LOCUS       NZ_CP069407             9349 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia megaterium strain CDC 2008724129 plasmid pPmC-129-10,
            complete sequence.
ACCESSION   NZ_CP069407
VERSION     NZ_CP069407.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16083678
            Assembly: GCF_017086545.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Shwed,P.S., Crosthwait,J., Weedmark,K., Hoover,E. and Dussault,F.
  TITLE     Complete Genome Sequences of Priestia megaterium Type and Clinical
            Strains Feature Complex Plasmid Arrays
  JOURNAL   Microbiol Resour Announc 10 (27), e0040321 (2021)
   PUBMED   34236233
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   Shwed,P., Crosthwait,J., Weedmark,K. and Dussault,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Biotechnology Lab, PS-Biotech Lab, 251 Sir
            Frederick Banting, Ottawa K1A0K9, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017086545.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 06:19:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,820
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 199
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 148
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 24 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 25 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069407.1.
            Bacteria and source DNA available from CDC; Dr. C. Beesley.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9349
                     /organism="Priestia megaterium"
                     /mol_type="genomic DNA"
                     /strain="CDC 2008724129"
                     /host="Homo sapiens"
                     /db_xref="taxon:1404"
                     /plasmid="pPmC-129-10"
                     /geo_loc_name="USA: Rhode Island"
                     /collection_date="2005/2006"
     gene            complement(join(9330..9349,1..619))
                     /locus_tag="ICR95_RS28625"
                     /old_locus_tag="ICR95_28625"
     CDS             complement(join(9330..9349,1..619))
                     /locus_tag="ICR95_RS28625"
                     /old_locus_tag="ICR95_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002192154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_234943163.1"
                     /translation="MKKALEQAEKKARIRDSKIEKIIGQAEVGLSAEQQKMLLQILHKT
                     TGEDYFIGKRKKKTDGVKFVQMITENIDYLCEIGYLTQAEKAFLFEISRFLEFKSNVIV
                     EKNTGDEGKPAAASPSYLAKKLNKTRTSISKMMNELLDKGVLGVAETGVTTEDGRSCSS
                     RTWFVNPNILCNAPKDEIDRATQQIFSKALRNIKVGEVKKKHKLPIYLF"
                     /name="MarR family transcriptional regulator"
     gene            complement(803..1219)
                     /locus_tag="ICR95_RS28630"
                     /old_locus_tag="ICR95_28630"
     CDS             complement(803..1219)
                     /locus_tag="ICR95_RS28630"
                     /old_locus_tag="ICR95_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014462149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_078990663.1"
                     /translation="MMKCLYCDSENNSIDQACSVCGAPLYTKRPQLKEYVSLEDSALSF
                     QELQRFHTYDLLVLLRLVREERSKSYNLMRTVQKAPEGVEIDKSVIEFGESEYRLYTAR
                     MKLIEGLLVDRMGYKPKRVDDKLLAALQHRMESK"
                     /name="hypothetical protein"
     gene            complement(1236..1547)
                     /locus_tag="ICR95_RS28635"
                     /old_locus_tag="ICR95_28635"
     CDS             complement(1236..1547)
                     /locus_tag="ICR95_RS28635"
                     /old_locus_tag="ICR95_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_062954797.1"
                     /translation="MPKKRTDEEILQELEEKIEKMKAKKQQVESRKKEKERKERTRRLI
                     QVGAIFEKYFEIQSEEEAEKIAKALQAYVGKNKEKILNHDMVVKEKKKAAAEVAVSEE"
                     /name="hypothetical protein"
     gene            1924..3906
                     /gene="mobQ"
                     /locus_tag="ICR95_RS28640"
                     /old_locus_tag="ICR95_28640"
     CDS             1924..3906
                     /gene="mobQ"
                     /locus_tag="ICR95_RS28640"
                     /old_locus_tag="ICR95_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_205664572.1"
                     /translation="MAIYHLSMQIITRSKGQSAVAAAAYRSGEKLHDERTDEQKFYARD
                     IQPETMILTPSNAPEWMKDRNRLWNEVEKVEKRKDSQLARELNIALPIELNHDQQKELI
                     QSFAQNEFVQKGMVADIAIHRDDANNPHAHIMLTMRNIDENGFGKKNRDWNADFANAKE
                     NNRGYVKNSEGCLSIREQWANYANQALEQAKSKERITHLSHENRGLEVLPTIHLGHVAH
                     DMEKKGKKSDRGQINRERQEYNKVVIDLQTYRLQKEAHVQKIKEKEKQFSFSTDIEKTH
                     IQKAASLLNQKIVSLDDISKRQAELRKMSDRHDPIERHFHVQQQQFLNVSNHYDRIKDL
                     NYEIQQNEEKVEELKKSLNPFKLKENNMMKRRHLDKISDLESNKQTYVNGIERYKKSLR
                     FSNEEGFYQRYTEFSQQKENRLHQIKYEKQQIQVETKALLQAEEAIKTAKIREISSHYP
                     DLKTAGKYLSYDNALKLEKHVDMAKKTPVTLEVIEKAIKNQQDDLYKLKKDLKIFQTEK
                     DHLDKIKSQVTSLEKTEKELDTLSNSPGEATKRLVSKKSRQHYEELQHEANHYRDNLKE
                     KGYKGLDDLNKRQGEIRELEQSVIPTIEKDIKVKEHVIETVSGIFDAIQQATRSQEQAQ
                     QRHQLRRMRSTNINRAKDQGPDFSR"
                     /name="mobQ"
     gene            complement(4112..5542)
                     /locus_tag="ICR95_RS28645"
                     /old_locus_tag="ICR95_28645"
     CDS             complement(4112..5542)
                     /locus_tag="ICR95_RS28645"
                     /old_locus_tag="ICR95_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053939.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="WP_195862711.1"
                     /translation="MDVQFSEELHLFAQELQRSLSPIALQEIAKKVGFVKRSSKYQANE
                     LMALCVWLSQEVARTSLAQLCSRLEASTGVLMSQEGLNQRFNPAAVQFLREVFALLLTQ
                     KLCATQSLSSTLFLIFNRIRILDATVFQLPDAFVSDYQGSGGSSNTAGVKIQLEYDLLS
                     GQFLHVQLGPGKDNDKTYGTTCLESVKAGDFCLRDLGYFDLRDLQTIHELGAFYLSRLK
                     LNTRIYVKNPEPEYFKNGTLKKQSEYIQLNMAHILNELEPGETIELSEAYVGQIQKLPT
                     RVIIHRLTNEQTKTRLNNQAVREKKKGIVMKDKSKQLMGMNVYITNTPPKEVPTNDVHS
                     LYSLRWQIEILFKTWKSFFEIDECKTIKKERLECHLYGQLIGILLCSSTMFQMRQLLLK
                     KRKQELSEYKSIYMIKDYFPLLFPAMAGDLTELVTILQRLYHLLKKNGRKCHRYQKKTV
                     FDILGIVYETAVKQQQVA"
                     /name="IS4 family transposase"
     gene            complement(6202..7116)
                     /locus_tag="ICR95_RS28650"
                     /old_locus_tag="ICR95_28650"
     CDS             complement(6202..7116)
                     /locus_tag="ICR95_RS28650"
                     /old_locus_tag="ICR95_28650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_205664571.1"
                     /translation="MKTELSSPHQLTTKVSRKELFIPWVFVLMWSSGAILVELGLPYAD
                     PFTFLAYRFFIAAIIMWIISLKLKSKLPSTTFEWKAIISTGIFQQVGYQAFFFLALDFN
                     ISPGLLAIILGAQPIVTTVLSKGDTTKKQWIGLFSGMIGLILVVGHSLWEGSISILGVV
                     WCLLSLGSITCGTFLQKNIKTSLPMNMSIQYTCSFLLFIIIAPFFGSFTVDWTSTFLMS
                     LLWMVFIITVGATMLLYYMISKGNLTNVTSLFYCVPALTAILDYFIFGNTLGFTPIIGL
                     GFIIMGLVMINQKNKEFKKTVAS"
                     /name="DMT family transporter"
     gene            complement(7306..7437)
                     /locus_tag="ICR95_RS29385"
     CDS             complement(7306..7437)
                     /locus_tag="ICR95_RS29385"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_268834045.1"
                     /translation="MPSYIFGKIDINDNLLKKDLEIHDAFPKIPEEYDEFSSGFWMN"
                     /name="hypothetical protein"
     gene            complement(8035..8589)
                     /locus_tag="ICR95_RS28655"
                     /old_locus_tag="ICR95_28655"
     CDS             complement(8035..8589)
                     /locus_tag="ICR95_RS28655"
                     /old_locus_tag="ICR95_28655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_078990665.1"
                     /translation="MDKYDKAYTPKEINSILEVSESTGRKWCLALEKKGYMFIRNDRNQ
                     RLFTKHDLDALQKFQALLKETNMPLDNASDIIVSMFGKGSFEERTVSVREEIKDKRRSL
                     ERSDETIDTLIKHIQKQEEYMERQELFNQNLLKRLDEQEKYILSRLEDRDQALTASLRE
                     LHQTQKAIAASQEKKWWEFWK"
                     /name="DUF3967 domain-containing protein"
     Typing          complement(2..619)
                     /name="rep_cluster_1852"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.838"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1925..3903
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.939"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcttatgttt ctttttaact tctccaacct ttatatttct aagagctttc gaaaatattt
       61 gttgtgtagc tctatcaatt tcatctttag gagcattaca taaaatatta ggattaacaa
      121 accatgttcg actactacaa gatcttccat cctctgttgt tacacccgtt tctgctactc
      181 ctaatactcc tttatctaat aattcattca tcattttaga tattgaagtt ctagttttat
      241 taagtttttt tgctaaataa ctaggtgaag ctgctgctgg ctttccctca tctccggtat
      301 tcttctcaac tattacattc gacttgaatt caagaaatct agagatttca aacaaaaatg
      361 ctttttcagc ctgagttaaa tatccaattt cacatagata atcaatattc tcagtaatca
      421 tctgcacaaa tttcacccca tcagtttttt tcttacgttt accaatgaaa taatcttctc
      481 cagttgtttt atgtaagatt tgtaaaagca ttttttgttg ttctgccgat aaaccaactt
      541 ctgcctgtcc aattattttt tcaatctttg aatctctaat tcttgctttc ttttctgctt
      601 gttcaagagc ctttttcaat ttaattcctc cgattttgta gaaggaatta accagcatgt
      661 gttataatat aactatcaca atataaataa cggaaggtta accccttctt gtatatttgc
      721 ttagaaaagt cctcctgtcg tccaaaactt gagggctttt tcttatgtct aattttctac
      781 caaatctttc ataaatgcaa gcctatttcg actccattcg atgttgcaac gctgcaagca
      841 gcttatcatc tacccgttta ggcttatatc ccatccgatc tactaaaaga ccttctatga
      901 gcttcatacg ggctgtataa aggcgatatt cgctttctcc gaactctatg acactcttat
      961 cgatctcaac tccttctggc gccttctgga ccgttctcat caaattatag cttttcgaac
     1021 gctcttctcg aactaaacga agcaatacta acaagtcata cgtatgaaaa cgttgaagct
     1081 cttggaatga caaagccgaa tcttctaaac tcacatattc tttaagttga ggacgctttg
     1141 tatataaagg tgctccacaa actgaacatg cttgatctat agaattattt tctgaatcac
     1201 aatataaaca tttcatcata ggcttctacg aatccttact cttcagaaac agccacttca
     1261 gctgctgctt tctttttttc ttttacaacc atatcatgat tcaaaatctt ctctttattt
     1321 ttccctacat acgcttgcaa tgccttcgca atcttttcag cttcttcttc actttgaatc
     1381 tcaaagtatt tctcgaagat cgcgccgact tgaataagtc gtcttgttct ctcttttctc
     1441 tctttttctt ttttacgcga ttcaacttgt tgtttctttg ctttcatctt ctcaattttc
     1501 tcttctaact cctgaagaat ttcctcatct gttcgcttct tcggcatcca ttctcatctc
     1561 ctttttctgt tccctaatgt ttgtatcgat tatatcaaaa attgagataa attggtatcc
     1621 ttagcataga ttctctcacc aggatatggt ataattccct tagatcgaag agataagggc
     1681 gcacttatac atcgtaccga tgtgtgcggt tccttcggaa caaaaagaca aaaccaaaga
     1741 cccattcgcc tagatggcga atttcataaa acctgtttgc ctatcggcaa agatgaaacc
     1801 accatctgac acctttggtg ttcatgatgc tgatttcatc taaagcattg attagcgcac
     1861 cttgtgctct catcaactta ttccaaaaac acaaacccat tctcaagaaa ggaggaaaga
     1921 atcatggcaa tttatcatct atcgatgcaa atcatcaccc gttcaaaagg ccaatcggct
     1981 gttgctgcag ctgcttatcg cagtggtgaa aaactacatg atgaacgtac agacgagcaa
     2041 aaattttatg ctcgtgatat acaaccggaa accatgattc tgactccttc taatgcgcca
     2101 gaatggatga aagatcgaaa ccgtttatgg aatgaagttg aaaaagttga gaagcgcaaa
     2161 gactcacaat tagcacgcga attaaacatt gcgttaccaa ttgaattaaa tcatgaccaa
     2221 caaaaagaac tcattcaatc ctttgctcaa aacgagtttg ttcaaaaagg aatggtcgcc
     2281 gatattgcca ttcatcgtga cgatgctaat aatcctcatg ctcatatcat gttaacgatg
     2341 agaaacattg atgaaaatgg tttcggcaaa aagaatcgtg actggaatgc tgattttgcc
     2401 aatgcaaaag aaaacaatcg ggggtacgta aaaaatagcg aaggctgctt atccattcgt
     2461 gaacagtggg caaactatgc caatcaagca ttagaacaag ccaaaagtaa agagcgtatt
     2521 acgcaccttt ctcatgaaaa tcggggactt gaagtacttc caaccattca cttaggccat
     2581 gtcgctcatg acatggagaa gaaaggaaag aaaagtgacc gtggtcaaat caacagagaa
     2641 cgccaagaat acaataaagt cgttattgac ctgcagactt accgtcttca aaaagaagct
     2701 catgtacaaa aaataaaaga aaaagagaag caatttagct tctctacgga tattgagaaa
     2761 acacatattc aaaaggctgc atctcttttg aatcaaaaaa tcgtttcctt agacgatatc
     2821 tcaaaacgcc aggcagaact acgcaaaatg agtgatcgtc atgacccaat tgaacgtcat
     2881 ttccatgtgc agcaacaaca gttcctaaat gtcagcaatc actatgaccg tataaaggat
     2941 ttaaactatg aaatccaaca aaatgaagaa aaagtagaag aacttaaaaa gtctcttaat
     3001 ccgtttaaac tcaaagaaaa taatatgatg aaacgtcgtc atcttgataa gatttctgat
     3061 ttagaaagta ataaacaaac ctatgtaaat ggcattgaaa ggtataaaaa atcccttcgt
     3121 ttctcaaatg aagaagggtt ctaccaacgc tatacagaat tctctcagca aaaagagaat
     3181 cgtctgcatc aaattaagta tgaaaagcaa caaatccaag tagaaacaaa agctctatta
     3241 caagcagagg aagccattaa aacggctaaa attcgtgaaa taagctctca ctatccagac
     3301 ttgaaaacag ctggaaaata cttgagctat gacaatgcct taaagctaga gaaacatgta
     3361 gatatggcca aaaagactcc cgtaacgtta gaagtcattg aaaaagcgat taaaaatcaa
     3421 caagacgatc tatacaaact aaagaaagac ctaaaaatct ttcaaactga aaaagatcat
     3481 ctagataaga taaagagcca ggttactagc cttgaaaaaa cagaaaagga acttgataca
     3541 ttaagcaaca gccctggtga agcaaccaaa cgcttggttt caaagaaatc acgtcagcac
     3601 tatgaagaat tgcaacatga agcaaatcac tatcgtgata accttaagga aaaaggatat
     3661 aaaggactag atgatttaaa caagcgacaa ggcgaaatta gagagcttga gcaaagtgta
     3721 atcccaacta tagagaaaga cataaaagtc aaagaacacg tcatagagac ggtctcaggc
     3781 atctttgatg ctatacaaca agctactcgt agccaagagc aagcccaaca aagacatcaa
     3841 ttgagaagaa tgagaagtac caatataaat agagcgaaag atcaaggacc agattttagc
     3901 cgttaaagat aaaaaattgc cttaaggcaa ttagaaacaa cttaaagttg ttacatttta
     3961 tttaaagaaa ttaaacctat taggggtagc atcacatacc catcaagcta acgacagaaa
     4021 cgattaaaaa tgtaatgatc cttgtcctaa catcggtgag tatcagaaaa atcggcatga
     4081 caaagaagcc tatgaaacta tcattttttc gttaggcaac ttgttgttgt tttactgctg
     4141 tctcatacac aataccaaga atgtcaaaaa cggttttttt ctgataccga tgacacttac
     4201 gcccattctt tttcaacagg tgatacagtc gttggagaat cgttacgagt tcagtcagat
     4261 cacctgccat tgctggaaat agtaacggaa aatagtcctt aatcatataa attgatttgt
     4321 attcactcag ctcttgtttt ctctttttca acaggagctg ccgcatttga aacatggtgg
     4381 aagaacagag aagaatcccg atgagttgcc catataaatg gcactctagc cgttcctttt
     4441 tgatggtttt acattcatct atttcgaaga aggacttcca tgttttaaat aaaatttcaa
     4501 tctgccaacg taacgaatac aatgaatgca catcgtttgt cggtacttct tttggtggtg
     4561 tgttcgtaat ataaacgttc attcccatca gctgtttact tttatccttc atgacaatgc
     4621 ctttcttttt ctcacgtaca gcttggttgt ttaaacgggt tttagtttgt tcgttcgtta
     4681 gtcggtgaat gatgacacgt gtcggtaatt tttgaatttg accaacataa gcttctgaaa
     4741 gttcaatggt ctcacccggt tccagttcgt tcaagatgtg tgccatatta agttgaatat
     4801 attcgctctg ctttttcagt gtgccgtttt tgaagtattc tggttcaggg ttttttacat
     4861 aaatacgggt atttagcttt aatcgagaga gataaaaggc gcctaattca tgaatcgttt
     4921 gtaaatctcg taaatcaaaa taacctaaat cacgtaaaca gaaatcgcct gctttcacac
     4981 tttcaagaca agttgtgcca tacgttttat cattatcttt tcccggtcct agctgtacgt
     5041 gtaaaaattg cccactcaat aagtcatatt caagctgaat ctttacacct gccgtattac
     5101 tgcttcctcc agacccttga taatcactta cgaaggcatc gggtagctga aacactgtgg
     5161 catctaaaat ccgaatacgg ttaaaaatca agaagagcgt tgacgatagc gattgtgttg
     5221 cacaaagttt ctgtgtgaga agtaaagcaa acacctcacg aagaaactgg acggctgctg
     5281 gattaaaccg ttgattcaat ccttcctgac tcatcaggac acctgttgaa gcttctagtc
     5341 gactacacag ttgtgcaagc gatgtgcgag cgacttcctg gctaagccac acgcaaagag
     5401 ccatcagttc atttgcttga tacttactcg atcgtttcac aaatcccact ttcttagcga
     5461 tttcttgtaa ggcaatggga gacaaagagc gttgtaattc ttgtgcaaat aggtgtaatt
     5521 cttcggaaaa ctgaacatcc ataaaaaaac gccatccttt tcgagtagtt tactaaaaag
     5581 aataacgttt ttttcgtttt ggggaaaatc cattgcgtta gcttgatggg tatggggtag
     5641 catcaggatt aggcggattt acaacattaa gacaaaaaac cttgtatcaa tagggtctag
     5701 cctattggta cataattaaa ttatcgttaa attaggactt cataatttct cataggggtc
     5761 tcaccaacat ttagcggaaa acatacgcta agcagatttc gataagtaaa ttacggcctt
     5821 ttatacgttt agacattaaa ttcgttcaca aacttaatat tttaataatt attgaacatt
     5881 gattattgaa cgagttaatg gacacttccg aagggaagaa acacttataa atcctttaat
     5941 gacaaggtgt tcagcaactc aagattttct gaacaattcc ttagcgtatg ttttcagcat
     6001 ttcgttggtg ggacctctca taagacttgt atcaaaatgc ttaacgttgt aaatccgcga
     6061 ttttctgacg ctaccccaac ttactttgcg gtctacagca ggaaccctgt cactaagcct
     6121 atatgatata caaacttagt ttacataata atgattatgt aataaacaca tttgtatcta
     6181 ccagaaaaaa acagttacat attaagatgc aactgttttt ttaaattctt tatttttttg
     6241 atttatcata actaggccca tgattataaa gcctagccca attattggtg taaaccctaa
     6301 agtatttcca aatataaaat aatctaaaat tgctgttaac gctggtacac aataaaatag
     6361 gcttgtaaca ttagttaagt tccctttaga aatcatatag taaagcaaca ttgttgcacc
     6421 tacagtaata ataaaaacca tccaaagaag tgacattaaa aaagttgatg tccaatccac
     6481 tgtaaaagaa ccaaaaaaag gtgcaattat tataaataaa agaaaactac aagtatattg
     6541 gattgacata ttcatcggta aactagtttt aatgtttttt tgtaagaagg taccacaagt
     6601 aatacttcct agtgataata aacaccaaac gacaccaagt attgaaatag aaccttccca
     6661 tagactatgt cctacaacta aaattaatcc tatcatgcct gaaaataaac ctatccattg
     6721 cttttttgtt gtatctcctt ttgataatac tgttgtgaca ataggttggg ctcctaatat
     6781 aattgctagt aaaccaggag aaatattaaa atctaaagct aggaagaaaa aagcttgata
     6841 accaacttgt tgaaaaatac ctgtagatat gattgctttc cattcaaaag ttgtagaagg
     6901 tagttttgac ttcagcttta aagaaatgat ccacataatt attgcagcta taaaaaatct
     6961 gtaggccaga aaagtaaaag gatctgcata aggaagacca agttctacaa ggatagcgcc
     7021 actactccac attaatacaa aaacccaagg tatgaagagt tctttccttg aaacttttgt
     7081 tgtaagttga tgaggactac ttaattctgt tttcataata cacctcttca taaagatgag
     7141 cttctcatag tattactaat tgagaagctc accaagtttc tagtatatga atgttttact
     7201 taattactgg tacttgtagt tctgtccagt cagaaaagga aaaacgttct cctaattcac
     7261 gtttttctat taaatattga tggacttttt cagccttatc tgccatcaat tcatccagaa
     7321 acctgaacta aattcatcat attcttctgg aatctttgga aaagcatcat gaatttctaa
     7381 atcttttttt aataaattat cattgatatc aatctttcca aaaatgtaag acggcattcc
     7441 ttcgttcctc tccattcacc tttgtatttt gccctatgac ttttaatctt aagactttct
     7501 tattatataa aaaagaaaag taataatcta ctataaaatt acaaattata taaattttta
     7561 gaaaatatta ttattttttg taatttaatt gaaaatagtg acatttatac tatggttcaa
     7621 atgaatttct actggatttt ataataaaag gattatgata aaatttttaa tttctatgtt
     7681 ttccttttca gatgtatatt atatacaaaa ctagtttaca taacacctct ttccggaacc
     7741 catcacaaaa aagaaccctt gatagacaag ggttcacaga aagaagagtt ttatcgatta
     7801 tgcagctttt tatacatcct tgatttatca acgatgattt gaggaatcat aagcaggaag
     7861 atgcataaaa agctcacctt ttattcatat gcttcagacc caaaaaatag catttgatac
     7921 cataatatgc tatatttaag accagacgat ttttacaaat ttcaacgtag caaaaaagca
     7981 acctccatct cctatgacaa agatgaggtt gctcttttta tttttaatcc ttatttattt
     8041 ccaaaactcc caccactttt tttcttgaga agctgctata gctttctggg tttgatgtag
     8101 ttctctaaga gaagcagtaa gtgcttgatc cctatcttca agacgagaca atatatactt
     8161 ttcttgttca tctaaacgct tcaacaagtt ttggttgaac aattcttgtc tctccatata
     8221 ttcttcttgt ttttgaatgt gcttaataag agtatctata gtttcatcag aacgctctaa
     8281 agaacggcgt ttgtctttaa tttcttctcg aacggatacc gttcgctcct cgaacgatcc
     8341 tttaccaaac attgatacta taatatctga ggcgttatcc aatggcatat ttgtttcttt
     8401 taataatgct tgaaattttt gcaaagcatc gagatcatgt ttagtaaata aacgctggtt
     8461 tctatcattt ctaataaaca tataaccttt tttctctaaa gctagacacc actttcttcc
     8521 cgtactttct gacacttcta atattgaatt tatctcttta ggagtataag ccttatcata
     8581 tttatccatg cactaccact ccaattcttt cattttactg ttcttctcac gttcgtcaaa
     8641 atagattaat agttattagt attacatctt ctaagtaaat aaaatatatc cttcagaaca
     8701 tcaaattctt tatttactaa ggatttattt ctatttactt aagcgttctc caaagtaacg
     8761 cctaacgtta tagcactaag aacgccgttc ttcctaatca taaaatactt tcctggttaa
     8821 ctacaacata aaaagggcga ccattattgt tatgattaat acatacataa ataaaaatta
     8881 tttaaaagga tgtggccgtc ataaagatat gtcggatttg caacgatgaa attgaagaca
     8941 ttgataaagt aatgttaaga agaattagaa aagatgctat gaagtttcca agatcaaaat
     9001 taaaagaaat ggcaagatta acaactatta tgagaataaa agctatagaa aataaaaatt
     9061 cttaggcctc tctaggcctc cttaaggagg ccataaaagc cttaaggctt tatatttttc
     9121 ctaacgaaaa aattataaaa agacccttgc aattctatga gcgaattgct atttatatag
     9181 agtgagaaag gataagtaca agaatgttgt taaatcaacg tttttgaagg gtgaggtgtt
     9241 aaaaaatttt aacacctgtg ttaaaatttt ttaacaccta tttttttaca attcataaga
     9301 acaaaaaaac tgcaagaaga atctcgcagt taaaatagat aaatcggaa
//
