LOCUS       NZ_CP069414             4359 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia megaterium strain CDC 2008724129 plasmid pPmC-129-17,
            complete sequence.
ACCESSION   NZ_CP069414
VERSION     NZ_CP069414.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16083678
            Assembly: GCF_017086545.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Shwed,P.S., Crosthwait,J., Weedmark,K., Hoover,E. and Dussault,F.
  TITLE     Complete Genome Sequences of Priestia megaterium Type and Clinical
            Strains Feature Complex Plasmid Arrays
  JOURNAL   Microbiol Resour Announc 10 (27), e0040321 (2021)
   PUBMED   34236233
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Shwed,P., Crosthwait,J., Weedmark,K. and Dussault,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Biotechnology Lab, PS-Biotech Lab, 251 Sir
            Frederick Banting, Ottawa K1A0K9, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017086545.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 06:19:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,820
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 199
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 148
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 24 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 25 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069414.1.
            Bacteria and source DNA available from CDC; Dr. C. Beesley.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Priestia megaterium"
                     /mol_type="genomic DNA"
                     /strain="CDC 2008724129"
                     /host="Homo sapiens"
                     /db_xref="taxon:1404"
                     /plasmid="pPmC-129-17"
                     /geo_loc_name="USA: Rhode Island"
                     /collection_date="2005/2006"
     gene            complement(join(4277..4359,1..802))
                     /locus_tag="ICR95_RS28885"
                     /old_locus_tag="ICR95_28885"
     CDS             complement(join(4277..4359,1..802))
                     /locus_tag="ICR95_RS28885"
                     /old_locus_tag="ICR95_28885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_205664585.1"
                     /translation="MATIKHSATKQVNRLINYCEKRAGVTSSHNLIADDRKSVQKQIKL
                     TREMFNKTGGVEGHHFIQSFKPGEVSPEKANELGLELVKRMHPKREAVVYTHTDKGHIH
                     NHIILNSPSIEDGKKLSVTRKHYFEWQRQNDAICKENGLSIVTEKKKQINKTLAEYHIM
                     VKGKLPFKTHLRAAIDLTKVKCASFKELQTFLKDPKLDIDSKIQKGHYYFKTKEMTRFI
                     SGKQLGNEYTKEVLDQEFIKRQKQNEREFTPRFVTVKDESFTPRFATIEKSKGFTPRFA
                     DKRSKSQDLDREL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(766..1149)
                     /gene="mobC"
                     /locus_tag="ICR95_RS28890"
                     /old_locus_tag="ICR95_28890"
     CDS             complement(766..1149)
                     /gene="mobC"
                     /locus_tag="ICR95_RS28890"
                     /old_locus_tag="ICR95_28890"
                     /inference="COORDINATES: protein motif:HMM:NF039635.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_205664586.1"
                     /translation="MGRTHAIPAIRLNDVEEDKLYRLMQQHNMKKTELIRKLIMNARLK
                     APVIDKSVGLQMLTDIRKVGTDLNKMGNNLNQIAKHLNEKGLKEEHAERLNKTLHGMIK
                     EQQDLKEEVLKQWQQLSTLLQSK"
                     /name="mobC"
     gene            complement(1660..2538)
                     /locus_tag="ICR95_RS28895"
                     /old_locus_tag="ICR95_28895"
     CDS             complement(1660..2538)
                     /locus_tag="ICR95_RS28895"
                     /old_locus_tag="ICR95_28895"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_205664587.1"
                     /translation="METENILDITFDRMTVMGDLGDNRVSNLIFLIKRFSITRENRIHI
                     SDSRYHDRVQGKFLDDLVYFEYDRLKAKAYNTRNFRIEFNPNKLNQEQIEWLQQNVISM
                     LSDVGITRLDLAFDVDFKLSDYQFISIGNAREKSTVEFRNSLSALETKYIGKRESDKMI
                     RLYDKKVEQMKQGNEVDLPHWWRLEFELKRETVDEFADVFETLQIKKPDLMEIEKVQDR
                     AMLYFLRDHPEEWSKLAKATRAKYRKMLREIKNKNMTPLFKKELKKKKAELMAQLDGWF
                     KNQYIVFESNN"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(2749..2973)
                     /locus_tag="ICR95_RS28900"
                     /old_locus_tag="ICR95_28900"
     CDS             complement(2749..2973)
                     /locus_tag="ICR95_RS28900"
                     /old_locus_tag="ICR95_28900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_205664583.1"
                     /translation="MVKKARTYRLTPEAIEKIETLVVLYNEMNQTDFKTIRISQADVLE
                     KIIRDHYDQLSMDIEKSVLDDAKEKAANK"
                     /name="hypothetical protein"
     gene            complement(3535..4098)
                     /locus_tag="ICR95_RS28905"
                     /old_locus_tag="ICR95_28905"
     CDS             complement(3535..4098)
                     /locus_tag="ICR95_RS28905"
                     /old_locus_tag="ICR95_28905"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_205664584.1"
                     /translation="MLIPISQLVKELKLKPHQLHKWEERGWLGFDPVLKDPDNNGQRIY
                     NEYQVERIYFIHEVIEEQKKQGIHRTNFKEMEEKLFEKFGGEVKRIENEVVEVLPASIE
                     AFQQLLVQQNKQINALTDMVQELQKKELQSPNDHTSDFEEMKKQLRLSEEREDKLLTLI
                     EKLQEDVQSLKEMVPKKSRWKFWG"
                     /name="MerR family transcriptional regulator"
ORIGIN
        1 caaatcttgg agtaaagctc tcatctttta cagtcacgaa ccttggtgta aactctcttt
       61 cattctgttt ttgtcttttg ataaactcct gatcaagcac ctcttttgta tattcatttc
      121 ctagctgttt cccactaata aaacgagtca tctccttcgt tttaaaataa taatgtccct
      181 tttgaatttt actatctatg tcgagttttg gatcttttaa gaaagtctgc aactctttaa
      241 aactagcaca ctttactttc gttagatcaa tagcagcacg caaatgagtt ttaaatggta
      301 gcttaccttt gaccattatg tgatactcag ctaacgtctt atttatttgt tttttctttt
      361 ccgtgacgat tgaaaggcca ttttccttgc aaatggcatc gttctgacgt tgccattcga
      421 aataatgttt ccttgtcaca ctgagcttct ttccatcttc gattgatgga gaattaagaa
      481 tgatatgatt atgaatatga cctttatcgg tatgtgtata aacaacagct tccctcttcg
      541 gatgcattct tttcaccaat tcaagcccta actcattcgc tttttctggg gatacttctc
      601 ctggcttaaa agattgaatg aagtggtgtc cctctacccc accagtctta ttgaacattt
      661 cccgtgttaa ttttatttgt ttctgtacac tcttcctgtc atcagcaatc aaattatgag
      721 aagaggtgac tccagcgcgt ttttcacagt aatttattaa tctatttact tgctttgtag
      781 cagagtgctt aattgttgcc attgttttag cacctcttcc ttcaagtcct gttgctcctt
      841 aatcattcca tgtaacgttt tattcaaacg ttctgcatgt tcttctttta aacctttctc
      901 gttcaaatgc tttgcaattt gatttaaatt atttcccatc ttgtttaaat cagtaccaac
      961 ttttctaata tcggttagca tttgcaggcc tactgactta tcaataacag gtgcctttaa
     1021 tcgagcattc ataatcaatt tccgaatcaa ttctgttttt ttcatgttat gttgttgcat
     1081 taatcgatag agcttatcct cttctacatc atttaagcgt atcgctggaa ttgcatgtgt
     1141 tcttcccatt gaaatccctc caaaaatcaa aacctgcgtc taaactatca accgtcattc
     1201 gtcagttccc agtaggtaat ttagttataa aaaacatcct gacgtctgtt atttataact
     1261 aaattatagc attaattacc atataaaact atggtaattt gctacgattc gcgttacaaa
     1321 tgcgatgcaa aagtgttgct tttttgcgca ttttaggacg cgaatcgcct tctggtcaca
     1381 ttcctcactt cgctcgataa ttagacttgg caagcaccgt atttgcgatg caaatacccg
     1441 tttcgctatg cttcacttgc ttgaaaattc tcgcgttgcg aaaattttga aaaacctaaa
     1501 aaaagaagaa caaagcttac gcaatgttct tcttttttct ttaacccaat tggattaaag
     1561 aaaaaagagg gtgtcacctt ttcgtgacac cctctttttt ttatttataa agcataacac
     1621 ctagcctata tgaatatttt tttgtataat ataagcacct tagttatttg attcaaaaac
     1681 gatatattga tttttgaacc atccgtcgag ttgtgccatc aactcggctt ttttcttttt
     1741 caattccttt ttgaaaagag gagtcatatt tttgtttttt atttctctta gcatttttct
     1801 atattttgct ctagtagcct tcgcaagttt cgaccattct tctgggtgat cgcggagaaa
     1861 gtacaacata gccctatctt gtactttttc aatctccatc aaatcaggtt tttttatctg
     1921 tagagtttcg aaaacatccg cgaactcatc gactgtttct ctctttaatt caaactctaa
     1981 tctccaccaa tgaggtagat ccacctcatt cccctgtttc atttgttcaa cttttttatc
     2041 atacaaacga atcatcttgt cactttctcg tttgcctatg tacttggttt ctaatgcact
     2101 taaagaattt ctaaattcga ccgtcgactt ctctctagca tttccgatac ttataaactg
     2161 ataatcactt aatttaaaat caacatcgaa agctaaatca agacgtgtta tccctacatc
     2221 actcaacata gaaatgacat tctgctgaag ccattcgatt tgttcttggt tcaatttatt
     2281 agggttgaat tctattcgga aatttcttgt attgtatgct tttgctttca atctatcata
     2341 ttcaaagtaa accaagtcat ctaaaaattt gccttgtaca cggtcatgat atcttgaatc
     2401 agatatatga atccgatttt ccctagttat agaaaatctc tttattaaaa aaattaaatt
     2461 agagactctg ttatctccca aatcacccat gaccgtcatg cggtcgaagg ttatatctaa
     2521 tatattttca gtttccaacc tctcttaccc ccctaaaaat tcttaaaaaa acccctaaaa
     2581 aatttccaaa aaatttccaa aaaagtttga agcactttta aaaaccttca aacccttgtc
     2641 gctctaagct ccaagtgcgt ttttgggtgt ggaaaaggtt caaagtttag cccttaaacc
     2701 ggctattaga agggagccgg ttgattttga atgaaaatat ccccctaatt acttgttagc
     2761 agctttttct ttcgcgtcat caagaacaga tttttctata tccatagaca actgatcgta
     2821 atgatctcta atgatttttt ctaagacatc agcctgagaa atcctgatag ttttaaaatc
     2881 cgtttgattc atttcgttat ataagacaac tagagtttct atcttttcga ttgcctctgg
     2941 tgtcaatcta taagtacgag cttttttgac catggtaaat cctcccaaag tttaataaac
     3001 ccattgtaac aatatttgaa taagtgtgca atacatttta aactccttgt attatagaat
     3061 gattacaaca agaaacagag gtaggctcaa gcatacctct gtttcttaag actatttaac
     3121 ctttaaaata actagcgccc tttcggttgc tctagatgtt atttgattta ccaaccttac
     3181 ggtgcaaaac caaataacat cttaccgaag tacctacata atatctactt tgtttagtaa
     3241 tcagtattta cttcgttagt gctaccgcat tagtacaagc tttatgtcaa taatgacgga
     3301 attcgtggat tctgtaaaca gagtaggcta aggcgatgta agggtgtgga aaaggttcaa
     3361 agtttagacc ttttaacata ccaagagggt cttggtgagc tgaatcagtc cactcgctat
     3421 agacagacgc catacaaggc ccatatagcc ccctaaggag gtttcccctt ctctttacct
     3481 tctgttccct tcacagcaac gaaaacgcct taaaagcact aaaaaaggat atatttaacc
     3541 ccaaaacttc caacgcgatt tctttggtac catttctttt agagactgca catcctcttg
     3601 taatttttca ataagggtaa gaagtttatc ctccctttct tcacttaacc ttaattgttt
     3661 tttcatttcc tcaaaatctg atgtgtggtc attcggagat tgaagttctt ttttctgcaa
     3721 ttcctggacc atgtctgtca aagcatttat ttgtttattc tgttgaacca ataattgctg
     3781 aaatgcttca atagatgcag gaagaacctc tacgacttca ttttctatac gctttacttc
     3841 tccaccaaat ttctcaaata gcttttcttc catctcttta aaattcgttc gatgaatccc
     3901 ctgctttttt tgttcctcga tcacttcatg aataaaatat atccgttcaa cttgatattc
     3961 gttataaatg cgctgcccgt tgttatctgg atcttttaaa accggatcga aaccaagcca
     4021 tcctcgttct tcccatttat gtaattgatg tggttttaat ttcaattctt ttactagctg
     4081 gctaatcgga atcaacaaac taccacctcc aaaatagcct aaattaagct taaatatata
     4141 ccttaattta actatatttt aagcttaatt taggctcact tatccttgca tatcaatgtt
     4201 tatggatata ctttgacaca tttttttaaa aaaaggtagg cttaagccta ccagtatagc
     4261 cttaaaggct atttatctat agttcacggt ccaagtcctg acttttactt cgcttatcag
     4321 caaatctagg cgtaaaccct ttgctttttt ctatagtcg
//
