LOCUS       NZ_CP069468            11379 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1249 plasmid unnamed9.
ACCESSION   NZ_CP069468
VERSION     NZ_CP069468.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357391
            Assembly: GCF_016889125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 864.31x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 04:58:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,157
            CDSs (total)                      :: 5,036
            Genes (coding)                    :: 4,746
            CDSs (with protein)               :: 4,746
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 290
            CDSs (without protein)            :: 290
            Pseudo Genes (ambiguous residues) :: 0 of 290
            Pseudo Genes (frameshifted)       :: 76 of 290
            Pseudo Genes (incomplete)         :: 208 of 290
            Pseudo Genes (internal stop)      :: 41 of 290
            Pseudo Genes (multiple problems)  :: 34 of 290
            Pseudo Genes (short protein)      :: 4 of 290
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069468.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1249"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1249"
                     /db_xref="taxon:562"
                     /plasmid="unnamed9"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            503..1051
                     /locus_tag="I6J79_RS25315"
                     /old_locus_tag="I6J79_25315"
     CDS             503..1051
                     /locus_tag="I6J79_RS25315"
                     /old_locus_tag="I6J79_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004744690.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_077070009.1"
                     /translation="MAILRINGNVKIGHIYECNFGMFKKDEAPQAGQAQLVTKDKNEAA
                     TDDYNYRIPNEMIKKRPIVVIGKHKGQYIVVPISSTKETDKKPAKTPENVGFHILLQPG
                     DMPVTARYVEEKERWAKSNLLMTIDGSRLTDIYDKGANQFVAAHKISDDTLLKIRHGVI
                     ISIGLRDILPQQPVEAPAE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(1230..1628)
                     /locus_tag="I6J79_RS25320"
                     /old_locus_tag="I6J79_25320"
     CDS             complement(1230..1628)
                     /locus_tag="I6J79_RS25320"
                     /old_locus_tag="I6J79_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_077070008.1"
                     /translation="MLTGLNHITIAVSDVSNSFNFYVNTLGFSPRARWSRGAYLSLGEL
                     WLCLSADVPIPAQDYTHYAFSIPSHKFDDFVEKLKSAGLVEWKVNKSEGRSVYFLDLDG
                     HKLEVHDGDLDSRLRECMIHPYEKMEFF"
                     /name="VOC family protein"
     gene            complement(1983..2192)
                     /locus_tag="I6J79_RS25325"
                     /old_locus_tag="I6J79_25325"
     CDS             complement(1983..2192)
                     /locus_tag="I6J79_RS25325"
                     /old_locus_tag="I6J79_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_141021236.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTDLSRQVQSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2196..2681)
                     /locus_tag="I6J79_RS25330"
                     /old_locus_tag="I6J79_25330"
     CDS             complement(2196..2681)
                     /locus_tag="I6J79_RS25330"
                     /old_locus_tag="I6J79_25330"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_141021235.1"
                     /translation="MSSLLTLAKDLEQKSKAQQQSTGEMLKVAFSEHEQSVRAELSESE
                     KRISAAILDHDRKLFSAMNQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQSQQIL
                     DNYMTIREQKNTEAILSERNSGLQLSTCGEQRRHCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2716..3150)
                     /locus_tag="I6J79_RS25820"
                     /pseudo=""
     CDS             complement(<2716..3150)
                     /locus_tag="I6J79_RS25820"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3359..3682)
                     /locus_tag="I6J79_RS25340"
                     /old_locus_tag="I6J79_25340"
     CDS             complement(3359..3682)
                     /locus_tag="I6J79_RS25340"
                     /old_locus_tag="I6J79_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_172902175.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLATWMRQTCLDEKPARTGKLPSI
                     SPALLRQFAGMGNNLNQIARQVNAGAGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAE
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4864..5076)
                     /locus_tag="I6J79_RS25345"
                     /old_locus_tag="I6J79_25345"
     CDS             complement(4864..5076)
                     /locus_tag="I6J79_RS25345"
                     /old_locus_tag="I6J79_25345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080111754.1"
                     /translation="MGDFERTFGAGADFDRIIDENNSEYFREDRRKTKNSKKNFSSFQD
                     ALVWAKANPGKTIIRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(5331..5858)
                     /locus_tag="I6J79_RS25350"
                     /old_locus_tag="I6J79_25350"
     CDS             complement(5331..5858)
                     /locus_tag="I6J79_RS25350"
                     /old_locus_tag="I6J79_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246513.1"
                     /translation="MKCFWACSVSVVLAMSFTVAAAENNDNVTYTKPSAGRDLYLTCKG
                     YQVKGASVINVEKPSYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMH
                     EHWSLVHVGGRASVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIY
                     TRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            complement(5987..6202)
                     /locus_tag="I6J79_RS25355"
                     /old_locus_tag="I6J79_25355"
     CDS             complement(5987..6202)
                     /locus_tag="I6J79_RS25355"
                     /old_locus_tag="I6J79_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6206..6691)
                     /locus_tag="I6J79_RS25360"
                     /old_locus_tag="I6J79_25360"
     CDS             complement(6206..6691)
                     /locus_tag="I6J79_RS25360"
                     /old_locus_tag="I6J79_25360"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<6726..7160)
                     /locus_tag="I6J79_RS25825"
                     /pseudo=""
     CDS             complement(<6726..7160)
                     /locus_tag="I6J79_RS25825"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7369..7692)
                     /locus_tag="I6J79_RS25370"
                     /old_locus_tag="I6J79_25370"
     CDS             complement(7369..7692)
                     /locus_tag="I6J79_RS25370"
                     /old_locus_tag="I6J79_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            <8708..8863
                     /locus_tag="I6J79_RS25375"
                     /old_locus_tag="I6J79_25375"
                     /pseudo=""
     CDS             <8708..8863
                     /locus_tag="I6J79_RS25375"
                     /old_locus_tag="I6J79_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            8957..9601
                     /locus_tag="I6J79_RS25380"
                     /old_locus_tag="I6J79_25380"
     CDS             8957..9601
                     /locus_tag="I6J79_RS25380"
                     /old_locus_tag="I6J79_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     ARG             8958..9598
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1231..1628)
                     /name="FosA8"
                     /gene="FosA8"
                     /gene_name="fosfomycin thiol transferase"
                     /tool="rgi"
                     /drug_class="phosphonic acid antibiotic"
                     /identity="52.94"
                     /coverage="93.62"
     Typing          complement(3792..3970)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.153"
                     /coverage="100.0"
                     /evalue="3.53e-41"
     Typing          complement(7806..7983)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="100.0"
                     /evalue="4.5e-50"
     Typing          complement(10877..11050)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          2..379
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.412"
                     /coverage="99.0"
                     /evalue="8.73e-86"
     Typing          3923..4435
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.868"
                     /coverage="99.0"
                     /evalue="5.84e-177"
     Typing          7930..8453
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.057"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          11281..11379
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.0"
                     /coverage="99.0"
                     /evalue="2.07e-34"
     Typing          complement(1874..3369)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.976"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5896..7379)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.717"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
       61 cccccgttca gtccgactgc tgcaccttat ccggtaacta tcatcttgag tccaacccgg
      121 aaaggtatgc aaaagcgcca ctggcagcag ccattggtag agggtgtaca agagattact
      181 gatgcagagt tcttgaagtc agagccaaac tacggctaaa ctgaaaggaa gaatttggtg
      241 gcagcggtct tgtacaccct gttaccacgg ttcagaagtt ccccaactta ctgaaccttc
      301 gaaaaaccac ctccccaggt ggttttttcg tttctgggac aagagactac gacggaagat
      361 gatggatctc aagaagatcc ggctatcgcc attattgaat gcatgtaaga tttatctttt
      421 tattaaggtg aacataggta ccacactaat aaaaagacat atttctaaat taaatcaata
      481 aattaaaaag caggcatatc acatggcaat cttgcgcata aatggaaacg taaaaatagg
      541 ccacatttac gaatgtaatt tcggcatgtt caaaaaagat gaagccccac aagcaggaca
      601 agctcagctg gtaacgaagg ataaaaacga ggctgctact gacgactaca actatcggat
      661 accgaacgag atgataaaaa agcgtccgat agtagttata ggaaagcata aaggtcagta
      721 cattgtggtt cctattagta gtacaaaaga aactgataaa aaacctgcca aaaccccaga
      781 aaatgtcggt tttcacattc ttctgcaacc tggcgatatg ccggtcactg caagatatgt
      841 cgaggaaaaa gagcggtggg caaaaagtaa tttattaatg actatagatg gcagccgcct
      901 gaccgatatt tatgataaag gagcaaatca atttgtagcg gcacataaaa tatctgacga
      961 tacgctgcta aaaattaggc acggtgtaat tatcagcatt ggccttcgag acatactgcc
     1021 tcagcaaccg gtagaagcgc cagcagagta aaatctggtt gctcgttttt gagtaataca
     1081 gtactattat cctgtagccg caaggcaagt tagatttttt agccgcaagg caagttttag
     1141 aaaaggccca gtcaatgact gggccttttc actttcatca atcctaaaaa attgaacata
     1201 acatccgtgt tgcgccccac aggtaagtat tagaaaaact ccattttttc atatggatga
     1261 atcatacact ctcttagtcg gctatctaaa tccccatcat gcacttccag cttgtgtcca
     1321 tctagatcca aaaagtaaac agaccttccc tcactcttgt taactttcca ttcgactaat
     1381 ccagccgatt tcagcttctc aacaaaatca tcgaatttat gggaagggat gctaaatgcg
     1441 taatgtgtat agtcctgcgc aggtatgggc acatcagcag aaaggcatag ccaaagctca
     1501 cccagggaaa gataagcccc acgactccat cttgctctcg gtgagaagcc aagcgtgttt
     1561 acgtaaaaat tgaagctgtt tgatacatca ctaacagcaa tggttatatg attcagacct
     1621 gtcagcattt cctgacctcg ttatctatgt gccattcact cttatttacc ataacacccc
     1681 ttacgcgcac cgctcaaacg gcctcagagc gatcctgagg ccaaagtcat aaaacactcc
     1741 gtcttacccc ctgaaaaccg cccaggatcg ctcctgtgat ttcacaggcg atgaatcgtt
     1801 atgcacccga acgactgacg catgatttca gaacaaatcc gccctgaaat cgctgttttc
     1861 tccggtttat cacagcgacg gtccctcata cgtctgctga cgctgagagc gttcaagctc
     1921 caactccttc tcgtgctgct ccatgcgctc ctgctcccga agagcctcaa tactgttcag
     1981 ctttacgaca accggcgtag ctgctcgctc aaactctgca cctgcctgct caagtccgtg
     2041 acgcgctcgc tcaacgccgc gttctcccgt tgcataagac cagacatcgt ccgccattcc
     2101 gcgaaggcgt tctcccactc gtccagcctt ttcgagtagt cctgctgtag ctgctctaat
     2161 gcgctcaaca actgtttttc cagctctgtc atgtgctatt tccccgccag tatcatccag
     2221 ctcgaatcct ctccgaaccg tcccgcttcc gggttcaccc tcacgcagtg gcgtctctgc
     2281 tctccacagg tcgagagttg caggccgctg ttcctctctg agagtatggc ctccgtgttc
     2341 ttctgctccc ggatggtcat gtaattgtcg agtatctgct gactctgcca ccacagaatg
     2401 cctgcgctcg aggctatcag gagcgcggaa acgaggacga tggtcagcca cgtctggctg
     2461 accatgcgca gcatcccttt cgtacgctga ttcatggctg agaacagttt ccggtcgtgg
     2521 tcgaggatgg cggcgctgat tctcttctcg ctttcgctca attccgctct gacagattgc
     2581 tcgtgctcgc tgaatgcgac tttcagcatc tcgccggtac tctgctgctg cgctttcgat
     2641 ttctgctcta agtccttcgc cagcgttaaa agactgctca tagatggctc ccttcagtcg
     2701 gatatttcgc cccccgtctg ggtcggcaat gctgatactg ctttttgtgg tgcgtaccac
     2761 ctcaaaacct gcgttttcca gcgtctcagt aacgtcctga cgcgttttca gctccccgga
     2821 cgaagctaag gacagtaaac ctcgcgtaat cgcttgagcg gcttcctgct tcgcttccgg
     2881 caacgcagat ggcgtgacca gcgctcgccg attctccgga gcattcgggt cgtgcagccc
     2941 cagcctgccg ttcacgatag tctgccaggc atcaatgcgc ggacgatcgg cacggtcgta
     3001 atacggctgg agtcgtctgc cggtcagcag ctccgtattc gggatcagga agttcagctc
     3061 cagccgccct ttgtcctggt gctcaaccca caacacgctg tactggtctt tatcgagtcc
     3121 gggcatcaga acccgctcga agctcgccat cagttcttca cgctggccgg gcactaagtc
     3181 ctgttcggcg aaggacagga caccagaggt gtactttttg gcatacgggg aggcatcgat
     3241 aagctcccgg atttcctccg gttttccctg tagaacggtg gcaccgttac gctgccggtc
     3301 tttgcccagt agataatcca ccggaccggc gccaccgcca ctgcctcgag gatggaactt
     3361 aacgatcatc ctcagcaccc ttctccaata cggcgtgccg tagccgctcc agtccggcat
     3421 caatggccat cagcgcagcg acaacctgca cacggtcatg tccgctgcct gcaccggcgt
     3481 taacctgccg ggcgatctga ttaaggttgt tacccatgcc agcaaactga cgaagcaaag
     3541 ccggtgaaat cgaaggaagt ttgccagtgc gggcgggttt ctcatccaga cacgtctgcc
     3601 gcatccaagt agcaagttgt tttccgtcac agcgttctag caaacgacgg tgctcatctt
     3661 cagtcaccca cattgtgagc attttgctgc gtttgtctgc caatctggcc tcctgataac
     3721 gccaaggcgg acgggaaatc ttttcccggt cggtgcggtg ttggacaagc cgcaggcgcg
     3781 tcagtcgctt ttagccggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga
     3841 gtgtatactg gcttcctatg ttagttcgag ttggtgattc aggaaagtgc gtcatgtgac
     3901 agggggaaat gcagcaaaaa tgcgtaggta gaaaatacaa gaaaggatga aatgacgcct
     3961 cctcgctcac tcggtcgcta cgctcttgcc gtgagactgc ggcgggcgtt acctgctcac
     4021 agaggagggg ggaaagagat gggtcggagg tcggagagaa caggaaaagg atacggcgat
     4081 gccgtttttc cataggctcc gcccccctga cgagcatcac aaaacctgac gctcaaatca
     4141 gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct
     4201 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     4261 gtttctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
     4321 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgacttga
     4381 gtccaacccg gaaagacacg acaaaacgcc actggcagca gccactggta actgatttag
     4441 aggagttagt cttgaagtta tgcgccggtt aaggctaaac tgaaaggaca agttttggtg
     4501 actgtgctcc tccaagccag ttacctcggt tcaaagagtt gctgctctga gaaccttcga
     4561 aaaaaccgcc ttgctgggcg gttttttcgt ttacagtgct aagagattac gattctccat
     4621 gaatgatctc aagaagatcc gtttaaaaaa gcgggtttta tggggggata aagttcgtct
     4681 agcccagtca ctttggatta cagctaggtt tcgtaagcca tgcaagaatg cctgtaacta
     4741 acaaaatagg ccctgcaacg tacgtaacat agactccaac atacgacact gtgaaacata
     4801 tgagtacgta tatagacagt gttgtccaaa aaatgactgt tgttttacag tgaatccact
     4861 ctttcatttt tcaataaacc ccacaccgtc tggagatctt attatcgttt tgccaggatt
     4921 agctttcgcc caaaccaaag catcttgaaa agaagagaaa tttttttttg aatttttagt
     4981 ttttcggcga tcttctcgaa aatattcgct gttattctca tcaattatcc gatcaaaatc
     5041 agcaccagct ccaaaggtac gctcaaaatc ccccattttc tgcccccatt aaatttaata
     5101 aaaaaacaga tttaaaatat ccaaaagaca ctcttattta ccataacatc ccttgttcgc
     5161 accgccgcag acagcctcag caccgttctg cgccatgaac cgcaaaagag cacctcttac
     5221 cacgtaaaaa cgccgataaa tgccccccgt aagttcttac ggtatgcata gttatgcacg
     5281 gaaatgaact gacactctgt tcaatggatt ttcatgctgt aagcagtatt ttagtcttct
     5341 atgccccctc gaaacacacc tgttcttgtg tagatctttt cgacccaaga tggtctttta
     5401 tccgagttca tgcagccgta caggaacaca cctgttttgg gtttatagct ccccccctcc
     5461 tgtggctggt tatctacaaa tgaataatca atcttttcac ctttgactga agcacgcccc
     5521 ccaacatgaa ctaaagacca gtgctcatgc atcacatggc tttcaatcaa tccaaatgaa
     5581 gaaaaacccc atgaatcaaa aaagaccttg agtccgtcag acgcaccaac aggagtaatg
     5641 attgcaaatt caacctgctc accagtacct tcaaggtaag aaggtttctc gacgttaatt
     5701 acagatgccc ccttcacctg ataaccttta caggtaagat ataagtccct cccggctgac
     5761 ggttttgtat atgtaacgtt gtcgttattc tccgcagcag cgactgtaaa tgacatagcc
     5821 aaaaccaccg agactgagca agcccagaaa catttcataa cgcatccctt ttcattgatt
     5881 aactacattc caagctcaaa acctttctgc cgctgcgatc gttccagctc cagcgctttc
     5941 tggtgctgcg ccgcgcgctc ctgctcccgg tgcgcctcga tgccattcag ccttgcgaca
     6001 atcggcgcag ctgatcgctc aaactctgca cctgcctgct caagcccgtg acgcgctcgc
     6061 tcagcgccgc gttctcccgt tgcataagcc cagacatcgt ccgccattcc gcgaaggcgt
     6121 tctcccactc gtccagcctt ttcgagtagt cctgctgtag ctgctctaat gcgctcagca
     6181 actgtttttc cagttccgtc atgtgctatt tccccgccag tatcatccag ctcgaatcct
     6241 ctccgaatct tcccgcttcc gggttcaccc tcacgcagcg gcgtctctcc tccccgcagg
     6301 tcgtgagctg gacgccgttg ttcttctctg aaagcatggc ctgcgtgctc ttctgttcct
     6361 ggatggtcgt gtagttgtcg agtatctgtt gcccctgcca ccacagaatg cccgcgctcg
     6421 aggcgatcag tagcgcggag accaggacta tggtcagcca cgtctggctg atcatacgca
     6481 gcatcccttt cgtgcgttgg ctcatggctg aggacagctt ccggtcgtgg tcgaggatgg
     6541 cggcgctgat tctcttctcg ctttcgctca gttccgctct gacagacttc tcgtgctcgc
     6601 tgaatgcggc tttcagcatc tcgccggtag tctgctgctg cgctttcgat ttctgctcta
     6661 agtccttcgc cagcgttaaa agactgttca tagatggctc ccttcagtcg gatgtttcgc
     6721 cccccgtcag ggtcggcaat gctgatgctg cttttcgtgg tgcgcaccac ctcaaaaccc
     6781 gcactttcca gcgcctcagt gacgtcctga cgggttttca gctccccgga tgaggcaagg
     6841 gaaagtaggc cgcgcgtaat cgcctgggcg gcttcctgct tcgcttccgg cagcgcggac
     6901 ggtgtaacca gcgcccgccg gttttccggc gcgttcgggt cgtgcagccc cagtctgccg
     6961 ttcactatgg tctgccaggc atcaatgcgc ggacggtcgg cgcggtcgta atacggctgg
     7021 agacgtctgc cggtcagcag ctccgtgttc gggatcagga agttcagctc cagccgccct
     7081 ttgtcccggt gctcaaccca cagtacgctg tactggtctt tatcgagtcc gggcatcaga
     7141 acccgctcaa agctcgccat cagcttttca cgctggccgg gcggcaaatc ctgttcggca
     7201 aaagacagca cgccggaggt gtatttcttg gcgtaaggcg aggcgtcaat aagctcccgc
     7261 acttcctccg gcttaccctg cagaacgctg gcgccgtcgc gctgccggtc tttccccagc
     7321 agataatcca ccggaccgcc gccaccgccg cggcctctgg gatgaaactt aacgatcatc
     7381 gtctgccccc ttttccagca cggcgtgccg cagccgctcg agtccggcat caatggccat
     7441 cagcacggcg acaacctgca cgcgatcgtg cccagtaccg ccaccggcgt taacctgccg
     7501 ggcaatctgg ttgaggttgt tacccatgcc ggcaagctga cgaagcagcg ccggcgagat
     7561 ggatggaagt ttgccagcac gcgagggctt ttcgtccagg catatctggc gcatccaggc
     7621 cgcgagttgt ttaccctcac agcgctccag aagtcggcgg tgctcgtctt ctgtcaccca
     7681 catagtgagc attttgttgc gcttttctgc caaaccatcc tccggataac accggttcgg
     7741 gcgggaaatt ttttcctgtc cggagcggtg ttgggcaagc ccgcagggcg cggcagtact
     7801 tttcagcggg tttcggggcg cagccctgaa ccagtcacgt agcgctagcg gagtgtatac
     7861 tggcttaaca tggtaaatcg gagtggggat tcaggaaagt gcttcatgtg gcagggagaa
     7921 aatgctgctg tggggcgtaa gtgcgaaatg cagatcccgg tgaaatgacg cctcctcgct
     7981 cactcggtcg ctgcgctcct gccgtgagac tgcggcgggc gttgccagct tacgaatggc
     8041 tggataaaga gactaagcgg aaatcaatca gaaagggcgg aaaaaggatg cggcgttgcc
     8101 gtttttccat aggctccgcc cccctgacaa gcatcacgaa atctgacgct caaatcagtg
     8161 gtggcgaaac ccgacaggac tataaagatc ccaggcgttt ccccctggta gctccctcgt
     8221 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     8281 atctcattcc acgcctgaca ctcagttccg cgaaggcagt tcgctcctgg atggactgta
     8341 tgcacgaacc ccccgttcag tccgactgct gcgccttatc cggtaactat catcttgagt
     8401 ccaacccgga aagacacgac ttatcgccac tggcagcagc cactggtaac tgatttagag
     8461 gagttagtct tgaagttatg cgcctgttaa ggctaaactg aaagaacaga tttggttact
     8521 gcgctcctcg taagccagtt accacggttc aaagagttgc tgctctgaga accttcgaaa
     8581 aaccgccttg cagggcggtt ttttcgttta cagagcaaga gattacgacg ccacagaaag
     8641 gatctcaaga agatccttca ccgtaatgga aatctatcca atccgagcac ataggataca
     8701 catcgttata acgctggatc tgcgtgaatt ccagcagcag caagagaaag actttctaca
     8761 aaccagctta cagcaggcta aatttaacca gaaaaaagcc gctgaattac tgggtctgac
     8821 ttatcatcag ctacgtgcct tgctgaagaa acaccagatc taatacgcat ttgcaggtgt
     8881 catactggcg ggtttgacgc ataacgtcat aaggtttacc atggcgtcat tactgtataa
     8941 aaacacaggc atagatatga ctctggcatt agttggcgaa aaaattgaca gaaatcgctt
     9001 caccggtgag aaagttgaaa atagtacatt ttttaactgc gatttttcag gtgccgacct
     9061 gagcggcact gaatttatcg gctgccagtt ctatgatcgc gaaagtcaga aagggtgcaa
     9121 ttttagtcgc gcaatgctga aagatgccat tttcaaaagc tgtgatttat caatggcaga
     9181 tttccgcaac gtcagtgcct tgggcattga aattcgccac tgccgcgcac aaggcgcaga
     9241 tttccgcggt gcaagcttta tgaatatgat caccacgcgc acctggtttt gcagcgcata
     9301 tatcactaat actaatctaa gctacgccaa tttttcgaaa gtcgtgttgg aaaagtgtga
     9361 gctgtgggaa aaccgctgga tggggactca ggtactgggt gcgacgttca gtggttcaga
     9421 tctctccggc ggcgagtttt cgactttcga ctggcgagca gcaaacttca cacattgcga
     9481 tctgaccaat tcggagttag gtgacttaga tattcggggt gttgatttac aaggcgttaa
     9541 gttagacagc taccaggcat cgttgctcat ggagcggctt ggcatcgctg tgattggtta
     9601 gttcagcgtt tacaccggga gatgcgtttg acgatttact tttaaccagt acgactcctg
     9661 gcaagcattg tgaaacgttt tctccagtgg acgaatatcc tgacctaccg catcaacgac
     9721 atgccatcta atgctgcatt ctggacagga atttgatgaa tatctaacca attcattttc
     9781 gcgagaacaa cgaataacca ctcccgaatc atgaagccat gtcttaacga aaatatggct
     9841 aactcatagc aaaaaaaaag ccccctaagg gctttttttt gtacctgacg gtcagaccga
     9901 ttaaacataa catcccttgc gtgcaccgcc tgagaggctc tgagggcgtt tctgacggga
     9961 aaccggtaaa acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt
    10021 ttacggcatg catgagtatg cgcgcaagtg catagcgcgg aaattcacac cattcagcca
    10081 ggggaagtcc tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta
    10141 ttgcgtggcc gtgaagcgtt gccggcgcag ccggaaaacc cgctgtcagc acgttcagga
    10201 agggggtgat gtacaaatgg cacagaggat gaaagtgacg tatgagctga aggaatgatg
    10261 taaaagtgat gtataaatga tgtaaaagtg atgcaaaagt gttgtaaaag tgatgtacag
    10321 gccgaaggag tgttgtaaaa gtgttgtaaa agtgatgtat gaactgaagg agcgatgtaa
    10381 ttctgtagtc aggaagaata acccgttcat gcaggcatga acgatacagt gaggaacgct
    10441 tttttttgtc acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt
    10501 caacctcaaa atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt
    10561 aaaagaaaaa aaagtaatgt ttcgttttca cgagtaatac ccaggtatta aaaacccact
    10621 gtaattacat gaaaataaag aaataaaagg taataccggc gtattaccac gtacgcgggc
    10681 aagcgaaaaa gctataacgc ttcccggccg acaggcggcc cagaatccac aggggcatgc
    10741 aggatctctt cagtaacctt cgccattaaa tcaaaaaggt catattcgcc gggaagctgc
    10801 gtatcagtcg aaccccgttg ttcatcatat tcagatggca tggggtgata tccagtagca
    10861 gagaggttgt tagcgggttt cggggcgcag ccctgaacca gtcacgtagc gctagcggag
    10921 tgtatactgg ctgactatgt tggcacggat gtggaaatca gtgaagtgct tcatgtagca
    10981 ggagaaaaaa ggcggcacca gcgcgtcagc aggatatgta gtacaggata tattccgctt
    11041 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggtg ccggcttaca
    11101 ggcggggcgg aaatttcctg ggagatacca ggaaggagct taacagggaa gagataaggc
    11161 cgcggcgaag ccgtttttcc ataggctccg cccccctgac aaacatcatg aaatctgacg
    11221 ctcaaatcag tggtggcgaa acccgatgcg gcttaaagat accaggcgtt tccccctggt
    11281 ggctccctcg tgctcccccg cattcctgcc tttcggttta ccggtgtcat tccgctgtta
    11341 tggccgcgtt tatctcattc cacgcctgac actcagttc
//
