LOCUS       NZ_CP069410             7421 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia megaterium strain CDC 2008724129 plasmid pPmC-129-13,
            complete sequence.
ACCESSION   NZ_CP069410
VERSION     NZ_CP069410.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16083678
            Assembly: GCF_017086545.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Shwed,P.S., Crosthwait,J., Weedmark,K., Hoover,E. and Dussault,F.
  TITLE     Complete Genome Sequences of Priestia megaterium Type and Clinical
            Strains Feature Complex Plasmid Arrays
  JOURNAL   Microbiol Resour Announc 10 (27), e0040321 (2021)
   PUBMED   34236233
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Shwed,P., Crosthwait,J., Weedmark,K. and Dussault,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Biotechnology Lab, PS-Biotech Lab, 251 Sir
            Frederick Banting, Ottawa K1A0K9, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017086545.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 06:19:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,820
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 199
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 148
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 24 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 25 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069410.1.
            Bacteria and source DNA available from CDC; Dr. C. Beesley.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Priestia megaterium"
                     /mol_type="genomic DNA"
                     /strain="CDC 2008724129"
                     /host="Homo sapiens"
                     /db_xref="taxon:1404"
                     /plasmid="pPmC-129-13"
                     /geo_loc_name="USA: Rhode Island"
                     /collection_date="2005/2006"
     gene            complement(join(7188..7421,1..984))
                     /locus_tag="ICR95_RS28740"
                     /old_locus_tag="ICR95_28740"
     CDS             complement(join(7188..7421,1..984))
                     /locus_tag="ICR95_RS28740"
                     /old_locus_tag="ICR95_28740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_205663454.1"
                     /translation="MATIKLSTTKNANALLKYAEKRAEVANSLDCDVDYVRNQFKATRE
                     IWGKSGGIQAHHVIQSFKPDEVDPQKANEIGLKLAEKLAKGHEVAVYTHTDKDHIHNHI
                     VINAVNYEDGRKFHAHGQEAIDRFRDVSDELCKEHGLSIVEERSADVRYTLAEQSLLQK
                     GESSWKDEIRTAIDSSKEQTTSFEEFQERLKDKGVQATLRGKNITYEHLESNKKVRGSK
                     LGLAYEKETILDGFERQVTRERAAAGSAKRGVTTVTAVIPGDDSTSQSDRGLSLDVPER
                     KHEQRDDDATRARTDQTDEPRRTEGNEIDFADIDQQIRDRHLRAQDIYERNFGRDEDGH
                     QGIAQRDETESGVSEQRVPNKQSNDQGRTREDQQQPGEQVRHVEREGEIRTQRNREKQQ
                     DYDIER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(966..1319)
                     /gene="mobC"
                     /locus_tag="ICR95_RS28745"
                     /old_locus_tag="ICR95_28745"
     CDS             complement(966..1319)
                     /gene="mobC"
                     /locus_tag="ICR95_RS28745"
                     /old_locus_tag="ICR95_28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_012209755.1"
                     /translation="MSETKGERRNEPKQVKFRVTEEEFERLSLMADNVGMTVPAFVKAK
                     AQGTRVRQPKINREGALAIAKELRAVGTNVNQIARWCNARDIEQLSEQELERLVYNLDE
                     IKKGLAAAWQQLS"
                     /name="mobC"
     gene            2111..2977
                     /locus_tag="ICR95_RS28750"
                     /old_locus_tag="ICR95_28750"
     CDS             2111..2977
                     /locus_tag="ICR95_RS28750"
                     /old_locus_tag="ICR95_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010649677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="WP_047934313.1"
                     /translation="MFFHKKELQYHAKPDRPDPLFAKQLQEVLGGQFGEISVALQYLFQ
                     GWNVRGNGKYKDLLMDTGAEELAHIEMLATMIARLLDRAPVGDLEKAAQDPVIGAILGG
                     MNPQHAIVSGLGAMPADSVGNRWHAGYIAASGNLLADFRANLNAESQGRLQVVRLYEST
                     SDRGVKDMLSWLIARDTMHQNQWIAAIKELEEKENVVVPSTFPRHLEKREVSHVLFNFS
                     EGEKSATGRWASGPSMDGEGVFQYVQCPEGFGGKPKLKPAPPYIHDTPPQDLKRNDNFT
                     PPPPHLA"
                     /name="manganese catalase family protein"
     gene            complement(3158..3667)
                     /locus_tag="ICR95_RS28755"
                     /old_locus_tag="ICR95_28755"
     CDS             complement(3158..3667)
                     /locus_tag="ICR95_RS28755"
                     /old_locus_tag="ICR95_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016766106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="WP_205663453.1"
                     /translation="MLVKKMLNGIMMKEITIKELADRYDVSTRTIQSKIKKLGYEWDSK
                     ESIYRYVGEESEPLDVDFSTLISKNSKMPAKQQLANSEIAASVSYLNESESTNDSFEKA
                     STNDSKVDAIDLLLQHPKERSKQVYRGFYFDQDVLSIIDRVPKSYKSELVNEALRKVFK
                     EKGLLD"
                     /name="HTH domain-containing protein"
     gene            4038..4577
                     /locus_tag="ICR95_RS28760"
                     /old_locus_tag="ICR95_28760"
     CDS             4038..4577
                     /locus_tag="ICR95_RS28760"
                     /old_locus_tag="ICR95_28760"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="WP_205663452.1"
                     /translation="MSKTCIIGNGCAGKTTLGKKLSQDLSLPFFEMDEKLKFLKTTPFP
                     TRQQIRHFSRELNKLLDKDKWVIEGWGYKETYEERFKKADIIIFLDVPFQVCLERCHKR
                     LGSTNLEILKRGVTKLNIFDKHVRPLLLKLIQKYKNEPKKIILEIDQDFKYEDILRQIN
                     MNTVPINKKSEYQTII"
                     /name="shikimate kinase"
     gene            5077..5643
                     /locus_tag="ICR95_RS28765"
                     /old_locus_tag="ICR95_28765"
     CDS             5077..5643
                     /locus_tag="ICR95_RS28765"
                     /old_locus_tag="ICR95_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_233335540.1"
                     /translation="MYMEFYEKVDGLARAIRVTTSTAKKYYLLFEEHGYNFKRNQQGQL
                     LFSQDDINLFKELMILKNEKGMTVPKAVKEIVSGKVITDITDITDITVTTEDITVITSK
                     VTTVMTEIEELKKLVKDQNEIIEKQQENFKKQFLKQEAYIKDNLEQRDKTLVTTLREMQ
                     DSQKEIAATLSKEKDKKKWWQIWRS"
                     /name="DUF3967 domain-containing protein"
     gene            complement(5953..6576)
                     /locus_tag="ICR95_RS28770"
                     /old_locus_tag="ICR95_28770"
     CDS             complement(5953..6576)
                     /locus_tag="ICR95_RS28770"
                     /old_locus_tag="ICR95_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170182.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_233335541.1"
                     /translation="MKKRKFKINFDDAEQNARLRDLENEKEKLKEDYHGVDQDDVNKAI
                     EVLSKATGGKEIYVGTKRSPKSKVKFAQFIQDNWDYALDNDYFTDEQMLFLLRIQRYMQ
                     FKSNCIVDNIHSRSAVPLTQKAIAERLKTSAPKVSRIVNQLVDKGVIVKAQGQKTEGNN
                     ARTYALFINPNIIYSGERDNIETTLKALFANAKPLFKNFPVTLF"
                     /name="MarR family transcriptional regulator"
     gene            complement(6760..6966)
                     /locus_tag="ICR95_RS28775"
                     /old_locus_tag="ICR95_28775"
     CDS             complement(6760..6966)
                     /locus_tag="ICR95_RS28775"
                     /old_locus_tag="ICR95_28775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_153252747.1"
                     /translation="MTEKKTLKTTSIRLDEDELVMVQELINSFNDFNDFSFKPITQNDV
                     IRLALRRLYEKEIPVESRSVKNK"
                     /name="hypothetical protein"
     Typing          complement(2..984)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.72"
                     /coverage="100.0"
                     /evalue="9.83e-180"
ORIGIN
        1 tatgtcttgt gctctaagat ggcggtctct aatttgttga tcgatgtcag cgaagtcaat
       61 ctcatttccc tctgtacgtc ttggctcgtc tgtttgatct gttcgagctc ttgttgcatc
      121 atcatctctt tgttcatgtt ttctttcagg aacgtcgaga gatagccctc tgtcgctctg
      181 agacgtagaa tcatctcctg gtataactgc tgtaactgtt gtaactcctc tttttgctga
      241 acctgctgct gctctttctc ttgtaacttg tctttcaaag ccatctaaaa tcgtctcctt
      301 ttcatacgcc agccctaact tgctgccacg tactttttta ttcgattcta agtgctcata
      361 cgtgatgttt ttacctcgta aagtagcttg gacgcctttg tcttttaaac gttcctggaa
      421 ctcttcaaaa ctcgttgtct gctcttttga agagtcaatg gccgtccgaa tttcatcttt
      481 ccaactcgac tcaccttttt gaagcaagga ctgttcagct aacgtatagc gcacatcggc
      541 cgagcgctct tctacaatgg acaaaccatg ttctttacat aattcatcgc tcacgtcacg
      601 aaaccggtca atggcctctt ggccatgcgc atgaaacttt cgaccatcct catagttcac
      661 agcattaatc acgatgtggt tatgaatgtg atccttgtcc gtatgcgtgt agacggccac
      721 ctcgtggcct ttggctaatt tctcagctag ttttaaccca atttcattag ctttctgagg
      781 atctacttca tcaggtttaa atgattgaat gacatggtgg gcttgtatgc cgccactctt
      841 gccccatatt tcccttgtag ctttaaattg gttccgaaca tagtccacat cgcaatcaag
      901 cgaatttgcc acctcagcgc gcttctcagc gtacttcagt aacgcattgg catttttcgt
      961 cgtactcagc ttaattgttg ccatgctgca gccaatccct ttttaatttc gtcgagatta
     1021 tataccaatc tctcaagctc ctgttcactc aattgttcga tgtctcgtgc attacaccat
     1081 ctcgcaatct ggttcacatt ggtacccaca gcacgcaatt ccttcgcgat tgccagagca
     1141 ccctcacgat tgatcttcgg ctgtcgtacg cgggtaccct gtgcctttgc tttcacaaag
     1201 gcaggcaccg tcattcctac gttatccgcc atcagcgata gccgttcgaa ttcctcctca
     1261 gtgacacgga acttcacctg tttaggctcg ttcctacgct cgcctttcgt ctcgctcatt
     1321 cctttattcc tccttatgtt cacctactag atgtggtgaa cttggtgtaa aattccagct
     1381 ttatgcttcc ataatacacc aaacagtcgg aataaggcaa gctctacaat tggtacccaa
     1441 ttgagcttgg ttatgctgag cataacaatc cccccagccc ccttttcaag ggggagccaa
     1501 accgcggacg ctgtccacac ctgccaaagg aaagctcctt tggaatggct tctacaaaca
     1561 cccccaaccc cctcaacact ttgagggggc tccctcgccg gttggcgtca aacaaacgtg
     1621 gttgtttgag tgccaagagg gttcgggtgt agagacattg tcaattcctt aatgctccct
     1681 acggtccgct taccattgac aatgcagctt atctccccct ttgttttggg gtagaggctg
     1741 gcttcttatg tttttaagag tgttgctcac cttgaagtaa gatgagcact tttgggcggc
     1801 cgagctggcg gttcggcagg tatacttgcg caaacaagcc gattctcaaa ttcttcgttt
     1861 tgggtatgtc ggcttggcga gaaatacaaa cctgccgaaa gtctttgtac aaggagaaaa
     1921 gagtggagat tttttaagtg ggtttcttaa aaaatacgac tcgtaccttg tataaagact
     1981 agctattttt taggggaggt agaagatatt acagtttcta ttttataaat aaatttagac
     2041 acctatcctt atatagttga atagcctata aaagattatt ttgaataaaa agtagatagg
     2101 atgatttcta ttgttttttc ataaaaagga acttcaatac catgcaaaac cagaccgtcc
     2161 ggatccttta tttgcaaagc aactgcaaga agttttaggg gggcaatttg gtgaaatctc
     2221 tgtagcatta caatatctat ttcaaggatg gaatgtacga ggtaacggca aatataaaga
     2281 tttacttatg gatacaggag ccgaagaatt agctcatatc gaaatgttag ctacaatgat
     2341 cgcccgactt cttgatcgag cacctgttgg agatttagaa aaagcagctc aggatcctgt
     2401 cattggagcg attcttggag gcatgaatcc acaacatgct attgtttccg gacttggggc
     2461 catgccagca gatagtgtag gaaacagatg gcatgcaggt tatattgcag caagtggtaa
     2521 tctcctggca gattttcgcg ccaatttaaa tgcagagtca caaggtcgcc ttcaagttgt
     2581 acgtctctat gaatcaacta gtgatcgtgg agtgaaagat atgctatctt ggttgattgc
     2641 tcgggatacg atgcatcaaa atcaatggat tgcagcaatt aaggagttag aggaaaaaga
     2701 aaatgtagtt gttccaagta cgtttcctcg acacttagag aaacgggaag tatcccatgt
     2761 cctctttaat ttttctgaag gagaaaagag tgctactgga cgctgggcaa gtggtccaag
     2821 catggatgga gaaggtgtat ttcaatatgt tcaatgccca gaaggatttg gaggcaaacc
     2881 aaaattaaaa ccagctcccc catatattca tgatacacca cctcaagatt taaagagaaa
     2941 tgataacttt actccacctc ctccacattt agcttaaaaa gaataacatt taacaatttg
     3001 agcaaactac tcaagatgta taaaagcatc aaaactaact tctaattatg tcacagaaaa
     3061 aactactggt ccccgatgag ccggtagttt tttctgtttt ttaaggtgta tatgatatat
     3121 atttttagtt aacataaaac catttttcgg aacactttta gtctaataac cctttttcct
     3181 taaacacttt tctaagagct tcatttacta attcagattt ataactctta ggaactcgat
     3241 caatgatact taacacatcc tggtcaaaat aaaaccctct atacacttgt tttgagcgtt
     3301 cttttggatg ctgcagtaac aaatcaattg catctacttt gctatcattt gtactagctt
     3361 tttcaaagct gtcattagta ctttcacttt cattcagata gctaacacta gctgctattt
     3421 cgctattggc tagctgttgt tttgctggca ttttactgtt tttagagata agagtactaa
     3481 aatcaacatc taaaggttct gattcttctc caacatagcg ataaatactt tccttactgt
     3541 cccattcata gcctaatttc ttaatctttg attgaatggt acgagtactt acatcatacc
     3601 gatcagctaa ttctttaatg gtgatttctt tcatcataat tccatttaac atctttttta
     3661 ccagcatctt actagccccc attttcgttt taaaattata gtactagtat tcaaatgcaa
     3721 agtaatattt cctgctatta ctttgctaca aaatgaataa ttaagatcaa catgagcaaa
     3781 ctactcgaga agtcttttag tactccaatt acaacctaat atttatcagt agaaaaagcc
     3841 attttcccca aagatggctt tttcgccttt tagttaacat aaaacgtatt atcagaagta
     3901 aatatttaca gcaggattaa gcctgctgct attttatatt gactctagaa aaataataat
     3961 gtcccatata tggaacatat acataacatt gattaggaca gtgatttaac tgtcaattcc
     4021 aaagaggaga gtgaaacatg tctaagacat gtattattgg aaatggttgt gcagggaaga
     4081 ctactctagg gaaaaagtta agccaagatc tttcacttcc attctttgaa atggatgaaa
     4141 aactcaaatt tttaaaaaca actccttttc ctaccaggca acaaatcaga catttttcaa
     4201 gagaactaaa caaattatta gataaagata aatgggtaat agaaggttgg gggtataaag
     4261 aaacttacga agaaagattt aaaaaagcag atattattat ctttctagat gtacctttcc
     4321 aagtttgttt agaacgatgc cataaaagat taggttcaac caacttagaa attctaaaac
     4381 gaggcgtaac aaagttaaac atatttgata aacatgtacg tccattgcta ttaaagctaa
     4441 tccagaaata taaaaatgaa cctaaaaaaa taatcctaga gattgatcag gatttcaaat
     4501 atgaggatat attgagacaa attaacatga atacagtgcc tattaataag aaatctgaat
     4561 atcaaacaat aatttgagca aactactcaa gaaactgata aggtttcaac cctttggata
     4621 aatgtagtag aaaaactccc ttaacgctct aagggagttt ttctgttttt gaactgtata
     4681 ctctatacaa aggtagttga cataatgtct ctttccggaa cacaacccaa aaaagaaccc
     4741 ttgtcgcaga acggttccgc cgagggttct ttttccggag tatgcaactt tttatacatc
     4801 cttgatatga cggagttccc cttataggtg gtcggagaaa tgatgaataa aaagtgcacc
     4861 ttttattcat atgcaagccc ttataaaaaa agcatgtatc ggaaaaagta tgatacaata
     4921 acccctggtc tattttttac aaatttcaac gtagcaaaaa gagcaacctt tcgtccactg
     4981 cagaggttgt ttctttattt tctttaagac actaatatct tatttgaggg tgttataata
     5041 gttatgattc tgaataaatt ggaggttata actgttatgt acatggaatt ctatgaaaaa
     5101 gtagatggat tagcaagagc tattagagtc acaacttcaa ctgcaaagaa atattattta
     5161 ctttttgaag agcatggtta taactttaaa cgtaatcagc aaggacaact cttgttttca
     5221 caagatgata taaatttatt taaagaatta atgattttaa aaaatgaaaa aggtatgaca
     5281 gttccaaagg ctgtaaagga gattgtaagt ggaaaagtta taactgacat aacagatata
     5341 acagacataa ctgttacaac agaagatata acggttataa ctagcaaagt tacaactgtt
     5401 atgactgaga tagaagagtt aaaaaagtta gtaaaagatc aaaatgaaat tatagagaaa
     5461 caacaagaaa attttaaaaa gcaattttta aaacaagaag cctacataaa agacaatcta
     5521 gaacaaagag ataaaacgtt agttacaaca ttaagagaaa tgcaagattc acagaaagaa
     5581 atagctgcta ctttatccaa agaaaaagat aagaaaaaat ggtggcaaat atggcgaagt
     5641 taaaccactc aatcaatttg agtggttttt tgtatattgc aaaaatgcaa cgcataacag
     5701 ttataactac tcggccgtct taagacggct tttttagcct tcaggctata tttttttcaa
     5761 cgaaaaaaat acgaaaagac tcttgcaatt ctattaacga attgctatat aaatagagag
     5821 tgagagtaaa ataaccatta aggtagtaat aaaccctttt ataccaaggc tttataccgg
     5881 atttcgtagt taccgaatta gataactata gttaccgaat tagataacta cgaaatcata
     5941 aaaaagtaac ccttagaaaa gggttactgg aaaattttta aataaaggct tagcattagc
     6001 aaaaagtgct tttaaagttg tttctatatt gtctcgttct ccactataaa taatattcgg
     6061 attaataaac aaggcatatg tacgggcatt attgccttct gttttctgac cttgagcttt
     6121 aacaattacg cctttatcaa ctagctgatt tacaattcta ctaaccttag gcgcactagt
     6181 ttttaatctt tcagcaatag ccttctgagt caatggaact gcacttctag agtgaatatt
     6241 atctacaatg cagttacttt taaattgcat atatctttga atacgaagta agaagagcat
     6301 ctgttcatca gtaaaataat cattgtctaa agcataatcc caattatcct gaataaattg
     6361 agcaaatttc acttttgatt ttggagaacg tttagttcca acgtaaatct cctttcctcc
     6421 agtcgcttta cttaatactt ctatagcctt gttaacatca tcctgatcaa caccatgata
     6481 gtcttctttt aatttttctt tttcattttc taaatctctt aacctagcat tttgttcggc
     6541 atcatcaaaa ttaattttga attttctttt tttcaattgt taattcctct cctctagcag
     6601 gaattaaccg tcctgtgtta aaataaaatt aattaaatag gttgcacaac aacaagaggt
     6661 taatccctcg aaatgttttg ttaaaagtcc tctggcccgc taaaactaga ggactttctt
     6721 atttttaatt ttaaatcata tagatcttta tttcattgat tatttattct ttacacttct
     6781 tgattctaca ggtatttctt tctcatataa ccttcttaag gccaaacgta taacatcatt
     6841 ttgagtaata ggtttaaaac taaaatcatt aaaatcatta aatgagttaa ttaactcctg
     6901 aaccattact aattcgtctt cgtcaagcct tatggaagta gtttttaacg tctttttttc
     6961 tgtcatgtca atacctccaa cttcttctat atacaacatt atacaattca atttaatcaa
     7021 tcgcaataca atgtaatcta ttagattata aaagaagatg ttatcctaac tctataccaa
     7081 acaacccaaa gaaccacttg aacaagaaga acataggcac cataaagacg gccgccccag
     7141 ctccatactt cacccaatct aaccagccat taaacaccac gcccaactta gcgttcgata
     7201 tcatagtctt gctgtttttc tcgatttcgc tgagttctga tttcgccttc tcgctcaaca
     7261 tgtcgaactt gctccccagg ttgttgttga tcttctcgag ttcgtccttg atcatttgat
     7321 tgtttgtttg gaactcgttg ttcagaaact cctgattctg tttcatctcg ctgtgcaata
     7381 ccttgatggc cgtcttcatc tcggccaaag tttcgttcgt a
//
