LOCUS       NZ_CP069409             8052 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia megaterium strain CDC 2008724129 plasmid pPmC-129-12,
            complete sequence.
ACCESSION   NZ_CP069409
VERSION     NZ_CP069409.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16083678
            Assembly: GCF_017086545.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Shwed,P.S., Crosthwait,J., Weedmark,K., Hoover,E. and Dussault,F.
  TITLE     Complete Genome Sequences of Priestia megaterium Type and Clinical
            Strains Feature Complex Plasmid Arrays
  JOURNAL   Microbiol Resour Announc 10 (27), e0040321 (2021)
   PUBMED   34236233
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Shwed,P., Crosthwait,J., Weedmark,K. and Dussault,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Biotechnology Lab, PS-Biotech Lab, 251 Sir
            Frederick Banting, Ottawa K1A0K9, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017086545.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 06:19:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,820
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 199
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 148
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 24 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 25 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069409.1.
            Bacteria and source DNA available from CDC; Dr. C. Beesley.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Priestia megaterium"
                     /mol_type="genomic DNA"
                     /strain="CDC 2008724129"
                     /host="Homo sapiens"
                     /db_xref="taxon:1404"
                     /plasmid="pPmC-129-12"
                     /geo_loc_name="USA: Rhode Island"
                     /collection_date="2005/2006"
     gene            complement(118..798)
                     /locus_tag="ICR95_RS28695"
                     /old_locus_tag="ICR95_28695"
     CDS             complement(118..798)
                     /locus_tag="ICR95_RS28695"
                     /old_locus_tag="ICR95_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014462146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_205664577.1"
                     /translation="MNEKQDTINAEEMAKRLNTTSSNIRKWCIELEKHGYEFIKDNRKK
                     RYYSVTDQSLLSNLKYAIQEKKLSLEIAAMTVIKGFDRNRSSSGTQEEHLKKEENNREL
                     SLTQEHNNHRTDAVLNTMKNQFEQQQSVNNKLIEMLDHYKAAFEESENRRRSEQEHMNK
                     QLKELEERLAVRISKQISNRDKEMMEYIRSSQKQQQEQYLEIAATQQNEEEQKKGFFAR
                     LLGK"
                     /name="hypothetical protein"
     gene            1557..1985
                     /locus_tag="ICR95_RS28700"
                     /old_locus_tag="ICR95_28700"
     CDS             1557..1985
                     /locus_tag="ICR95_RS28700"
                     /old_locus_tag="ICR95_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350989.1"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="WP_034656318.1"
                     /translation="MAVVKATTSDIALLARLLRAEGEGEGVQGMLLIGNVGINRIRANC
                     SDFKGLRTIPQMIYQPHAFEATQKGYFYQRARESEKRLARRSVRGERSWPAKYSLWYFR
                     PPQKCPSTWYKQPLVARYKLHCFYQPKAETCENIYNTF"
                     /name="cell wall hydrolase"
     gene            complement(2195..2566)
                     /locus_tag="ICR95_RS28705"
                     /old_locus_tag="ICR95_28705"
     CDS             complement(2195..2566)
                     /locus_tag="ICR95_RS28705"
                     /old_locus_tag="ICR95_28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_234943166.1"
                     /translation="MVWGVLIINSITGNRVTGTINFRGTLIPINGYWDESAKQISFDSP
                     YATYSGNLTMFDDSTIRIRHLILTGRVIMKPPSLLAGRNGTWIATTDTTLIGPAVTNSD
                     LPPVGAFLTSNILHSGLER"
                     /name="hypothetical protein"
     gene            complement(3051..3476)
                     /locus_tag="ICR95_RS28710"
                     /old_locus_tag="ICR95_28710"
     CDS             complement(3051..3476)
                     /locus_tag="ICR95_RS28710"
                     /old_locus_tag="ICR95_28710"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_338127782.1"
                     /translation="MEESYVSKEPDYVKIYLDNILQINNLSSGIQKTLNVLLKRMAYDN
                     IVVLNAYIKKQMAEELGFNTVQSLNNNINKLVKEEIMIRKGTGTYEMNPFLFGRGSWDN
                     IKKIRFQVIFEEGKISQKADFDYKDEVAISHEEKTTD"
                     /name="replication/maintenance protein RepL"
     gene            complement(<3502..3708)
                     /locus_tag="ICR95_RS28715"
                     /old_locus_tag="ICR95_28715"
                     /pseudo=""
     CDS             complement(<3502..3708)
                     /locus_tag="ICR95_RS28715"
                     /old_locus_tag="ICR95_28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /name="spore coat protein"
     gene            complement(3864..5846)
                     /gene="mobQ"
                     /locus_tag="ICR95_RS28720"
                     /old_locus_tag="ICR95_28720"
     CDS             complement(3864..5846)
                     /gene="mobQ"
                     /locus_tag="ICR95_RS28720"
                     /old_locus_tag="ICR95_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_205664578.1"
                     /translation="MAIYHLSMQIISRSKGQSAVAAAAYRSGEKLHDERTDEQKFYARH
                     AQPETMILTPTNAPEWMKDRNRLWNEVEKVEKRKDSQLARELNIALPIELNHDQQKELI
                     RSFAQNEFVQKGMVADIAIHRDDTNNPHAHIMLTMRNLNQDGFSKKNRDWNADFANVKE
                     NNRGYVKNSEGCLSIREQWASYANKALEQAKINERITHLSHEKRGLETLPMVHLGHVAH
                     DMEKKGKQSERGQVNRERQEYNQVVIDLQAYRRQKEAHVQKMKEKKKQFSFSTDVEKTY
                     IQKAASLLDQKVISLDDISRRQKELRKMADRHDPIERHFHVQQQQFLNVNNYYDRLRAL
                     EREIKQNEEKVDELKGSLNPFKLKENNMMKRRYLDKISDLDSNKANYESSIEEYKESLR
                     FSNEEEFYQRYQEFSQQKENRMHQIKYEKQQIRVETKTLSQAEEAIKTAKIREVSSHYP
                     DLKTAGNYLSYENALKLEKHIDMAKKTPVTLEVIKKAIKNQKDDLYKLRKDLKIFQIEK
                     DHLDKMKSQVTNLEKSKNELDKLNNNPGEAAKRLVSKKTRQHYEELQHEVNHYRENLKE
                     KGYKGLDDLNKRQSEIRGLEQNVIPTIEKQIKTKENIIGTVSGIFNAIQQATRSEEQAQ
                     QRQHLRRFRSSSINRNKSQGPDLSR"
                     /name="mobQ"
     gene            6223..6534
                     /locus_tag="ICR95_RS28725"
                     /old_locus_tag="ICR95_28725"
     CDS             6223..6534
                     /locus_tag="ICR95_RS28725"
                     /old_locus_tag="ICR95_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_205664576.1"
                     /translation="MTKKRTDEEILQELEEKIEKMKAKKQQVEARKKEKERKERTRRLI
                     QVGAIFEKYFEIRSEEEAEKIAKALQSYVGKNKDKILHHDVVVKEKTKAAAEVAVSEE"
                     /name="hypothetical protein"
     gene            6554..6967
                     /locus_tag="ICR95_RS28730"
                     /old_locus_tag="ICR95_28730"
     CDS             6554..6967
                     /locus_tag="ICR95_RS28730"
                     /old_locus_tag="ICR95_28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014462149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_234943165.1"
                     /translation="MKCLYCDSDNNSMDKGCSVCGAPLNIKRPQLKEYVSLQDSALSFQ
                     ELQSFHTYDLLILLRLVREERSKSYNLMRTVQKAPEGAEIDKSVIEFGESEYRLYTARM
                     KLIEGLLIDRMGYKPKRVDDKLLAALKHRMGSK"
                     /name="hypothetical protein"
     gene            7158..7850
                     /locus_tag="ICR95_RS28735"
                     /old_locus_tag="ICR95_28735"
     CDS             7158..7850
                     /locus_tag="ICR95_RS28735"
                     /old_locus_tag="ICR95_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048019661.1"
                     /translation="MKKVSDLQNSLNEAEQKARLRDFEKDQNSFQKAEDLKKSLLEQLK
                     EITGEDHFVGTSKDPFAGEPFNQTMHRNLDLLNQIDYLTQAEGDFLFRIQPYLEFKSNA
                     IIRREDKFKKNKKSTVDLEDDYELVQSATVSDIAEMIGKTRANTSKIMQSLKEKEILLN
                     PEGAGQVIDNGRTVSPRTWIVNPYIMICAPRKNKVQLDRLTMRLFQNSLKNLKDQNGKK
                     VKLPARFF"
                     /name="hypothetical protein"
     Typing          complement(3877..5846)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.714"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttcgctaata gaattgcaag ggtcttttaa tacttttttc gttttaaaaa attagccatt
       61 aaggcaatca ggccgcctca aggcggtccc tacagataac tattcaaacg agttatttta
      121 cttgcctaat aatcgagcaa agaatccttt tttttgttct tcttcatttt gttgtgtagc
      181 tgctatttct aaatattgct cttgctgttg tttttgagaa gaacgtatat attccatcat
      241 ttctttatct cggttactta tttgtttgct gattctgacg gccaatcgtt cttcaagttc
      301 ttttaactgt ttgttcatgt gttcctgttc tgatcgcctt ctgttctcag actcctcaaa
      361 agcagcttta taatgatcta acatttctat tagcttattg ttaacagatt gttgctgttc
      421 aaattgattt ttcattgtat ttaataccgc atctgttctg tgattgttat gttcttgagt
      481 caaacttaac tcacgattat tttcttcttt ttttaagtgt tcctcttgtg tgcctgatga
      541 ggaacggttt ctatcaaatc cctttataac agtcattgca gctatctcaa gagataactt
      601 tttttcttga atagcatatt ttaggtttga taataatgac tggtcagtaa cagaatagta
      661 acgctttttt ctgttatctt taataaactc atacccatgc ttttctagtt caatacacca
      721 ttttctaata ttactggaag ttgtatttaa tcgcttggcc atttcttcag catttattgt
      781 atcctgcttc tcattcatac ttggcctccg ttcctaatgt gttatcgttg tgatcctttt
      841 atgatcttta tataatacaa ttttatttaa ataaattcga gagacctttg ttatatcctt
      901 tagcttagta aaatttaaag agtggtttta tataataatt caacttggcc gtaaaaaaat
      961 aaaagagcaa cctctgcagt ggacgggagg ttgctcaaaa atagggttac gttgaaattt
     1021 gtaaaaaata gtctgacttt taatatatca tatttgtata caaaatcaat tatttgaact
     1081 gttctgcata tgaataaaag gtgcgctttt tattcatcat ctcttcattt tcctgtaagg
     1141 agaactccgt catatcaacg atgtataaaa agctgcatac tccggaaaaa gaaccctcgg
     1201 cggaaccgtt ctgcgacaag ggttcttttt tgggttgtgt tccggaaaga gtggttatgt
     1261 aaactagatc tgtatattat atacaggttg aaaacagaaa aactccccca atgcgttaag
     1321 gaagttttct gtgtaaaatt cgaataagaa agtccgtaga tgcctcacgg cgtcttaggt
     1381 agtttgcttc gttatcagga atgtatgtat agaaagagga aaaatctact tctttcatcc
     1441 catagtaatg gggtatcctg ttatcgaaga gtagggggct aattataaga tgtgaaatag
     1501 tgaaaagaac attatacaag ctataaagaa atatcataat tgttggaggt gaaattatgg
     1561 cagttgttaa agctacaact tcagatatcg ctcttctagc aagattactt agagcagaag
     1621 gtgaaggtga gggtgtacaa gggatgcttc ttataggaaa cgtaggaatt aatcgaatca
     1681 gagcaaattg ctctgatttt aaaggactga gaaccattcc acaaatgatt tatcaacccc
     1741 atgcatttga agctactcaa aaaggctatt tctatcaaag agcacgagaa agtgaaaaac
     1801 gtttggcacg tcgatctgtc agaggagaac gtagttggcc tgcaaaatat agcttatggt
     1861 atttcagacc tcctcaaaag tgcccttcaa catggtacaa acaacctctt gttgctcgat
     1921 ataagcttca ttgcttttat caaccaaaag ccgaaacatg cgaaaacatt tataatactt
     1981 tttaaaaatt ccctataatt ttataaagtg ctgtattatt ctatatcatt aaaaataaaa
     2041 aactaacttt gagtgagaaa aaagttagtt ttttattttt aagttgttta gggtatataa
     2101 gtttatattt tattcggccc ttatataatt gattacccca ataattattg gtaagtctcc
     2161 cctttaaaac actctcattt tttaagggat ttcctcaacg ttcaagtcca ctatggagta
     2221 tatttgatgt tagaaaggcc ccaacgggag gcaagtcact gttggtaacg gcaggtccta
     2281 tgagagttgt atctgtcgta gcaatccaag ttccattcct acctgctaat aaagaaggtg
     2341 gtttcataat aactcgccca gttaaaataa gatgtcgaat tctaatagtc gaatcatcaa
     2401 acatagttaa gttaccagaa tatgtggcat aaggtgaatc aaagctgatt tgtttggcac
     2461 tctcatccca atatccatta atgggaataa gagtgccacg aaaattaatt gtacccgtta
     2521 ctctgttacc tgtaatggag ttaataataa gcacacccca aaccatcggt ctacctgcta
     2581 tctcagcatg gataccccat cgtgatggaa atggtatatt aaccctgact ttctctgtag
     2641 gttgcaaagc tgattcctcc ccttttctag ggccataaat tatgaatacc taatacaata
     2701 tgagaaatag gtccaaaatg ttcgaaccct tatgaatata gcaaaaaacc ttcctgttca
     2761 aacgaacatt tctatgttaa ataagttcca ggagaaatat aaaaatgagt cagttaaaca
     2821 aaatacgtta aagagtgttt aagttcagta gtaatgacat cttattagat aagatgtcat
     2881 tatttatttc ctataattgt gattatgtta actacctttg tatatcatat acagggtgaa
     2941 aacagaaaaa actccacagc cggttgggag ttttttctac atacatttgt tgttaggacg
     3001 atgttatctg acatcatgag tagtttgctc aagttcgtaa gaacttatgt ctaatcagta
     3061 gttttttctt catgagatat agcaacttca tctttataat caaaatctgc tttttgactg
     3121 attttccctt cttcaaagat tacttggaat ctaatcttct taatattgtc ccaagatcct
     3181 ctaccaaata gaaatggatt catttcataa gttccagttc cttttctaat cataatctct
     3241 tctttaacaa gcttattgat gttgttattt aaactttgaa ctgtattaaa ccccaattct
     3301 tcagccattt gtttttttat gtatgcattt aaaacaacga tgttatcata ggccattctt
     3361 tttagaagaa catttaatgt cttctgaata ccacttgata ggttatttat ctgtaagata
     3421 ttatctaaat aaatttttac gtaatcaggt tctttggaaa cgtatgattc ttcgatatgt
     3481 ctttgaaata gcttctacag cgatgttata aacatctgta ataaacgcgt attgatgatg
     3541 taagatatct tttaattctt gatcttgaat atgttgatca aaaagttgat attgatccag
     3601 cacattaata aaaccagcta gaatttcatg catgtcaaac atttcatggc cgccatgatt
     3661 catctgcggt gccatattag gcgattgttg tgtattagat tgattcattc cattttgcat
     3721 tctctttacc tccagatttt taacattctt ttttaaactg tacaaatagt aaagagaata
     3781 tgtaggatat gtgattatat aaaccttaaa gcgctaaaac aacttaaagt tggttctaat
     3841 tgccttaagg caatttttaa tgattaacgg ctaagatctg gaccttgact tttatttcta
     3901 tttatagagc tacttctgaa ccttctaagg tgctgtcttt gttgagcctg ctcttcactg
     3961 cgagtagctt gttgaatcgc attaaagatg cctgagactg ttcctatgat gttttcttta
     4021 gtttttattt gcttttctat agttggtatt acgttttgct caagtcctct aatttcgctt
     4081 tgtcgcttgt ttaaatcatc tagtccttta tatccttttt ccttaaggtt ctccctgtag
     4141 tgatttactt cgtgttgtaa ttcttcatag tgttgacgtg ttttctttga aactaagcgt
     4201 tttgctgctt caccagggtt attatttaat ttatcaagtt cattttttga tttttcaagg
     4261 ttagtaactt ggctcttcat tttatctaga tgatcttttt cgatttgaaa gatttttaga
     4321 tctttcctta gtttatataa gtcatctttt tgatttttaa ttgctttttt aatgacctct
     4381 aacgttactg gagtcttttt ggccatatct atatgttttt ctagttttaa tgcattttca
     4441 taacttaaat agtttccagc tgttttcaag tctggatagt gagagcttac ttcacgaatt
     4501 ttagccgttt taatggcttc ttctgcttgt gacaaagttt ttgtctctac tcggatctgt
     4561 tgcttttcat acttaatttg atgcatgcgg ttctcttttt gttgcgagaa ctcttgatag
     4621 cgttggtaga attcttcttc atttgaaaaa cgaagggatt ctttatattc ttcgatgctg
     4681 ctttcatagt ttgctttatt actgtctaaa tcagatattt tatcaaggta acgacgtttc
     4741 atcatattat tttctttcag cttaaacgga ttaagtgatc ccttgagttc atctactttt
     4801 tcttcattct gcttaatttc acgctctaag gctctcagac ggtcatagta attgttgacg
     4861 ttcaagaatt gctgttgttg gacatgaaaa tggcgttcaa tcggatcatg acgatccgcc
     4921 attttacgta gttctttctg gcgtcttgaa atatcgtcta aggaaatgac tttttgatct
     4981 aaaagagatg cagctttttg gatataggtt ttttcaacat ctgtagagaa gctaaattgc
     5041 ttcttttttt ctttcatttt ttggacatga gcttcttttt gacggcgata agcttgtaaa
     5101 tcaataacaa cttgattgta ttcttggcgc tctcggttga cttgaccgcg ttcgctttgt
     5161 ttccctttct tctccatgtc atgtgcgaca tggcctaagt gaaccatagg caaggtttcg
     5221 agtccacgct tttcatgaga aagatgagtg atacgctcat tgattttagc ttgttccaag
     5281 gctttgttgg catagcttgc ccactgttca cgaatggata agcagccttc gctgtttttg
     5341 acgtacccac ggttgttctc ttttacattg gcaaaatcag cattccagtc acgattcttt
     5401 ttgctaaacc catcttgatt cagatttctc atcgtcaaca tgatgtgagc atgcggatta
     5461 ttggtatcat cgcgatggat agcaatatca gcaaccattc ctttttgaac aaactcgttt
     5521 tgagcaaaag atcgaatgag ttctttttgt tggtcgtgat tcaattcaat tggcaacgca
     5581 atgtttaatt cacgtgccaa ttgtgagtct ttgcgtttct ctactttttc aacttcattc
     5641 cataaacggt tccgatcttt catccattct ggtgcattgg taggagtcag aatcatggtt
     5701 tccggctgtg cgtgacgagc ataaaatttt tgctcgtctg tacgttcatc atgtagtttt
     5761 tcgccactgc gataagcagc tgcagcaaca gccgattgtc cttttgaacg actgatgatt
     5821 tgcatcgata gatggtagat agccataatt ctttcctcct ttcttgagaa tgggttagtg
     5881 gattaggaat aagttgatga gagcacaatg tgcgctaatc aatgatttgg atgaaatcag
     5941 catcatgaac accaaaggtg tcagatggtg gtttcatctt tgccgatagg caaacagatt
     6001 ttataaaatt cgccatctag gcgaatggat ctttggtttt gtcttttcgt tccgaaggaa
     6061 ccgcacacat cggtacgatg tataagtgcg cccttatctc ttcggtctaa gggaattata
     6121 ccatatcctg gtgagagaat ctatgctaag gataccaatt tatctcaatt tttgatataa
     6181 tcgatttaaa cattagagaa cagaaaaagg agttgaggat gggtgacgaa aaagcgaaca
     6241 gatgaggaaa ttcttcagga gttagaagag aaaattgaaa agatgaaagc gaagaaacaa
     6301 caggttgaag cgcgtaaaaa ggaaaaagag agaaaagaaa gaacaagacg actaattcaa
     6361 gtcggcgcga tctttgagaa atactttgag attcgaagtg aagaagaagc agaaaaaatt
     6421 gcgaaggcat tacaatctta tgtaggtaaa aacaaagata agattttaca tcatgatgta
     6481 gttgtaaaag aaaaaacgaa agcagcagct gaagtagctg tttctgaaga ataaggatcc
     6541 gtaggagcct attatgaaat gtttatattg tgattcagat aataattcta tggacaaagg
     6601 atgttctgtt tgtggagcac ctttaaacat aaagcgtcct caacttaaag aatatgtgag
     6661 tttacaagat tcagctttgt cattccagga gcttcaaagt tttcacacat atgatttgtt
     6721 aatcttgctc cggttggttc gagaagagcg ttcaaaaagc tataatttga tgagaacggt
     6781 tcagaaagcg ccagaagggg ctgagattga taaaagtgta atagagttcg gagaaagcga
     6841 atatcgcctt tatacggcac gtatgaagct tatagagggt cttttgatag atcgtatggg
     6901 gtacaagcct aaaagagtag atgataagct gcttgcagcg ttaaaacatc gaatggggtc
     6961 gaaataggct tgcattcatg aaagatttgg tagaaaatta gacataagaa aaagccctca
     7021 agttttggtc gactcggagg acttgttctt aaacaaatat taaggaaggg gttaaccttc
     7081 tgttgtacat attattataa aggcattata acataagttg gttaattcct tccacaaaaa
     7141 ccgggggaat tataaaaatg aaaaaagtaa gtgatttaca aaattcttta aatgaagctg
     7201 agcaaaaagc cagactaaga gactttgaaa aagatcaaaa ctcctttcaa aaagcagaag
     7261 atctaaaaaa atcattatta gagcaattaa aagaaattac aggtgaagac cattttgttg
     7321 gaactagtaa agatccattt gcaggggagc cgtttaatca gactatgcat aggaatttag
     7381 atttattaaa tcaaattgat tatttaacac aagctgaagg ggatttctta tttagaattc
     7441 aaccatattt agaatttaaa agtaatgcaa ttattcgaag agaagataag tttaaaaaga
     7501 ataagaagag cactgtagat cttgaagatg attatgaact tgttcagtcc gcaacagttt
     7561 ctgatattgc tgaaatgatt ggtaaaacaa gagcaaatac atcaaaaata atgcaatctt
     7621 taaaagaaaa agagattttg cttaatccag aaggagcagg acaagtaatc gacaatggac
     7681 gtactgttag tccgcgaaca tggattgtaa atccttatat tatgatctgt gcgccacgta
     7741 aaaacaaagt tcaattagat agattaacga tgcgtttatt tcaaaactct ttgaaaaacc
     7801 ttaaagatca aaatggtaaa aaggtaaaat taccagctag attcttttaa tactattttt
     7861 ctttaaaaag cactttatat tttagagtgc tttttgtttt tgttaaatat taggtgtatc
     7921 gaaaacgata cattttagtt tgtttttgta tcgaaaacga tacaccccta aaactgcctt
     7981 cagaccagtc atatcaaggg tttgggacgt ttttttgttt tatcccctat tatcactatt
     8041 atttaatagc aa
//
