LOCUS       NZ_CP069417             1680 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia megaterium strain CDC 2008724129 plasmid pPmC-129-20,
            complete sequence.
ACCESSION   NZ_CP069417
VERSION     NZ_CP069417.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16083678
            Assembly: GCF_017086545.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Shwed,P.S., Crosthwait,J., Weedmark,K., Hoover,E. and Dussault,F.
  TITLE     Complete Genome Sequences of Priestia megaterium Type and Clinical
            Strains Feature Complex Plasmid Arrays
  JOURNAL   Microbiol Resour Announc 10 (27), e0040321 (2021)
   PUBMED   34236233
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Shwed,P., Crosthwait,J., Weedmark,K. and Dussault,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Biotechnology Lab, PS-Biotech Lab, 251 Sir
            Frederick Banting, Ottawa K1A0K9, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017086545.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 06:19:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,820
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 199
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 148
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 24 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 25 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069417.1.
            Bacteria and source DNA available from CDC; Dr. C. Beesley.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Priestia megaterium"
                     /mol_type="genomic DNA"
                     /strain="CDC 2008724129"
                     /host="Homo sapiens"
                     /db_xref="taxon:1404"
                     /plasmid="pPmC-129-20"
                     /geo_loc_name="USA: Rhode Island"
                     /collection_date="2005/2006"
     gene            74..1633
                     /locus_tag="ICR95_RS28925"
                     /old_locus_tag="ICR95_28925"
     CDS             74..1633
                     /locus_tag="ICR95_RS28925"
                     /old_locus_tag="ICR95_28925"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="WP_205663991.1"
                     /translation="MNLQSLYKDWILLFSKINDSDIQLYLQANQSSNHCPSCQTISSRI
                     HSRYWRTVKDLSILSLSVHLHVRSRKFFCDNTSCDQRIFTERQEKWWLPYARRTNRLSS
                     FLKQLGFTLSAEAAHKVSVSYGTSISPDSFLYLIRKEELPSIGSPKIVGIDDWAFRKRH
                     RYGSVICNLQTRKPIALLPSRTVEEVSKWLKAHPSIEIVTRDGSIEFAKGIKDGCPGAI
                     QITDRWHLFHNLSQKVEQYVKRIFPHRISLSCRVKRREEKVNSLLSSLPSLTEGEKRKW
                     AFIQRVQERYKEGCKKADVIREFSIDRKTLNKYLQLTCPPKVTRESFHSADPYRSTIVS
                     LLQKRATIPYIFSVIKQQGYDRSFSTLSDYITKLKRRRKQNVSVTKVFVSRLKLQAYLW
                     SRLTPTKKDQFIFNRLFKLHKSFHQLKCIIQGFQQLIYHQKNEQSLMNWLSNAQKSNIN
                     EIKQFVAYIQTDIEAVKHALSYSWSNGIVEGNVNRLKVIKRQMYGRAKLDLLSKKVLYQ
                     FQ"
                     /name="ISL3 family transposase"
ORIGIN
        1 ggttctcccg taattttggt gaaagctcct gcatgatatt atagtagtaa acagtcatgt
       61 gggagggatt tagatgaatt tacagtcact gtataaggat tggattctat tattctctaa
      121 gataaacgat tctgatattc aactttatct tcaagctaat caatcttcta accattgtcc
      181 atcgtgtcaa acaatttctt ctcgtattca cagtcgttac tggcgaaccg ttaaagatct
      241 gtctatttta tctttgtctg ttcatttaca cgtccgttct agaaagtttt tttgtgataa
      301 cactagctgt gatcagcgaa tttttacaga aaggcaagaa aaatggtggc ttccatatgc
      361 tagacgcacc aataggctta gctcttttct taaacaactt ggttttacat taagcgcgga
      421 agcagctcac aaagtttctg tttcctatgg aacatctata agccctgata gctttcttta
      481 ccttatccga aaggaagaac tgccttccat tggttctcct aagattgtag gaattgatga
      541 ctgggctttt cgaaaaagac atcgttatgg cagcgtcata tgtaatttac aaactcgaaa
      601 acctatcgca ttacttccaa gtcggactgt ggaggaagta tcaaaatggc taaaggccca
      661 tccttccatt gaaatcgtca cacgagatgg gtcgatagaa tttgcgaaag gaattaaaga
      721 cggatgtcca ggtgctattc aaattacgga ccgttggcat cttttccata acttaagcca
      781 aaaagtagag cagtatgtga agagaatatt ccctcatcga attagtctat cttgccgggt
      841 gaaaagaaga gaagaaaagg ttaattcctt attgtccagt ctacctagtc tcaccgaagg
      901 tgagaaaagg aaatgggcgt ttattcaacg tgttcaagaa cgatacaagg agggatgtaa
      961 aaaggctgat gtcataagag aattttcaat tgatcgaaaa acattgaata aatacctaca
     1021 gttaacatgc ccgcctaagg tcacaagaga aagctttcat agtgcggatc cttatcgttc
     1081 gactatagtg agccttcttc aaaagcgtgc aaccattcct tatatctttt ctgtcatcaa
     1141 acaacaaggg tatgatcggt cgttctctac attaagtgat tatattacaa aattgaaacg
     1201 aagaaggaaa caaaacgttt ctgtcaccaa ggtttttgtg tctaggttaa aattgcaggc
     1261 ttacttatgg tctcgtctta ctcctacaaa gaaagatcaa tttatattta atcgtttatt
     1321 taagttacat aaaagctttc atcaactcaa atgcattatt caaggattcc aacagctcat
     1381 ctatcatcaa aaaaacgaac aatcactcat gaactggctt tcgaatgccc aaaaaagcaa
     1441 tataaatgaa ataaaacaat ttgtcgcata tattcaaaca gatatagagg ctgttaaaca
     1501 tgcattatcc tattcttgga gcaatggtat tgtggaagga aacgttaatc gtctgaaagt
     1561 tattaaacgt caaatgtatg gacgtgcaaa attagattta ttgagtaaaa aagttctgta
     1621 tcaattccaa taatgttttc tgaaaaaaac gttagacaac atcaatactt ctactcgatg
//
