LOCUS       NZ_CP069299            10124 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Bovismorbificans strain
            GSJ/2016-Sal-017 plasmid pSal017-2, complete sequence.
ACCESSION   NZ_CP069299
VERSION     NZ_CP069299.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14309717
            Assembly: GCF_016839205.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Bovismorbificans
  ORGANISM  Salmonella enterica subsp. enterica serovar Bovismorbificans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Li,L. and Meng,H.
  TITLE     Comparative genome anaysis of Salmonella isolated from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10124)
  AUTHORS   Li,L. and Meng,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) Food Safety and Nutrition Institution, Jinan
            University, Huangpudadao, Guangzhou 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. Dec-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016839205.1-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 11:17:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,800
            CDSs (total)                      :: 4,678
            Genes (coding)                    :: 4,550
            CDSs (with protein)               :: 4,550
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 50 of 128
            Pseudo Genes (incomplete)         :: 88 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 29 of 128
            Pseudo Genes (short protein)      :: 1 of 128
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069299.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Salmonella enterica subsp. enterica serovar
                     Bovismorbificans"
                     /mol_type="genomic DNA"
                     /strain="GSJ/2016-Sal-017"
                     /serovar="Bovismorbificans"
                     /isolation_source="Qingyuan chicken"
                     /sub_species="enterica"
                     /db_xref="taxon:58097"
                     /plasmid="pSal017-2"
                     /geo_loc_name="China:Guangzhou"
                     /collection_date="2016-05-30"
                     /note="6,8:r:1,5"
     gene            join(9759..10124,1..30)
                     /locus_tag="JQ707_RS24160"
     CDS             join(9759..10124,1..30)
                     /locus_tag="JQ707_RS24160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_239005549.1"
                     /translation="MVNVEFFNYIECIFMRIKKPHLRPIVITLSVGGILLTSIFLIVVI
                     MVFKRENIEKDLLDANSSYAMKMSDVMSNYIEIAQRELAYGAKKIESTNDIDSLKNEAD
                     RLRLQSGMFNSVLVVSDGTHLKMRRIG"
                     /name="hypothetical protein"
     gene            45..1088
                     /locus_tag="JQ707_RS24165"
     CDS             45..1088
                     /locus_tag="JQ707_RS24165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="WP_239005550.1"
                     /translation="MFNSVLVVSDGAVVLATSPESLGLVGVHLNSSASKLAIEAKKPFI
                     SAPYKSASGNLIIFLSHPIYDKNSNYIGYVGGSIYLKEQSLFSDVLSRHFFKSDTEISI
                     VSDDGNVIFNNDNSAVGRPMVMDDNLKSKLTSSERGEGIFFSSGNKYLLGYAHMKNTAW
                     SVLVYSHADSVTTILITYAKSVLVLLIVIVITFSLFSYFIASRISIPLEKLAISTNAKD
                     IGGSLVDIKGINAWYVEADRLKKALFTHIKIMIERVDSLNEVALKDPLTGVNNRLGFSN
                     KIQNHKQGSGASVIAIDVDFFKKINDNFGHGVGDEVLVTLAQVINCGFPGLGRFPQSRG
                     QPERKPE"
                     /name="diguanylate cyclase"
     gene            complement(<1016..1621)
                     /locus_tag="JQ707_RS23810"
                     /pseudo=""
     CDS             complement(<1016..1621)
                     /locus_tag="JQ707_RS23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /name="ISKra4-like element ISKpn19 family transposase"
     gene            complement(1658..2230)
                     /locus_tag="JQ707_RS23815"
     CDS             complement(1658..2230)
                     /locus_tag="JQ707_RS23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001493762.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNRP
                     GLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGTNN
                     PMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALRSRG
                     VRLRIACDHAGLGVSTYYKLRHQMNNQ"
                     /name="recombinase family protein"
     gene            complement(2367..2912)
                     /locus_tag="JQ707_RS23820"
     CDS             complement(2367..2912)
                     /locus_tag="JQ707_RS23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_001493763.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGIS
                     YEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFCIS
                     GHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQLSRL
                     DLASPVLEPEVIWLGRRR"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            complement(<3048..3230)
                     /locus_tag="JQ707_RS23825"
                     /pseudo=""
     CDS             complement(<3048..3230)
                     /locus_tag="JQ707_RS23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            3392..4006
                     /locus_tag="JQ707_RS23830"
     CDS             3392..4006
                     /locus_tag="JQ707_RS23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412822.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_014839983.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKREE
                     RPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTT
                     TAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMG
                     QSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
                     /name="recombinase family protein"
     gene            complement(4325..4981)
                     /locus_tag="JQ707_RS23835"
     CDS             complement(4325..4981)
                     /locus_tag="JQ707_RS23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="WP_001516695.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFVN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRTNF
                     AHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS1"
     gene            5376..>6406
                     /locus_tag="JQ707_RS23840"
                     /pseudo=""
     CDS             5376..>6406
                     /locus_tag="JQ707_RS23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            <6470..6697
                     /locus_tag="JQ707_RS23845"
                     /pseudo=""
     CDS             <6470..6697
                     /locus_tag="JQ707_RS23845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /name="diguanylate cyclase"
     gene            complement(6940..7098)
                     /locus_tag="JQ707_RS23850"
     CDS             complement(6940..7098)
                     /locus_tag="JQ707_RS23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001658417.1"
                     /translation="MHEPPVQSDCCASSGNFQLDSNPERHDKAPLAAAIGNWELVDLDI
                     ESLEVVA"
                     /name="hypothetical protein"
     gene            7748..8071
                     /locus_tag="JQ707_RS23855"
     CDS             7748..8071
                     /locus_tag="JQ707_RS23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015059464.1"
                     /translation="MLTIWVTEDEHRRLLDRCDGKQLAAWMRQTCLDEKPARAAKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8280..>8714
                     /locus_tag="JQ707_RS24170"
                     /pseudo=""
     CDS             8280..>8714
                     /locus_tag="JQ707_RS24170"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8749..9234
                     /locus_tag="JQ707_RS23865"
     CDS             8749..9234
                     /locus_tag="JQ707_RS23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012482081.1"
                     /translation="MSSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELNESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSVLLIASSAGILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRSCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            9238..9453
                     /locus_tag="JQ707_RS23870"
     CDS             9238..9453
                     /locus_tag="JQ707_RS23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     ARG             complement(4329..4981)
                     /name="qnrS1"
                     /gene="qnrS1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6932..7475)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.788"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          8062..9557
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.583"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actcacttaa aaatgaggcg gataggttaa gattgcagtc tggtatgttt aattcagttc
       61 ttgttgttag tgatggtgct gttgttctgg cgacttcgcc agaatcactt ggtttagttg
      121 gagttcactt gaattcaagt gccagtaagt tggcgattga ggctaaaaaa ccatttatat
      181 cggcacctta caaatcagct tctgggaatt tgattatttt tttgtcacat cctatttatg
      241 ataagaatag taattatata gggtatgtcg gcgggtctat atatctaaag gaacagagtt
      301 tatttagcga cgttttgagc aggcattttt tcaaaagcga cactgaaatt agcattgtta
      361 gtgatgatgg aaatgtgatc tttaataacg ataacagtgc tgttggtcga ccaatggtta
      421 tggatgataa tttgaaatcg aagttaactt cgtctgagag aggtgagggt atcttttttt
      481 cttcggggaa taaatatctt cttggttatg ctcatatgaa aaatactgct tggagtgtgt
      541 tggtttatag tcacgctgac agcgtgacta ctattcttat tacttatgct aagagtgtac
      601 ttgttctgct aattgtaata gttattacat tttcactttt ttcatatttc attgcttccc
      661 gtatttcaat tcctttagaa aagcttgcta tatctaccaa tgctaaagat atagggggtt
      721 cgctagttga tattaaaggt atcaatgctt ggtatgtcga agccgaccga ctaaaaaagg
      781 cactttttac gcatattaag ataatgatag aaagagttga ttctctaaat gaggtggcgc
      841 ttaaagatcc cctaaccggc gtgaacaaca gactggggtt ttcaaataaa atacaaaatc
      901 acaagcaggg ttcaggggct tcagttattg ctattgatgt tgattttttt aaaaaaataa
      961 atgataactt cgggcatggg gtcggagatg aggttcttgt tactttggct caggttatta
     1021 attgcggttt tcctggcctg ggcaggttcc cccagtcgcg ggggcagccg gaaagaaaac
     1081 ctgaatgagc ttcagcctca gcgtcaagac gctgcgccac ctggcataaa tgattcctca
     1141 ccgttgaggc attcaggctg tggcccaccg gcagaatatc tttcagcaga cgagtcgtca
     1201 tttcatagga gatcatcgag gcccagcggg tttcaatata tttcagtgcc gggtgagaat
     1261 aatcgccggc ccagtcgctg agcaaactga ctgtctttgt atcactctct tcacaacggc
     1321 accggtacac ccgaagcccg gataccggaa taacgccaaa cagcgtccgg taccgtattt
     1381 tctgtttgcc tttgattctg cgcgcagcaa ggcagtgagg gcaccggata tggtgttgag
     1441 tatattcgtc tgcctgcagc tggaccaccg actgctggac cgtattcagc aacagcttgg
     1501 attctgacac cgataatccg atgtcgttcc gggtctcccc tgatttctgg attgtcatca
     1561 gttcctccgt tcgactggag cctgattcat cggtgatgac tatctggagc gtcagttgca
     1621 tcattgcctc ctgctgtggt ggatgactat ctcccggcta ctggttgttc atctggtgac
     1681 gaagcttata atatgtggaa acgccaaggc ccgcatggtc gcaggcgatc ctgagcctga
     1741 cccctctgct tctgagtgcg tcaatttcct ggatcttttt gatatcggcg ccggggcgcc
     1801 ctgttttttc accccgtgct tttttcgcgg cgatgccgtc ggcctgcctt tccttcagta
     1861 aggctcgttc aaactggctg aatgccgaca tcatgtgcag ctgcagttcc tgcatcggat
     1921 tgtttgtgcc ggcgctgaac gtcagctgct ctttcagaaa aataagggta acgccctggt
     1981 cacgaagccg ggtggtgaca gcgcacatat ccgacatatt ccggcaaagg cgatcgatgg
     2041 aatgaaccag aagcgtatca ccagggcgta attgccccag gagttcctgt aaccccgggc
     2101 ggtttgtgtc cttcgcgctg gcatgctcaa cacaaatttt atcaggctga aaacccgcct
     2161 ttgacaatgc ctcttcctga cgggccgtat tctgatcggc cgagcttacc ctgatgtagg
     2221 catattgcac gcaaaacctc cggaaggtct ttaaacatca tagagattta aagaataatc
     2281 cataaacact gatttaggga ggtttataga cattttttcg gggatctgag aggcatgctg
     2341 aaaacgtata cctttataga catcagtcag cgacgacggc ccagccagat aacttcaggc
     2401 tccagtaccg gagatgcaag gtcgagcctg ctcagttgcc ggttgatttt gtggcggttg
     2461 aacctgacag catccaggtc gtcagcaaaa gggcggatct cgccgaccgt tgtttcatca
     2521 tccgcattaa cgatagcttc aagaaacgcc agggttttat cgaactcatc cgcagctgtg
     2581 gctccgggca taccatggcc gcttatacaa aacggcgctt tcagcgtaga gttttcataa
     2641 atggcctcaa cacgaatgtc atgaagccag tgctcaaaga aattgtattc ataggtaaag
     2701 cgatcgccag catcaaaagc aaaatcatca atcacgaccc ggaacggatt atcgacaaaa
     2761 ccgatgcctc cttcgtagga gataccgtaa tctttgccat aaatgtgaaa ttgatggaga
     2821 tggtcgtccc cccagccctg cactatctgg aaaatgaagt gaagcgcggc cagtgacgtg
     2881 tcggcagcaa ttctcagccg acgccagacc atcggactga ctccatgaac tgctattttg
     2941 atgacgtaga tcttcatagt cagcaggtat tgttatgaaa tgatggtggt tattttaacc
     3001 cctgtagatg gcatcattcg atggctgccc cgaggttttt catgctctcc gatctggatg
     3061 atgcaataaa tttgagcgtg aagcccccgg cgactgcagg ccagcgccag ttcatatcca
     3121 ttcagcgcca gaaactccag ccgctgtgca tcatcgaaat caggtagacc atagagcgct
     3181 tcctgctcgg cgtccgaaag aatactgagg agcttatttt tagtaggcat ctctgatcct
     3241 tatgcctatt taaacgcgct tgtcacatta tcattaacat agaaccttta cgtgtcataa
     3301 tttaaattac gggtaatgtg actaccgttg acagattatg gtacccaaat cagaatgtca
     3361 cataatcgaa cgtatatgtg acagaggaaa tatgttcatc ggatatattc ggatgtcaaa
     3421 agctgatggt tcgcagacga atgatttaca acgtgatgca ctgatcgccg caggtgttga
     3481 tgccgcacat ttttatgagg accgggcatc gggcaaacga gaagagcgtc ctggacttac
     3541 agcctgtctg aaagcactac gacccggaga cacgctggtc gtatggaagc tggaccgcct
     3601 cggtcgtgat cttcgtcatc tcattaatac agtgcacgac ctgaccgcga gggggaccgg
     3661 tctgaaagtg ctgaccggtc acggggcgac tatcgacaca acaacggccg ctggcaagct
     3721 tgtcttcggt atctttgccg cactggcgga gtttgagcgc gaactgattg ctgaaagaac
     3781 gactgcaggt ctggcatcag caagagcccg tgggcggaac ggcggccgac cttataaaat
     3841 gacgccagtc aaactgcgac tggcaatggc atcaatggga caatcagaaa caaaggtcag
     3901 tacgctatgc caggaacttg ggataacccg gcaaactttg tacaggcata tttcccctgt
     3961 tggtcaactg cgggcggacg ggatcaagct gttcaaccgt ggataatttg aacggacgga
     4021 aaaatctggg gttccggcgt agtaccccta tcacctccac atttttgcaa cagtgcctta
     4081 taaatggcag ccttatgttt acttgccaag atgggctacc atatgatgtt ttatactgag
     4141 caatattaat attatcttta atggaaaaat taagcccact aagtgaccca ctgctatatg
     4201 gctctattgt gaagacgcct gagggtaaaa atttatacat ggttgtccct atgtctatta
     4261 ttgcaaggtt gacaatatta ttcgttttat aaatttgagc gtttaaaatc acacatcaaa
     4321 gcaattagtc aggataaaca acaataccca gtgcttcgag aatcagttct tgctgccagg
     4381 ctgcaatttt gatacctgat gtatcgactt tgcggggatc taaaccgtcg agttcggcgt
     4441 ggcataaatt ggcaccctgt aggctaaatt gcccccagac atcttcggaa aaaacacctc
     4501 gacttaagtc tgactctttc agtgatgcac ccgctaggtt cgttcctatc cagcgatttt
     4561 caaacaactc acatttttct aaacaaaccc tctccatatt ggcataggaa agattacatc
     4621 cagaaataaa tgctgagcaa aagtacatac gattactcac ttgatgggca aagtttgttc
     4681 gggaaaagtt ggcacctttt aaatcacacg cacggaactc tataccgtag caattggcat
     4741 tactgaagtt tgccatcgca agttggcatt gttggaaact tgcatcacga agatctgcga
     4801 catcaaagtg gcagccttcg atatcaccct gttcaatgaa cttgcagttg acgaatgtcg
     4861 tatcacgcaa gttagcacgt cgaaagtcgc tgcgaatgaa tgtgcaagcg gtgaaggtga
     4921 gatcacttaa gtctttatgt gaaaagttgt ggtgccgata tgtatgattg taggtttcca
     4981 tgagtactcc ttgttgaaag gagggctaga ctaccattat acatatgcca agacattaaa
     5041 ataaaagcct gttacatcaa cacattacaa cccctcaatt tacctgtttt aagtggtcaa
     5101 attggcgcgt agagcgccta tcgctcttgc attggcacgc ggccctgttg aaaaaagtta
     5161 ttcgctttag atagggttag gagagccgtg cttcgccggt aaaaaatcgg actatccgac
     5221 gcagggcgga ctcatcggcg gagaagcaaa ctttgactat atctccctac acttggaaga
     5281 gggctagact ggccccatag atcaccagac acttcgactc tcttaaaata agaggtagtc
     5341 ttggaactat gtttaatacc agtcagaacg ataagatggt tgaagtgtta tcaggacctg
     5401 agcggcgtcg gcgtcgtaca ccgcaggaaa aaattgccat tattcagcag actatggagc
     5461 ccggtatgac cgtgtctcat gtcgcgcgcc tgcatggcat caatgcaaac cagatattca
     5521 aatggcgcaa gcaatattaa gacggttcac tgacggctgt tgcatcgggc gaggaagtgg
     5581 tgccggcctc cgaactcgct gccgccaata agcaaattcg cgaactccag cgccttctgg
     5641 gcaaaaagac gatggaagcc gagatactca aagaggccgt ggagttcggc cgggcaaaaa
     5701 aatggattgc gcatgcgccc tcgttgcctg ggaacgacga ataagcgacg tttgcagaag
     5761 tcttggcgtg tcgcgtgcgc agttgagcat tcgggttcac cggccatctg actgacagga
     5821 tcgcagacgg cagccccgtt ctgacgatac caccgtattg tcccggataa acatggcggt
     5881 ggctgacctg ccgacgtatg gttatcgccg tgtgtgggcg ctgctgcgac gggagtcgga
     5941 gcgggacggc ctgcctgtgg ttaatgcaaa gcgggtgtat cgcattatga gaacgcataa
     6001 tctgcttctt gaacgtaaac ccgctgatcc ttgccgtaag cgggcccata aggggcgcgt
     6061 agcagtagcg gaaagtaacc ggcgctggtg ctcagatggc tttgagttcc gctgtgataa
     6121 tggcgaaaag ctgcgggtga ccttcgcgca ggactgctgc gacagggaga tcattgattg
     6181 ggcagcgagc accggaggct atgacaaaga aacggtgcag gatgtcatgc tgggtgcggt
     6241 ggaaaaacgc ttcggaaggc agttgccaac agtgccggcg ttcgcccggt tgctgggact
     6301 ggagccttgt acgacagcag tgcgaagccc ggaaagcaat ggcatagcag aaagcttcgt
     6361 gatgacgata aaacgggact acatcagcat catgccgaaa ccggataatt cttgctgccg
     6421 tagtgaagat attgtttgtc ggtttggtgg agaagagttt gttgtttttc tgccgaatac
     6481 atctgtggta acagcggagt gtgttgcgga gcgtattagg agtgttattg aaagtactgt
     6541 tttccctaat aacttgcggg tcaccgtctc tgctggtgtg gcaacacaag aggatcctct
     6601 tggtgggatc gattttttgc tgaaaaatgc tgatgatgct ctttatcaag ccaagggaga
     6661 ggggcgtaat aaagtgattg tatatagcgc tatttgagtt tttttgatga cgctttacgc
     6721 gctagtgtgg cgtttttttg tggggaaaag ggttcaagaa gcggatcctc ttgttatccc
     6781 ttcgatgtcg tcgtaagctc atgccctgta aacaaaaaaa ccaccgctac caacggtggt
     6841 tcgtttgccg gaattaagag ctaccaacac tttcttccga aggtaactgg ctttagtgga
     6901 gcgcagatac caaatactgt cctttcagtg tagccgtagt caggccacca cttcaagact
     6961 ctcgatatct aaatccacta attcccagtt accaatggct gctgccagtg gcgctttgtc
     7021 gtgcctttcc gggttggaat caagttgaaa gttaccggag gaggcgcagc agtcggactg
     7081 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt
     7141 caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg
     7201 caggaacagg agagcgcacg agggagctac cagggggaaa cgcctgggat ctttatagtc
     7261 ctgtcgggtt tcgccaccac tgatttgagc gtcagattct gtgatgcttg tcaggggggc
     7321 ggagcctatg gaaaaacggc tctgccgtgg ccttatcgct tccctgttaa gtttcttcct
     7381 agcatcttcc aggaaatttc cgccccgccc gtaagccggt accgctcgcc gcagccgaac
     7441 gaccgagcgg agcgagtcag tgagcgagga agcggcattt ctccggcagt gcatcttctg
     7501 ctgacgcata cgaatctcat ttttcacctg ctttatgtgg cagctatgct tgatgtttct
     7561 gcgatgttac attttaagcc agtatacact ccgctagcgc tacgtgactg gttcagggct
     7621 gcgccccgaa acccgctaaa aggcactgac gcgcctgcgg cttgtccaac accgcgccga
     7681 ccgggaaata attccccgtc cgtcccggcg ttatcaggag gccggattgg cagacaaacg
     7741 cagcaaaatg ctcacgatat gggtaactga agacgagcac cggcgtctgc tggatcgctg
     7801 cgacggtaag cagctcgcgg cctggatgcg gcagacgtgt ctcgacgaga aacctgctcg
     7861 tgccgccaaa cttccgtcga tctcgccggc gttgcttcgt caacttgccg gtatgggaaa
     7921 caacctcaat cagattgccc ggcaggttaa cgccggtggt ggtaccggac atgatcgcgt
     7981 acagattgtc gctgcgctga tggccattga tgccggactt gagcgcctgc ggcacgctgt
     8041 gctggaaaag ggggctgacg atgatcgtta aatttcaccc acgagggcgc ggcggcggtg
     8101 ccggtccggt ggattatctg ctgggtaatg accgcaagcg cgacggcgcc agcgttctgc
     8161 agggtaaacc ggaggaagtc cgggagctaa tcgacgcctc gccctacgcc aaaaagtaca
     8221 cctccggggt gctgtcgttt gccgaacagg acttaccgcc cggtcagcgt gaaaaactga
     8281 tggcgagctt cgagcgtgtt ctgatgcccg gactcgataa agaccagtac agcgtgctgt
     8341 gggtggagca ccaggacaag gggcggctgg agctgaactt cctgatcccg aacacggagc
     8401 tgctgaccgg caaaaggctc cagccgtatt acgaccgcgc tgaccgtccg cgcatcgatg
     8461 cctggcagac catcgtgaac ggcaggctgg ggctgcacga cccgaatgcg ccggaaaacc
     8521 ggcgggcgct ggtcacgccg tccgcgctgc cggaaacgaa gcaggaggca gctcaggcga
     8581 tcacccgggg tttgctggcc ttagcctcgt ccggggagct gaaaacccgt caggacgtta
     8641 ctgaggcgct ggaaaacgcg ggttttgagg tggtacgcac cacgaaaagc agtatcagca
     8701 ttgccgaccc ggacgggggg cgaaacatcc gactaaaggg agccatctat gagcagtctt
     8761 ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc agcagcagag taccggcgag
     8821 atgctgaaag ccgcattcag cgagcacgag cagtctgtca gagcggaact gaacgaaagc
     8881 gagaagagaa tcagcgccgc catcctcgac cacgaccgga agctgtcctc agccatgagc
     8941 cagcgcacga aaggcatggt gcgtatggtc agccagacgt ggctgaccat cgtcctggtt
     9001 tccgtcctgc tgatcgcatc gagcgcgggc attctgtggt ggcagagtca gcagatactc
     9061 gacaattaca cgaccatccg ggagcagaag agcacgcagg cgatgctgtc cgagcggaac
     9121 agcggcgtgc agctctcgac ctgcggcgag cagagacgca gctgcgtgag ggtgaacccg
     9181 gaagcgggac ggttcggaga ggattcgagc tggatgatac tggcggggaa atagcacatg
     9241 acggagctgg aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa
     9301 aggctggacg agtgggagaa cgccttcgcg gaatggcgga cgatgtctgg tcttatgcaa
     9361 cgggagaacg cggcgctgag cgagcgcgtc acgggcttga gcaggcaggt gcagagtttg
     9421 agcgagcagc tgcgccggtt gtcgcaaggc tgaatggtat cgaggcgcac cgggagcagg
     9481 aacgggcggc gcagcaccgg aaagcgctgg agctggaacg atcgcagcgc cagcggacgt
     9541 acaacggtcc gtcgctgtaa caaaccggat aaaacgggtc tttcggcgca gatccccgcc
     9601 gaaaggcggt gccatccccc cggctgcatg gctatgcatc ttggggaaat ttatctggct
     9661 caaaaagcgc ggtttagcgg ggagtttact gccggtttta ccgggctgac gccgcattcg
     9721 cgccgaaccc gtccggggcg gtgcgggcaa gggatgttat ggtaaatgtg gaatttttta
     9781 attatataga gtgtattttt atgagaatta aaaagcctca ccttcgtccg atagtcataa
     9841 ccttgtccgt tggtggtatt ttgttgactt ctatttttct gattgtcgtt attatggttt
     9901 ttaaacgcga aaatatagaa aaagacttgc ttgatgctaa ttcatcgtac gctatgaaaa
     9961 tgtcagatgt aatgagtaat tatattgaaa ttgctcaacg tgaattggcg tatggcgcaa
    10021 aaaaaataga gtcaactaat gatattgact cacttaaaaa tgaggcggat aggttaagat
    10081 tgcagtctgg tatgtttaat tcagttcttg ttgttagtga tggt
//
