LOCUS       NZ_CP068727             9190 bp    DNA     circular CON 11-MAY-2024
DEFINITION  Bacillus cereus strain AFA01 plasmid p3, complete sequence.
ACCESSION   NZ_CP068727
VERSION     NZ_CP068727.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17369857
            Assembly: GCF_016774575.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Lu,J. and Chen,H.
  TITLE     Characterization of Bacillus cereus AFA01, capable of degrading
            gluten and immunotoxic peptides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   Lu,J. and Chen,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2021) State-Key Laboratory of Food Science and
            Technology, Nanchang University, No. 235, Nanjing East Road,
            Nanchang 330047, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.9%x
            Sequencing Technology :: PacBio RS II Single MoleculeReal Time
            (SMRT) and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016774575.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 05:11:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,006
            CDSs (total)                      :: 5,853
            Genes (coding)                    :: 5,687
            CDSs (with protein)               :: 5,687
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 65 of 166
            Pseudo Genes (incomplete)         :: 117 of 166
            Pseudo Genes (internal stop)      :: 41 of 166
            Pseudo Genes (multiple problems)  :: 47 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068727.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="AFA01"
                     /db_xref="taxon:1396"
                     /plasmid="p3"
                     /geo_loc_name="China: Nanchang"
                     /collection_date="2017-11"
     gene            join(9059..9190,1..537)
                     /locus_tag="JM957_RS29665"
     CDS             join(9059..9190,1..537)
                     /locus_tag="JM957_RS29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002081870.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_215253610.1"
                     /translation="MRYSDIKVGNIYYADLNPIRKYEFGDNHLSIILSKGKDKRTVTIV
                     SLTSKSSGLGQNKMNLGIVSGLPKRLVEDRSGNPINTYVVLDQVRTVSANRIQYIKDGK
                     KTDGTDNYIECPVDAFSFSKIVCELADLRIADLNDEDAIGEYHKKTFFNYCVKKMIDLT
                     YDIIKGRGIVADKKEEVIYFYNNALAMEKGFLIDNYLKPHDIKNKVLEKFNEIVLMSVK
                     "
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            1168..2373
                     /gene="mobV"
                     /locus_tag="JM957_RS29670"
     CDS             1168..2373
                     /gene="mobV"
                     /locus_tag="JM957_RS29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_050267888.1"
                     /translation="MSRFMVCHMKKFKMTDVKGLQIHNQREKESHSNPDIIQERTEQNY
                     DLIHGKENVDYKKLIQDRIQDGVVSDRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSAQRFKAVTLQQEIEKLEQEKNEIDSRLHDLKL
                     SLDKAKSVDEIPVKEKGGFIRSKTVEIALEDFESIKVLAKSSETLREENKRLKNEKVKS
                     EYEKDNLYKEQRFLERKVTDLKRENEGLKGENDFLKKTLDRVKDLYKEKLPEFAGMIGY
                     VKASILDKMNRKFLKRHFAGDDEVSGAQKFLNHKQEHEEQQKRLKQVRRSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            2405..2686
                     /locus_tag="JM957_RS29675"
     CDS             2405..2686
                     /locus_tag="JM957_RS29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065382350.1"
                     /translation="MEYFHSFGVNFINTVKDLAEKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKGMNEQIMGLGEGLSDISKSLSDMSESIALVSLDDKE"
                     /name="hypothetical protein"
     gene            3021..3782
                     /locus_tag="JM957_RS29680"
     CDS             3021..3782
                     /locus_tag="JM957_RS29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000957234.1"
                     /translation="MLTNTFLSKVYFALDYSDFFEIDDFSVTLPEEKSYSQELIIKYLF
                     DETCYFHTYINGSSEFRYECSPGKVMKIQKGNVSGVDIYTTEIRDWVKRMEDEFKTTPL
                     ARRVMKNQSQVEELIDKVDSYFVENGLDSNDKFSHPEMEEMESKLEDFKALIEERLAEQ
                     ITEKDFLKQEVSKLFKEIDFLKKQVQILTKKNWFKAFCSKTGKFIRNNPETTKAIAHTA
                     KECLPESIKMSIPEGATEVMNTLLEEQKVEN"
                     /name="hypothetical protein"
     gene            4014..4277
                     /locus_tag="JM957_RS29685"
     CDS             4014..4277
                     /locus_tag="JM957_RS29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000085833.1"
                     /translation="MSRERVKIKFKSEDIKAEIEGYSDMPYWNHDLKLEFILRLLNTVD
                     EKQFLESQNHDQLDYYTKEMIKMIEASLGFDGLYRLVYGDEL"
                     /name="hypothetical protein"
     gene            4401..4607
                     /locus_tag="JM957_RS29690"
     CDS             4401..4607
                     /locus_tag="JM957_RS29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000030440.1"
                     /translation="MSKANYVTVGRKPKDGKNPKIPETFRLPKETSERIKGLVSSGRFS
                     TKAEIAEKAFEFYFSYLEKENGK"
                     /name="hypothetical protein"
     gene            4769..6148
                     /locus_tag="JM957_RS29695"
     CDS             4769..6148
                     /locus_tag="JM957_RS29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000650.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_050279502.1"
                     /translation="MNDVLKSNLLDITIDKLSIVADFKEGIKKEEFIRMVTSSNMPYSL
                     QANRSKNFGYEEVLKCDSVDCGYIELAGELKKASVDLPKLISHRIRLEIQIEQEKKARE
                     VGESLLSNAEYQGLFESLADVKELIAETDEDGRLTDERKLKRHERQIELTIEKLKGEIV
                     ALKSEIDEQELATGFKSEAMEKIHEEHVQILLNTLENKEKLIEVKRDELLRNAEMFLQL
                     DANGFLPNNGKKKNDRLLKDVRFEFNPKHMAYNRVVDEAIRLVLGLLENIEVTGIHIAC
                     DYPVKIADLKIKDLSSKSECIYLDRDKKIETMYIGKRGSDNHLCIYNKKRENEENETLD
                     QYPDVEHVTRFEARLRKKKAKEWIDSEYNPFDSIIVGDLEKVDNSKIKVNDRIVLEAIM
                     NDYHGRYFGMMDKDQKSNWRKKIKQHVPKLFDVAGDYEKKKVLLTGELAELMKNKPSKQ
                     K"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            6596..6802
                     /locus_tag="JM957_RS29700"
     CDS             6596..6802
                     /locus_tag="JM957_RS29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000947848.1"
                     /translation="MLSEQVIYYWNDELQYYYLAEKKNGSYKFVQPLKKLELDDISDPL
                     EREQMKKMLEIKKRMSETKGNWF"
                     /name="hypothetical protein"
     gene            complement(7504..7767)
                     /locus_tag="JM957_RS29705"
     CDS             complement(7504..7767)
                     /locus_tag="JM957_RS29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874968.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPSELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(7781..8068)
                     /locus_tag="JM957_RS29710"
     CDS             complement(7781..8068)
                     /locus_tag="JM957_RS29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000323835.1"
                     /translation="MAYLTAKKVKGNIYFYVSQYVGTQPYYSKHYRYINIYSIGNQKIA
                     LERIAMWLLDNNRIPKELIEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     gene            8257..8484
                     /locus_tag="JM957_RS29715"
     CDS             8257..8484
                     /locus_tag="JM957_RS29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000780193.1"
                     /translation="MKLVNNIRMIMAQKNIDNIAELIRITGVSRNSINKLWHNESVSSL
                     RLDTLIAICEKLDVKLSDLIEYIPGDIESK"
                     /name="helix-turn-helix transcriptional regulator"
     Typing          4770..6117
                     /name="rep_cluster_268"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.344"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          1169..2370
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttctctta cttcgaaaag ttctggtttg ggacaaaata aaatgaattt aggtatagta
       61 tcgggattgc ctaaacgttt agttgaagat cgaagtggga atccgatcaa tacatatgtt
      121 gttttggatc aagttcgtac agtgtccgct aatcgtattc aatatataaa agatggcaaa
      181 aaaactgatg gtactgataa ttatattgaa tgtccagttg atgcgttttc tttttctaaa
      241 attgtatgtg aattggcaga tctaaggatt gctgatttga atgacgaaga cgcaattggt
      301 gaatatcata agaaaacttt ttttaattat tgtgtaaaga aaatgattga tttaacatac
      361 gatattatta agggacgagg gatcgtcgcg gataaaaaag aagaagtaat atacttttat
      421 aacaatgcgt tagcgatgga aaaaggtttt ttaattgata attatttaaa accacatgat
      481 ataaaaaata aggtgttgga aaaattcaat gagattgttt taatgtcagt aaaataatta
      541 taagtgttga tttttattat tatttttgat acagtgtggt tagaagaagt agcgttagtt
      601 aatactaatt gcgaaagagc tgtgatggct caaccgtaga atttagggtt gtgatgaccc
      661 gaacaatagt tggcgagctg tgatggctca accatagaga gacctttctg taaaaagaaa
      721 ggtctttttg attgttattt atgaaaatga tggcttcctt tgttttgagg gttttaactc
      781 taaaataaag gggggctttt tttattgggg attactttta aagagatatg aactttgtgt
      841 gggggatgtc aacggtaccg gaccgaaggg aggataagga gttgacatct ttactacaac
      901 aaaaccctct tggttttccc aaaccgaacg tttggggatg cctaccggcg agggagcccc
      961 tcaaggattg aggggttggg gagttcgatt gtaaggggtc aaggggaagt ttttcccttg
     1021 tgggtttggg caaagcccat ggttttttgc catgattgtc atggctcggt ggtagccgaa
     1081 atgccccgta gggcacacgt gtttacgcag taaactgtag tgtgctacag tttacatgga
     1141 actaccaccg aaaaaggagc gaagcccatg tcaagattca tggtttgtca tatgaaaaaa
     1201 ttcaagatga cagatgtgaa aggattacaa atacataatc aacgtgaaaa agaaagtcat
     1261 tcaaatccag atattattca agaacgaacg gaacagaatt atgatttgat tcatggtaaa
     1321 gagaacgtgg attataaaaa actaattcaa gatagaattc aagacggggt tgtatctgat
     1381 agagccatta ggaaagatgc tgttgtttgt tgttcgttta tgatttcggc tagtcctgat
     1441 tatatgaatt ctttatctca agcggaacag agacgattct ttgaagagtc tgtttgtttt
     1501 ttaaaggcaa ggtatggaga agagaacttt gtatatgctt cagtgcatgt tgatgaaaaa
     1561 acacctcata tgcatgtagg aatggttccg gttaatgaaa aacaaaaact atcagcgtat
     1621 tcatttttta aaaacaaatc agagcttcat gatctacaag ataaaatcta tgaacatgtg
     1681 aaagaaaagg gatttgatat agagcgtggt gtttctagtg acaggaaaca tttatcggcg
     1741 cagcgcttta aagcggtgac tttgcaacaa gaaatagaga agttagaaca ggaaaagaac
     1801 gagatagata gtcgtcttca tgatttgaaa ttatctttag ataaagctaa aagtgtagat
     1861 gagatccctg ttaaagagaa aggtgggttt atacgctcta aaacggtcga gattgcatta
     1921 gaagactttg aaagtataaa agtattggct aagtcttctg aaacgcttag agaggaaaat
     1981 aagcgcctta aaaacgaaaa ggttaagagt gaatatgaaa aagataattt atataaagaa
     2041 caacgttttt tagagaggaa agtaactgat ttaaaaagag agaatgaagg gttaaagggc
     2101 gaaaatgatt ttcttaagaa aacgttggat cgagtgaaag atttgtataa ggagaagttg
     2161 cctgaatttg caggaatgat tggttatgtt aaagcgagta tactggataa gatgaatcgc
     2221 aagtttttaa aacgacattt tgctggtgat gacgaagtaa gtggtgctca aaagttcttg
     2281 aatcacaaac aagagcatga agaacaacaa aagagattaa aacaagtgcg aagaagccaa
     2341 cagaaaaatc gggaccaggg attggagaga tagtgtgggg acattattta atcaacaacc
     2401 aaggatggag tattttcata gctttggagt gaactttata aacactgtga aggatttggc
     2461 tgagaaaaat aatttaacga ttgaagagac ttgcaggatt ttggaattga ctatgaaaat
     2521 tgatgactat gatcgtaagg atgaacaatt agctggtatt ggagaactta tcaaagggat
     2581 gaatgaacaa attatgggac ttggtgaagg cttgagtgat ataagtaaaa gtttgagtga
     2641 tatgagtgaa agtattgcgt tagtatcttt ggatgataaa gagtaatagt gtgttggggg
     2701 actctatggg agttcccttt attctttaga agttagcatt agtggaactt atttttgtga
     2761 aatatagcga ggattgtaga gccttgtata acgtcctcgt aaattgttga tgtgaatata
     2821 tggggatatt atacaagggg attggggatg gctcttgttt ttaaaaaaag gaaattgaaa
     2881 taagtgtttt tagatgtgta ttatgttgtt agatattggt atatttaatt ttaatgagga
     2941 tagtaaagtc ttgtgtgact ttattgtttt tattttgtgt atttttattg ttgggtatat
     3001 acaagttggg aggatttttt gtgttaacta atacgtttct aagtaaagtt tattttgcgt
     3061 tggactatag tgatttcttt gaaattgatg atttttcagt aacacttcca gaagaaaaaa
     3121 gttattctca agaattaatc ataaagtatt tgtttgatga aacttgttat tttcacactt
     3181 atattaatgg atcatcggaa tttcgttatg agtgcagccc tggaaaggtt atgaagattc
     3241 agaaggggaa tgtttctggg gtagatattt atactactga gattcgggat tgggtaaagc
     3301 gaatggaaga tgagtttaaa actacacctt tagcaaggag agtaatgaag aatcaatctc
     3361 aagtagaaga attgatagat aaagtggatt cttattttgt agagaatggt ttggattcta
     3421 atgacaagtt ttctcatccg gaaatggagg aaatggaaag caaattagaa gattttaaag
     3481 ctttaattga agagagattg gctgaacaaa ttacagaaaa agatttttta aaacaagaag
     3541 tttcgaaact ttttaaggaa atagatttcc ttaaaaagca agtacagata ttaactaaaa
     3601 agaattggtt taaagcattt tgtagtaaaa ctggtaagtt tataagaaat aatcctgaga
     3661 ctacaaaagc gattgcccat acagctaaag aatgtcttcc ggaatctatt aaaatgagta
     3721 ttccagaagg ggctactgaa gtaatgaaca ctttgctgga agaacaaaag gttgaaaatt
     3781 agaatttgca ttagaaatat ttaatggtaa taggactctt gtttatattt attatgcaag
     3841 agtcctattt ttttattgtt aaagaaaagg attaattata ttacttgata tattagaaac
     3901 atctcggggc gcgaagtggg tgatgttatt agaaaatact ttggtatcaa tggtctgtga
     3961 tgttttttta ttgatgtttc taatatttat aactagggaa aagagggata agtatgtcta
     4021 gagaacgggt gaaaataaaa tttaagtctg aagatataaa agcggagatt gaaggttact
     4081 ctgatatgcc ttattggaat catgatttga aactggaatt tattttgaga cttttaaata
     4141 cagttgatga aaaacaattt cttgaaagtc agaatcacga tcagttggat tattacacta
     4201 aagaaatgat taaaatgatt gaagctagtt tagggtttga tggattatat cggttggttt
     4261 atggagatga attataggtt gggagaactc tttatgagtt cttttatatt cggattttat
     4321 gttattttaa aacaaagtta tttacaaaac gtcatacgaa atgataacat gttgttactg
     4381 ggttatttgg aggtgtttta atgagtaaag ctaattatgt gactgttggt aggaaaccga
     4441 aagacggtaa gaatccgaaa atccctgaga cttttagatt gccaaaagaa acatctgaaa
     4501 ggattaaggg attggttagt agtggacgtt tttctacgaa agcggaaatt gcagaaaaag
     4561 catttgagtt ttatttttct tatttggaaa aagaaaatgg gaagtaaatg tgttttggaa
     4621 tgatttttta gttgagggag attaactctc gaaaaaagtt acctgttatt ctaataagca
     4681 ggtaaagttc gagaatttct cgattattat ccaactacgc tttggacggg ttcaaaaata
     4741 taactgtttg ggggtttagg attttgttat gaatgatgta ctaaaatcta atttgttaga
     4801 tataacgatt gataaattaa gtattgttgc agattttaaa gaaggtatca aaaaagaaga
     4861 atttataaga atggttacaa gttcaaatat gccatattca ttgcaggcaa accgttcgaa
     4921 aaatttcggt tatgaggaag ttttaaagtg tgactctgtt gattgtggtt atattgagtt
     4981 ggcgggagaa ttaaaaaaag cgtcagttga tcttcctaaa ttaatctctc ataggattag
     5041 actagaaatt caaatagagc aggagaagaa agcaagagaa gttggagaat cgttactttc
     5101 taatgctgag tatcaaggtt tgtttgaaag tttagctgat gtgaaagaat tgatagctga
     5161 aactgatgaa gatggccgtt taactgatga aaggaaattg aaacggcacg agagacaaat
     5221 agaactgact attgagaagt tgaaaggcga aatagtcgca ttgaaatctg aaatagatga
     5281 gcaagaactt gcaactggtt ttaaaagtga agccatggaa aaaattcatg aggaacatgt
     5341 tcagatacta cttaatactt tggaaaataa agagaaattg attgaagtta aaagagatga
     5401 gttgttaaga aatgctgaaa tgtttttaca gttagatgca aatggttttc ttccgaataa
     5461 cggaaaaaag aaaaatgatc gtttgttgaa agatgttaga tttgaattta atcctaagca
     5521 tatggcatat aatcgtgtgg ttgatgaggc gatacgatta gtgttgggat tgttagaaaa
     5581 tattgaagtt acaggtatac atatagcttg tgattatcca gttaaaattg cagatttaaa
     5641 aataaaagat ttgtctagta aatcagaatg tatttatttg gatagagata aaaaaataga
     5701 aacgatgtac attggtaaac gaggttcgga taatcatctt tgtatctata ataaaaaaag
     5761 agagaatgaa gaaaatgaaa ctttagatca gtatcctgat gttgaacatg taacacgttt
     5821 tgaagctcgt ttgcgtaaga agaaagcgaa agaatggata gattcggaat ataatccgtt
     5881 tgattcgata attgttggag atttagaaaa agtagataac agtaaaatta aggtgaacga
     5941 tcgtattgtt ttagaggcta tcatgaatga ttatcatggt cgttattttg ggatgatgga
     6001 taaggatcaa aagagtaatt ggagaaagaa aataaaacaa catgttccta agttgtttga
     6061 tgttgctggg gattatgaaa agaaaaaagt tctactaact ggagaattag cagaactcat
     6121 gaaaaataaa cctagtaagc aaaaataaaa gttgaattta aaaagatata cttacaatcc
     6181 tttcgagtgt tggcgcactt ggaaggattt ttttatggtt tagaacttaa atttagtttt
     6241 gagaataact acgtctatta ttgcatgtat accgtatatt attcatttta atgactttta
     6301 tattgattgt catcaaagaa aaataaccca aaaagggtgc ggttttttat atatataaaa
     6361 catataaccc taccgttcat aaattatata tatttttatg aacgtttgca aatgatttta
     6421 gggacttttt gagacacttg tttttggctt tatgatgcgt ttatcagtta tttgagacta
     6481 ctttttgata cggttacttt tggaactgtc tcttttgtat tgcgttaatt ttagggcttc
     6541 attgatgcta tgatggtgta atgggctaat attaaggtga ttactggggg cttttatgtt
     6601 gagtgaacaa gtcatatatt attggaatga tgaattacag tattattatt tagctgaaaa
     6661 aaagaatggc agctataaat ttgtacaacc gttaaaaaaa ttagaattag atgatattag
     6721 tgatccgtta gaaagagaac aaatgaaaaa aatgctagag ataaaaaaac ggatgtcaga
     6781 aacaaaaggt aattggtttt aaaaaaagaa agacaaaatg tctttctttt tttaaaactt
     6841 taatttcgtg aatttgtgaa ttctttttta aatttataag ctatatagtt tatatcctaa
     6901 aacttcccgt tactaccgta caggatggaa aactaaagtg tgcgcatgaa tttttttagt
     6961 tatatatata acgataaaat accgtacgtt ttttgaaggt ttattttttg ggggagattt
     7021 tgatgtggaa tttgattttg aattttgttg aatttgaaat ctcgattttt cgattttcga
     7081 aatttttgtt ttctgttttc tttgtaattc cgtattccga aattacggag tttatatagt
     7141 atctctatga tgaactgaaa acgaaaaaac tttgctcgca ttttcatttc atagatatat
     7201 atatatcatt aaaaagtcgc accgttttaa tcgagttgtg tgataaaaaa agatgtttag
     7261 attatgtttg aatttgttgt tttgtgaatt ttttatttcg tgatttctgt atatttttaa
     7321 tcctttttac ccttaaagtc tgtttgtttt tgttcgcaaa tgaaaaaaac aaacagtcac
     7381 gcataacttt tattaaatat ataaatatat tattaaaata tctaaccctt ttgtggtgaa
     7441 aaaatataaa tagttaaagg aaaaaagact gataaaaata tcagtctttt ttcttgtttt
     7501 tagttataga gagtatcttt ttatggtatt ttctactttt tctctccagt tttctatatc
     7561 gtcaattgat attccgatct ttattaattc gcttggaatg aaactgttat ctaaaatcca
     7621 taaagtgagc ctttctaatg tgattctttc gtttccaatc gaataaatac gttcactttg
     7681 tttgcaggta tatggttttt taccaacata tttttctaca taaaagtatc tttttccatt
     7741 tgctttgtaa tttgttagaa atgccatagt aatgctcctt ttaggtattt ttatggttag
     7801 ttaaagagta gttctgtaat gttttttcaa ctttttcata ccaatatttc acatcgttta
     7861 tggatactcc gatttctatt agttcttttg gtatgcggtt gttatctaat aaccacatag
     7921 cgattctctc taaagctatt ttttgattgc cgattgagta aatatttata tatctatagt
     7981 gttttgagta atatggttgt gttccaacgt attgagatac ataaaagtaa atgtttcctt
     8041 ttactttttt tgctgttaga tatgccaaat cgttcttcct ttcatgttca ttattctttt
     8101 gtttttgtct gtggttatat tttaacctta taaagttaaa atatcaatac atataaataa
     8161 atataacctt taaaaggttt atttttactt ataaattgca taaaattatc tttataagta
     8221 aaatagagtg tatgttgaat ataaggagta agaagaatga agttagtaaa taacattcgt
     8281 atgattatgg cccaaaaaaa tatagataat attgcggagt taataaggat aactggtgtg
     8341 agtcgtaatt ctattaataa gctttggcat aatgaaagtg tgtcgtcttt aagattagat
     8401 actttaatag cgatctgtga gaagttagat gttaaattat cggacttaat tgaatatatt
     8461 ccaggtgaca ttgagtcaaa ataatttagg ggattttatg tacttggata gtttttgtat
     8521 gctaaaggta tagttggcta atggtatttt gtggtgctat atggcgcact tcaacttctt
     8581 ttgttaacgc atgagtatgt tttgaattag ggatatggaa ttgtagactt gctaggtgcg
     8641 tcaacacctt ggaagatgag aggtactggc atacttcgtg attttgttga actatattta
     8701 taatcatagt agtgctgtta aatgaaatgg tggtaatgat gattattaaa aagagtccta
     8761 ttttcagggc ttttttttat gttttttgtt tattgaacaa gatagagcat tttttgaaaa
     8821 gcgaattgtt tattgttatg ttaagctaaa tttaacttgg tatgtgagat ttaaaaaaaa
     8881 tggttaaaag ctatttgtag gtgatcaaat ttaccaaaaa aaagagaagt tactatattg
     8941 tacaagggta atggacaagt gtgtagcgat gaggattgaa agctgtatat agtccaattg
     9001 tttatgtttt aaaggtaaaa acttgtgaat aagtaattaa ttataggggg aactattgtt
     9061 gaggtattca gatataaaag tggggaatat atattatgcg gatttaaatc caataaggaa
     9121 atatgagttt ggagataatc atttaagtat aattttaagt aaaggtaaag ataaaaggac
     9181 agtgactatt
//
