LOCUS       NZ_CP069406            12225 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia megaterium strain CDC 2008724129 plasmid pPmC-129-9,
            complete sequence.
ACCESSION   NZ_CP069406
VERSION     NZ_CP069406.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16083678
            Assembly: GCF_017086545.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Shwed,P.S., Crosthwait,J., Weedmark,K., Hoover,E. and Dussault,F.
  TITLE     Complete Genome Sequences of Priestia megaterium Type and Clinical
            Strains Feature Complex Plasmid Arrays
  JOURNAL   Microbiol Resour Announc 10 (27), e0040321 (2021)
   PUBMED   34236233
REFERENCE   2  (bases 1 to 12225)
  AUTHORS   Shwed,P., Crosthwait,J., Weedmark,K. and Dussault,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Biotechnology Lab, PS-Biotech Lab, 251 Sir
            Frederick Banting, Ottawa K1A0K9, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2020
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017086545.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 06:19:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,820
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 199
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 148
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 24 of 75
            Pseudo Genes (incomplete)         :: 36 of 75
            Pseudo Genes (internal stop)      :: 25 of 75
            Pseudo Genes (multiple problems)  :: 10 of 75
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069406.1.
            Bacteria and source DNA available from CDC; Dr. C. Beesley.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Priestia megaterium"
                     /mol_type="genomic DNA"
                     /strain="CDC 2008724129"
                     /host="Homo sapiens"
                     /db_xref="taxon:1404"
                     /plasmid="pPmC-129-9"
                     /geo_loc_name="USA: Rhode Island"
                     /collection_date="2005/2006"
     gene            complement(join(11706..12225,1..938))
                     /locus_tag="ICR95_RS28560"
                     /old_locus_tag="ICR95_28560"
     CDS             complement(join(11706..12225,1..938))
                     /locus_tag="ICR95_RS28560"
                     /old_locus_tag="ICR95_28560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="WP_205663994.1"
                     /translation="MPIINRFSILACGAMTFTGNTLGLSQLLNQNQAGVQGSIGAFTTL
                     NNALQVPTFPVGTTLNINQNSSAANLVIPNNSNVLYAELIWGGNYLSRNQNIIALINNP
                     VTFVDSNNNSFSINPDPLTQNQETFTAGNPPVTRGFYSRSANVTAIVQNSGAGTYSVAG
                     VPGLLDPLQASTADTNHAGWTLAVIYENSQLPNRSMYLYIGTNGVVNSPANPIIDVPIV
                     GFMTPPSGPVNTRILISAQEGDANITGDQALFGPTALSLTNLSGPRNPSNNFFGSQINN
                     DSGNLDTTGTFGNRNQNPIIATNISAGRQGWDITNVDATNYLGNNEMIATFRYTSTGDA
                     YMPNALGVQIDQGDAVLDVQKRVNTTMADILDTLTYTITITNEGVVPANNVVFTDLIPE
                     GSVFINDSVVINGVSTPGVNPETGISLPDINVDETVTIVFEVQILKCVCFLRNKSFITF
                     SCGKITFSNQVLTVICKSCSGSNCICD"
                     /name="DUF11 domain-containing protein"
     gene            complement(958..1332)
                     /locus_tag="ICR95_RS28565"
                     /old_locus_tag="ICR95_28565"
     CDS             complement(958..1332)
                     /locus_tag="ICR95_RS28565"
                     /old_locus_tag="ICR95_28565"
                     /inference="COORDINATES: protein motif:HMM:NF024556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-Ena type endospore appendage"
                     /protein_id="WP_025908111.1"
                     /translation="MCICKNGYEGILNKCSNNQMFIAKKCVPFRSPCDGLTRTTIYTTS
                     NVVNTSLANGIIAIVNTSIDCVMNVSITDSSGENTYVVSAQSSLVVQVFSLSLITVVCG
                     PDNDNGEDFCTGTFEATLSF"
                     /name="S-Ena type endospore appendage"
     gene            complement(1572..3002)
                     /locus_tag="ICR95_RS28570"
                     /old_locus_tag="ICR95_28570"
     CDS             complement(1572..3002)
                     /locus_tag="ICR95_RS28570"
                     /old_locus_tag="ICR95_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053939.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="WP_195862711.1"
                     /translation="MDVQFSEELHLFAQELQRSLSPIALQEIAKKVGFVKRSSKYQANE
                     LMALCVWLSQEVARTSLAQLCSRLEASTGVLMSQEGLNQRFNPAAVQFLREVFALLLTQ
                     KLCATQSLSSTLFLIFNRIRILDATVFQLPDAFVSDYQGSGGSSNTAGVKIQLEYDLLS
                     GQFLHVQLGPGKDNDKTYGTTCLESVKAGDFCLRDLGYFDLRDLQTIHELGAFYLSRLK
                     LNTRIYVKNPEPEYFKNGTLKKQSEYIQLNMAHILNELEPGETIELSEAYVGQIQKLPT
                     RVIIHRLTNEQTKTRLNNQAVREKKKGIVMKDKSKQLMGMNVYITNTPPKEVPTNDVHS
                     LYSLRWQIEILFKTWKSFFEIDECKTIKKERLECHLYGQLIGILLCSSTMFQMRQLLLK
                     KRKQELSEYKSIYMIKDYFPLLFPAMAGDLTELVTILQRLYHLLKKNGRKCHRYQKKTV
                     FDILGIVYETAVKQQQVA"
                     /name="IS4 family transposase"
     gene            3332..3607
                     /locus_tag="ICR95_RS28575"
                     /old_locus_tag="ICR95_28575"
     CDS             3332..3607
                     /locus_tag="ICR95_RS28575"
                     /old_locus_tag="ICR95_28575"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_062954791.1"
                     /translation="MMDMAVREVERKITKIGNSSGVTFPPEVLEHLNVKQGEMVVFDLE
                     KDGKVVLKKRPAVNVEGFEEIDADFLEGMKDLFDRYDNTLRNLADR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            3638..4033
                     /locus_tag="ICR95_RS28580"
                     /old_locus_tag="ICR95_28580"
     CDS             3638..4033
                     /locus_tag="ICR95_RS28580"
                     /old_locus_tag="ICR95_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664895.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_047933936.1"
                     /translation="MTKYLTHKQAIFLNGVMIQRYTPKEHIGVKDEKLLDSAIMRPQSS
                     AFGSDAYPSLELKAAALFESLAQNHSFHNANKRTAFACLFLFLRTNGWILNVREKEAED
                     MTSFVVTNKPSLQHIADWISSNLVQRS"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(4124..4648)
                     /locus_tag="ICR95_RS28585"
                     /old_locus_tag="ICR95_28585"
     CDS             complement(4124..4648)
                     /locus_tag="ICR95_RS28585"
                     /old_locus_tag="ICR95_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002190312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2695 domain-containing protein"
                     /protein_id="WP_047933934.1"
                     /translation="MDSLLELKDELIKGQKLAMQGSYQRRAPSKKAIPHLLAARKGLKE
                     YVEQHPTDAFAWQLLSQAEEYLLNYKAALSALQNAVTLNKKDRKLVKKLVLLKEQANKW
                     HELDLSPEELGSLEAFLDEKVDIQGCDHTLLYTKEWLDTHISVSKKAKVVKALQNQGGF
                     CDCEVLMNVID"
                     /name="DUF2695 domain-containing protein"
     gene            complement(5137..5733)
                     /locus_tag="ICR95_RS28590"
                     /old_locus_tag="ICR95_28590"
     CDS             complement(5137..5733)
                     /locus_tag="ICR95_RS28590"
                     /old_locus_tag="ICR95_28590"
                     /inference="COORDINATES: protein motif:HMM:NF024551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_205664570.1"
                     /translation="MSEQINEIVYLGGEVAKSLGIARSSLTKYCIALENKDYKFIRGSN
                     NSRAFRSKDILLLQRMKDLVQDKSMTMETAVNVVLSMLPEEERTGTVLEENKVIPQENQ
                     SEEQKENNQLSVLMDKLEYIEEISDRLNSMEKELKDIKTQNKILQDQNEELKTNVETSS
                     NKRDENLLSLIREVQDTKKMIAAAQEKKWWEFWKS"
                     /name="DUF3967 domain-containing protein"
     gene            complement(6226..6843)
                     /locus_tag="ICR95_RS28595"
                     /old_locus_tag="ICR95_28595"
     CDS             complement(6226..6843)
                     /locus_tag="ICR95_RS28595"
                     /old_locus_tag="ICR95_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162395.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_100348231.1"
                     /translation="MAKNIVNFKEAEKNALSRDFDLEKIKYALDHGGVTKEDLEEAMGL
                     LSKATGKDLYVGTKRSPQSKVRFAQSMQNNLGFLYKQDYVTNKEKVFLTDIIPYIAFSS
                     NCIVHDIKEKNPVPANISEIAKLIGITRQNTSLAVNSLVKKGLLFKGDSGVEGNNAKAY
                     AIFVNPHIIYAGDKDNVNEALKVMFHKAMKMKVLKDLPDKLF"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7030..7446)
                     /locus_tag="ICR95_RS28600"
                     /old_locus_tag="ICR95_28600"
     CDS             complement(7030..7446)
                     /locus_tag="ICR95_RS28600"
                     /old_locus_tag="ICR95_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014462149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_205664568.1"
                     /translation="MMKCLYCDSENNSIDQACSVCGAPLYTKRPQLKEYVSLEDSALSF
                     QELQHFHTYDLLILLRLVREERSKSYNLMRTVQKAPEGIEIDKSIIEFGESEYRLYTAR
                     MKLIEGLLVDRMGYKPKRVDDKLLTALKHRMDSK"
                     /name="hypothetical protein"
     gene            complement(7463..7774)
                     /locus_tag="ICR95_RS28605"
                     /old_locus_tag="ICR95_28605"
     CDS             complement(7463..7774)
                     /locus_tag="ICR95_RS28605"
                     /old_locus_tag="ICR95_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_062954797.1"
                     /translation="MPKKRTDEEILQELEEKIEKMKAKKQQVESRKKEKERKERTRRLI
                     QVGAIFEKYFEIQSEEEAEKIAKALQAYVGKNKEKILNHDMVVKEKKKAAAEVAVSEE"
                     /name="hypothetical protein"
     gene            8151..10139
                     /gene="mobQ"
                     /locus_tag="ICR95_RS28610"
                     /old_locus_tag="ICR95_28610"
     CDS             8151..10139
                     /gene="mobQ"
                     /locus_tag="ICR95_RS28610"
                     /old_locus_tag="ICR95_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_205664569.1"
                     /translation="MAIYHLSMQIISRSKGQSAVAAAAYRSGEKLHDERTDEQKFYARN
                     VQPETMILTPSNAPEWMKDRNRLWNEVEKVEKRKDSQLARELNIALPIELNHDQQKELI
                     RSFAQNEFVQKGMVADIAIHRDDANNPHAHIMLTMRNLDQDGFGKKNRDWNADFANAKE
                     NNRGYVKNSEGCLSIREQWANYANQALEQAQSKERITHLSHEKRGLEVLPTIHLGHVAH
                     DMEKKGKQSQRGQINRERQEYNQVVIDLQDYRRQKEAHLQKMKEKEKQFSFSTDVEKTQ
                     IQKAASLLNQKIVSLDDISKRQAELRKMSDRHDPIERHFHVQQQQFLNVSNYYDRVKAL
                     EHDIKQNEEKVDELKGSLNPFKLKENNMMKRRYLDKISDLEGIKANYESSIKEYKESLR
                     FSNEEEFYQRYQEFSQQKENRLHQIKYEKQQIRVETKTLLQAEEAVKMAKIREVSSHYP
                     DLKTAGTYLSYDNALKLEKHIDMAKKTPVTLEIIEKAIGNQKDELYKLKKNVKIFETER
                     DYLSTMKNQVTNLEKTEKELDQLSNSPGETAKRLVSKKARQHYEELQHEANHYRENLKE
                     KGYKGPDDLEKRQGEIRELEKNVIPNIEKQINTKEQVIGTISGIFDAIQQATRSEEQAQ
                     QKQQQQLRRFRSSSINKPKDQGHDFSR"
                     /name="mobQ"
     gene            complement(10274..11146)
                     /locus_tag="ICR95_RS28615"
                     /old_locus_tag="ICR95_28615"
     CDS             complement(10274..11146)
                     /locus_tag="ICR95_RS28615"
                     /old_locus_tag="ICR95_28615"
                     /inference="COORDINATES: protein motif:HMM:NF024556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-Ena type endospore appendage"
                     /protein_id="WP_205663447.1"
                     /translation="MDKYYISNREELAISADKICDWVQKPIELKMRESILEKAPVHDVF
                     CQEFSIAEFKPSLYSLWKTINMESISCTLSLTITECSTNKLLLSVNKGEKDEKQFPIYK
                     GTFNFTFSNISSISIINDTETAYKGRFSIQIHYEIPTTEIDFSCQPICYLDESQVKCKE
                     ISDKTKFKNKVILIHEKWVTLRKINILIEGVFLVEFKKKNGGVFLCEIPFKDIISVYMC
                     VTEKSKIECKLTISDSTCYLAKENIKNCYTLLINNFLCLNIMAIESVVLCIEGFEDINP
                     RDEEIKSIE"
                     /name="S-Ena type endospore appendage"
     gene            11299..11658
                     /locus_tag="ICR95_RS28620"
                     /old_locus_tag="ICR95_28620"
     CDS             11299..11658
                     /locus_tag="ICR95_RS28620"
                     /old_locus_tag="ICR95_28620"
                     /inference="COORDINATES: protein motif:HMM:NF024556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-Ena type endospore appendage"
                     /protein_id="WP_205663993.1"
                     /translation="MSCNSDYNSAILGNCSRENVKDQLCCQFSIAAGADETIYEAINET
                     VIASFILKNSGTFPFDVTATNSAGTTVLTETLAPGQCLMRTIDNLGAISIAGPVAPAGP
                     AKGELDIVVRYQLDS"
                     /name="S-Ena type endospore appendage"
     Typing          8152..10121
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.259"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcccaccctt gacgtccagc agatatatta gtggctatta taggattctg attacggttt
       61 ccaaaagtac ctgtagtatc taaatttcca ctatcgttat taatttgaga tccaaaaaaa
      121 ttattagatg gatttcttgg accagagaga tttgttaaag aaagtgctgt aggaccaaat
      181 aatgcctggt cacctgtaat attagcatca ccttcttgag cacttattaa gattcttgtg
      241 ttaacaggac cagaaggtgg agtcataaag cctacaatag gaacatcaat gataggatta
      301 gcaggactgt ttactacacc attcgttcca atatataagt acattgatct attaggcaat
      361 tgactgtttt cataaattac tgctaaagtc caccctgcat gatttgtatc agctgtagaa
      421 gcctgtaatg gatctaaaag tcctggaacc cctgcgacag aatatgttcc agcaccacta
      481 ttttgaacaa tagcagttac attagcagaa cgactataaa accctcttgt aacaggtgga
      541 tttccagcag taaatgtttc ctgattttgt gtcagagggt ctggatttat actaaaagaa
      601 ttattattcg aatcaacgaa agtaactgga ttgttaatta aagctataat attctgatta
      661 cgagataagt aattaccacc ccagatcagt tcagcgtaaa gtacattact gttgttaggt
      721 atgaccaaat ttgctgcaga actattttga ttaatattaa gagttgttcc aactggaaaa
      781 gtagggactt gaagcgcatt gttaagtgta gtgaatgcac caatgcttcc ttgaactcct
      841 gcttggtttt gatttaataa ctgactaaga cctaaagtat tacctgtaaa agtcatagca
      901 ccacatgcca aaattgaaaa acgattaatt ataggcataa acctgtcttc tcctattcta
      961 aaaagataac gttgcttcga acgttcctgt gcagaagtct tccccattat cattatctgg
     1021 gccacatacc acagtgatta aagaaagtga aaatacttga actactaaag aactctgagc
     1081 agaaacaaca taagtatttt cacctgaact atctgtaata gatacattca tgacacaatc
     1141 tatacttgta ttaacaattg caattattcc attggctaga gaagtattaa caacatttga
     1201 tgtagtgtat atagtagttc tagttaagcc atcacaaggg cttctaaaag gaacacactt
     1261 tttagctata aacatttgat tattactgca cttgtttaat atcccttcat atccgttttt
     1321 acatatacac attttagatt ctccttttct ccttactata aagatatgga gaatagcatt
     1381 tattgtgtga aaaatgggta tttacaaact ataaagatag gaaaatcaat gaatattata
     1441 taggggtctc gccacatacc catcaagcta acgacagaaa cgattaaaaa tgtaatgatc
     1501 cttgtcctaa catcggtgag tatcagaaaa atcggcatga caaagaagcc tatgaaacta
     1561 tcattttttc gttaggcaac ttgttgttgt tttactgctg tctcatacac aataccaaga
     1621 atgtcaaaaa cggttttttt ctgataccga tgacacttac gcccattctt tttcaacagg
     1681 tgatacagtc gttggagaat cgttacgagt tcagtcagat cacctgccat tgctggaaat
     1741 agtaacggaa aatagtcctt aatcatataa attgatttgt attcactcag ctcttgtttt
     1801 ctctttttca acaggagctg ccgcatttga aacatggtgg aagaacagag aagaatcccg
     1861 atgagttgcc catataaatg gcactctagc cgttcctttt tgatggtttt acattcatct
     1921 atttcgaaga aggacttcca tgttttaaat aaaatttcaa tctgccaacg taacgaatac
     1981 aatgaatgca catcgtttgt cggtacttct tttggtggtg tgttcgtaat ataaacgttc
     2041 attcccatca gctgtttact tttatccttc atgacaatgc ctttcttttt ctcacgtaca
     2101 gcttggttgt ttaaacgggt tttagtttgt tcgttcgtta gtcggtgaat gatgacacgt
     2161 gtcggtaatt tttgaatttg accaacataa gcttctgaaa gttcaatggt ctcacccggt
     2221 tccagttcgt tcaagatgtg tgccatatta agttgaatat attcgctctg ctttttcagt
     2281 gtgccgtttt tgaagtattc tggttcaggg ttttttacat aaatacgggt atttagcttt
     2341 aatcgagaga gataaaaggc gcctaattca tgaatcgttt gtaaatctcg taaatcaaaa
     2401 taacctaaat cacgtaaaca gaaatcgcct gctttcacac tttcaagaca agttgtgcca
     2461 tacgttttat cattatcttt tcccggtcct agctgtacgt gtaaaaattg cccactcaat
     2521 aagtcatatt caagctgaat ctttacacct gccgtattac tgcttcctcc agacccttga
     2581 taatcactta cgaaggcatc gggtagctga aacactgtgg catctaaaat ccgaatacgg
     2641 ttaaaaatca agaagagcgt tgacgatagc gattgtgttg cacaaagttt ctgtgtgaga
     2701 agtaaagcaa acacctcacg aagaaactgg acggctgctg gattaaaccg ttgattcaat
     2761 ccttcctgac tcatcaggac acctgttgaa gcttctagtc gactacacag ttgtgcaagc
     2821 gatgtgcgag cgacttcctg gctaagccac acgcaaagag ccatcagttc atttgcttga
     2881 tacttactcg atcgtttcac aaatcccact ttcttagcga tttcttgtaa ggcaatggga
     2941 gacaaagagc gttgtaattc ttgtgcaaat aggtgtaatt cttcggaaaa ctgaacatcc
     3001 ataaaaaaac gccatccttt tcgagtagtt tactaaaaag aataacgttt ttttcgtttt
     3061 ggggaaaatc cattgcgtta gcttgatggg tatatgtctg taccccaata tatagactta
     3121 tatacatatt atataatttg tcataatgga ttttttcgga acacatataa aagcaaactc
     3181 ttgatatttt attcaattaa taatgacttt tggtttacaa agtcaacaga ccgtgtataa
     3241 ttgttataac agtgataacg taaaattata cgtactgtta taaaatcgcc agcatatgtt
     3301 aatactatgc taatactaag gaggtattat tatgatggat atggcggtga gagaagtgga
     3361 aagaaaaatt acgaaaattg gcaatagctc tggagtgaca tttccacctg aagttttaga
     3421 acacttaaac gttaaacaag gtgaaatggt tgtgtttgac cttgaaaaag acggtaaagt
     3481 agtacttaaa aagcgacctg cggtaaatgt agaaggtttc gaggaaatag atgcggattt
     3541 tttagaagga atgaaggatc tatttgatcg atacgataat acgctacgta acctcgcaga
     3601 tcgttaaaag aaaaacaaag ggtaagggac ttagcaaatg acgaaatatc tcactcataa
     3661 gcaagccatt ttcttaaatg gtgtaatgat tcaacgttat acacctaaag aacatattgg
     3721 tgtaaaagat gaaaaattat tggatagtgc gattatgcgt ccacaaagtt ctgcttttgg
     3781 atctgatgct tatcctagtt tggaattgaa agctgcagca ttgtttgaaa gcttagcaca
     3841 aaatcattct tttcataatg caaataaacg aacagcattc gcttgtttat tcctattttt
     3901 aaggacgaat ggttggattt taaatgttcg agaaaaagaa gctgaagaca tgacttcttt
     3961 tgttgtgaca aacaaacctt cattacagca tattgccgat tggatttcga gtaatcttgt
     4021 tcaacgttca tgaaaaagac cttttccaaa acagaaaagg tctttttaca gttatttatt
     4081 taaatgcagt taacataaaa ccaattatcg gtagcatttt aatctaatcg attacattca
     4141 taagaacttc acagtcacaa aaaccacctt ggttttgcag agcctttaca actttagctt
     4201 ttttgctcac ggagatgtgg gtatctagcc attctttagt gtagagaagt gtatggtcac
     4261 acccctgtat gtccactttt tcgtccagaa atgcttctaa tgaacccagc tcctcaggtg
     4321 ataaatcaag ttcgtgccac ttatttgctt gctcttttag tagcactaac ttctttacta
     4381 actttctgtc ttttttgtta agagtgactg cattttgaag tgctgataaa gctgctttat
     4441 aattcagtag atattcttca gcttgagaca acaactgcca agcaaacgca tctgttggat
     4501 gctgttcaac atattcttta agtccttttc ttgctgctaa aagatgggga attgcttttt
     4561 tagaaggagc tcttctttga taagatcctt gcattgctag tttttgtcct tttattagtt
     4621 catcttttaa ttctaataaa ctatccattc aaatagctcc ttttaaacag tattaagaaa
     4681 ccaaaaatcc tttatgtgat tttcccataa ccttcaatat tcttcaatta ctttatgtat
     4741 actctatgca tttctagtta acataatgtc tctttccgga acacatgaaa aaaacgagcc
     4801 cttgtgtatc aagggctcgt ttgcttttta aatttatggt ttatacagct ttttatacat
     4861 cgttgataaa tcagcgtttt atctcgaggt gaataaaagc cgaatgtata aaaagcgcac
     4921 tttttataca tatgcagcaa cctcgtccat tggccaatgc cgaggttgct ctttttgttt
     4981 tacttctttt tcttcttatc taaaagtgag cctgcaattg cactaaatac atcgcctatt
     5041 aaagacaata taatggccat ttattatctc acctctttta gaagattttc ttatattcat
     5101 tatatgtttt ttataaccca atgaataata caaagtttaa gatttccaaa actcccacca
     5161 ctttttttct tgagctgctg ctatcatttt tttggtatct tgaacttcac gaattagaga
     5221 taaaagattt tcatctcttt tatttgaact tgtttcaaca tttgttttta attcttcatt
     5281 ttgatcctgt aaaatcttat tttgagtttt aatatcttta agctcttttt ccattgaatt
     5341 taatctatca gatatttcct caatgtattc taatttatcc atgagaacag aaagttgatt
     5401 gttctctttt tgttcttcag attgattttc ttgaggaatt actttgttct cctcaagaac
     5461 agtacctgtt ctctcctctt ctggaagcat tgataacact acatttacag ctgtttccat
     5521 agtcatactt ttatcttgaa ctagatcttt cattctttgg agtaaaagaa tatctttact
     5581 tctaaatgct ctagaattat tcgaccccct gatgaattta taatccttat tttctaatgc
     5641 aatacaatat tttgtaagag aactacgagc tattcctaat gattttgcta cctctccacc
     5701 caaataaact atttcattta tttgttcaga catatgttca ccacctattc ttaatacata
     5761 taactattta ttatataggc tattctaaca atgttttcga tttagaataa tattctcctc
     5821 tttattctct aatcaatata taagcttaac aagaggagtt ctattaaata ttatgtttat
     5881 acaggtattt acaaggttgt tcttatgaag aacaagagtt gttctactgt tcattagaag
     5941 gtttaatata gctctaaagc tggccgtctt aagacggctt ttttagcctt taggctttta
     6001 tttttctaac gaaaaaatat aaaaagaccc ttgcaattcg agtggcgaat tgttattaaa
     6061 tatagagtga gaagagaaat aaaaaaaaaa cccttataaa cccttatata tcaaggttta
     6121 taagggtttt cgtgtgtctc gttttcgaga cacttgcgtc tcgaaaacga gacacacgaa
     6181 atataaaaaa aacgcagtga tcgagaataa tcactacgtt ttctattaaa ataatttatc
     6241 tggtaaatct tttaggactt tcatcttcat tgctttatga aacatgacct ttaaagcctc
     6301 atttacatta tctttatcac ctgcatatat aatatgcgga tttacaaaga tagcataagc
     6361 ctttgcatta ttaccttcaa ctcctgaatc tcctttaaat aataatcctt ttttaaccaa
     6421 agaattaacg gctaaactag tattctgacg cgtaatacct attaatttag caatctcaga
     6481 aatattcgca ggaacaggat ttttttcttt gatatcatga acaatacagt tactcgaaaa
     6541 agctatgtat ggaattatat cagtgagaaa tactttttct ttattagtta catagtcttg
     6601 tttatataag aaccctaaat tattttgcat agattgagca aacctcactt tcgattgagg
     6661 agaacgttta gttcctacat aaagatcttt accagttgct ttacttaata agcccattgc
     6721 ctcttctaaa tcttcttttg taactcctcc atgatccaaa gcatacttaa ttttctctaa
     6781 atcaaaatct ctactgagag catttttctc agcctcttta aaattaacga tgtttttagc
     6841 cacttttaaa ttcctccaat ttttgtagaa ggaatcaacc taattatgtt ataatataat
     6901 tatcacaata taaataacag aaggttaacc ccttcttgta tatttgctta gaaaagtcct
     6961 cctgtcatcc aaaacttgag ggctttttct tatgtctaat tttctaccaa atctttcata
     7021 agtgcaacct tatttcgagt ccattcgatg ttttaacgct gtaagcagct tatcatccac
     7081 tcgcttaggc ttatacccca tccggtctac caaaagaccc tctatgagct tcatacgggc
     7141 cgtataaagg cgatattcgc tttctccgaa ctctatgata cttttatcaa tctcaatccc
     7201 ctctggcgct ttctgaaccg ttctcattaa attgtagctt tttgaacgct cttctctaac
     7261 caaccgaagt aagattaaca agtcatacgt atgaaaatgt tgaagctctt ggaatgacaa
     7321 agccgaatct tctaaactca catattcttt aagttgagga cgctttgtat ataaaggtgc
     7381 tccacaaact gaacatgctt gatctataga attattttct gaatcacaat ataaacattt
     7441 catcataggc ttctacgaat ccttactctt cagaaacagc cacttcagct gctgctttct
     7501 ttttttcttt tacaaccata tcatgattca aaatcttctc tttatttttc cctacatacg
     7561 cttgcaatgc cttcgcaatc ttttcagctt cttcttcact ttgaatctca aagtatttct
     7621 cgaagatcgc gccgacttga ataagtcgtc ttgttctctc ttttctctct ttttcttttt
     7681 tacgcgattc aacttgttgt ttctttgctt tcatcttctc aattttctct tctaactcct
     7741 gaagaatttc ctcatctgtt cgcttcttcg gcatccatta tcatctcctt tttctgttcc
     7801 ctaatgtttg tatcgattat atcaaaaatt gagataaatt ggtatcctta gcatagattc
     7861 tctcaccagg atatggtata attcccttag accgaagaga taagggcgca cttatacatc
     7921 gtaccgatgt gtgcggttcc ttcggaacga aaagacaaaa ccaaagaccc attcgcctag
     7981 atggcgaatt tcataaacct tgtttgccta tcggcaaaga tgaaaccacc atctgacacc
     8041 tttggtgttc atgatgctga tttcatctaa agcattgatt agcgcacctt gtgctctcat
     8101 caacttattc caaaaacaca aacccattct caagaaagga ggaaagaatc atggcaattt
     8161 accatctatc gatgcaaatc atcagccgtt caaaaggcca atcggctgtt gctgcagctg
     8221 cttatcgcag tggcgaaaaa ctacatgatg aacgcacaga cgagcaaaag ttttatgctc
     8281 gtaatgtaca accggaaacc atgattctga ctccttccaa tgcgccagaa tggatgaaag
     8341 atcggaaccg tttatggaat gaagttgaaa aagtagagaa gcgcaaagac tcacaattag
     8401 cacgcgaatt aaacattgcg ttaccaattg aactgaatca tgaccaacaa aaagaactca
     8461 ttcgatcttt tgctcaaaac gagtttgttc aaaaaggaat ggttgctgat attgccattc
     8521 atcgtgacga tgctaataat cctcatgctc acatcatgtt aacgatgaga aatcttgatc
     8581 aggatggctt tggcaaaaag aatcgtgact ggaatgctga ttttgccaat gcaaaagaaa
     8641 acaatcgtgg gtacgtcaaa aatagcgaag gctgcttatc cattcgtgaa cagtgggcaa
     8701 actatgccaa tcaagcatta gaacaagccc aaagtaaaga gcgtattacg cacctttctc
     8761 atgaaaagcg tggacttgaa gtccttccaa cgattcactt agggcatgta gctcatgaca
     8821 tggagaagaa agggaaacaa agccaacgcg gccaaataaa ccgagagcgc caagagtata
     8881 accaagtcgt tatagaccta caggattacc gccgccaaaa agaggctcat ctacaaaaaa
     8941 tgaaagaaaa agagaagcaa tttagcttct ctacagatgt tgagaaaacg cagattcaaa
     9001 aggctgcatc tcttttgaat caaaaaatcg tttccttaga cgatatctca aaacgccagg
     9061 cagaactacg caaaatgagt gatcgtcatg acccgattga acgtcatttc catgtgcagc
     9121 aacaacagtt cctaaatgtc agcaattact atgaccgtgt gaaggctttg gagcatgata
     9181 ttaagcagaa tgaagagaaa gtagatgaac tcaagggatc actcaatccg tttaaactga
     9241 aagaaaataa tatgatgaaa cgtcgttacc ttgataaaat ctctgattta gaaggcatta
     9301 aagcaaacta tgaaagcagc atcaaagaat ataaagaatc ccttcgtttt tcaaatgaag
     9361 aagaattcta ccaacgctat caagagtttt ctcaacaaaa agaaaatcgc cttcatcaaa
     9421 tcaaatatga gaaacaacag attcgagtcg aaacaaaaac gttgttacaa gcagaagaag
     9481 ccgttaaaat ggctaaaatt cgtgaagtaa gctcacatta tcccgattta aaaacagctg
     9541 gaacatactt gagctatgac aatgccttaa aactagagaa acatatagat atggccaaaa
     9601 agacgccagt cacattagaa atcattgaaa aagcgattgg aaatcaaaaa gatgagttgt
     9661 acaaactaaa gaaaaatgta aaaatctttg aaacagaaag agattattta agtaccatga
     9721 agaatcaagt tacgaacctt gaaaaaacgg aaaaagaact tgatcaacta agtaatagtc
     9781 ctggtgagac agctaaacga ttggtttcga aaaaagcacg tcaacattac gaagaattac
     9841 agcatgaagc caatcattac cgagagaacc ttaaagaaaa aggatataaa ggaccggatg
     9901 atttagagaa gcgacaaggc gaaattagag agcttgagaa aaatgtaatc cccaatatag
     9961 aaaagcaaat aaacaccaag gaacaagtca taggaacgat ctcaggcatc tttgatgcca
    10021 ttcagcaagc tactcgtagc gaagagcaag cacaacaaaa gcagcagcaa cagcttagaa
    10081 gatttagaag tagctccata aataaaccca aagatcaagg tcatgacttt tctcgttaaa
    10141 aagacaacta attgccttaa ggcaatttga tcaacttaaa gttgttattc tctaatataa
    10201 cattgaccat ctttttcaaa tgaacttaaa agagatggtc aattacctat taaaaaggtt
    10261 tttatagttc gatttactct atgcttttaa tctcttcatc tctaggatta atatcttcaa
    10321 agccttcaat acataaaaca acactttcta ttgccataat atttaaacat agaaagttat
    10381 taatcaaaag tgtatagcaa tttttaatat tttctttcgc taaatagcat gtagagtcac
    10441 ttatagttag tttacattca atttttgatt tttctgttac acacatataa acagaaataa
    10501 tatctttaaa ggggatttca cataagaaaa ccccaccatt ttttttctta aattctacaa
    10561 gaaatactcc ttctattaaa atattaattt ttcttaatgt cacccacttt tcatgtatta
    10621 aaattacttt attcttgaat tttgttttat ctgaaatttc tttgcatttt acctgacttt
    10681 catctaaata gcatattggt tgacatgaaa aatcaatttc tgtagttgga atttcgtaat
    10741 gtatttgaat agaaaacctt cctttatatg ctgtttctgt atcattaata atagagatgc
    10801 tactaatatt actaaaagta aaattaaaag tacctttata aatgggaaat tgtttttcat
    10861 ctttttcgcc tttattgaca cttaataata atttattggt actacattca gtaatagtta
    10921 gactaagtgt acaagaaata ctttccatat tgattgtttt ccacagacta taaagggaag
    10981 gtttaaactc tgcaatagaa aattcttgac aaaacacatc atgaacgggt gctttttcta
    11041 gaatactttc tctcattttt aactctatag gtttttgaac ccaatcacat attttatcag
    11101 cagaaatagc taattcttct ctatttgata tatagtattt gtccaccttt atccctcatt
    11161 taaattcata taattattta tttatctttt atacatattg taaaaataaa ttaaacatga
    11221 gaataattaa gcaccaatat cgtcaataaa gcatctaata tttcaagtga acatttgaaa
    11281 aaacgaggtg aatttaaaat gtcttgcaat tcagattata attcagctat tttaggtaac
    11341 tgttctagag aaaatgtaaa agaccaatta tgttgccaat tcagtatagc tgcaggagca
    11401 gatgaaacaa tatatgaggc aattaatgaa acagttatag cttctttcat tttgaaaaat
    11461 tcaggtacgt ttccttttga tgtgacagca accaattctg caggtactac ggtattaact
    11521 gaaacgttag ctccaggtca atgtttaatg agaactattg acaatttagg agctatttct
    11581 atagcaggac ctgtagcccc tgccggtcct gctaagggag aacttgatat tgtagttaga
    11641 tatcaattag actcataaaa aaatgcttat ggttgtcaaa accataagca tttttttatg
    11701 agtcatcaat cacatataca atttgatccc gaacaagatt tacaaataac tgttaatact
    11761 tgattactaa acgtgatttt accacaacta aaagtgataa atgatttatt cctcaaaaaa
    11821 catacacatt ttaaaatttg aacttcgaac acaatagtaa cagtttcatc aacattgata
    11881 tcaggaagtg aaataccagt ctcaggattt actccaggtg ttgacactcc gtttataacg
    11941 acactgtcat taataaaaac gctaccttca ggtattaaat cggtaaatac aacattatta
    12001 gctggaacta caccttcatt tgtaatagta attgtataag ttaacgtatc taaaatatca
    12061 gccattgtag tattaaccct tttttgaaca tctaatacag catcgccttg atcaatttga
    12121 actcctaaag catttggcat atacgcatca ccagtagatg tatatctaaa tgttgcaatc
    12181 atttcattgt tacctagata atttgtagcg tctacattag ttata
//
