LOCUS       NZ_CP069465             1763 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1249 plasmid unnamed6.
ACCESSION   NZ_CP069465
VERSION     NZ_CP069465.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357391
            Assembly: GCF_016889125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 864.31x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 04:58:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,157
            CDSs (total)                      :: 5,036
            Genes (coding)                    :: 4,746
            CDSs (with protein)               :: 4,746
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 290
            CDSs (without protein)            :: 290
            Pseudo Genes (ambiguous residues) :: 0 of 290
            Pseudo Genes (frameshifted)       :: 76 of 290
            Pseudo Genes (incomplete)         :: 208 of 290
            Pseudo Genes (internal stop)      :: 41 of 290
            Pseudo Genes (multiple problems)  :: 34 of 290
            Pseudo Genes (short protein)      :: 4 of 290
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069465.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1249"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1249"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(369..665)
                     /locus_tag="I6J79_RS25290"
                     /old_locus_tag="I6J79_25290"
     CDS             complement(369..665)
                     /locus_tag="I6J79_RS25290"
                     /old_locus_tag="I6J79_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_000609270.1"
                     /translation="MINLVDPVHPGVFIREMFMEPFEISAADLSEKLHVSPSTLSRVLN
                     GKADLSVEMALRLSRVLGRSAESWINMQTQYSLNASRKSPNVIKELDELVPIF"
                     /name="HigA family addiction module antitoxin"
     gene            1010..1291
                     /locus_tag="I6J79_RS25295"
                     /old_locus_tag="I6J79_25295"
     CDS             1010..1291
                     /locus_tag="I6J79_RS25295"
                     /old_locus_tag="I6J79_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000586174.1"
                     /translation="MIINIKHKGLRKFAETGNNAGIPQQFTQRLKLILTALSTATNLQD
                     MMDLKALRCHPLKGDRQGEYSLEVNGNWRVVFKLEEPNVFDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2..95)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.947"
                     /coverage="87.0"
                     /evalue="1.96e-40"
ORIGIN
        1 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
       61 cttatccggt aactatcatc ttgagtccaa cccggaaaga catgcaaaac accattggca
      121 gcagccactg gtaactgatt tagaggagtt agtcttgaag ttatgcgcca gttaaggcta
      181 aactgaaaga acaagttttg gtgactgcgc tcctccaagc cagttaccct ggttcagaaa
      241 cttgccggtt actgaacctt cgaaaaaacc accttgccgg gtggtttttt cgtttacgag
      301 aaacgatacg atccagttgc ctgagagtca agacagaaaa aggccgcata tagcggcctt
      361 ttcttttctc aaaatatagg aaccaattca tctaattcct taatgacatt aggtgacttc
      421 ctgctcgcgt taagactgta ttgagtctgc atgtttatcc acgactcggc actgcgacca
      481 agaactcgag aaagccttaa tgccatctca acacttaaat cagcttttcc attcagcact
      541 ctacttagag tggaaggtga tacatgcagt ttttcagaca ggtccgcggc tgatatttca
      601 aacggttcca taaacatctc acggataaaa acccctggat gtactggatc gacaagatta
      661 atcatacctt cctctgaagc ctatattagt tttaaaaaga taggcctatt gcgttttcag
      721 attgcgcatt gcgcataacg caactcattg ttgactaaaa aaaagaatga cattatgatg
      781 cacattggtg cgtctaatca ttccttatga tgtgggtact tgatgtgaaa ttcaaggccc
      841 cggcattgta cagcgcatca aaccaatgtc aacagattaa aatgcgactt ggttaagtaa
      901 aacctacgtc atttattcaa atagagcaag gggtttgcaa tggaaatacg gatgaaccgg
      961 aaaaataaac tgcgatgttt ctatctaaac aaatatggga tggtgattaa tgatcataaa
     1021 tatcaagcat aaaggtttgc gtaagtttgc cgaaacaggt aacaacgcag gaattccgca
     1081 acagtttact caacgtctga agctgatcct aacagcctta agcacagcga cgaacttgca
     1141 ggatatgatg gatttaaagg cattgcgctg ccatccattg aagggagacc gtcaaggaga
     1201 gtattcgtta gaggtcaatg gaaactggcg cgtagtgttt aagctcgaag aacctaacgt
     1261 ctttgacgtg gatctcgagg attatcacta agaaggtcct taaagcatca aagtgacagt
     1321 atcgatgctt tagggtctca taaaaaagca aaacctcggg gtaagttata tgaagtattt
     1381 tctgtaccag atagtcccta tagcagccat cattagcgca gcaattctag cgctaaaagg
     1441 tatcaatggc tgggggtggt tcttaactgt tgggttcctg cttgcagggg cagatacagt
     1501 aaaggctcag ttatctaagt agtaataaac actatcaata gtggttggat aattgccaaa
     1561 aaagcctcta tctgttctga aacagacaac aaggaaaaat cggtgtaagc cacctccata
     1621 ggaggtggta ttgatatcaa catagataaa accatcccgt aaatcacagc atttaccata
     1681 acgtcgcatt acgcgcaccg ctaaaacgcg ctgaacgcga ttctgccgcg aacaccgcaa
     1741 aaagagatgt tttaccaagc gaa
//
