LOCUS       NZ_CP069415             3251 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia megaterium strain CDC 2008724129 plasmid pPmC-129-18,
            complete sequence.
ACCESSION   NZ_CP069415
VERSION     NZ_CP069415.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16083678
            Assembly: GCF_017086545.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Shwed,P.S., Crosthwait,J., Weedmark,K., Hoover,E. and Dussault,F.
  TITLE     Complete Genome Sequences of Priestia megaterium Type and Clinical
            Strains Feature Complex Plasmid Arrays
  JOURNAL   Microbiol Resour Announc 10 (27), e0040321 (2021)
   PUBMED   34236233
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Shwed,P., Crosthwait,J., Weedmark,K. and Dussault,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Biotechnology Lab, PS-Biotech Lab, 251 Sir
            Frederick Banting, Ottawa K1A0K9, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017086545.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 06:19:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,820
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 199
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 148
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 24 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 25 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069415.1.
            Bacteria and source DNA available from CDC; Dr. C. Beesley.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Priestia megaterium"
                     /mol_type="genomic DNA"
                     /strain="CDC 2008724129"
                     /host="Homo sapiens"
                     /db_xref="taxon:1404"
                     /plasmid="pPmC-129-18"
                     /geo_loc_name="USA: Rhode Island"
                     /collection_date="2005/2006"
     gene            join(2257..3251,1..97)
                     /locus_tag="ICR95_RS28910"
                     /old_locus_tag="ICR95_28910"
     CDS             join(2257..3251,1..97)
                     /locus_tag="ICR95_RS28910"
                     /old_locus_tag="ICR95_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_116288527.1"
                     /translation="MDSLDFSTGEILVDKTSTGKERPWEDKKIRAEITAKHFEEAGLFS
                     KAKRMDQCADMLVFRHNGEKLKLHQTWFCKVRLCPMCNWRRSIKIATQNKKIVETVNER
                     EKVKWLFLTLTVQNCSAEKLEDVVQAMSKAFNGLTRRKAFKTAVKGFFRALEITRDRDK
                     EITEKRFKTNPGYYKRQGLGAGSENPNYGTYHPHFHVLLCVKPSYFTRDYIKQSDWTDM
                     WQRAMKVDYKPIVHIQKVKPKKEIDSSELEKYEQDFKNAIKEQNAILEVSKYPVKDTDI
                     IHGKEVTPENVETVLDLDNALAYKRLIGYGGLLKEVHQELNLDDGEDGDLVRVSEEDEM
                     ANGIFEVTARWHIGIKKYVIEKE"
                     /name="protein rep"
     gene            915..1379
                     /locus_tag="ICR95_RS28915"
                     /old_locus_tag="ICR95_28915"
     CDS             915..1379
                     /locus_tag="ICR95_RS28915"
                     /old_locus_tag="ICR95_28915"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_088568401.1"
                     /translation="MAKITMSNTRTITDEDGSIRYQEEDLTVNWGKEPNYIKLYLDTVL
                     YLKDLPKGLNTILYALLKRMSYGNQLVLNAALKRQIAEEIDLSVSSINNAITKFVKGEL
                     LERIDTGLYRVNPHLFGKGEWKDIAKIRLEVAFDSEGKTIMSEIERKEID"
                     /name="replication/maintenance protein RepL"
ORIGIN
        1 tcgtgtttct gaagaagatg aaatggcaaa tggaattttt gaagttacgg caaggtggca
       61 tattggtata aaaaaatacg tgattgaaaa agagtagcca tggggctgct cttttgtttt
      121 ccctgaggaa cgaaagtgac aaagggcgca cttatacatt ggatcaatgt gtgcggttct
      181 ttcagaacaa aaaaacaaaa acagagccta aacacttaaa agcaaaatga gcaacttaag
      241 ttgcttctgt gttggcttaa gccaacctta gttcttaaaa ttcttgctgc tacgtttcgt
      301 atccagtcat ctcaaaactc aaaatactta tataagccct caaatttcgt tttaaaggtt
      361 ttttgccttt taaaatatat ttcctatctc tcccgtaggg taaattgctt aaaaagcaat
      421 ctggcccatt tgaccatgat attttcatgg tgtgtaaatg ggcattgtgc ttctcaacga
      481 ttttttatta attgattatc aacttaaaaa tcaaattgag tttaaaaaat ataaatctat
      541 ttttagtttt taagtttttt tgtaaaaaac taaaagcatt tttttgtaaa aaaaagtgct
      601 ttttcgggtt aaaaacaccc tcaaaaacca gaccctcaaa aaaaccccca aataataaga
      661 gaagggaaaa agggggtaaa aatagccttc agactatggg ggacaaggct tagaggtgtt
      721 ttggaccatt cctccactca tgtaacaacg ttcctccact ggaggaatgt cattcctcca
      781 gtggaggaat acgtattcct atgatttata ggaatagttg atttatcagg gtttacaaac
      841 attcctataa aacgttataa tagtattaca aatatcttag gaatactttt cgaagtaaag
      901 gggaggatat aaaaatggca aaaatcacta tgagtaacac aagaactata actgatgaag
      961 atggatctat aagataccag gaagaggatc tcacagttaa ctggggcaaa gaaccaaatt
     1021 acataaaact ctacttagat actgttttgt atttaaaaga tttacctaaa ggcttaaata
     1081 caattttata tgcactttta aaaagaatgt cctacgggaa tcagctagtg cttaatgcag
     1141 ccttaaaaag gcaaatagcc gaagaaatcg acttgtctgt atctagtatt aacaacgcca
     1201 taaccaaatt tgttaaaggc gaacttctcg aaagaattga tacaggttta tatagagtca
     1261 atcctcatct tttcggtaaa ggtgagtgga aagatatagc caagataaga cttgaagtag
     1321 catttgatag tgaaggtaaa accataatgt cagaaatcga aagaaaagaa attgattaaa
     1381 ctaactaaaa agcccctaca ctcttgctgt agaggctttt taaagttatc tcaaagcata
     1441 aagagcctag tcaaaaatcc cttggataaa aaagacaagg gcgcacttat acattgttcc
     1501 aatgtgtgcg attgcttcgc aataaaaaca taaaacatgt cctgcaaggt aatcgctgaa
     1561 agcgaagggt ttgcccgttt atcaccacgt ttttttgtgg tgtgtaaatg ggcgtttgct
     1621 ttactggcga ttttttaacc aggcagaaca atttcgctta agcgaatcaa acgatttgaa
     1681 tcgtttcgca atgacaaaac ggagttatgt ataattgcgc cctgctaaaa accgcaggga
     1741 ataaaaatcc aaatactttt aagcaacagg acatctttta aaaattacgg gtttaaatat
     1801 gggtcgagtt ctctgttgct gtttgacaag tgcaaaggga aaagaatttt agcgaagcga
     1861 tcggaacgaa aaattgtttt gcgtttggcg gagcgaacac ttgccaaaga cttatggcca
     1921 ctgattgacc accagatacc ctcttgccct tcccaaaaaa gcgcattttt tgggatgcct
     1981 aacgcagtgg gtaagcagag tgcaacggaa cggttttgac tttgtttttt gcttttgatt
     2041 ttaggggttt ttaggggttt gtttttttgt tttttcttat cttgatacta ttagaaagaa
     2101 tgagtattaa taaaatggcc ctgaaacctt ggtatgactg ggtttcatct gttttttatg
     2161 tgtagacagc aaaaaaccta cctgttatga ttaaattgtc gagataaaat cataagaaag
     2221 gtaggttttt ttgtgttttc taaaaagaat aatagcatgg attcattgga tttttcaact
     2281 ggtgaaattt tggtggataa aacttccacg gggaaagaaa gaccttggga agataaaaaa
     2341 atccgtgcag aaattactgc gaaacatttt gaggaagccg gacttttttc gaaagctaaa
     2401 cgcatggatc aatgtgctga catgttggtg tttagacata acggagaaaa attaaaactc
     2461 catcaaacat ggttttgtaa agtgaggtta tgtccaatgt gtaactggag aagaagcata
     2521 aaaattgcaa ctcaaaataa aaaaatcgta gaaacggtta acgaacgcga aaaggttaaa
     2581 tggctgttct taacccttac tgttcaaaat tgcagtgctg agaagctcga ggacgtcgta
     2641 caggctatgt ctaaggcttt taatggctta actaggcgga aggcgtttaa aacggctgta
     2701 aagggctttt ttagggcgtt agagattacg agagatcgag ataaggaaat cacagagaaa
     2761 cgctttaaaa cgaatcctgg ctattataag cgacagggtt tgggtgctgg gtctgaaaat
     2821 cctaattacg gaacgtatca tcctcatttt catgtgttgt tatgtgttaa accaagttac
     2881 ttcacgagag attacataaa gcaatctgat tggacagata tgtggcagcg tgccatgaaa
     2941 gtagattaca aaccgatagt tcacattcaa aaagttaaac cgaaaaaaga aatcgattct
     3001 agcgaattag aaaaatatga acaagatttt aaaaatgcta tcaaggagca aaatgctatt
     3061 ttagaagtgt caaagtatcc agtgaaagat acggatatta ttcacgggaa agaagttact
     3121 ccagaaaacg tagagaccgt tttggattta gataatgcac tggcgtataa acgacttatt
     3181 ggttatggtg gtttgctaaa agaagttcac caagaattaa atttggatga cggagaagac
     3241 ggagatcttg t
//
