LOCUS       NZ_CP069209             4715 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Klebsiella pneumoniae strain SP968 plasmid pSP968_5.
ACCESSION   NZ_CP069209
VERSION     NZ_CP069209.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16178551
            Assembly: GCF_022701055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Carbapenem resistant K. pneumoniae VSP968 hybrid genome assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. August 2020; unicycler v. August 2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022701055.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:49:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,519
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,278
            CDSs (with protein)               :: 5,278
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 50 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 16 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069209.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SP968"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pSP968_5"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College, Vellore"
     gene            complement(34..228)
                     /locus_tag="IEE96_RS27445"
                     /old_locus_tag="IEE96_27365"
     CDS             complement(34..228)
                     /locus_tag="IEE96_RS27445"
                     /old_locus_tag="IEE96_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1304..2260
                     /locus_tag="IEE96_RS27450"
                     /old_locus_tag="IEE96_27370"
     CDS             1304..2260
                     /locus_tag="IEE96_RS27450"
                     /old_locus_tag="IEE96_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000280763.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            2244..3110
                     /locus_tag="IEE96_RS27455"
                     /old_locus_tag="IEE96_27375"
     CDS             2244..3110
                     /locus_tag="IEE96_RS27455"
                     /old_locus_tag="IEE96_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_000409995.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            3092..3760
                     /locus_tag="IEE96_RS27460"
                     /old_locus_tag="IEE96_27380"
     CDS             3092..3760
                     /locus_tag="IEE96_RS27460"
                     /old_locus_tag="IEE96_27380"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_223195384.1"
                     /translation="MGYIQMIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCS
                     EWNMKILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYN
                     KSGVLLTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFD
                     LNDENKEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS
                     "
                     /name="PP2C family serine/threonine-protein phosphatase"
     gene            complement(3982..4095)
                     /locus_tag="IEE96_RS27645"
     CDS             complement(3982..4095)
                     /locus_tag="IEE96_RS27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427893.1"
                     /translation="MHEPPVQPDCCALSGNYRLESNPVRHALTPLAAATGN"
                     /name="hypothetical protein"
     Typing          265..363
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          488..743
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          4592..4676
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(4024..4581)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.821"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcggacaagc cgtgagcgcc tccgtcagct tcgttatgtt cctgtttcac tttcggtctc
       61 aaagcgaacc tggatgctgt tctggagttc ttccgcgagt tcgtgcagtc gttcgcacat
      121 ggccgcctgt tcgtcggcat ccagagcatc cagtttttca agcagtgtca ggctctggct
      181 tttgataaat cgcgccatat tcagcgcagt ttgttgctgt ttgttcacct tccgttgttc
      241 tccgtcagta ccgggtgtcg ggtggaccct gaccaggtgg taattgtaag acgttgcggg
      301 cgacggtatt acaattgcac atcctgtcct gtttttttag gacgtatcat ggcacagcat
      361 atcaactgat aaataatccc tgtgcagtgc agccgctgtt gcataaaatt tcgctctgcg
      421 tcgagtgttt cttaatcaat aattccgctg atactccgga aaaacgcgag tctgttctca
      481 tgcctgaaat gcccacaccc cacgcaaaaa caattttttg ctgatttttc tttataaata
      541 gagagttatg acaaattagt tcttcttgct ctctttgtga tatttaaaaa agcggtgtcg
      601 gcgcggtgtt gtagctgcgc caacaccgct ttttaggggt ggtactgact attttcataa
      661 aaaacatcat tttatattag gggtgctgct agcggcgcgg tgtgtttttt tacaggacgc
      721 ccctgggggc gctgctaggg gtgtctgttc agatatgtag caatgagtca gggattcacg
      781 aactgaaaat gtccgaaaat gtgtctcatg ggcactgtat gcgcccaaac cgatcgtcat
      841 tcactttcat gcatagctat gcagtgagct gaaagcgatc ctgacgcatt tttccggttt
      901 accccgggga gaacatctct ttttgcggtg ttcgcggcag aatcgcgttc agcgcgtttt
      961 agcggtgcgc gtaatgcgac gttatggtaa atgacgctga aaaatgatct tttacgctca
     1021 tcgtttatga gcaacagatg tcctaatcat ggatttgaac tataaccggc gctgaattga
     1081 tcgcatgagg tgttatgaaa acggtgatat aagtaaactt tttcttgact tcctacttat
     1141 tatcctataa aataaatcct taggatttat tttataggat atcaaaaata taaaaatggc
     1201 aaatgcttgt ttgttattat ttttttcttg acaatcaatc tctaatagaa tatacgtcct
     1261 taggacgtat attctattag agactaaacc gaaaaggttg aatatgtatc ttatcgaaga
     1321 agcgctaacg tcttggttgt tgaatgaata taaaaaacca gtcataacca aagaagaaat
     1381 ctctttgcag atttaccttt tttatttaaa aaaagaatat aatggtgaga ggatatatca
     1441 aattagaaag aacaaagcag gacctaatga atttgaaaaa aatatattaa aattattaaa
     1501 gtcaggggtt ttatcaagag ttgaaagtaa cgataagcat catgcttacc cccatttaat
     1561 tggcggtttc tatgaggtct tttttgttaa aggtaacgat ggttctccgg aaaatgatat
     1621 agtgtgctct acgtatccat atggatactt atcctattta tccgctatgg attggcataa
     1681 catcacagat aaaataccca agataatcca ctttacgaca tgtcgttccg aacagtggaa
     1741 gaataagttc tatcatgatt ttcaaaagga taatcctgat atagacgaag ccaaacaatt
     1801 catgcctaga tttccaagaa attctgaggt caatggaata aaaattatcg ttacacaaga
     1861 aacaaattat gttgagccaa tgaaggctcg taattcccct gtcagggtat catctatcgg
     1921 taagaccttt atggatatga ccaggcggcc tgattactgt ggtggtgagg agcatgtatt
     1981 ggaatcattt attgagcacg gtaaaaaata tgcacctctg attataaagg aagtcgataa
     2041 gcttggccgt catattgaca aggtaagggt tggattcatt ttggatagaa ttattggggt
     2101 taataataaa aaaataagcg aatggaaaca agaggcgggt aaatacagag gtagttcgaa
     2161 agtgctatct ccaaagagtc cattctcacc ttactttgat gaagaatggt ttatttctat
     2221 taatgttgat atggttaagt cttatggaaa aagaaattaa taattggatc gaagagtctg
     2281 gtgggaagta caggttgatg cgccaggctg tacatataat acttaaatct atttctatag
     2341 aggagagttt gaatgaaatg atggttatga agggagggac tcttcttggt gttaagcatg
     2401 gtagtgatag atacacaaag gatattgatt tttctaccga aaaaaaactt tctgaaattg
     2461 acttggggga ttttttcaat aggtttaatg actcaatgga tgttgctgag gctgaattgg
     2521 gatatggtat taaatgtaaa gttcaatctc ataaaattca acctaatgca caaggaacat
     2581 ttccaactgt acagataaat atagcctttt gcgatcgttc taatgcgtct gcaatgaaaa
     2641 ggcttgaatc taatcaatca ccttcagtga taaatatcga ttttagcttt aacgaaaaaa
     2701 cgtatgattt tgacttttta tcattagatg gtgatgatga caatgatagc gtaaagacct
     2761 acagcttaac tgatttgatg gctgaaaaat atcgatccgt tttgcaacaa aaagttagag
     2821 agagaaatcg agagcaagat atatatgata tcaattatct tttaggtaaa tatgagtttt
     2881 ctcggcaaga taagtataaa atactggagt ctcttttaaa aaagagtgaa ggtaaacaat
     2941 tggataatct cttaaacgtt aaaggtataa gggatgtaga aattatcgag cgttccagtc
     3001 agcgatatca ggatttatca tccaccgtta aatcattacc tctttttgaa gatgcttatg
     3061 aaaaagttgc ctgtttctat gaaagtcttc cgtgggatat attcaaatga tagcgttcag
     3121 ttttagtgat attggtaaca gatgtgaaaa cttagactta ttatctacga taaattgtca
     3181 aaatgatgtg tttggaatgg ttattatgga tgggtattcc tgctctgagt ggaatatgaa
     3241 aattctctct ggtaggttag ataagtgcgt gttttctggt gatctgaata aagatattat
     3301 tgaaccatgc aagtcagttc ctgttaaggc tgcagtatgt ttccttttta aaaataatac
     3361 gcacctccat atatataacg caggagattg ccgtgtatat aacaaaagtg gagtccttct
     3421 aacagaggat aactcggttg cctggaaaag attaaaggaa aatggaatcg ataataaaaa
     3481 aatacctcac ctcgtttgtt attgcccaga cagaaattat ctaacatcat ttgttgattt
     3541 ctgctctgat gagataattt tcaatcattc agtttttgat ttaaatgatg aaaacaaaga
     3601 ggttatttta tgtactgatg ggttttggga gcattttaat gatggtactg ttattgacct
     3661 catgaaagat aacccagaat ctaacttggc taaatttact aaaaatatcc cgggtaacgc
     3721 agagaatcat acggtttgct acgttagatt cccttcttga tttcgtgttt tttgaagaag
     3781 ctattgagat cttcgtgtca tatagataat ctcttttctg taaaagaaaa aaaccgcctt
     3841 tgcggggcgg ttttctctta ggttcaaaga gtttgcggct cgaagaacca aggtaactgg
     3901 cttggaggag cgcagtcacc aaaacctgtt ctttcagttt agccttaact ggcgcatgac
     3961 ttcaagacta actcctctaa atcagttacc agtggctgct gccagtggcg ttaaggcgtg
     4021 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcagt cgggctgaac
     4081 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     4141 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4201 ggtaagcggc agggtcggaa caggagagcg cacgagggag ccaccagggg gaaacgtctg
     4261 gtatctttaa gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tctgtgatgc
     4321 tcgtcagggg ggcggagcct atggaaaaac gcctgcggcg cggccttctt cctgttcttg
     4381 gtcttttgct cacatgttct ttccggcctt atcccctgat tctgtggata accgtgctgc
     4441 cgtttttgtg taagtcagta ccgctcgccg cagccgaacg accgagcgta gcgagtcagt
     4501 gagcgaggaa gcggaaaagc gcctggacgc gcattttctc cttacgcatc tgtgcggaat
     4561 ttcacacccg gcatggggca cttttcatac aattcgcact gatgccgcat agttaagcca
     4621 gtatacactc cgctatcgct acgtgactgg gtcagggctt cgccccgaca cccgctgacg
     4681 cgccctggcg ggcttgtctg ctgccgaaaa cgctc
//
