LOCUS       NZ_CP069208             5409 bp    DNA     linear   CON 27-MAY-2024
DEFINITION  Klebsiella pneumoniae strain SP968 plasmid pSP968_4.
ACCESSION   NZ_CP069208
VERSION     NZ_CP069208.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16178551
            Assembly: GCF_022701055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Carbapenem resistant K. pneumoniae VSP968 hybrid genome assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. August 2020; unicycler v. August 2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022701055.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:49:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,519
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,278
            CDSs (with protein)               :: 5,278
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 50 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 16 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069208.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SP968"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pSP968_4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College, Vellore"
     gene            complement(1125..1406)
                     /locus_tag="IEE96_RS27415"
                     /old_locus_tag="IEE96_27335"
     CDS             complement(1125..1406)
                     /locus_tag="IEE96_RS27415"
                     /old_locus_tag="IEE96_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000594459.1"
                     /translation="MIKNIKHKGLRSFAENGNTAGIQPHLAKKLRMILTTLSTATCLQD
                     IVDVRALGCHPLKGERKGEFSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1744..2037
                     /locus_tag="IEE96_RS27420"
                     /old_locus_tag="IEE96_27340"
     CDS             1744..2037
                     /locus_tag="IEE96_RS27420"
                     /old_locus_tag="IEE96_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_000609279.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLEVSQSALSRLLN
                     GKTDLSYEMALRLSKVIGRSAESWVNMQAQYSLSKSRKTLSETLEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            complement(3569..3784)
                     /locus_tag="IEE96_RS27425"
                     /old_locus_tag="IEE96_27345"
     CDS             complement(3569..3784)
                     /locus_tag="IEE96_RS27425"
                     /old_locus_tag="IEE96_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3791..4282)
                     /locus_tag="IEE96_RS27430"
                     /old_locus_tag="IEE96_27350"
     CDS             complement(3791..4282)
                     /locus_tag="IEE96_RS27430"
                     /old_locus_tag="IEE96_27350"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_044715458.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHERSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<4317..5021)
                     /locus_tag="IEE96_RS27640"
                     /old_locus_tag="IEE96_27355"
                     /pseudo=""
     CDS             complement(<4317..5021)
                     /locus_tag="IEE96_RS27640"
                     /old_locus_tag="IEE96_27355"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4960..5283)
                     /locus_tag="IEE96_RS27440"
                     /old_locus_tag="IEE96_27360"
     CDS             complement(4960..5283)
                     /locus_tag="IEE96_RS27440"
                     /old_locus_tag="IEE96_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2..160)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.625"
                     /coverage="92.0"
                     /evalue="2.62e-66"
     Typing          85..671
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.881"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(3478..4970)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccctgaacc agtcacgtag cgctagcgga gtgtatactg gcttactatg ttggcacaga
       61 tgcggatatc agtgaagtgc ttcatgtggc aggagaaaaa tgcagcagcg gagcgtaagt
      121 ggaatatgtg atacagaata tattccgctt cctcgctcac tgactcgctg cgctcggtcg
      181 ttcggctgcg gcgagcggga atggcttacg gacggggcgg aaatttcctg gaggatgcca
      241 ggaagaagct taacagggaa gcgagaaggc cacggcaaag ccgtttttcc ataggctccg
      301 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
      361 actataaaga taccaggcgt ttccccctgg tggctccctc gtgcgctctc ctgttcctgc
      421 ctttcggttt accggtgtca ctccgctgtt atggccgcgt ttatctcatt ccacgcctga
      481 cactcagttc cgggaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      541 agtccgaccg cagcgtctta tccggtaact atcgacttga gtccaacccg gaaagacaca
      601 acaaaacgcc actggctgcg gccattggta agagggtgta caagagatta ctgatgcaga
      661 gttcttgaag tcagagccat actgaggcta aactggaagg acagaatttg gtttcagcgg
      721 tcttgtacac cctgttacca cggttcagaa gttccccaac ttactgaacc ctcgaaaaaa
      781 ccgcctcccc aggcggtttt ttcgtttcga acataagata cgctttgtcg atttaatcag
      841 tcaaggaaga accctgaaag cctcaatcgc ttacagaatt attcttgtta gagacatgtt
      901 tctgcaccgt atcaacacca gtacacaaaa cagctgcaaa caggaaccat ccccacccat
      961 ctaggccacg aacagcaaga accccagcaa tgactacaca aagcaaaggg aacaacaaaa
     1021 tcataaaggc tctcataaat cctccagagg tacttatttt tgaataaagt ccctaatcca
     1081 tccatattgc cactaggatg gattagtaac ttacttattg attactaatg gtaatcttca
     1141 agatcaacgt ctaagacgtt tggttcttcc accttgaaaa ccaccctcca gtttccgtta
     1201 acctctacgg aaaactcacc tttacgttca ccttttaggg ggtgacaccc cagcgccctt
     1261 acatccacaa tatcttgaag acatgttgcg gtgcttagcg tagtcaatat catgcgaagt
     1321 tttttcgcta agtgaggctg aattccggcg gtgttaccgt tttcagcaaa actccgaagc
     1381 cctttatgct ttatgttttt tatcatcaat catctccata aaattttcgt cgatatctat
     1441 ctctataaac agaaaacaat ttgacagcgt ccatttgaac cccttgcatt attaaaataa
     1501 ttgacgtaac tttgtctctg aggtcgatta cctcgtttga cttccaagag cgctaaccgt
     1561 acaatatggc gccttgaaaa agtcggtaaa gacaccatat cagcggtagc tttctgctcc
     1621 accgagcatc ctaaagctta ctgctttttt tgtcaataac agacacttgc gtattgtgca
     1681 atgcgcaacg cgaaagaaac aaaatgtttt gtgtaatcaa atacaaaacc aatagaggaa
     1741 ggcatgataa atctttacga tcctgtacac cccggtgaat tcatcaggga aatgttcatg
     1801 gaaccgttcg aaatacctgc cgcagagtta gctgataagc ttgaagtatc acaatctgca
     1861 ttaagtagat tgctcaatgg aaaaactgat cttagttatg agatggctct tcgactatct
     1921 aaagttatag gccgctctgc agagtcgtgg gttaatatgc aggctcagta cagcctctcg
     1981 aaaagtcgta agactttatc cgagacgcta gaacaccttc atccgatgtt tccataagta
     2041 aaagcccgct cgtgcgggct ttttgccgta aaacccccgc atacctacct ttagtttgat
     2101 ttttagttca gttcttagat cttgaaatga ttgttgtttg aacaccaata agatgcccat
     2161 acaaggctct aaaaaggaga acaaaatggc aatttatgta ggaccacatg gcaaaatagg
     2221 tcgtgatggt ggctcattcc aagaatgtga catcaacaca aacactcata ctgttacccg
     2281 caaatatgga cgatactgca ccattcgcga taatgagttc gcaccaccaa cgagcgcacc
     2341 tggcaggtac tggctacaga tctaccgaac cccagacaac actgagtaac agaagcgcca
     2401 gtaacgacaa agcccgcata agcgggcttt gtctcttaca aaattcctcg tcgtcgcaat
     2461 tcagcttcta tcgcctcaca tgcggcatcc acaatctccg tgttgtagta cctggacatg
     2521 ccactgctaa tgatatcgtc cattagggca cggttatgag ctaaggtacg tcgtaagtat
     2581 tcaggggtat gttgctccat cggattctcc ttttcatgac tgagcattat tgcttacctg
     2641 tatttatccc cgtcagtgct ggtcacgttg aaaagcccta aaaaaccact tttttagctg
     2701 ccttaatcat tgaaaaatgg cacttaaatc cacatctatc gcgctgggga aatcgcccgc
     2761 acacagccac tgataaaata aaagtgagat aaaatggatc caatggataa gatttgcgat
     2821 gtttgtgccc tccctgccag cgcctgtacc tgtggcttct gcgatgactg caacgagaag
     2881 ctggcagact gcgtctgtga gcgctgcagc gattgtggaa atttagatga gcactgtagc
     2941 tgctcacgtt gcgacgagtg cggtgaaaaa ctggatgagt gcatatgtga ggagtgcgaa
     3001 gaatgtctcc gcagtccaca tgattgtatc tgcgaaaact gacctaaggc cgaattctct
     3061 ttcaagagcg accgggacgg tcgctcttat ttaccataac atccgttgcc cgcaccgctg
     3121 aaacggggtt ggctgcgttt ccggtgccag acgcgagaaa gtggcgacag acataacgaa
     3181 aaaacaccag aaacgcagcc gctgaatatt acactggtgc atagtcatgc agccagatgc
     3241 atggatgtta aagtcacact aaaatccgcg ccaatgcctt aaaaaccgac aaatgcttct
     3301 ccagccagtc ccacagctcc gccgccagat acaggcagtt aagcaatgat tcaataagat
     3361 ttaacagctt catttcccgc tcctgaataa gtcgtgacct ttttgatcac gaacctattt
     3421 agcgggagaa ataagagccc ctcttcgtgc gggttgtcag ctgcctggat ggtttaaagt
     3481 gtcggcccgc ggtaggtttt ctgcggctgg cgctccagct ccagtgtttt ctgatactca
     3541 ttctccctct gccggaactc ggtggcattc agttcccgga caagcggcgc agctgctcgc
     3601 ttaaactcag cacctgcgtg ctcaagtccg acacgcgctc gttcagcacc gcgttctccc
     3661 gttgcataag cccacacatg ctccgccatt ccgcgaaggc gctctcccat tcgtccagcc
     3721 tttgcgagta gtcctgctgt agctgctcta atgcgctcag caactgcttt tccagctctg
     3781 tcattttccg ctactcctgt ttcaggcgga tccatgtcgt cccgtcgtag gggatggctt
     3841 cggtttgcat ccccggcggc aggataagaa acctccggcc gtcgctgctc tcctgatagc
     3901 gcactcccca cgtccgggcg gtcattttcg ccagggtatc ttcctgctga cgaagatgcg
     3961 tgtaattgtc ggttatctgc tgcccctgcc accacagaat gctcccgctc gtgccgatca
     4021 gcagcgctga caccatcagg atggtgagcc atgtccgccc ggcagtccgc agtacgctcc
     4081 gccggttttt ctccatcgcc tcgctcatac tctgctcgtg ggcgaggatg gcgtcgctga
     4141 ttctcctcgc gcttgcgctc agttccgctc tgacagaccg ctcgtgctcg ctgaatgcgg
     4201 ctttcagcat ctcgccggta ctctgctgct gcgctttcga ttgctgctct aagtccttcg
     4261 ccagcgttaa aagactgctc atagatggct cccttcagtc ggatgtttcg ccccccgtcc
     4321 gggtcggcaa tgctgatact gcttttcgtg gtgcgcacca cctcaaaacc tgcaccttca
     4381 agagtgtcag tgacatcctg acggcttttc agctccccgg acgaagcaag ggtcagcagt
     4441 ccccgggtaa tggcttctgc ggcctcctgc ttcgttttcg gcagcccgga cggcgtgacc
     4501 agcgcccggc ggttttccgg cgcgttcggg tcgtgcagcc ccagtctgcc gttcacgata
     4561 gtctgccagg catcgatgcg cggccggtcg gcgcggtcgt agtaaggctg gagccgcctg
     4621 ccggtcagca gctcggtatt ggggatcaga aagttcagct ccagacgccc cttgtccgcg
     4681 tgctccaccc acagaatgct gtactgatct ttatcgagtc cgggcatcag aacccgctcg
     4741 aagctggcca tcagtttttc ccgctggccg ggaggcagat cggcctccgc aaacgacaga
     4801 acgccggagg tgtatttctt aacgtaaggc gacgcgtcaa tcagctcccg gacttcttcc
     4861 ggcttcccct gcaggacggt ggcgccttca cgctgccggt ctttacccag cagatagtcc
     4921 accggcccgg cgccaccgcc gcggccgcgc ggatgaaatt taacgatcat cgtccgttcc
     4981 tttctcaaga acggcgtgcc gcagccgctc aaggccggca tcgatggcca tcagcgccgc
     5041 cacaatctgt accctgtcat ggccggtgcc gccgccggcg ttaacacggc gggctatctg
     5101 gttcaggtta ttgcccatgc cggcaagctg gcgcagcagc gccggcgaga gtgaggggag
     5161 ctttccggaa cgtgccggct tttcgtccag gcagatctgc cgcatccacg ccgccagctg
     5221 cctgccgtca cagcgctcga gcagccgccg gtgctcgtcc tccgtcaccc acatggtgag
     5281 catcttgctc cgtttctctg acaaaccggc ctcctgataa cgccgggacg ggcgggaaag
     5341 cctttcccgg tcggcgcggg gttggacaag ccgcaggcgc gtcaggcttt tggcgggttt
     5401 cggggcgca
//
