LOCUS       NZ_CP069187             3247 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Enterococcus faecalis strain L11 plasmid pL11, complete sequence.
ACCESSION   NZ_CP069187
VERSION     NZ_CP069187.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17615183
            Assembly: GCF_016812095.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Zorzi,F.M., Almeida,L.M. and Gilmore,M.S.
  TITLE     Enterococcus faecalis L11
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Zorzi,F.M., Almeida,L.M. and Gilmore,M.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2021) Clinical Analysis, USP, Prof. Lineu Prestes,
            580, SP, SP 05508000, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 8.0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 348.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016812095.1-RS_2024_03_30
            Annotation Date                   :: 03/30/2024 05:10:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,731
            CDSs (total)                      :: 2,652
            Genes (coding)                    :: 2,623
            CDSs (with protein)               :: 2,623
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 29
            CDSs (without protein)            :: 29
            Pseudo Genes (ambiguous residues) :: 0 of 29
            Pseudo Genes (frameshifted)       :: 13 of 29
            Pseudo Genes (incomplete)         :: 10 of 29
            Pseudo Genes (internal stop)      :: 10 of 29
            Pseudo Genes (multiple problems)  :: 3 of 29
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069187.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="L11"
                     /isolation_source="Rectal swab"
                     /host="Swine"
                     /db_xref="taxon:1351"
                     /plasmid="pL11"
                     /geo_loc_name="Brazil: Distrito Federal"
                     /collection_date="2013"
     gene            complement(48..1295)
                     /gene="mobV"
                     /locus_tag="JO410_RS13560"
                     /old_locus_tag="JO410_13560"
     CDS             complement(48..1295)
                     /gene="mobV"
                     /locus_tag="JO410_RS13560"
                     /old_locus_tag="JO410_13560"
                     /inference="COORDINATES: protein motif:HMM:NF041497.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_203361396.1"
                     /translation="MAHLKKNTRASLSGLSIHFERKTDNHSNKEIDISRSHLNQDLMQD
                     NSNMVERFNERLEDVYCMNRKDVKALGTWVVTLPDELKDLSHDKQQEFFNETKNFLDER
                     YGKENAVAAIVHYDETTPHLHYAFVPVTFDEKKDRLKVSAKEVLNRKDLQTFHDDLNKH
                     LKEKLPFYEKGILNDQTLPFQNVEEIKKYNETYQEMKQALEQKKAVIRMKNDALEPFEK
                     SLKRINDTEKMFDTIDKNAKKGFGQVVIKSSDFEKLKTQAVENQVLANANEKEMYILKD
                     KNKKLEKDNRMLSTKLDHKTQKNESLTKKNNELTKVKNDGHKFRELVKRDLEKQYNIVD
                     LTEKEVNALSVVKAIDEDIKPKNKEEGKSWLDDLNNGKGTRIEESMLEKALEKALELYR
                     EIVQKVERMMGMNIPL"
                     /name="mobV"
     gene            complement(1611..1829)
                     /locus_tag="JO410_RS13565"
                     /old_locus_tag="JO410_13565"
     CDS             complement(1611..1829)
                     /locus_tag="JO410_RS13565"
                     /old_locus_tag="JO410_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_203361397.1"
                     /translation="MAEKYIGKKNWLVELIGEKVAISFLDGKKMTGKIIDVTAYDIILV
                     NKKIENPIVILKHSVKYVYKLSDKQED"
                     /name="hypothetical protein"
     gene            complement(1829..2800)
                     /locus_tag="JO410_RS13570"
                     /old_locus_tag="JO410_13570"
     CDS             complement(1829..2800)
                     /locus_tag="JO410_RS13570"
                     /old_locus_tag="JO410_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_203361395.1"
                     /translation="MSKNGTIVNEWEKLAALSNRRLSVGESVKHIKMNWSIDRITIVGK
                     LKESILYNTSNGFICIDFEQLMRLNEGQGYLESVSSNGWKLVDKFGENIAYIEILKFQE
                     GKGRIDFNPSKINNFLASSMKNFIHDLFLEPHFSRADIACDMIDVPNDFITQYRVVDPI
                     SFKPIYGRSGKLETAYWGSRSSERQIRLYNKKLEQELKRKIVPQEIETWWRLELQLRRG
                     KANNWYSMVQDSLDSFASTHFLPTTVKPMDKVMLDGLVANQNNWSIISRDLKYRLRKIL
                     KEESLNDELTNHLRETFFEKSEELKNELDTWLQGLDVTEEEV"
                     /name="replication initiation factor domain-containing
                     protein"
ORIGIN
        1 ctaaacgaaa taaacgctaa aacaccttag aacgcatttt atgagcttta taaaggtata
       61 ttcataccca tcatacgttc cactttttga acgatctctc tatataattc aagtgctttt
      121 tcaagtgctt tttcaagcat tgattcttct attctcgtgc ctttcccatt gttcaaatca
      181 tctaaccagc ttttaccctc ttctttattt tttggcttaa tatcttcatc aatcgctttt
      241 acaacagaca aagcattcac ttctttttct gttaaatcaa ctatattgta ttgtttttct
      301 aaatctcttt tcaccaattc tctaaattta tgaccatcat ttttaacttt tgttaattca
      361 ttattttttt tagttaatga ttcatttttt tgtgttttat gatctagttt agtagacaac
      421 atacgattat ctttttctaa ttttttattt ttatctttta aaatatacat ctctttttca
      481 ttcgcattcg caagaacctg attttctact gcctgcgttt ttaatttttc aaaatcactt
      541 gattttataa cgacttgacc aaatcccttt ttagcatttt tatcaatcgt atcaaacatt
      601 ttttctgtat cattgattct ttttaatgat ttttcaaaag gttctaacgc atcatttttc
      661 attctgatga ctgctttttt ttgctctaat gcttgtttca tttcttgata tgtttcgttg
      721 tattttttta tttcttcaac attttgaaaa ggcaatgttt ggtcattcaa aattcccttt
      781 tcataaaaag gcaacttctc tttcaaatgc ttgtttaaat catcatgaaa tgtctgtaaa
      841 tcttttctgt ttaagacttc ttttgcagat acttttaatc tatctttctt ttcatcaaag
      901 gtaacaggaa caaacgcata atgtaaatgt ggtgttgttt cgtcatagtg tacaatcgca
      961 gcgacagcat tttcttttcc gtaacgttca tctaaaaaat ttttcgtttc gttaaaaaat
     1021 tcttgttgct tgtcatgaga taaatctttt aactcatcag gcaacgttac aacccaagtg
     1081 cctaatgcct taacatcttt tcgattcata caataaacat cttctagtct ttcattaaaa
     1141 cgttcgacca tatttgagtt gtcttgcatt aaatcttgat tcaaatgtga gcgactaata
     1201 tctatttctt tgtttgaatg attatcagtt ttgcgctcga aatgaattga taaaccacta
     1261 agagaagcac gagtattttt ctttaaatga gccatataat atccctccag ttcatagggt
     1321 ctttagaacc agtataacat agtttaaaat ctggtatagc ctgttatacc aaatttcatt
     1381 tggacaggct ctgccgagag ctattcgcct gcaagcaggc gagctaaaac gttcagaata
     1441 aatcgccttt aggcaattta tgatcttctc ttttttagcc ttacagccat gcaaattttt
     1501 tcaaatttac attcgctgct tttacttttt cggtggtggc acgattgcca atttccgaaa
     1561 aaataaaaaa agacaatcgc cttttgacga ttgtctaaaa aacaaattat ctaatcttct
     1621 tgcttatccg ataatttata aacatatttt actgagtgtt taagtatgac aattggattc
     1681 tcaatttttt tattcactag aataatatca taagcagtaa catcaataat tttgccagtc
     1741 attttttttc cgtccaaaaa actaatagca actttttcgc caattaattc tactaaccaa
     1801 ttttttttac caatatattt ttctgccatt atacttcctc ctctgttaca tctaaacctt
     1861 gtaaccatgt atctagttca ttttttaatt cttcagattt ctcaaaaaaa gtttctctca
     1921 aatgatttgt taattcgtca tttagacttt cttcttttaa aatttttctt aatctatatt
     1981 tcaaatcacg actaataata gaccagttat tttgattagc taccaagcca tctagcataa
     2041 ctttatccat tggtttaaca gttgttggta aaaaatgtgt actagcaaaa ctatctaaac
     2101 tatcttgaac catagaatac caattattag ccttgccacg tcttaattgt aattctaatc
     2161 gccaccaagt ttctatttct tgtggcacta tttttctttt taattcttgc tctaattttt
     2221 tattgtaaag tctaatttgt cgttcacttg accgactacc ccaataagca gtttctaact
     2281 ttcctgaacg tccataaatt ggtttaaatg aaattgggtc aaccacacga tattgagtaa
     2341 taaaatcatt aggtacatca atcatatcac aagctatatc cgctctagaa aaatgaggtt
     2401 ctaaaaataa atcatgtata aaatttttca tagaactagc taaaaaatta ttaattttac
     2461 tagggttaaa atctattctt ccctttcctt cttgaaattt aagtatttca atataagcaa
     2521 tgttttctcc aaatttatca actaatttcc aaccgtttga actgacagat tctaaataac
     2581 cctgtccttc attcaatctc attaactgtt caaaatcgat acaaataaaa ccgttagaag
     2641 tgttatacag tatggattct ttcaattttc caacaattgt aattctatca atcgaccaat
     2701 tcatttttat atgttttacg ctctcaccga cacttaaccg cctgttagat agggcggcca
     2761 atttttccca ctcattcacg atcgtgccat ttttactcaa atctagttcc tcctaaaaga
     2821 aaaaaagaga ttcttggaaa gaattttaaa agttcacgta acccttaaaa ttcattaaca
     2881 agaatctcta gtcttgaaga aacctaaaaa tattcgtata atgattgtga gttacggagc
     2941 tagtacaagt agtttccgta gtggtcgaga gtgattccaa cactgctcga cttttttttg
     3001 ctcttttttc tgtttttttt agtatatcac ttaaatttga aaagacaaaa aagtactata
     3061 aatagagcat gagggaattt cattccctca aactcccttg agcctctcct accgaaataa
     3121 aggacgctat cgcttattat ttcgtttagt tatcgactaa tgcaatctaa cacgaccgtt
     3181 ccgctaccag tcgccttaca ggctccattt tcgctcctct aacgtgcttt aacgattgcg
     3241 ccgataa
//
