LOCUS       NZ_CP069449             2159 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1255 plasmid unnamed2.
ACCESSION   NZ_CP069449
VERSION     NZ_CP069449.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357397
            Assembly: GCF_016889045.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.14.1
            Genome Coverage                   :: 758.31x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 04:32:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,893
            CDSs (total)                      :: 4,777
            Genes (coding)                    :: 4,572
            CDSs (with protein)               :: 4,572
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 82 of 205
            Pseudo Genes (incomplete)         :: 129 of 205
            Pseudo Genes (internal stop)      :: 44 of 205
            Pseudo Genes (multiple problems)  :: 47 of 205
            Pseudo Genes (short protein)      :: 4 of 205
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069449.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1255"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1255"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..282
                     /gene="mobA"
                     /locus_tag="I6J85_RS24185"
                     /old_locus_tag="I6J85_24185"
     CDS             <1..282
                     /gene="mobA"
                     /locus_tag="I6J85_RS24185"
                     /old_locus_tag="I6J85_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_059319437.1"
                     /translation="SRPVRLTAEEDQEIRKRAAECGKTVSGFLRAAALGKKVNSLTDDR
                     VLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRLMAD"
                     /name="mobA"
     gene            complement(671..1099)
                     /locus_tag="I6J85_RS24190"
                     /old_locus_tag="I6J85_24190"
     CDS             complement(671..1099)
                     /locus_tag="I6J85_RS24190"
                     /old_locus_tag="I6J85_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            1257..1427
                     /locus_tag="I6J85_RS24195"
                     /old_locus_tag="I6J85_24195"
     CDS             1257..1427
                     /locus_tag="I6J85_RS24195"
                     /old_locus_tag="I6J85_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            1391..1819
                     /locus_tag="I6J85_RS24200"
                     /old_locus_tag="I6J85_24200"
     CDS             1391..1819
                     /locus_tag="I6J85_RS24200"
                     /old_locus_tag="I6J85_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(2035..>2159)
                     /locus_tag="I6J85_RS24555"
     CDS             complement(2035..>2159)
                     /locus_tag="I6J85_RS24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_238067946.1"
                     /translation="PPVQPDRCALSGNYRLESNPVRHDLTPLATATGKQDVREM"
                     /name="replication initiation protein"
     Typing          2..279
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.925"
                     /coverage="87.0"
                     /evalue="1.81e-53"
ORIGIN
        1 agtcgccctg ttcgtctgac ggcagaagaa gaccaggaaa tcagaaaaag ggctgctgaa
       61 tgcggcaaga ccgtttccgg ttttttacgg gcggcagctc tcggtaagaa agttaactca
      121 ctgactgatg atcgggtact gaaagaagtt atgagactgg gggcgttaca gaaaaaactc
      181 tttatcgacg gcaagcgtgt cggggacagg gagtatgcgg aggtgctgat cgctattacg
      241 gagtatcacc gtgccctgtt atccaggctt atggcagatt agcttcccgg agagaaaact
      301 gtcgaaaact gacggtatga acaccgtaag ctcccaaagt gatcaccatt cgctttcatg
      361 catagctatg cagcgagctg aaaacgatcc tgacgcatcc ttcctgtttt tccggggtaa
      421 aacatctctt tttgcggtgt ctgcgtcaga atcgcgttca gcgcgtttca gtggtgcgta
      481 caattaaggg attatggtaa atatatgagc tatgcgataa ctttaactgt gaagcgatga
      541 acccattaca ggcaaagcca attactcctg acagtggttt agccagaagc agggctacca
      601 agaccaatgc aataagtaat atatcgtttt gctatcgtgc atcgctcgcg ctcagttcca
      661 ttgtgctttt ttaagctgtc gtttttctta cggtatatac cggtttttta tggcgtggtt
      721 tcttaacttg ttcagctact gatggaccca ttgtatctag gtagtcaact agctttgtta
      781 gatcataaat attgcgacca ccatatcggc gatcactctt cgatgttggt ttcttgtcca
      841 agagattagc tttttcaaga tcattgatag ctctctgaac agtccgtaca gaaaccccca
      901 tacgtatggc tagactttcc attgacggat gcggccactc ttgcaaactc caccagtgaa
      961 cgatcaggtt aagtagtgtg ttaaaggcca ctgaagttag ctttttctcg ttttgtataa
     1021 aaaacaatac ggtaggcact gctgtccagc caagagacaa accgccagct ttccatttat
     1081 tcttaacgga gtaagtcatt gattttccta agccccaaaa tatttaaagt atatattata
     1141 tggtatattc atatgaatag ggtgacactg gcgccattat tgtgcaacca aaaaagacta
     1201 ctctgaaaac gaggaaaaga ttttttcctg cctgaattag atacggagtt agcgatatga
     1261 aaaccgaaca acgtcatgat cttgttaaag atattgaggt ttttggcgta tccttgtctc
     1321 tgttgatttc cagagcgaat gagaagtctg ttacaatgcc atctggtcta agtcgggagc
     1381 agagaagagc atgggcagcg gagcaggcgc gcaaaatcca caattgaata ttgtctcatt
     1441 ctctgagacc ttcaaccttt attacacatc cagatattct gcaaaaacac tcgataaaat
     1501 cgatgatttc attgagcatt ttgaaaaata caatctcttt ggcgatcctt taaaaggata
     1561 tccagcttgg actggcaaag tatcgccatc gtggaaagtg cctgatcatt acgaaaacaa
     1621 agaagctatt gagaagtatg ctagagctaa caaattatgg catgctcatt taggcgatcc
     1681 ggtttttaaa gatacgtttc atgggaaata caaggtttct gactgggtta ttcatttcca
     1741 gcggctgaca ccgaaccata taaagctttt agagcttgga taccatgacc caatgaagct
     1801 acctcaaaac tttgaatgat cgagcggcag gctaaatgaa atcttgagat tcattcagtc
     1861 tcgtcgtaat ctcactattg taaaaacgaa aaaaccaccc tggcaggtgg tttttcgaag
     1921 gttagttaat cctggcagat tctctaaccg tggtaacagt cttgtgcgag acatgtcacc
     1981 aaatactgtc ctttcagtgt agcctcagtt aggccgccac ttcaagaact ctcgttacat
     2041 ctctcgcaca tcctgcttac cagtggccgt tgccagtggc gttaagtcgt gtcttaccgg
     2101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggtt
//
