LOCUS       NZ_CP068728             3166 bp    DNA     circular CON 11-MAY-2024
DEFINITION  Bacillus cereus strain AFA01 plasmid p4, complete sequence.
ACCESSION   NZ_CP068728
VERSION     NZ_CP068728.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17369857
            Assembly: GCF_016774575.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Lu,J. and Chen,H.
  TITLE     Characterization of Bacillus cereus AFA01, capable of degrading
            gluten and immunotoxic peptides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Lu,J. and Chen,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2021) State-Key Laboratory of Food Science and
            Technology, Nanchang University, No. 235, Nanjing East Road,
            Nanchang 330047, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.9%x
            Sequencing Technology :: PacBio RS II Single MoleculeReal Time
            (SMRT) and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016774575.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 05:11:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,006
            CDSs (total)                      :: 5,853
            Genes (coding)                    :: 5,687
            CDSs (with protein)               :: 5,687
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 65 of 166
            Pseudo Genes (incomplete)         :: 117 of 166
            Pseudo Genes (internal stop)      :: 41 of 166
            Pseudo Genes (multiple problems)  :: 47 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068728.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="AFA01"
                     /db_xref="taxon:1396"
                     /plasmid="p4"
                     /geo_loc_name="China: Nanchang"
                     /collection_date="2017-11"
     gene            61..561
                     /locus_tag="JM957_RS29720"
     CDS             61..561
                     /locus_tag="JM957_RS29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182216.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001182216.1"
                     /translation="MQPERPLKKVSKVTKNKIIDINTGEIQEEEEIINAYIDKEPDYVK
                     IYLKDIVALNDLPKGLDRVINVLLKVMSYDNMIILNSFIKKQMAQELGYKTVQVLNNNI
                     NKLVNAEILFRKGTGTYQVNARYFGRGHWHDIQEIRLQQVYSTKGRELKTEITYKNDEP
                     SQN"
                     /name="replication/maintenance protein RepL"
     gene            complement(575..1819)
                     /gene="mobV"
                     /locus_tag="JM957_RS29725"
     CDS             complement(575..1819)
                     /gene="mobV"
                     /locus_tag="JM957_RS29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562244.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000951644.1"
                     /translation="MAHVQKYAKGNVQGLSIHWDRKTENHSNQDIDNERSHLNYDLCEK
                     EGDTLSRMNDRLSEVHCLNRKDVKVCADWAVTLPESLKDASEKEQREFFEKTYEFLANR
                     YGGEKNVLSANVHNDETRPHMHFAFMPVVWDEKKQQAKVSAKEVLTRKDLKTFHQDLDE
                     FLKKEIPQIYKEGILNDKTIGVDTVKDLKRYSEEIQKQKDVMDAEVKVKERELEGKIQS
                     LDKELESKKNEFLNLSEALPSKINIKVKGKEKKTEVVKTGFLKSETVTTETGNWIVSDS
                     EMRRMQKVLSDANFVKEDYERLQRTDLVKENKELHDRVDSLADGYVKAINENTDLYEKN
                     RELRKEISSLKAHVKDLKENVKVLYHNTKKVLGEHFKAFRGLVKNELDIKGVDNQFDCE
                     YKKEIKKQRGYNMER"
                     /name="mobV"
     gene            complement(2187..2420)
                     /locus_tag="JM957_RS29730"
     CDS             complement(2187..2420)
                     /locus_tag="JM957_RS29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="WP_046199669.1"
                     /translation="MTKLEELEKDFNQMNLDLKAIQHDMKSLEVRILVAEKDVLTINKQ
                     LDKISANTTWILRLIISGLLTGVLGVVAKNLL"
                     /name="hemolysin XhlA family protein"
     Typing          complement(579..1819)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttttaaaat acccgattta taatttaatt aaattataaa tcgggtagga ggtagatttg
       61 atgcaaccgg aaagaccctt gaaaaaagtt tctaaagtta caaaaaataa aataatagat
      121 ataaatactg gtgaaattca agaagaagaa gaaattataa atgcttatat cgacaaagaa
      181 ccagactacg taaaaatata tttaaaagat attgtagcct taaatgattt acctaaaggt
      241 ttagatagag ttattaacgt tttattaaaa gtaatgtctt atgacaatat gatcattcta
      301 aatagcttta taaaaaaaca aatggcacaa gaactaggat ataaaacagt acaagttctt
      361 aataacaaca tcaacaaact tgtaaatgca gaaattcttt ttagaaaagg tactggtaca
      421 tatcaagtaa atgctcgtta ttttggtcgt ggccattggc acgatataca agaaatccga
      481 ttacaacaag tctattcaac taaaggaaga gagcttaaaa cagaaataac atacaaaaac
      541 gacgaaccgt ctcagaatta attctgagac ggttttaacg ttccatgtta tatccacgtt
      601 gtttcttaat ttctttctta tattcacaat caaattgatt atctacacct tttatatcca
      661 attcattctt aacaagtcct ctaaacgctt taaaatgctc tccaaggact tttttcgtat
      721 tgtggtataa aacctttaca ttttccttta aatcttttac atgggctttt aacgagctta
      781 tttccttacg taattctcta ttcttctcat acaaatctgt attctcatta atagctttaa
      841 cataaccatc ggctaaacta tcaactctat catgtaattc tttgttttct ttaactaaat
      901 ctgtacgctg caaacgctca taatcttcct ttacgaaatt tgcatcgctt agaacttttt
      961 gcatccttct catttcacta tcagaaacaa tccaattccc agtttctgta gttactgttt
     1021 ctgattttaa aaaacctgtt tttacaacct ctgttttttt ctcttttcct tttactttga
     1081 tattaatttt tgaagggagt gcttcactca aattcagaaa ctcatttttt ttactttcca
     1141 attctttatc taatgattga attttcccct ctaactctct ttctttgacc tttacttccg
     1201 catccattac atctttttgt ttctgtattt cttcagaata ccttttcaaa tctttaaccg
     1261 tatcaacgcc aatcgtttta tcgtttaaaa taccttcttt gtagatttgt ggtatttctt
     1321 ttttcaaaaa ctcatctaaa tcttggtgaa atgtttttaa atcttttctt gttaaaactt
     1381 ctttagctga aacctttgcc tgttgctttt tctcgtccca aacaacaggc ataaaagcaa
     1441 aatgcatatg cggtcttgtt tcgtcattat gtacattggc tgacaacaca tttttttcac
     1501 caccataacg gttcgctaaa aattcatagg ttttctcaaa aaattcacgt tgttccttct
     1561 cagaagcatc tttcaaactt tccggtaatg taacagccca atctgcacag acttttacat
     1621 cttttcgatt caagcaatgc acttcactta accgatcatt catgcgtgaa agtgtatctc
     1681 cttctttttc acataaatca taattcaaat gtgaccgttc attatcaatg tcttgattcg
     1741 aatgattttc tgtttttctg tcccagtgga tggacaatcc ttgaacattt ccttttgcat
     1801 atttttgaac gtgagccatg agcaagaccc ccccttttgg taactagttt aacatgggga
     1861 tagaagaaaa accaaatttg gtctagcacg ttataccaaa tttcatttgg acgtgctctg
     1921 cgaggctctt tggctccgcc aagccatgct ttgcctggca aaaaacaaac cttgggcttt
     1981 gcccaaaccc actggggaaa ctcttcccca gaccccctca atcaaactcc ccaacccctc
     2041 aatccttgag gggctccctc gccggttagc aggaacaaac gattgtttgt tcaatgccaa
     2101 gagggtttgg gtgtagtacg gttggtcaag tcaaatactc ccttcgcttt gctaggtccg
     2161 ttttgattga ccaacaccct tttttcctat aacaggtttt tagctactac accaagtact
     2221 cctgttaaca atccgcttat gatcaatcgt aaaatccatg tagtatttgc actaatttta
     2281 tctaattgtt tattaatagt taaaacatcc ttttcagcta ctaatattct tacttctaaa
     2341 cttttcatat catgttgtat agcttttaaa tctagattca tttgattaaa atctttttct
     2401 agttcctcca attttgtcat ataacatact tctcctttta aattaatttt aaatatatta
     2461 tatgtatatg gtaattagag aataatgata taaaaagaga actcaaatct attattaaga
     2521 tttaagttct ctttaaagat aagggaatta tacgaaagcg taatatagca tatgcttatc
     2581 ctttttaata tgcatctagc aaaaacacag tttctataaa tgtgctgttc taagttcatt
     2641 ctctccttgt ttttgggtgt gagtgctggc tgggggtttg ggggctagcc ccctggatct
     2701 taacgtaaat gaatatggaa taagcggatt gtttagaacg atagagcaag gcggttcggc
     2761 aggtacaatt gctagtccaa gtcgaacaat ctaaatcctc gtttttggtc tgttcggctt
     2821 ggcgacatgc tcacacctgc cgaatttcac tatgcaatag caaaagagtg gagatttttt
     2881 gaatggtttt ctcaaaaaat actactcgac tattgcatag tgaaaagccc ttttaaattt
     2941 tataaaataa tagaatagaa gggagaacat aggttgaacc gttgatatga ctggatttct
     3001 aggaaaatac tataatttgt gggattataa gttataattt gtgggattat aaccgtaatt
     3061 tgtggagtta tataccgtaa tttgtggagt tataaattaa atttattttt tatatcattt
     3121 ataatttgtg ggattataaa tgtgaaatcc tataaaaaat attgca
//
