LOCUS       NZ_CP069413             4697 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia megaterium strain CDC 2008724129 plasmid pPmC-129-16,
            complete sequence.
ACCESSION   NZ_CP069413
VERSION     NZ_CP069413.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16083678
            Assembly: GCF_017086545.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Shwed,P.S., Crosthwait,J., Weedmark,K., Hoover,E. and Dussault,F.
  TITLE     Complete Genome Sequences of Priestia megaterium Type and Clinical
            Strains Feature Complex Plasmid Arrays
  JOURNAL   Microbiol Resour Announc 10 (27), e0040321 (2021)
   PUBMED   34236233
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Shwed,P., Crosthwait,J., Weedmark,K. and Dussault,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Biotechnology Lab, PS-Biotech Lab, 251 Sir
            Frederick Banting, Ottawa K1A0K9, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017086545.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 06:19:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,820
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 199
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 148
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 24 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 25 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069413.1.
            Bacteria and source DNA available from CDC; Dr. C. Beesley.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Priestia megaterium"
                     /mol_type="genomic DNA"
                     /strain="CDC 2008724129"
                     /host="Homo sapiens"
                     /db_xref="taxon:1404"
                     /plasmid="pPmC-129-16"
                     /geo_loc_name="USA: Rhode Island"
                     /collection_date="2005/2006"
     gene            join(4660..4697,1..160)
                     /locus_tag="ICR95_RS28860"
                     /old_locus_tag="ICR95_28860"
     CDS             join(4660..4697,1..160)
                     /locus_tag="ICR95_RS28860"
                     /old_locus_tag="ICR95_28860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048558833.1"
                     /translation="MKVKQLVEKLEKLDQEGELMLFMRDRKRPHLGSGTLIPIKDIGVF
                     TSVDQDSNEGKYALEIKKGE"
                     /name="hypothetical protein"
     gene            complement(174..731)
                     /locus_tag="ICR95_RS28865"
                     /old_locus_tag="ICR95_28865"
     CDS             complement(174..731)
                     /locus_tag="ICR95_RS28865"
                     /old_locus_tag="ICR95_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016125306.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_048558832.1"
                     /translation="MPAEVRKLDEQRKINVELTPEQLQMLQRLIDLEQQENKDKEQFEE
                     KKKNHNFIQLYRDNMPELRWLMANHNFASSLLFFILEHMDNRNALACSYAVFEDYFGKS
                     RSTVYRAVKLLEENGFLNVLKMGTSNVYVVNEDLAWTDGNDKKKFAKYDGKILVSKKEN
                     KDFEYKSQFDKFKALRERENLK"
                     /name="replication/maintenance protein RepL"
     gene            1759..2322
                     /locus_tag="ICR95_RS28870"
                     /old_locus_tag="ICR95_28870"
     CDS             1759..2322
                     /locus_tag="ICR95_RS28870"
                     /old_locus_tag="ICR95_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002078386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_002019922.1"
                     /translation="MEVIMSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVS
                     KGGLLYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLEN
                     NNLNSALMAAMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFG
                     MAPLNEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            2322..2699
                     /gene="qacH"
                     /locus_tag="ICR95_RS28875"
                     /old_locus_tag="ICR95_28875"
     ARG             2323..2696
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3314..4660
                     /gene="mobV"
                     /locus_tag="ICR95_RS28880"
                     /old_locus_tag="ICR95_28880"
     CDS             3314..4660
                     /gene="mobV"
                     /locus_tag="ICR95_RS28880"
                     /old_locus_tag="ICR95_28880"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_053082646.1"
                     /translation="MSYSIIRMQKMKSNGIKGIQFHNQRERESETNPDINKADSHLNYD
                     LINGQRQIDYNEKISQVIEDNVTSGKKIRKDAVKLAEFLITSDKEFFDKISPKEQKRYF
                     ETAHEFLADRYGEKNLIYATVHYDEKTPHMHVGFVPVTEDGRLSAKDFFGQKKQLVSLQ
                     DDFNQYLKQNDFNLERGVSSSRKHVETAKYKAQTFQSMEKEAQEKYERTMGHIQEIDDK
                     SKSIANIEAKKVLGLVGMKEQDYKSLVDYATNGVAYQLQAENLQKELEKTQKEVTQLKA
                     DMQIGQDKIRYYYKDIEENLDSLAEKKALQKVKQTDIVKNYGDLIEKYNGLVNKYNDQL
                     KEKKELKQQVDTLKIENRSYQNENRELKLENGKLKGKLVQISKEFSVFKERVGKVLHAQ
                     LDRVKTFLRMNDVDRSTIKFLDDRQDKLVQDSLEKMEKPQRQKGMEMER"
                     /name="mobV"
     ARG             2323..2699
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
     Typing          complement(175..731)
                     /name="rep_cluster_1925"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.818"
                     /coverage="100.0"
                     /evalue="1.87e-127"
ORIGIN
        1 agaccaagag ggagaattaa tgctttttat gagggataga aagagacctc atttaggttc
       61 tggaactctt attccaataa aagatattgg tgtttttaca agtgttgacc aagattcaaa
      121 tgagggcaaa tatgctcttg aaataaagaa aggcgagtag ttatctactc gctttatttt
      181 aagttttcac gttctctaag ggctttaaat ttatcaaatt ggcttttgta ttcaaagtcc
      241 ttgttttcct ttttgctgac aaggatttta ccatcgtatt tagcaaattt ctttttgtca
      301 ttgccatctg tccaagcaag gtcttcgttc actacataga cgtttgatgt tcccattttt
      361 aaaacattta aaaatccatt ctcctctaaa agctttactg ctctgtaaac tgttgacctt
      421 gatttgccaa aataatcttc aaaaactgca taagaacaag ctaaagcatt tctgttatcc
      481 atgtgttcca agataaaaaa taataaagaa cttgcgaagt tatgatttgc cattaaccat
      541 cttaattctg gcatgttgtc acgataaagc tggatgaaat tatgattttt ctttttctct
      601 tcaaattgct ctttgtcttt gttttcttgc tgttcaagat caataagtct ttgaagcatt
      661 tgaagttgtt caggggttaa ctcaacatta atttttctct gctcgtccaa tttacgcacc
      721 tctgctggca tagaatcctt ctctcccttg ttatgtatca tcagctgtga tacctatatg
      781 tataatttat gacacatcat aggtcaaaaa tgaactatag tcaagcaaaa aatgacacat
      841 cttaattcaa ctatgtgtca caagctatga cacatatacg tgtcacaagc tatgacacat
      901 taccttaact aaaacattga tatatcaaca ttttttctgt gttcccttct atatctccta
      961 aagggctttt ctccacgcaa ggttcgggta gtatttttcg gacagaccac cgaaaaatct
     1021 ccaccctttt ctccttgcat tccgaaattt tggcggtgct gtactttcgc caagccgacg
     1081 tacctaaaac gaggaattca ggaaatcggc ttgctcgagc cattataccg ccaaaaccgc
     1141 cagagcttcc gttcataaat tttctctttt gccttatgga tctacgcaat atgggggctt
     1201 cccccaaacc cccttgccag cctccaccct aaaacaaggg gagaattgat ggtgttcgaa
     1261 taaaaatctt tgatatttag aaatctaatt ttaaagaagg gagttgagct ttcatgaata
     1321 gtaacagtag cattaataaa agtaatttaa atttattagc cgtagtagca ctaggattga
     1381 cgacaattta caccgcaata ttagcgactc aattaatgtc cacaaaacat aaagttgatt
     1441 atttatatta caaagaacat tttcaaaaaa ataataaggg gaataataat taatcgctaa
     1501 aacaatgaga ggcgctctag aaggggtaga gtcctgaaaa tgcggattta caacggtaat
     1561 gtaattttca cccataaata aagccgattt taagaaatcg actgttgaac taaagctccc
     1621 tttactttaa gtacatttct tcacaatctt tagattaatt tattggatta tttttatgtg
     1681 aaagagtgat attaaataag agctttaatt cgattgacaa aaaaaccgtc cagaaggtat
     1741 attatatgta tctaagttat ggaggttatt atgtctattt caaagcatgc taataagaaa
     1801 aaaaatatat tgattgctgc atctgaaatt gtaaaagaag aaggagttgt caaactcaca
     1861 ttagaggcag tcgcccaaag agctggcgtg agtaaaggtg gattattata ccattttcct
     1921 agcaaggaag cattaattaa aggaatggtg gaagattgga caaataatta tttcgaatgc
     1981 attaatacgt tagttaataa cgacgatgat aacgcaatag gaaaatggaa tagggcttat
     2041 cttaaatcta cattttccga tttagaaaac aataatctaa attccgcact aatggctgct
     2101 atgtttatta atccagattt attagatgaa ttccggcaga gatacgatat tttgcataca
     2161 aagttaataa ctgatggaat tgatcctgtc aaaataacaa ttacaagatt atccatagat
     2221 ggtctttggt tttcagaaat atttggaatg gcaccattaa atgaagaact aaaaacacaa
     2281 gtttttgatg aactaataaa catgattcag gaggacgaat aatgtcatat ctatatttag
     2341 cacttgcaat agtgggggag attattggct cttccctatt aaaagcatct gaaggctttt
     2401 caaaactcta tcctacaata ggggtaataa tagctttcgt tggttgtttt ttctttttat
     2461 ccttgtcgct aaaaacaatc cccttaaaca ctgcatatgc attatgggca ggtttaggac
     2521 ttgttttgac tacagtaatt tcagttttag tttggaagga aaaacttaac atggctagca
     2581 ttgcaggaat aacactgatt cttgtaggag ttgtaatatt gaatttgttt ggaccaggac
     2641 atggagaatc tggtcatgaa gcaagcgaaa ctattacaat gaatgagaca atggaataac
     2701 acataaaaaa gtccttctta ataaagagaa ggacttttta gcttaattaa gggtattttt
     2761 tgaagtcctt attcaacaaa cctgcgtacg ttagtttaag tgtatcactt cacaattcca
     2821 atagagtaga gtttattgag acataatgaa attagcgttt taaatgactt ctgactgtct
     2881 cataagactt gtatcaaatg cttaccgttg taaatccgca tttccctgac gctaccccta
     2941 gaagcgtctt ttttgctttt taagggtttt tactaggggc aacctcttac aaaggcgaga
     3001 gaggcccgta tggcgtctgt caacggtacc ggaccgaagg gaggataagg agttgataga
     3061 ctcacttcac caagaccctc ttggtgtctc aaaaggcaac accttttgag ctgcctaccg
     3121 gcgagggagc ccctcaagga ttgaggggtt ggggagttcg attaaggggg tctggggaag
     3181 agttccccag tgggtttggg caaagcccaa ggtttggctt ttagcgtcgc agaccacatg
     3241 aaactttgcg tgcaaagtgt agtgtgttac actttctatt aatctatcga ttttcagaaa
     3301 gggtgcgtac cccatgagtt actccattat ccgaatgcag aaaatgaaat caaatgggat
     3361 aaaagggatt caatttcaca accaaagaga gagggaaagt gaaacgaatc ccgacatcaa
     3421 taaagctgat tctcatctga attatgactt gataaacggt caaaggcaga ttgattacaa
     3481 cgagaaaatt agccaagtaa ttgaggacaa tgtgacctct ggaaaaaaga tccgtaaaga
     3541 tgcagttaag ctggctgaat tcttaattac ctctgacaag gaattcttcg ataaaatttc
     3601 acccaaagag cagaaaaggt attttgaaac ggctcatgag ttccttgctg atcgatacgg
     3661 tgaaaagaat ttaatctatg cgactgttca ctatgacgag aaaacccctc acatgcacgt
     3721 aggcttcgtt ccagtaacgg aggatggccg actatcggct aaagatttct ttggccagaa
     3781 aaagcaactg gtgagtcttc aggacgattt taatcagtac ctgaagcaaa atgactttaa
     3841 tttggaaaga ggggtctcgt cgagccgtaa acacgtggag acggctaaat ataaggctca
     3901 aacgtttcaa agcatggaga aggaagccca agagaaatat gagcggacaa tggggcatat
     3961 tcaagagatt gatgataaat cgaaatcgat tgcgaatatc gaagctaaaa aggttctagg
     4021 gcttgttgga atgaaggaac aagactacaa aagcctagtc gattatgcga ccaatggtgt
     4081 ggcttatcag ctacaagcag agaatctgca gaaagagttg gaaaagactc aaaaagaagt
     4141 tacgcagctg aaagctgata tgcaaattgg acaagataaa attcgctatt actacaaaga
     4201 tattgaagag aatttagact ccttagcaga gaaaaaagca ctgcaaaaag tgaagcaaac
     4261 agacattgta aagaactatg gagacttgat agaaaagtac aatggactcg ttaataaata
     4321 taatgaccag ttgaaggaga aaaaggagtt aaaacaacag gtggacactc taaaaattga
     4381 gaaccggtct taccaaaatg agaatcggga attgaaatta gagaacggaa aattgaaggg
     4441 aaaactcgtg cagatcagca aggagttttc tgtttttaaa gagagggtgg gcaaagtttt
     4501 acacgctcaa cttgaccgag taaaaacgtt cttgaggatg aatgatgtag accgaagtac
     4561 aatcaagttc ctggatgata ggcaagataa attagtccag gactctttag aaaaaatgga
     4621 gaagccacaa aggcaaaaag ggatggagat ggaacgctga tgaaggtgaa acaattggtt
     4681 gagaagttgg agaaact
//
