LOCUS       NZ_CP069210             4237 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Klebsiella pneumoniae strain SP968 plasmid pSP968_6.
ACCESSION   NZ_CP069210
VERSION     NZ_CP069210.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16178551
            Assembly: GCF_022701055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Carbapenem resistant K. pneumoniae VSP968 hybrid genome assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. August 2020; unicycler v. August 2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022701055.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:49:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,519
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,278
            CDSs (with protein)               :: 5,278
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 50 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 16 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069210.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SP968"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pSP968_6"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College, Vellore"
     gene            632..1147
                     /locus_tag="IEE96_RS27465"
                     /old_locus_tag="IEE96_27385"
     CDS             632..1147
                     /locus_tag="IEE96_RS27465"
                     /old_locus_tag="IEE96_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000097059.1"
                     /translation="MSSLSLQRYETNPFLELMTIPMKGKRIQVSRLGRDDNVLVNQNTG
                     EKFGTHVTTFKRVDSEQFVKLFTANIGLTFDLSSAGIKAFNVLLWAVQKQAFAKDQVLL
                     DSRALDDFLKSYDSLKLSYATLKRGINELEKAQIIAKTMRKGFYFINPNFVFNGDRIAF
                     TTLIERDC"
                     /name="replication/maintenance protein RepL"
     gene            1210..1617
                     /locus_tag="IEE96_RS27470"
                     /old_locus_tag="IEE96_27390"
     CDS             1210..1617
                     /locus_tag="IEE96_RS27470"
                     /old_locus_tag="IEE96_27390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_126709166.1"
                     /translation="MSKNDVFACACRRLLEVVAGEQKWCFVRLLITTTWLEVFMTKHDA
                     IRISQLHQIFYDDDGLIDSEKNVTILYSIGFTVDEIAHFRNSTPNTVAAQLLNARVKLG
                     CASVSSLKPMILLRLLLNIKEIRFGFETDCK"
                     /name="hypothetical protein"
     gene            1705..1968
                     /locus_tag="IEE96_RS27475"
                     /old_locus_tag="IEE96_27395"
     CDS             1705..1968
                     /locus_tag="IEE96_RS27475"
                     /old_locus_tag="IEE96_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_097680169.1"
                     /translation="MKKICTLLMSIFILSACGEDTKSSDWWLNHPKEATEKYKECKKSG
                     EDSVNCQNVKKVAGIIGRTYGPMLEILKAESAEYDKQNGLNR"
                     /name="EexN family lipoprotein"
     gene            1973..2971
                     /locus_tag="IEE96_RS27480"
                     /old_locus_tag="IEE96_27400"
     CDS             1973..2971
                     /locus_tag="IEE96_RS27480"
                     /old_locus_tag="IEE96_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_052978250.1"
                     /translation="MSGFFEKFNSEVVSKIEEMTKPLQSQLGQDMLSLLSAGISVYILW
                     VGYQILAGKRQTPLQDLVWDLSKFAIILTFVSNADGYLTAAMDAIKSMKDGFLGKPVWA
                     TLDTMWSTTQLLADKVYAMDQSKYVSVEGGIGSMLVWGGSILIMSVAAVIYMLADVTMQ
                     LAILVAPIFIGCYMFGFTRVMFNNWLGILFSSVFTVIFAGVLVKIGTEFQTDMLSQISR
                     DANSTNILTMGAMAFVIGVLIAVFVWKSSSFAQQLAGAGVEGTLQGMALLGIGVAGMAV
                     EKTRNQMKRGTRTAAEWAGEKGEQKRASIGALGMEARRRASLEKARARNSA"
                     /name="type IV secretion system protein"
     gene            2968..3681
                     /locus_tag="IEE96_RS27485"
                     /old_locus_tag="IEE96_27405"
     CDS             2968..3681
                     /locus_tag="IEE96_RS27485"
                     /old_locus_tag="IEE96_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001590713.1"
                     /translation="MNKKLIFSVLLCLFSFGSVAGIPVAIDASPEWAVEATRWTERLKQ
                     WSETARHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELANIYKQGNSYISDFTSN
                     PEGALSSQAKNLFSKYGAFNMCSTGIESSDNLCKARIVSTAASIEQGNEINKQLSSAMS
                     QIQSLSNRIESSKDMKESQDLANALQAQSLKIQAIKMQYDVWDSKNRADAEMLKEQKYA
                     EYRLRQKNAKIPSFE"
                     /name="type IV secretion system protein"
     Typing          1974..2776
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="89.552"
                     /coverage="91.0"
                     /evalue="1.41e-160"
ORIGIN
        1 ggagaacccc agacccccat cgctgtaaca tcgacggtct acgaccacct accaaccgtg
       61 tggctacctg ttccgtgcta atgggctttg cagggggctg gcgcggttcc tggcgacctg
      121 aaagcccctg gttaggggta tcggcgcgcc tgacctcctg aaaccctctg taagcatttt
      181 tcgcgccttc catgccctga cgggcatcta aagcccgttt cgtgcgtctg agcgcgtttt
      241 aagagctaag acgactaaaa cattaccaac acctgaaaat cgcgttctgg tgctctggat
      301 ggcccacaga acgcattttc gaacagaaca gcaacggcat ttctgacaac atgcgagagg
      361 tgatcccgct cgccgcagcc ccagcgaccg aacgtagtga gcgagtgggc gaggaagcgg
      421 aagagaattg atagccataa ttgagcacct acgctcctta gttaagaact aagaagggaa
      481 attctcggaa cccgcatgaa tcctagaaag tggtgatcag gatctgtacc ttgtaagctc
      541 agatccggta ccttgcaggc tcaaaaattg cttgattttg agcttatgag gatttattgt
      601 gaatctccaa agcccaaaaa aggatgcaat aatgagctcg ctaagcttac agagatacga
      661 aacaaacccg tttttagagc ttatgacgat cccgatgaaa gggaaacgga tacaagtatc
      721 tcgtcttggg cgggatgaca atgttttggt taatcaaaac acgggagaaa agtttggaac
      781 acatgtaacc acttttaagc gtgtagatag tgagcaattt gttaagttgt ttactgcgaa
      841 tattgggtta acgtttgatt tatcatcggc aggaattaaa gcttttaatg ttcttttatg
      901 ggctgttcaa aaacaagcct ttgcaaagga tcaggttctt ttggattcaa gggctcttga
      961 tgacttttta aaaagctatg atagcttaaa acttagttat gctacgttaa agagaggaat
     1021 taacgagtta gaaaaagctc agattatagc aaagacaatg cgtaaaggat tttattttat
     1081 aaaccccaat tttgtattta atggagatcg aatagcattt acaaccctaa ttgagaggga
     1141 ttgttaatcg atagaacaat acaatgcagg atagacaatc cgatgtcaaa tgttaacatt
     1201 tgaatcagat tgtccaaaaa cgatgttttt gcctgtgcct gccgcaggct actagaagta
     1261 gtcgcaggcg aacaaaagtg gtgttttgtt cgcctgctaa tcacaacaac ctggctagag
     1321 gtttttatga ctaaacatga tgctatacgc atttctcaac ttcatcaaat tttttacgat
     1381 gatgatggat taattgattc agaaaagaac gtgactattt tatacagtat aggatttaca
     1441 gttgatgaaa tagctcattt tagaaattca acaccaaaca cagttgcggc tcagctatta
     1501 aatgcaaggg ttaaattagg ttgcgcctcg gtttcatctt taaagccaat gatcttatta
     1561 agattgcttt tgaacataaa agagattcgt tttggattcg aaactgattg caaataatat
     1621 agttcaatag gtatttttgc tcttgactag aattacaatt gctgattgta tcagcaattg
     1681 taatgtccat acgaggaatc aaagatgaaa aaaatttgta cgctcttaat gtctattttc
     1741 attctttccg catgtggtga agatacaaaa tcatcagatt ggtggcttaa tcatcccaaa
     1801 gaagcgacgg agaaatataa ggagtgtaaa aagtcagggg aagattctgt taattgtcaa
     1861 aatgttaaaa aagttgctgg aattattggt agaacctatg gccctatgct tgaaatactt
     1921 aaggctgagt cagctgaata tgataagcaa aatggattga acaggtgata acatgtctgg
     1981 tttctttgaa aagtttaata gcgaagttgt tagtaaaatc gaagagatga ccaaaccact
     2041 tcaaagtcaa ttaggtcagg atatgttatc tcttctaagt gcaggtatta gcgtttatat
     2101 cctctgggtt ggctatcaaa tattggcagg gaaacgccag accccattac aagatctcgt
     2161 ctgggatcta tcaaaatttg ccattatttt aacattcgta agcaatgctg atggctatct
     2221 gacggcagca atggatgcta ttaaatctat gaaggatggg ttccttggta aacctgtatg
     2281 ggcaacgcta gataccatgt ggagtacaac tcagcttttg gctgacaaag tttatgctat
     2341 ggatcaatcc aaatatgtgt cagttgaagg tggaatcggg tcaatgttgg tttggggagg
     2401 aagtatcctg attatgtctg tagcggctgt tatttacatg cttgccgatg taacaatgca
     2461 gttagctatt cttgtagctc cgatatttat cggttgttat atgtttggat tcactagagt
     2521 aatgtttaat aactggcttg gtatattatt ttctagtgta tttactgtta tttttgctgg
     2581 agttctcgtc aaaattggta ccgagtttca aactgatatg cttagtcaga tatcgagaga
     2641 tgctaactca acaaatattt taacaatggg cgcaatggca tttgtaattg gagttctgat
     2701 tgctgtcttt gtctggaaat catcgagttt cgcgcagcag cttgccggtg caggtgttga
     2761 aggtactctt cagggaatgg ctcttttagg gattggggtt gctggaatgg ccgtagagaa
     2821 aacgagaaac cagatgaaac gtggaacgcg aacagccgcc gaatgggcag gtgaaaaagg
     2881 ggagcagaaa cgggcttcaa taggcgcatt gggaatggaa gcaagaagac gagcatccct
     2941 ggaaaaagca cgtgcgagga attcagcatg aacaaaaaac taattttttc agtacttttg
     3001 tgcttatttt catttggttc tgtcgcaggt attccggttg ctattgatgc cagtccggaa
     3061 tgggctgttg aagccacaag atggacagaa cgactcaaac aatggtctga aacagcgcgg
     3121 cattatcaaa gtcagattca ggcatataaa gaccagttag ccacggcaac aggtataagg
     3181 aatattgcgg cattcacaaa tgaactaagt aacctacaat cagagctggc aaacatttat
     3241 aagcagggga acagttatat ttctgatttt accagtaatc ctgaaggtgc attaagtagt
     3301 caggcgaaaa atctgttcag caagtatggt gcttttaata tgtgttctac aggcattgaa
     3361 agttctgata acctttgtaa ggccagaata gtttcaacgg ccgctagcat tgaacaggga
     3421 aatgagataa ataagcagct atcaagtgca atgtctcaga ttcagagttt aagtaaccga
     3481 attgagtctt caaaggatat gaaagaatcc caggatttgg ctaacgcatt gcaggcgcag
     3541 tcgttaaaaa tacaggcaat taaaatgcag tatgatgtat gggatagtaa aaacagggct
     3601 gatgcggaaa tgttgaaaga gcaaaagtat gctgagtatc gcctgcgtca gaaaaatgca
     3661 aagataccga gtttcgaatg atatgataaa gctaaataag ctaaaagaat gggtagataa
     3721 tcattggtct agattgaata gtaccggttc tacaccgccg ccactgttaa tctcgctacc
     3781 aatcatagtt attgttttaa gtgtggagct tacgttaaag ctggttaatg ctttaatgta
     3841 ttatggtaca ttgcaattta tcactgataa aattgaaaag aggtttgttc ccagcggtgg
     3901 tataaaagaa agactccttt ttacgttaaa gctgttttgt attttacttc tgttattaat
     3961 agtaatgcga ttttcaatga agtaaaaaat aaaggtcatc gaatagatgg ccttttctat
     4021 tctaaattct aaatatagag cctgcggcag gcacaggcaa aaacatcgtt tttggacaat
     4081 catggataaa atgttaacat tttgaccatg attgtctatc ctgcattgct cttttgccga
     4141 cctcaaaacc cctagcgtgt ttttgtttcc cgatctctag aaccaccgag ccgccacggt
     4201 tccggcagcg ccttgagcgc aaccggaaac cgtctgg
//
