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggactctcc tttctcaaaa
     5101 acgctaaccc tgttatccac agaacccggg gataaaccag gaaagaacat gtgcgcaaaa
     5161 aacgaaaccc cgggaaaggc cgcgccggag gcgctttttc cataggctcc gcccccctga
     5221 cggacttcac aaaacagaca ctttttcggt gcatctgtgg gagccttgcg gctcaaccat
     5281 gaaatccgcc agcggtggcg aaacccgacg tggcttaaag atcatcaccg tttccggtga
     5341 atcgctccct cctgcgcccc cacgttcccg ccatatcgat taccggatac cttttccgcc
     5401 tttctccctt acgggacacg tggcgctttc tcatagctca cgtcttctgg tatctcgggg
     5461 cgacttaggc agttcgctcc gggctgggct gtatgcagga accccccgtt cagcccgact
     5521 gctgcgcctt ctccggtaac tgttctcttg ggtccaaccc ggaaaagaca ccacaaagcg
     5581 ccactggcag cagccattgg taaaagtcat gtgcgagata tttagagagt acttgaagtg
     5641 gctgccggag tgcggctaca ctagaagaac aattttggtg actcgtctcg cataagacag
     5701 ttacctggtt aagcagtctg caagctgact taaccttcga tcaaaccgcc ctgcaaggcg
     5761 gttttttcgt ttacaggcag cagattacgc gcagaaaaaa aggatctcaa gaggatcctt
     5821 tgatcttttt gttacaaacg tctctatttg actcccctga gagcgtttta gcgcgcctct
     5881 gacccccttg gttacattcc gacaccagaa acgctcccgt tttttacgtg catttggcac
     5941 ggtatcgatg gaggccgggg ggcaatcctg aacagaatgt tactcctctg agtattttct
     6001 gttaagaaaa tatacactta gcacagttac aataaatcct accccctgta agaatgtgaa
     6061 ttgttcatcc aaaaacaacc atgacaaaaa taatgcaact agcggaataa agtacaacat
     6121 tatccctgcc aaatgaactg ggagccaacg caaggagttc aagtaaaaac aaaaagctaa
     6181 aaaatattgt aatattccag atgaaaaaac aataatcacg gattccaacg gaagataaat
     6241 tgatgattta gatataaaaa taaagagcga tgacagtata agggagaatg tcaactgtcc
     6301 gctcaataat gtgataggct caaacgattt aataaggcga gacgatataa caacataaaa
     6361 tgctgccgat aacacaccgg caacaacaag acaatatcca acaatattat tcgatccatt
     6421 actgatgtct ggcaaggcta ccaaaatact gccaacaatt gcacctataa ggaacaatat
     6481 aaaatattgc ggggaattaa ttttaactga gcaaataaga acagacaaca ccaatataaa
     6541 cactgattca agtgaaaaaa taattgatgc gcttcctgct gaaataaaat aaagtccgta
     6601 gagtgtaaaa gtatacgcta agaatggttc aaaaacgcct gtcgcataag caagtatcgc
     6661 ttttgagttt aatgggagtt tttttctttt tataaaaaga actatccaag atagtattac
     6721 tgcactacaa atctgccaga aaaacacccc tccggcatct agttcttcaa ctcctttttt
     6781 gctcattacg atagctgccc cccatgagaa cgctgctaaa aaagcaaaaa gcattccttt
     6841 aatcatatta atcatcctct ttctcataaa ggggtttgtt ttcaaatttc cttggttgaa
     6901 aggagttaaa aaacatagat gcatcatcta caggaatacc aataaactct ccataataag
     6961 gatcatctat aggaaactct tctaatgaaa cataaaacaa tacattccag ttgaagatca
     7021 ttggatcact attttctatt tcatggagca atctttcatc cttagttaat tctcctggaa
     7081 ttaatacatc agtatgataa aaaccgctgt aataagaact attaccggtg tgcactgggc
     7141 atccaaaacg atccaaatta gattctgaat gccatgttgt agaaatccct tccggatgtg
     7201 aatacccacc cttaccaaca gactgaatag tcatgttacg aatatccgct aacgcttgtt
     7261 cttgatcttt atagaaacct ttttctatcg ccagcctcat aaaatgcccc ggggcattta
     7321 cagaaataag tattccccct ggtattcttc tatcatgatt tcttgttcta tgagcagaag
     7381 agtaaaaaac attaacacca gccttatata tttttagccc atctttatcc tgaaatggta
     7441 cgctttcagt ataaataaca tcctctttta ttggatagca ctctggcata tgcagcgaac
     7501 aaatatattt ttgtatgtcc actaactctt ctgatggacg agcaaaggca agttgtggat
     7561 gaataaaata aatgagatgc gcactgacct ctcctcttct tgcgcgttct ttaaaacgaa
     7621 ctctttcatt acgcaaaaaa tcaaacaaat gaccatcatc attatatagt tgggggctat
     7681 ttacaataga taaatggaaa tcaagccctt taattttctt gcttgccaat ttaccaaata
     7741 cacagggctg attgcctgac acccattttc ttagtaaatc ttcaatttta tctttagaag
     7801 tttcgttaaa gataaatgaa tgaatattat caatatcttt tccaaatgaa aaactggatg
     7861 ataattgtgg tagtttattt atacgttgtt gaagattcat gaaagctccc acaagacacc
     7921 tcatatattt ttttatatac tgaaactaca ttatatttat taccggcgta aaacgaaata
     7981 atttattatc gttaccgata ttttaaaaaa atcattattt gcacagtgtt cgagttgtta
     8041 gtatcactcc ttcctgaaaa tccgctccat ttaccataac gtcgcattac gcgcaccgcc
     8101 aaaacgcgtt cggtgcgatc acggcagcag acaggtaaaa atggcaacaa accacccgaa
     8161 aaactgccgc gatcgcacct gataaatttt aaccgcatga atacctatgc aaccagatgc
     8221 tacaggccac attagcccgc cttaatccac tgaacacgtc atttttcatg gtttcaccat
     8281 cccagcgaag ggccatccag tgtccgctcc tggatttccg gctgacgctc ccgctccaga
     8341 gataactcat gttcgcgttc ctgcatcagc cgttcatccc ttatctcctg ttctcgctgt
     8401 ataactggct caagcgttct gtccgctcgc tcaagtgctg cacctgctga ctcaactgca
     8461 tgactcgccc gttcagcatc gcgttgtccc gttgcgtaag cgaaaacatt ttctgcaatt
     8521 ccacgaaggc gttctcccat tcgttcagcc gctgcatata gtcctgttgc agttgctcta
     8581 atgcgttcag caaatgtctt tccagctccg tcatgctctg tcactcctgc accagtctca
     8641 ctgcgttcag tttgccgttg tccttcgtcc agttcgtttc ggctttcatc cccttcggca
     8701 acaccagaaa acgtccgttg ctgtcctcgt gataacgcac gccccatgtc ttcgcgttca
     8761 gcttctccag actctctttc tgctcgcgga gaatattcat attcgcgttt atcttcatgc
     8821 cctggtacca cagcagactc ccggtcgtgg caaacatcag tactgagacg ataatcagcc
     8881 acagccagcg tttcacgctc attttcagta cggttttcca gtgcgcgtcc atcgtatcga
     8941 cggtcgtttt ctgatacctc tccagcagtt ctttcagggc tgttgtatgg tcgttgatag
     9001 cgttcctgat taaagattcg tttgaggtca gttccctgtt tacagattct tcgagcttcc
     9061 tgaactcgtc gttcagcatt ttctctgtag attcggctct ctctttcagc tttttctcga
     9121 actccgcgcc cgtctgtaaa agattgctca taaaacgctc ctttcagtct gatattcctc
     9181 ccgccgttcg ggtctgcaat gctgatactg cttcgcgtca ccctgaccac ttcaagcccc
     9241 gcctctgtga gcgcctgaat cacatcctg
//
