LOCUS       NZ_CP069211             2101 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Klebsiella pneumoniae strain SP968 plasmid pColBS512.
ACCESSION   NZ_CP069211
VERSION     NZ_CP069211.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16178551
            Assembly: GCF_022701055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Carbapenem resistant K. pneumoniae VSP968 hybrid genome assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. August 2020; unicycler v. August 2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022701055.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:49:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,519
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,278
            CDSs (with protein)               :: 5,278
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 50 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 16 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069211.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SP968"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColBS512"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College, Vellore"
     gene            complement(615..881)
                     /locus_tag="IEE96_RS27490"
                     /old_locus_tag="IEE96_27410"
     CDS             complement(615..881)
                     /locus_tag="IEE96_RS27490"
                     /old_locus_tag="IEE96_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(885..1850)
                     /locus_tag="IEE96_RS27495"
                     /old_locus_tag="IEE96_27415"
     CDS             complement(885..1850)
                     /locus_tag="IEE96_RS27495"
                     /old_locus_tag="IEE96_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1226..1457)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tcccttaagt ataaatacaa ggatcacgcg cgagaattgc gcaaaaaata accacaattt
       61 taacccccca aaacaagcac ccccgaacgg cttaccccaa ccttttccac acacccgatc
      121 accaaaacag ggaataaaaa caaaaccctg tcttacaggt ggatggcctc acttcgtgaa
      181 accatccacc ccgcataagt tttttaatcg gtggtgtcgt caatcactaa atcggaaccc
      241 caaaggggac ccccacgatt tagcatttga cgagattaac tctcggagat ggcatcatgc
      301 ttttaacgga ctttaaaaaa cggtgaacgg atgcggattt taatccgtgt ctgtgaggcg
      361 ttgagcaacg agcaaagcga ggcggtagaa agagagccag cggcaggctc aggacgataa
      421 tccggggaaa atgtaccact cgcagagtgt taacatttga catcggattg tctatcctgc
      481 attggtgtcg agttggcaag gctgaggata atgctgagga taatgctgag gataatgcag
      541 gatagacaga gcactagaga ttgcatgcaa tctcgtacct atcacctcag cctgccgctg
      601 gctcttttcc agaactaaga atgcgttctt aaaaatccaa acttgatacc gtttaaatct
      661 attttcacaa aaagagtttt taccctctcg tttgttttct gaatatataa aattaattca
      721 tcctcaaaac cttcgtgcgg atgaaaacca aatgtgccaa aacttaatcc atcaataagc
      781 aaaataaggt ttttaaacct gtcaggaatc accgcttcac ttcttaaaaa aaataaacca
      841 atagtcttat ttccattcag gtattcaata ttaatattca tacgttaatc cgatttatcc
      901 ttctcagggg cgcgtttgta acgccgttta cctgaatccc agacaaacgc cgtccgcttc
      961 ccgtcatcag tgccatcccc tacatcgtcg gcaatgacaa gatcctcatt ggtttctcga
     1021 tccaactgga ggacgttttt tagcgcccca ccggtcgaga taaaacggcg cttgtgaagc
     1081 tgccgcgtca gctcaagaaa ccactcagga tcgtttgcca tatcttccgg tttaacggag
     1141 tatttcagcg tttcagcaac cgcgctttgc agttgctcgg caacgttggc cacaacctca
     1201 cctgtcttag tttttactgc ccgaatatcg atattcggct catagttcac ccgcaagcaa
     1261 tcgcgccaga gttctaccca acgttcgtgc ttaacgtagt tcttcccttt aaaccaagaa
     1321 ggttgcacca tcagcagaca gtgaaaatgc ggatgtgcgc tgccatcctt acctcgcgtc
     1381 acctccgtag ccctgatcca cccctgaaca ggtgatagct cctttcgctt ttccattcgc
     1441 ttaaacgccg cattcattgc tgtaaggact gttccaagtt cacctatctc gcagttcctg
     1501 acagttaacg tcagaaacaa ccatcgggaa gacgggtaat ccacaacgat tttcggtaga
     1561 gcctgataaa aacgggcttg ccacatgagg gttcttctcc actggcagac agggcaatga
     1621 cggacacggc aaaattgcgc actacgaagc cgtaggcgcg tttctccggt ttccatgatg
     1681 gtagaccaac caaaacgtaa caaaccgcta caggaggcca tacggagtgc atagcgctcg
     1741 aattcagcag cagtctggta gatacccccg acagaatcgg taagcgtgcg ctgggtatcc
     1801 caaactgcat cacgggggct gtagtccgaa aggaatttat cttctgacat taagcctgct
     1861 ccgatctggg caggctgtga tttgatgtgg tatattcatc acgtacctgt gcattgcgag
     1921 ccagtacatg tcaccagagt ttgccgctga ggtgacacat tcgaaatgtt ggggagtgtt
     1981 gacgcactcc ccaacgccga ttaaaaacgc ctcaattctg ctccattttc tttgacctgt
     2041 caaaacccac tcaaagcctc gtcattactg ggctaaacca ctatttcata tccgccattt
     2101 a
//
