LOCUS       NZ_CP069207             9302 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Klebsiella pneumoniae strain SP968 plasmid pColKP3, complete
            sequence.
ACCESSION   NZ_CP069207
VERSION     NZ_CP069207.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16178551
            Assembly: GCF_022701055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Carbapenem resistant K. pneumoniae VSP968 hybrid genome assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9302)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K., Vasikaran,P. and
            Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. August 2020; unicycler v. August 2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 68.2x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022701055.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 04:49:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,519
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,278
            CDSs (with protein)               :: 5,278
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 50 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 16 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069207.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SP968"
                     /isolation_source="endotracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColKP3"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College, Vellore"
     gene            1..948
                     /locus_tag="IEE96_RS27375"
                     /old_locus_tag="IEE96_27285"
     CDS             1..948
                     /locus_tag="IEE96_RS27375"
                     /old_locus_tag="IEE96_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            1356..1748
                     /locus_tag="IEE96_RS27380"
                     /old_locus_tag="IEE96_27290"
     CDS             1356..1748
                     /locus_tag="IEE96_RS27380"
                     /old_locus_tag="IEE96_27290"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            1730..2068
                     /locus_tag="IEE96_RS27385"
                     /old_locus_tag="IEE96_27295"
     CDS             1730..2068
                     /locus_tag="IEE96_RS27385"
                     /old_locus_tag="IEE96_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2049..3503
                     /locus_tag="IEE96_RS27390"
     CDS             2049..3503
                     /locus_tag="IEE96_RS27390"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3622..4227)
                     /locus_tag="IEE96_RS27395"
                     /old_locus_tag="IEE96_27315"
     CDS             complement(3622..4227)
                     /locus_tag="IEE96_RS27395"
                     /old_locus_tag="IEE96_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509965.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGCAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            4322..7219
                     /locus_tag="IEE96_RS27400"
                     /old_locus_tag="IEE96_27320"
     CDS             4322..7219
                     /locus_tag="IEE96_RS27400"
                     /old_locus_tag="IEE96_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            7571..8368
                     /locus_tag="IEE96_RS27405"
                     /old_locus_tag="IEE96_27325"
     CDS             7571..8368
                     /locus_tag="IEE96_RS27405"
                     /old_locus_tag="IEE96_27325"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /protein_id="WP_015060052.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTRIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
     gene            complement(8775..>8927)
                     /locus_tag="IEE96_RS27410"
                     /old_locus_tag="IEE96_27330"
                     /pseudo=""
     CDS             complement(8775..>8927)
                     /locus_tag="IEE96_RS27410"
                     /old_locus_tag="IEE96_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     ARG             7572..8365
                     /name="blaOXA-181"
                     /gene="blaOXA-181"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          14..935
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2050..3500
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgagaaagt gcgcacagct tgagctgttt cgggagcgcc tgccccggaa accctactat
       61 tccgacgagc ttacaacggg cctacggatt gcggatgttg cccgcgccct tggtgcgcgt
      121 tacatccagc cgaacgggcc aacgcaccgc cactggatcg tttttgacgt agaccatgca
      181 gccgctacgt tatcttggga tgacgtaggc gctccagccc ctaacattac cgtcacgaac
      241 aaggccaacg gccacgcgca tctgatctac ggactggata cgccgatccg cactgccccg
      301 gatggaaatg cagcccctct gcggtatgcc gccgccatcg aggccgcgct ccgggagaaa
      361 ttaggggcgg atatgggcta ctccgggctg atctgcaaga accccctgca tgagcactgg
      421 ctggtgcagg tatgggaacc ccggctctac gatttagcct ggctatccga ttacctcgat
      481 ctgagctcct acaatggccg caaatcgctt cctgagtacg ggcttgggcg aaactgtacc
      541 ctgttcgaga aaacgcgcct gtgggcctat aaagcgatcc ggcagggctg gccggattac
      601 cccgaatggt tggccgcttg tgttgaccgg gcatcaggat ataacgccca gttcgagcag
      661 ccgctccccg caaacgaagt ccggcacacg gccaagtcca ttgccaaatg gacgcaccag
      721 cacctaagcc ccgcagggtt cagagaggag caggcccgga gaggtgcgaa gggcggcaag
      781 gtatccaagg gagggggaag gccgagcaat agcggtaagg acaagtccga tctattgccg
      841 gaggttctgc ggcttaaggc tcaggggtac accaaccgcg atatagcgga cgatcttcaa
      901 atctccccca gtacggtgag cgtttaccta aagcgtgatc gtccgtgatc gaaacaagcc
      961 ttatcagata atagccccct ttaggggggt tcctgcgctc ttgtctttta gccttaaaaa
     1021 atccttttag cgcggatctt tggcgaggtg gacaactgcg cctgtccgtc catcgccccc
     1081 ctgttttttc ggggggtgtg ggcggcagca gttatccacg gagccgctct ttcccttgat
     1141 cttgctctag ggcttgcagt atcccgccca tggttgaata atgtactaag caaggctaca
     1201 cagaggcccc tctacctgct tcgcggtctc gggcctctgt ggccttgttc aggggtgcta
     1261 cgcccccccg aaaccccctg tggcccccgc tcgatccgcc ccttcgggac ggtctgcgcg
     1321 gtggcttctt ggctctggag aatggacgag gccctatgtc aacaacacag atagagaaag
     1381 attcgagaag gaaggcagtc aatggcgtta tttctagttt ccaactagag ggggaggcca
     1441 taaccgatga attcaaaatt ctacttgaag attatatcaa tggaaaaatt tccaccgatg
     1501 aggtgttagc tatggtaagt gacgaaccca caacaacgga atacgactac gaaatcaccc
     1561 cccgcactct cggtcttggc gggggctgga atctgcgttt gctggagaac ggcgaagagg
     1621 tcggcggggg tgttttcccc ttgccggagc actgcgattt tcgggatgaa aaggcgctcc
     1681 agacgctact cgatagcctc tatgaggacg ccttagcgga ggcctctgca tggctggcca
     1741 gccgttaaag cgcctccgct ctatcaagat ccgggtctca gatgcggagc ttgagcgcct
     1801 gcgggagctc tgccccaagg cccaactggc ggagtggatg cgggagcaat gtctcggtgt
     1861 tgcgcaaccc cagcgccgaa cgccggcgcc taccgtagat ccggcgctct tgcggcagtt
     1921 ggccggaatg ggcaacaacc tgaaccagat cgcccggcgc gttaattccg gggagtgggg
     1981 gccgttggat cggctgcgca tcatagcgga gctgtccgct ataggccgcg agctggagga
     2041 actgcaccat gatcatcaaa ttccataacc gtggggtagg ccggggctcc gggcctgttg
     2101 gctacttgtt gggccgtgac gggaaccggg aaggcgcaac gctcgaccgg ggcaaccctg
     2161 cggtgattga agccctgatt gattccagtc cctacgccaa aaaatacacc tctggcgttc
     2221 tgtcgtttgc cgaggctgat ttacccaggg cgaccaagga caagctgatg agcgactttg
     2281 aaaaggcgct cctgcccgga ctggagggca accagtacgc tgttttgtgg gtagagcacc
     2341 gcgacaaggg gcggctggag ctgaattttg tagtcccgaa catcgaattg cagagcggga
     2401 agcggctgca accctacttc cacaaggccg acaatccgcg cctcgatgcg tggcgaaccg
     2461 tggtaaacgg ggagctgaaa ttgcacgacc cggacgatcc gttaaaccgt caggccgtta
     2521 tcacgccaaa ggatttgccg agggggcaca aggaggccgc tcaggccatt acagacgggc
     2581 ttttacgcat ggccggagca ggggagatcc agagccgcga tgatgtgcgc aaagccttgc
     2641 agggtgcggg tttcagcgtg gtacgcgaaa ccaaaaacag cctgagcatt gccgacccgg
     2701 acggggggcg gaacatccga ttaaaggggg tgctgtatga gcaagatttt agacatggcg
     2761 aaggacttcg agcagagctc gaagcagcag gccaacgcta tcgagaagcg tctgaccgaa
     2821 gaattcacga ggcacgagaa agctatctcc ggggcgttga aatcaagcga ggagaaaatg
     2881 agcgccgcta ttgccgagca cagccagaaa atgggctggc tgttgctcaa aacgtggatc
     2941 tggatgctgg tcagcatcgt cctgttcttg agcgcaacga gcgggatcct ctggtatcag
     3001 ggggaattga tagcggagcg gcaagccact ctccaagcct tggcagagaa gggcggcaag
     3061 ctaaagctga cccagtgcgg gaagaaattg gagcaccttt gcgtgcagat ctccgacccc
     3121 aatccaaccg tttgggggaa ggatacctat tggatcgtgc cgaaggggta ctaaatgacg
     3181 ggattggagc gggaattgct ctgcgcgttc gaggctttgc agagcgcata cgagcagcag
     3241 cacaaggcat ggcaggaaag ctacgcgaac ttggcgagca tgtacgagag caccaaacaa
     3301 gaggccgaga tcagcagggc agcagcagag cgattgacga ggcaggtgaa cggcttaacg
     3361 cagcaagtgg acggcttgag caagcagctg gagggcttga ccgggctagt cagcaagttg
     3421 caaaagaaag ggaacacagg ctcctgaagg cactggatcg aggctgggag cgtggagaca
     3481 gatcgcgagg ctttgagcgt tgacggaaca aaacggccgt cagttataaa aaaaggaaag
     3541 tgctatcact ttccttttat cggcgggaat tatcgttgag ttcttctagg ggtcggttcc
     3601 ggctgagggc gaaatgacac cctaagcgtt agctctgtgt cgttgcacga tgtcagcgac
     3661 ggtattcttg ctgataccga gctcgcgtgc gatccagcga tagctgcgtc cctcggccct
     3721 catcgcaacc accttaggca aaagtcggtc tgattttggt cgcactccgg cctgacgacc
     3781 aagcctctta ccacgtgcct tcgcaacagc aaggcctgac ttgacccgct cgctgatgag
     3841 atcccgctca aactccgcaa tgccggaaag aaacgtcgcc agcattcgtc catacggcga
     3901 cgaaagatcg aacgccattc cattcatggc tatcacggaa accttccagt tctccagttc
     3961 acgtagcgta ttgagcagat cgagcgtcga gcgcccccac cgggaaagct cagtgaccag
     4021 gattgcatca atttgtctgg actgggcaag cgccaggact ttctttcgct cggcccggtc
     4081 gagtttagtt cctgaacctg tttccttaaa tattcccacc acgtcgtagc cggcacagcc
     4141 ggcgaaggct cgcagatcaa attcctggcg ttcacaagac tgatccgctg ttgaaacccg
     4201 gcagtaaatg gcggcacgat gtcccaattg aaccctcctg gatttttgta tcggaacgcc
     4261 ctgatttata tgggctggct gttgtccaaa acagactata cttcaaaagg gacgaatttg
     4321 tatgtcacga cgccatattt tcaccgaacg gcagcgagca gcgctgttcg atctgcccac
     4381 ggacgaactg tcgctactga agttctacac gctgggcgat gatgacctgg aaaacattag
     4441 gcagcgccgc agaccggaaa acaggattgg ctttgccctg caactttgtg ccttacgata
     4501 tccgggccgt gcactggctc ctggtgagat gatcccgcgt gaagtccttt ccttcgtcgg
     4561 tgctcagctt ggagttccgg ctgatgcgct tctcacttat gccacacggc gccaaacccg
     4621 tcagcagcac atggacacgc tgcgcgaaat ttacggctac aagaccttca cgggccgtgg
     4681 tgcccgtgat ctgcgggagt ggactttcgg ccaggccgaa gatgccagat caaacgagga
     4741 tcttgctcat cgttttattg tgcggtgtcg ggaaacttcc accattctgc ccgcagtatc
     4801 gacaatcgag cgcttgtgcg cggatgctct ggtcgccgct gagcggcgga ttgaaacgcg
     4861 gattgcggaa aatttaacag cggatgttcg cgatcacctg gacaaacttc tgagtgaaat
     4921 gctcgccggc aatatcagtc gtttcatctg gcttcgcaac ttcgaggttg gtaacaactc
     4981 ggctgctgct aaccgtttgc tcgacaggct cgaatttctg cgtaccctga atatcaatca
     5041 tagtgctttg gccagcatac ctgcccatcg cattgcccgg ctgcgtcggc agggtgaacg
     5101 ctacttcacc gacggtttgc gtgacatcac ttcggaccgc cgctgggcga tccttgccgt
     5161 ctgtgttgtg gagtgggaag cggcgattgc tgatgccata gtcgaaaccc atgacaggat
     5221 cgtaggaaaa acctggcggg aagcgaagcg ccagcatgac gaaacaattt ccggctctaa
     5281 agccacactc acggatacga tccgtacctt caccgcgctg ggagcttcgt tgcttgaggc
     5341 ccgcagtgac ggaaccccgc tggagatggc tgtcgccagt tcggttgcat gggaccggct
     5401 cgctcaactg gtagcgacag ggactcaact cagcaacacg ctagccgatg agcctcttgc
     5461 atatgtcggg cagggatacc atcgctttcg tcgttatgcg ccccgcatgt tgcgctgtct
     5521 gaagctcgaa gccgcgccgg tcgccggacc attggtagca gcagctttgt cgatcggaga
     5581 gatgaaaggt gttgcatcgc cagaaaggcg tttcctgcgg cccagctcca aatggaaccg
     5641 tcatttacga gctcaggaaa aaggagatac ccgtctttgg gaagtggcgg tactctttca
     5701 cctccgggat gcttttcgtt ccggagatgt ctggctcgct cattcgcgcc gctatggtga
     5761 cctcaagcag gtactggtgc cgatgatcgc ggcgcaggaa aatgcaaaac tggccgtgcc
     5821 ttccaaccca caggattggc tggcagacag aaaggcgcga ctcacgatcg ctcttaagcg
     5881 gctggcccgg gctgcccgta acggcactat tccgcacggt agcatagaag atggaacgtt
     5941 gcggatcgac aggttgacag cagacgtgcc ggatggtgcc gaggcactca tactggatct
     6001 gtatcgccga atgccgtccg ttcggattac cgacatgctg cttgaagttg atgcagccct
     6061 tggtttcaca gatgcgttta cccatctgag aaccggggct ccatgtcgcg accggatcgg
     6121 tctgctcaac gtcctgctcg ctgaagggct caatctgggc ctgcgtaaga tggcggaagc
     6181 tacaaacacg catgattact ggcagctctc acgccttgcc cgctggcatg ttgaaagcga
     6241 agccatgaac caggcattgg caattgtggt ggccgcgcag ggtaaactgc cgatgtcacg
     6301 cgtctggggg atgggcacgt cagcatcgag cgatggtcag tttttcccga cagcgcggca
     6361 tggcgaagcc atgaacatgg tcaatgccaa atatggttct gttcccggcc tcaaagcgta
     6421 tactcacgta agcgaccagt tcgcgccatt cgcttgtcag tcgatcccgg cgaccgtgag
     6481 cgaggcaccg tatattctcg atggactact gatgaacgag gtcggtcgcc atgttcgcga
     6541 acagtatgcc gatacagcag gattcaccga ccatttgttc ggagccagta gcctgctcgg
     6601 ctacaatctc gttctgcgaa tcagggatct gccatcgaag cggttgtacg tatttaatcc
     6661 cgatacgacc cccagggagt tacgcaagtt ggtaggtgga aaagcccggg aggatcttat
     6721 cgttgcgaac tggcctgata ttttccgttg tgccgcgacg atgaccgctg gcaaaatcag
     6781 gcccagccaa ctcctgcgca agctcgcttc ttacccacga caaaacaacc ttgcagttgc
     6841 gcttcgtgaa gttggtcgta ttgaacggac ccttttcatt attgagtgga tcctggatac
     6901 ggacatgcag cggcgtgctc agatcggtct taacaaggga gaggcccacc atgcgctcaa
     6961 aaatgcgctc cgtatcggga ggcaggggga aattcgcgat cgcacgacag aggggcagca
     7021 ctaccgaatc gctgggctca atttattgac tgcggtgatc atttactgga ataccgtcca
     7081 tcttggtcat gccgtcacgg agcggcggaa cgaagggttg gatgttcccc ctgaatttct
     7141 tccccacata tccccattgg gctgggcgca cattctactg actggcgaat atctttggcc
     7201 caaggaaccg aaagcttagg gtgtcatttc gccctcagcc ggaaccgacc ccaaattgag
     7261 tgttgctctg tggataactt gcagagttta ttaagtatca ttgcagcaaa gatgaaatca
     7321 atgatttatc aaaaatgatt gaaaggtggt tgtaaataat gttacaatgt gtgagaagca
     7381 gtctaaattc ttcgtgaaat agtgattttt gaagctaata aaaaacacac gtggaattta
     7441 ggtaatagtt aaagcttgtg cctgattgtt gccacaattg gtcagcagtt tgctagggaa
     7501 tgagagggca gagaatgata agcttagcac ttaatttgta tggatatatt gcattaagca
     7561 aggggacgtt atgcgtgtat tagccttatc ggctgtgttt ttggtggcat cgattatcgg
     7621 aatgccagcg gtagcaaagg aatggcaaga aaacaaaagt tggaatgctc actttactga
     7681 acataaatca cagggcgtag ttgtgctctg gaatgagaat aagcagcaag gatttaccaa
     7741 taatcttaaa cgggcgaacc aagcattttt acccgcatct acctttaaaa ttcccaatag
     7801 cttgatcgcc ctcgatttgg gcgtggttaa ggatgaacac caagtcttta agtgggatgg
     7861 acagacgcgt gatatcgccg cttggaatcg tgaccatgac ttaattaccg cgatgaagta
     7921 ctcagttgtg cctgtttatc aagaatttgc ccgccaaatt ggtgaggcac gtatgagtaa
     7981 aatgctgcac gccttcgatt atggcaatga ggatatctcg ggcaatgtag acagtttttg
     8041 gctcgatggt ggtattcgca tttcggctac ccagcaaatc gcttttttac gcaagctgta
     8101 tcacaacaag ctgcacgttt ctgagcgtag tcagcgcatc gtgaaacaag ccatgctgac
     8161 cgaagccaat ggcgactata ttattcgggc taaaacggga tactcgacta gaatcgaacc
     8221 taagattggc tggtgggttg gttgggttga acttgatgat aatgtgtggt tttttgcgat
     8281 gaatatggat atgcccacat cggatggttt agggctgcgc caagccatca caaaagaagt
     8341 gctcaaacag gagaaaatta ttccctagaa gtggttagcg cgtatttgtg tgaaatagcc
     8401 gtcatataag ctgtaaagat atatggacaa aatgctgatg ctcgatgcgc ttgtagttaa
     8461 tggctcatac ttaatcggtc aggcctaatc gacttaagct ccgcgcgtta actttcagcc
     8521 tgagcattag catcacattc ctaaacgtaa aaagcctagc caatttgcta ggcttttttg
     8581 ttatctcaat ggccatggtc actcaacatg ggggtgtgaa taaaggagtg gcgatgtcag
     8641 taaaggtgaa tgccgctgcg ataaagtgcc tagcccatca aggattgtag gctttcttgg
     8701 ctgctaaagc gtttgacgaa aaagtccaag aaggcgctga tattggttct ccatcatttt
     8761 catctaccgg tttactatag gtcaaccagg ccgtccttgc cggcgcttgg gacgcagacg
     8821 atggcatcaa atgcctcgct caaattcgtt tctacagagg cgctttggga ccgcattcgc
     8881 tttgctttca tgggggcatc tgtcaatgtc agacatgaat cctccgatat aggttgattt
     8941 ttgatctctt caacacttgg tgttgtccga ctagagagac ttatatggat cagtattctt
     9001 gcactgacaa tggtgccact caatcccaag gatcgaccta gttagaggcg tagatcaagc
     9061 aattcaaaac agcgcagaag cagaaggagg tggaaatatg ttccagggtg aaaggttaca
     9121 tgcaactaac gttgctctct aggatgtgcc tgctgtaacg tattgtccag aaaagacaaa
     9181 cccccgttat cgcttgcatc ttggcggagc tgcgaaaaac gggggccgtg acgaatcaca
     9241 agagaagtct atcacatgga tcatgccaaa caaccccacg ccgccaataa gcgcggtggt
     9301 gc
//
