LOCUS       NZ_CP069175             5596 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain KPWX136 plasmid pF, complete sequence.
ACCESSION   NZ_CP069175
VERSION     NZ_CP069175.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17372728
            Assembly: GCF_016812055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Zhou,Y. and Zhang,F.
  TITLE     Characterization of a Klebsiella pneumoniae strain KPWX136
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhou,Y. and Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2021) Department of Pathogenic Biology, Southwest
            Medical University, No. 319, Zhongshan Road, Luzhou 646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. December-2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:43:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,089
            CDSs (total)                      :: 5,968
            Genes (coding)                    :: 5,769
            CDSs (with protein)               :: 5,769
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 69 of 199
            Pseudo Genes (incomplete)         :: 140 of 199
            Pseudo Genes (internal stop)      :: 22 of 199
            Pseudo Genes (multiple problems)  :: 32 of 199
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP069175.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPWX136"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pF"
                     /geo_loc_name="China: Jiangsu"
                     /collection_date="2020-10-15"
     gene            join(5350..5596,1..80)
                     /locus_tag="JLH18_RS29670"
                     /old_locus_tag="JLH18_29665"
     CDS             join(5350..5596,1..80)
                     /locus_tag="JLH18_RS29670"
                     /old_locus_tag="JLH18_29665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            106..651
                     /locus_tag="JLH18_RS29675"
                     /old_locus_tag="JLH18_29670"
     CDS             106..651
                     /locus_tag="JLH18_RS29675"
                     /old_locus_tag="JLH18_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            717..1067
                     /locus_tag="JLH18_RS29680"
                     /old_locus_tag="JLH18_29675"
     CDS             717..1067
                     /locus_tag="JLH18_RS29680"
                     /old_locus_tag="JLH18_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1137..1403)
                     /locus_tag="JLH18_RS29685"
                     /old_locus_tag="JLH18_29680"
     CDS             complement(1137..1403)
                     /locus_tag="JLH18_RS29685"
                     /old_locus_tag="JLH18_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1387..1611)
                     /locus_tag="JLH18_RS29690"
                     /old_locus_tag="JLH18_29685"
     CDS             complement(1387..1611)
                     /locus_tag="JLH18_RS29690"
                     /old_locus_tag="JLH18_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(2278..2490)
                     /locus_tag="JLH18_RS29695"
                     /old_locus_tag="JLH18_29690"
     CDS             complement(2278..2490)
                     /locus_tag="JLH18_RS29695"
                     /old_locus_tag="JLH18_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2497..3006)
                     /locus_tag="JLH18_RS29700"
                     /old_locus_tag="JLH18_29695"
     CDS             complement(2497..3006)
                     /locus_tag="JLH18_RS29700"
                     /old_locus_tag="JLH18_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3041..3745)
                     /locus_tag="JLH18_RS30755"
                     /old_locus_tag="JLH18_29700"
                     /pseudo=""
     CDS             complement(<3041..3745)
                     /locus_tag="JLH18_RS30755"
                     /old_locus_tag="JLH18_29700"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3684..4028)
                     /locus_tag="JLH18_RS29710"
                     /old_locus_tag="JLH18_29705"
     CDS             complement(3684..4028)
                     /locus_tag="JLH18_RS29710"
                     /old_locus_tag="JLH18_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4018..4194)
                     /locus_tag="JLH18_RS29715"
                     /old_locus_tag="JLH18_29710"
     CDS             complement(4018..4194)
                     /locus_tag="JLH18_RS29715"
                     /old_locus_tag="JLH18_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4834..4992
                     /locus_tag="JLH18_RS30760"
     CDS             4834..4992
                     /locus_tag="JLH18_RS30760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     Typing          complement(4281..4357)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          4546..4943
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(2163..3694)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagataga ttcaattatt aatgacatga tggttctaaa ggaatatttc agagctaagt
       61 tttacgacga aataaaatag gcacgctata cggaggcgga gaattatgtt ttttaaacgg
      121 tatttaacgg agaaggaaat atacaaggca gcactcccaa ttgttaggct tcaagggaga
      181 gttaacactt cattacgttt tttatccaaa tactcagatg taaacaaaaa tggattaatt
      241 gcattgcatc ttaatttatt acattttatc tcatacaaca tacacggtgt agagtctctg
      301 aaaatcactc aagcaggcat aaacaaagct gtacaacaaa gccctcacgc agatgaaata
      361 aaaaatattg cgcaggcaat aggacgcaaa ggacctgagt acatcgaagg attaattaga
      421 aacccatggg ggaagaaaaa tgataatcca ctaatattta taatgagcat tgaagaaagt
      481 ataaaagaaa cccttaactc aggttatatg ttatatagag tagctcccat aactgagggt
      541 gaagcagctg ctttcatgga tgttcttaaa caattattat tatttgagga taattttcgc
      601 ctttttggta gagacttggt ttataaaata gaaaaattaa aaatcagata acaaagtatg
      661 gtgaacgaaa aatgaaagag aggatagtga aatgtgtaat accaagattc tcaactatgt
      721 attggttaat tgcgttccta gcatttatat tccccggtcg atcttttgct gacggttaca
      781 ccgcttacta ccgatgtaat ggtactcagg aagcatctct tactgttaaa gataacaaac
      841 taagtatggt tgtaggtctt tctaagttta ataggcaact ggatacttat agattcaaag
      901 aaaagggaat cgaagtagta atgcagagag ctgcttctga tgataactct gaggccataa
      961 tgtcttttat tatgcctgac gttaacaata tgaccttatt agttaaaaat aacggctaca
     1021 ttatcgttaa gacaacctgc tggttaacta aaaccgacaa acagtagaac acgtagttag
     1081 tcgagtattt accataatac ctgttgtcgg catcaaaaaa gcccgcatag cgggcttcag
     1141 tcgtaaactt cgcggcgatg tccaatcgtg gcggcaagga taacaagctc gtcatctcgg
     1201 atctcacaca gaacgcgata atctcccacc cgatagcgcc agaactcgcc cagctcacct
     1261 ttgagcggct tccctgactg gcgaggatcg gcagcaactg caatgcgtaa actcataaaa
     1321 tccacaatcc gtcgtgcgtt ctgtttgtcc atcttgcgta acgatttgag cgcccgatcg
     1381 gaatagttaa tcgtccagac caagtgactt cctcacgtct tcagacgaat gcactgcttc
     1441 atcaccacgg cgaacgcgtg cagcagtttc tgctgaaaga taataatcct ccaggtcttc
     1501 cagatgcgcc agtattgctt cccgcgcata aaacgtcttt gttctgccgg tttgcttcgc
     1561 cagcgagtcc agacgggact caatctcatc agataaccgg atagcaagca tataaacctc
     1621 ccgaacacca tttgctatac atgtatatca acagtaccac aatcaaccta aaagaacaaa
     1681 aaataatctt gttagcccct cttgataatc cgtaacgcgg tcaatatatc tagcttgagg
     1741 gctttcctct taactattga aaggataacg attatgggtc ttaagccggg tccgaaacga
     1801 attgccgatt caacagggga acctgataaa cgccagcgtg acaataaaaa aacgccggga
     1861 aatactgaca aattgaaacc cagcaaatct tcaaaaaaat aagtcagtag ttaaaagccc
     1921 tccggggctt ttttgtatct gccgttcacg ctgattaaac ataacacccg ttgcccgcac
     1981 cgccccggac gggttcgccg cgaacggggt ggcagtcagg taaaaccggc aacaaaccac
     2041 ccgataaacc gccccgttcg cgacagataa attttacctg gctgcatagc catgcagcca
     2101 ggtgaatgac atccggtttc agcgtaaatg cccgatgaga accctgtttt cctgcatttc
     2161 agcccagcga cggaccgtca tagcttcgct gccgggaacg cgcttccgtc agttcctttt
     2221 cgtgctgccg ttgctcgtgc gcccgttctg cggccacggc catctcatga cgctgcacta
     2281 ccggctcaaa cgctgcagtt gcccgctcaa gcgttccacc tgctgactca agcgcgtcac
     2341 gcgctcgctc agcgccgcgt tctcccgttg cataagccct gacattttcc gccattcccc
     2401 gaaggcgctc tcccactcgt ccagcctttt cgagtagtcc tgctgtagct gctctaatgc
     2461 gctcagcaac tgtttttcca gctcggtcat cggcacttac tccttcacca gtctcagcgc
     2521 gttctgcttc gaccgcccgt tcccgaccgt ccagctctgc gtcctgtcta cggccgtccc
     2581 cttcggcagc accagaaaac gtccgttgct gtcttccagg tacgtcacgc cccaggtctt
     2641 cgcgttcagc ttcgacagcg ctgcgttctg ccggtcgatt tcggccagat ttgacgctat
     2701 caagctcccc tgataccaca acaccccgct caggctggcg aacagcagtc ctgacaccat
     2761 cgtgatggtc agccatgtcc gcccgaccat acgcatcacg ctcaggcggt tcgactgcat
     2821 ggccgcgctc ataccctgct cgtgggcgcg gatggcggcg ctgattctct tcccgctttc
     2881 gctcagttcc gctctgacag acttctcgtg ctcgctgaat gcggctttca gcatttcgcc
     2941 ggtactctgc tgctgcgctt tcgatttctg ctctaagtcc ttcgccagcg ttaaaagact
     3001 gttcatagat ggctccctta agtcggatgt ttcgccctcc gtccgggtcg gcaatgctga
     3061 tgctgctctt tgtggtgcgc acgacctcaa aacctgcgct ttccagcgcc tcagtgacgt
     3121 cctgacgcgt tttaagctcc cctgacgagg cgagggccag taagccccgc gtaatagcct
     3181 ctgcggcttc ctgcttcgtt ttgggtaacg aagatggcgt caccagcgcg cgccggttct
     3241 ccggtgcgtt cgggtcgtgc agccccagtc gcccgttcac cacggtctgc cacgcatcga
     3301 tgcgcggacg gtctgcccgg tcgtagtacg gctggagacg cctgccggtc agcagttccg
     3361 tgttcggtac cagaaaattc agttccagcc gccccttgtc ccggtgttca acccacagca
     3421 cgctgtactg gtctttatcg agtccgggca tcagaacccg ctcgaagctc gccatcagct
     3481 tttcacgctg gccgggcggt aaatcctgtt cagcaaaaga caggacgccg gaggtgtact
     3541 ttttggcgta gggcgaggcg tcgataagct cccggacctc ctccggcttt ccctgcagga
     3601 cgatggcccc gtcgcgctgg cggtctttcc ccagcagata atccaccggc ccggcaccgc
     3661 cgcctcgccc tcgcggatga aacttaacga tcatcgtctg cccccttttc cagcacggca
     3721 tgccgcagcc gctcgagttc ggcatcaatg gccatcagcg cggccaccac ctgaacccgg
     3781 tcagcgcctg accactggcc gccgttaatt ttccgggcga tctggttgag gttgttcccc
     3841 atcccggcaa gctggcgcag caaaaccgga tcgatggacg gcagccgcaa cgaccgcgcc
     3901 gggcgcgtat cgagacaggt ttcccgcatc cacaccgcca gttgccttcc attgcagcgc
     3961 tccagcagct gctggtactc cccatcggtc acccggatac ctaaaaaacg ggtacgctta
     4021 accgccatcc tctcctccga tatccgcaaa atgtgaccgg gctgaacgct caagctctgc
     4081 catctgctcc cggcggtaat cttcctcctg tgcctgacgc atcagatttg ccatcactgc
     4141 cggatgccag gatctttttt ttacgacgag cggcttcttc ttcggcttct gcattatccc
     4201 tccttcgcaa acgccggacg gcacggaaaa gttttccggg tcgctccggc gttggacaag
     4261 cgccagcgcg tcagtggtgt tagcgggttt cggggcgcag ccctgaacca gtcacagcgt
     4321 ttacgctgtg tatactggct tactatgttg gcatgagagc acgattcatg aaagtgcctc
     4381 atgtggcagg tgtgatatgt ggctcaggag cgtccgcaat accatgcacg gacagatgaa
     4441 atgacgcctc ctcgctcact cggtagctgc gctcctgccg ttcggctgcg gcgggcgtga
     4501 ccgactcacg gataacggga taaagcaaag gcgctcaaat cagaaagggc cggagaaaag
     4561 gatgcggcgt tgccgttttt ccataggctc cgcctcccct gacgagcatc acagaaatgg
     4621 acgctcaagt cagaggagga cgaaacccga caggacttaa agatgccggt gatttctctc
     4681 cggcggctcc ctcgtgcgct ctcctgttcc gaccctgccg tttaccggat acctgttccg
     4741 cctttttccc tttcgggaag cgtggcgctt tctcatagct cacgctgctg gtatctcggc
     4801 tcggtttagg tcgttcgctc caagctgggc tgtatgcacg aaccccccgt tcagcccgac
     4861 tgctgcgccc tttccggtaa ctatcatctt gagtccaacc cggacggaca cgcaaaaacg
     4921 ccactggcag cagccattgg taactggata tatacgagag agatattgag aggtcttgaa
     4981 gtgggtgcct aactgcggct acactgaaag aacagttttg gtatctgcgg tctcgtatac
     5041 ccagttaccc ggttaagcag ttccccaact gacttaacct tcgatcaaac cgcctcccca
     5101 ggcggttttt tcgtttacag tgcaagagat tacgcgcaga aaaaaaggat ctcaagaaga
     5161 tcctttaaat gatcgtcagt tctgttatcc ccggagctcc atccatccca cggaaaaaca
     5221 cccgccactg atgccctcag cgcactgcac atcatgtaca aaccactccc tccctccttt
     5281 tttattgcgt ttcttaatgc gcaatggtag ctttgtgcgc aacaaaaaac gcaatagcaa
     5341 gggggggtaa tgagagaaag aacaaatatc atgctagata taggattaaa gcaatcctta
     5401 caacggcttt cgaataaaac caaaatgagt ctaagtgatc ttattaacca gagattatca
     5461 ggttcacttt cagttgaaga acgcattggc gaaaagcctg aatggatgag aaaccttgat
     5521 ccctatctcg ccaatcactt ttcaaatatt gagttaccgc tcccttcacc attaaaagaa
     5581 taccaagaaa aaatgg
//
