LOCUS       NZ_CP069212             2056 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Klebsiella pneumoniae strain SP968 plasmid pColpVC.
ACCESSION   NZ_CP069212
VERSION     NZ_CP069212.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16178551
            Assembly: GCF_022701055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Carbapenem resistant K. pneumoniae VSP968 hybrid genome assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. August 2020; unicycler v. August 2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022701055.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:49:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,519
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,278
            CDSs (with protein)               :: 5,278
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 50 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 16 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069212.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SP968"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColpVC"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College, Vellore"
     gene            67..1056
                     /locus_tag="IEE96_RS27500"
                     /old_locus_tag="IEE96_27420"
     CDS             67..1056
                     /locus_tag="IEE96_RS27500"
                     /old_locus_tag="IEE96_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_023569045.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIGELGDTLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESE"
                     /name="protein rep"
     gene            1609..1800
                     /locus_tag="IEE96_RS27505"
                     /old_locus_tag="IEE96_27425"
     CDS             1609..1800
                     /locus_tag="IEE96_RS27505"
                     /old_locus_tag="IEE96_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020324555.1"
                     /translation="MIVRLDNRLFEILDEMGAHVDREMFMAAYAQRKAEKENDPRHIRQ
                     RKLTRLAHDLMSESDPEL"
                     /name="hypothetical protein"
     Typing          complement(785..974)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.906"
                     /coverage="99.0"
                     /evalue="1.72e-88"
ORIGIN
        1 acaacacagg tgatacggca atgataacac gtcaaatgag ccatcgccca gtatgggacg
       61 gtgcgcatga aaaaccctga actccagaac ctcacggact attccccctc agatgcgccc
      121 tgggacgctc acagaagcgt ttctgacgac gtgggaggga tttacctgct tgcggccgaa
      181 tatgagcgct acggggcgcg tatggcctcc tgcggcggtt tactgcgttt cgggtggtct
      241 accctcaaag aaaccggaga aacgcgcctg aggctgcgtg aggcgcattt ctgccgtgtc
      301 cggcactgtc ctgtctgcca gtggaggcgc tccctgatgt ggcaagcccg tttctaccag
      361 tccctgccgc gtatcgtggc ggattacccc gattcccgct ggatgttcct gaccctgacc
      421 gtgcgcaact gtgcgatcgg ggagctgggc gatacgctga gccggatgaa cacggctttc
      481 cagcggctga aggatcgcaa agagttcaga ccagttcagg gatggatccg caccacggaa
      541 gtcacccgcg gcagtgacgg cagcgcacac ccgcacttcc acacactgat gatggttccg
      601 cctggcatgc ttaacgggaa gagttacgtc aaacatgcgc gctgggttga gctgtggcgg
      661 gagtgccttc gggtcagcta cgatccgaat gtcgatgtcc gggcagtgaa accccggaag
      721 ccgaaggacg gcgaatcgct ggcctgcgcg accgccgaac tggtgcgcgg cgcggtggcc
      781 gagaccctga aatattccac gaagccggcc gacatggtcg ccgatcctga atggttcctc
      841 gaactgaccc gccagacgca caagcgccgc tttgtggcga ccggcggcgc actcaaagac
      901 gtattgaaac tcgatcagga aaccgacgcc gacatggtga tcggggatga tatttccgag
      961 ggcgacgatg acggttcacg catcgcgttc gagtggaaaa ccgaggtcag aaaataccgt
     1021 cgctcaccaa cgaaagataa aaccgaatca gagtaacggg gtgtcggggc tgcgccctga
     1081 ccatgaggac tttgggcatc cgtcaccttg gtggcggtgt accaaagtca catcttgtcc
     1141 gattgatttt actccgatgc aggatagggc tgccgccgca aagtgacgtc actcgattgc
     1201 tgagttcgga ttccgaacct gggttttcct tccggtaatt ttccggaagg cgcgcttagt
     1261 agtttctccc cttcggtgag aaactgcgcg gtggggcaag cccacgaaaa acaaaaactg
     1321 acaccggaca agatgtgttt ttggagtacc gccatcacga tgtcggtacc aaaaacgatc
     1381 cgtcagggct gcgccccgac accctgccaa cacctcacgc cgccagatta aaatctgtca
     1441 gcgttcgccg ttcccgaaat cgtcaaacgc taaatcgcgg ggtcccgctg gcttgccagt
     1501 gggttccgat ttagtgattg acgacagcac ccgtagaata cttacagggg gtggttgtga
     1561 tcacgcagtg agcgcaacca cctgataggc gtttttgagg gggtaggtat gatcgtcaga
     1621 ctggataacc ggctgtttga gattctcgat gagatgggtg cgcatgtgga tcgggaaatg
     1681 ttcatggcgg cctatgcaca gcggaaggcc gaaaaggaaa atgatccccg gcatatccgt
     1741 cagcggaaat tgaccaggtt ggctcatgat ttgatgtcag aatcggaccc tgaattgtga
     1801 gcctcgcgtg cgcgcgaggg aaattggtca gaaaatcgcc ggattttcat gcgcgcgatc
     1861 cttgtattta tacttaaggg ataactctgg cgttaaaaat aagcctcagc ccttgtgcct
     1921 caagggtttc agcgtgattt gcctgaaaat gactttgaag ttattctgtc gggagtggtc
     1981 gcgttaaaag cgacccccgc aacgacggcc atcgttcggg ggtgtgacca cctgtaggaa
     2041 ttctattttg cgacag
//
