LOCUS       NZ_CP069416             1702 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia megaterium strain CDC 2008724129 plasmid pPmC-129-19,
            complete sequence.
ACCESSION   NZ_CP069416
VERSION     NZ_CP069416.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16083678
            Assembly: GCF_017086545.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Shwed,P.S., Crosthwait,J., Weedmark,K., Hoover,E. and Dussault,F.
  TITLE     Complete Genome Sequences of Priestia megaterium Type and Clinical
            Strains Feature Complex Plasmid Arrays
  JOURNAL   Microbiol Resour Announc 10 (27), e0040321 (2021)
   PUBMED   34236233
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Shwed,P., Crosthwait,J., Weedmark,K. and Dussault,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Biotechnology Lab, PS-Biotech Lab, 251 Sir
            Frederick Banting, Ottawa K1A0K9, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017086545.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 06:19:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,820
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 199
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 148
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 24 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 25 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069416.1.
            Bacteria and source DNA available from CDC; Dr. C. Beesley.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Priestia megaterium"
                     /mol_type="genomic DNA"
                     /strain="CDC 2008724129"
                     /host="Homo sapiens"
                     /db_xref="taxon:1404"
                     /plasmid="pPmC-129-19"
                     /geo_loc_name="USA: Rhode Island"
                     /collection_date="2005/2006"
     gene            complement(join(1309..1702,1..698))
                     /locus_tag="ICR95_RS28920"
                     /old_locus_tag="ICR95_28920"
     CDS             complement(join(1309..1702,1..698))
                     /locus_tag="ICR95_RS28920"
                     /old_locus_tag="ICR95_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_205664588.1"
                     /translation="MNSLDFSTGEILVDKTATGKERPWENKKIRAEITAKHFEEAGLFS
                     KAKRMDQCADMLVFRHNGEKLKLHQTWFCKVRLCPMCNWRRSIKIATQNKKIVETVNEQ
                     EKVKWLFLTLTVRNCSAEKLEDTVQAMFKGFNRLTKYKDVKTAVRGFFRALEVTRDRDK
                     KITEKRFKTNPGYYKRQGLDVGSENPNYGTYHPHLHVLLCVKPSYFTRDYIKQSDWTDM
                     WQRAMKVEYKPIVHIQKVKPQKEIDSNELEKYEQDFKNAIKEQNAILEVSKYPVKDTDI
                     IHGKEVTPENVETVLDLDNALAYKRLIGYGGLLKEVHQELNLDDGEDGDLVHVSEEDEL
                     ANGIFEVTARWHIGIKKYVIEKE"
                     /name="protein rep"
ORIGIN
        1 ggtttgtact ctactttcat ggcacgctgc cacatatcag tccaatcaga ttgctttatg
       61 taatctctcg tgaagtaact cggtttaaca cataacaaca catgaagatg agggtgatac
      121 gttccgtaat taggattttc agacccaacg tctaaacctt gtcgcttata atagccagga
      181 ttcgttttaa aacgtttctc tgtaattttc ttatctcgat ctcttgtaac ctctaaagcc
      241 ctaaaaaagc cccttacggc cgttttaaca tccttgtact tagttaacct attaaaaccc
      301 ttaaacatgg cttgtacggt atcttcgagc ttctcagcac tacaattccg aaccgtaagc
      361 gttaaaaata accacttcac cttttcttgt tcgttaaccg tttctacgat ttttttattt
      421 tgagttgcga tttttatact tcttctccag ttacacattg gacataacct cactttacaa
      481 aaccatgttt gatggagttt taatttttct ccgttatgcc taaacaccag catatcagca
      541 cattgatcca tgcgtttagc tttggaaaaa agtccagctt cctcaaaatg tttcgcagta
      601 atttctgcac gaattttttt attctcccaa ggtctttctt tccctgtagc agttttatcc
      661 accaaaattt caccagttga aaaatccaat gagttcatgt tattattctt tttagaagac
      721 acgaaaaaaa cctacctttc aaatgatttt gactcgacat cttaatcata gcaggtaggt
      781 ttttcgctgt ctacacataa aaacaggctt cagcccagtt ataccaaggc ttgaggcttt
      841 attttataaa tctcattctt tctaatagta tcaagataag aaaaaacaag ttcaaaacca
      901 taggcttaag cctatttaaa gcagctttaa gctgcttatt tttttgcctt taaatacttt
      961 gccaaatgat tttttatagt tatcaaatta gacaactacg aaaacaattt aaacaagcag
     1021 ggcgaaatta tacataactc cgttttgtca ttgcgaaacg attcaaatcg tttgattcgc
     1081 ttaagcgaaa ttgttctgcc tggttaaaaa atcgccagta aagcaaacgc ccatttacac
     1141 accacaaaaa acgtggtgat aaacgggcaa acccttcgct ttcagcgatt accttgcagg
     1201 acatgtttta tgtttttatt gcgaagcaat cgcacacatt ggaacaatgt ataagtgcgc
     1261 cctttatcac tttcgttcct cagggagaat aaaagagtag ccacctggct actctttttc
     1321 aatcacgtat ttttttatgc ctatatgcca ccttgccgta acttcaaaaa tcccattagc
     1381 cagttcatct tcttcagaaa catgaacaag atctccgtct tctccatcat ccaaatttaa
     1441 ctcttggtga acttctttta gcaaaccacc ataaccaata agtcgtttat acgccagtgc
     1501 attatctaaa tctaaaacgg tctctacgtt ttctggagta acttctttcc cgtgaataat
     1561 atccgtatct ttcactggat acttcgagac ttctagaata gcgttttgct ccttgatagc
     1621 atttttaaaa tcttgttcat atttttctaa ttcgttagaa tcaatttctt tttggggttt
     1681 aactttctga atatggacta ta
//
