LOCUS       NZ_CP068726             9518 bp    DNA     circular CON 11-MAY-2024
DEFINITION  Bacillus cereus strain AFA01 plasmid p2, complete sequence.
ACCESSION   NZ_CP068726
VERSION     NZ_CP068726.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17369857
            Assembly: GCF_016774575.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Lu,J. and Chen,H.
  TITLE     Characterization of Bacillus cereus AFA01, capable of degrading
            gluten and immunotoxic peptides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Lu,J. and Chen,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2021) State-Key Laboratory of Food Science and
            Technology, Nanchang University, No. 235, Nanjing East Road,
            Nanchang 330047, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.9%x
            Sequencing Technology :: PacBio RS II Single MoleculeReal Time
            (SMRT) and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016774575.1-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 05:11:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,006
            CDSs (total)                      :: 5,853
            Genes (coding)                    :: 5,687
            CDSs (with protein)               :: 5,687
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 65 of 166
            Pseudo Genes (incomplete)         :: 117 of 166
            Pseudo Genes (internal stop)      :: 41 of 166
            Pseudo Genes (multiple problems)  :: 47 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068726.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="AFA01"
                     /db_xref="taxon:1396"
                     /plasmid="p2"
                     /geo_loc_name="China: Nanchang"
                     /collection_date="2017-11"
     gene            complement(join(9168..9518,1..102))
                     /locus_tag="JM957_RS29610"
     CDS             complement(join(9168..9518,1..102))
                     /locus_tag="JM957_RS29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016107572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="WP_050267879.1"
                     /translation="MKKMLIFSIIVVCTMILFFLFQKEKFTNDKEVVKSQMSENESENY
                     YSKQLTLDQLQTALQNKEEKIVYFYKPNCPYCEEVSPLIVPMAKKENLDLEVLNLDSYK
                     EGWSHYKIEGTPTIVHYKGGKEVNRIVGATDENGYLEWFRSLKKTI"
                     /name="thioredoxin family protein"
     gene            complement(150..455)
                     /locus_tag="JM957_RS29615"
     CDS             complement(150..455)
                     /locus_tag="JM957_RS29615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050267881.1"
                     /translation="MENLTFVYFQPILISIVVLLILLSLFLLLKKNIDKSSKISCIIFS
                     VIALYFSGLLIIIEGTIIDELNLKGDQKSFYLSIFGIGISIINLIIAVMKKNGVKH"
                     /name="hypothetical protein"
     gene            complement(541..1449)
                     /locus_tag="JM957_RS29620"
     CDS             complement(541..1449)
                     /locus_tag="JM957_RS29620"
                     /inference="COORDINATES: protein motif:HMM:NF022240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2599 domain-containing protein"
                     /protein_id="WP_050267883.1"
                     /translation="MKSKIMKLIVVGSMTGTIFGVANNVSAQSNNVLDSNQYKNVLNQN
                     MNSEPKYLSFSTAEGETIKIGAMKSSDSKYGNSEIYKFNSSSNEKEIAPKIKKIENGVE
                     QIIEIEPLNANTDYNIPFDFQNGESIKLEKDGSAVIYNKTKDSIAVIMPTEAKTKSGKT
                     LEVKTKLINKNTLSYHVNFKGETEPVTIAAKALSYSFSQYFSSGKWITRDGMLSLSLQP
                     TSLLTKVNSNPNVNGAIRTDSWNKVKNKFSGSGNWKNTGSMENQYYCHVDTAQRFKTPW
                     NLEPHRPNVGYTQTVKKLCNP"
                     /name="DUF2599 domain-containing protein"
     gene            2354..3820
                     /locus_tag="JM957_RS29625"
     CDS             2354..3820
                     /locus_tag="JM957_RS29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901551.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_050267870.1"
                     /translation="MLGSISFNQSHQSSLSHNNRENIHGNPGIDPARSEENIYFVQKDI
                     RSVYKDVFQEAVDKYNEKQKRNDRKIENYYDKIHKNDKTHEQRELVVAIGEGKDDPKYR
                     VAKKEALKRYAEAFQERNPNLAVYNMVLHDDEANPHLHINYVPNFESSRGLTRRVGMDR
                     ALQQQGVEGTGRKLIAHWRELETAYIEQLAKEHIPNFERANVGSHKYMKVRQYKEYAEA
                     KSTIENQVQEKETELQTIDHDLKNVEEKTKKLQVMKNSLESDVIDKQKELETVKQQVES
                     ESEKLQLIGQRQVELEKRVEQMQKELDSATDRVPNEPVKIPFLRKEVVVEVQDKMFGKA
                     EITKKQTRNYVLSPEQYQEVTKQVNAAVTIKKDYECLRETDFVKEYESLKMTAEGWMKE
                     NRTLKQEKKQLQKEVGVLNREISSLKAHIKGLQTNIRVLYVQTKKVFKEQFKAFREIVK
                     NELDSKGIDNQFEREHKREISRHRDFDRER"
                     /name="plasmid recombination protein"
     gene            4030..4683
                     /locus_tag="JM957_RS29630"
     CDS             4030..4683
                     /locus_tag="JM957_RS29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016136977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050267873.1"
                     /translation="MTKVVDFGQAEKKAKIRDSKIDSIYDQLQAGGYSEEEKAMLLQLL
                     SKTTGGDEYFIGKKKKPTDRVRFVQLIMDNVNYLTEIEYLSSKEEAFLFKLAPYVEFKT
                     NVIIQKIDKDNVDTTTPASPTYLAERFKMARKNVSLTMNGLFKKGVLGVAAAGITTEDG
                     RACTSRTWFVNPNIMCCSPKDGIDKATQHIFRNSLRNFKIDESKKKYKLPIYLF"
                     /name="hypothetical protein"
     gene            complement(5295..5501)
                     /locus_tag="JM957_RS29635"
     CDS             complement(5295..5501)
                     /locus_tag="JM957_RS29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002084334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057598022.1"
                     /translation="MKSFGMLVCSTIFSVLLVYYNIHSFYNKFTSGNTYYWVNGILAAG
                     FLISLIINIKDIIKKNYTTSETN"
                     /name="hypothetical protein"
     gene            5851..>6078
                     /locus_tag="JM957_RS29640"
                     /pseudo=""
     CDS             5851..>6078
                     /locus_tag="JM957_RS29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020600.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(6331..6708)
                     /gene="qacH"
                     /locus_tag="JM957_RS29645"
     ARG             complement(6335..6708)
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(6708..7259)
                     /locus_tag="JM957_RS29650"
     CDS             complement(6708..7259)
                     /locus_tag="JM957_RS29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            7525..7833
                     /locus_tag="JM957_RS29655"
     CDS             7525..7833
                     /locus_tag="JM957_RS29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002035461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_072770324.1"
                     /translation="MLVLMIMLPISLVPVCYFFGYYGFIALLVLSILLMFASLKVESIK
                     REHNLKTYREIVDFVEGKPVNKVKVDKKNRVLKTSLILLIPLISYIVVYLGMTAFGV"
                     /name="transcriptional regulator"
     gene            8289..8849
                     /locus_tag="JM957_RS29660"
     CDS             8289..8849
                     /locus_tag="JM957_RS29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421195.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_050267878.1"
                     /translation="MELKSEMHTPKIVADLLGITPDLLKVWSNEFNIQTERSQGGHRRY
                     SKENIEELKAIKEKIQAQKWSYDQVRSWRNGELDLFVSKEEKSELEKKLNEVLENQQLQ
                     NQFNQALVQKLQEITNELVTTKEHLANTEKKLTEVEGKNSELEVFIEKKLEKRDQLLLE
                     NIRDMQAQNQKQKRKGFFGLFKK"
                     /name="MerR family transcriptional regulator"
     ARG             complement(6332..6708)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
     Typing          4031..4683
                     /name="rep_cluster_1832"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.118"
                     /coverage="100.0"
                     /evalue="1.68e-143"
     Typing          2355..3817
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.959"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttactact tctttgtcat ttgtaaattt ctctttttga aacagaaaaa aaagtatcat
       61 tgtacaaaca acaataatac taaaaattaa cattttcttc attttttcac ctcaagaaaa
      121 aagattgtat ttaagagtaa caattcctat taatgtttta ctccgttttt tttcattact
      181 gctataatta aattaataat agaaattcca ataccgaaaa tagataaata gaaagatttc
      241 tggtcacctt taagattgag ttcgtctata attgttccct caataataat taaaagtcca
      301 ctgaaatata gagcgataac agaaaaaatg atgcaagaaa tctttgaact tttatcaata
      361 ttctttttca ataataagaa caatgatagt aaaattaata agacaacaat tgagattaat
      421 ataggttgaa aatatacaaa tgttaaattt tccaaattaa ttcctccaat taaatattat
      481 taacaaatat aaaacaagaa agtataacta tgtatagtta tactttccag cagttaaagt
      541 ttatggatta caaagttttt ttacagtttg cgtataacct acatttggac gatgtggttc
      601 taaattccaa ggtgttttaa atctttgagc agtatccaca tgacaataat actgattttc
      661 catggatcct gtatttttcc aattaccact tcctgagaat ttattcttta ctttattcca
      721 actgtctgtt cgaatagcac catttacatt tggattacta tttacttttg ttaaaagtga
      781 tgtaggttgt aaacttaagg aaagcatacc gtctcgtgta atccatttac cactagaaaa
      841 atattgagaa aaagaataag atagagcttt tgctgcaatt gttacgggct cagtttctcc
      901 tttgaaattc acatgatatg ataatgtatt tttatttata agtttagttt ttacttctag
      961 tgttttacct gattttgttt ttgcttctgt aggcattata acagcaatag aatctttagt
     1021 tttattatag ataactgcag agccatcttt ttctaatttt atagactctc cattctgaaa
     1081 atcaaatgga atattataat ctgtattagc atttaacggt tcaatttcga ttatttgctc
     1141 aacaccattc tcaattttct tgatttttgg tgctatttct ttttcatttg aggagctatt
     1201 aaatttataa atctctgaat ttccatattt agaatcagaa cttttcatag cgccaatctt
     1261 aattgtttct ccttcagcag tagaaaaaga taaatattta ggttcagaat tcatattttg
     1321 atttaataca tttttatatt gattactatc taatacatta tttgattgcg ccgacacatt
     1381 gtttgcaaca ccaaaaatag ttcctgtcat agatcctact acaatcaatt tcatgatttt
     1441 acttttcaat ttaatgcctc cccatttttt ataagattta ttgccaatta ttaggttacc
     1501 atattttttg aataattgga atacattgta atataaattg acatattttt gggaaataca
     1561 tatcgattta tattacatat tagaaaatat gacctgttcc ttgcggaatc tttagctatt
     1621 tctgttcaat tactacacaa atttttaata tagataattc ttttttgttt aataagttta
     1681 aagttaaagc ttttatatgt tttgaatttt gaagggtttt acataggagg ggaatttgat
     1741 ttttagtttt taatcccctt atttatagcg ttttgtatgt atacttttca tgttcatttt
     1801 gtgagggtat tagggaattt ggtgagaagt caatgattaa gaggatttga atgtaaacta
     1861 atattgaaaa gtttactttc gatagaatga gaaagagtcc tattaattta ggactttttt
     1921 tcgttttagg gtaataaaga gagcaaagtg aaagtgatca gtagaggtcg ttgcgtgtgt
     1981 tggtcaatca aaacggacct agcgaagcga agggagtatt tgacttgacc aactagacta
     2041 cacccgaacc ctcttggtat tgaacaaacg gccgtttgtt cctgccaacc ggcgagggag
     2101 cccctcaagg attgaggggt tggggagttc gattgatggg gtctggggaa gagttcccca
     2161 gtgggtttgg gcaaagccca aggtttgttt ttgccatgct aaacatggct cggcaacgcc
     2221 gaaaagcgtc gcagacccct atcgattttg atgcgtagcg tcaaaatacg tatagggtta
     2281 cgttaattga caccatttcg tcgctttgct agacttatgg tatcaattaa tctagggaaa
     2341 gtggtggcac tttatgttgg gttctatctc atttaatcaa tctcaccaaa gttcattaag
     2401 ccacaataat agggaaaata ttcatgggaa tcctggcatt gatccagcta ggtcagagga
     2461 gaatatttac tttgttcaaa aggacatccg aagtgtatac aaggatgtct ttcaagaagc
     2521 ggtggacaag tataacgaga agcaaaaacg gaatgaccgt aagattgaga attactatga
     2581 caaaatacat aaaaatgata agacccatga gcaacgagaa ttggtcgtag cgattggaga
     2641 aggtaaagat gacccaaagt atagggtagc taagaaagaa gcgttaaaac ggtatgctga
     2701 agcgtttcaa gagcggaatc caaacctagc ggtttataac atggttttac atgatgatga
     2761 agcgaatccg catttgcata ttaactatgt accaaatttc gaaagtagtc gaggattaac
     2821 gaggcgtgtc ggaatggata gagctttgca gcaacaaggt gtagaaggaa cgggaagaaa
     2881 gttaattgca cattggagag aattagaaac ggcctatatt gagcaattgg ctaaagaaca
     2941 cattccgaat tttgaacgtg ctaatgtagg ttcacataag tacatgaaag tccgtcaata
     3001 caaggaatat gcagaagcga agtcaactat tgaaaatcaa gtacaagaaa aagaaacaga
     3061 attacaaacg attgatcatg atttgaaaaa cgttgaagaa aagacaaaaa aattacaagt
     3121 aatgaaaaat agtttggaaa gtgatgtaat tgataaacaa aaagaattag agacagtgaa
     3181 acaacaagta gagagtgaaa gtgaaaaatt gcagttaatt ggccagcgtc aggtagagtt
     3241 agaaaaaaga gtagaacaaa tgcaaaaaga attagatagt gctacggatc gagtgccaaa
     3301 tgaacctgtt aaaattccat ttttacgtaa agaagtagta gtagaagtac aggataaaat
     3361 gtttggaaaa gctgaaatta caaagaaaca gacaagaaat tatgtgttat caccagaaca
     3421 atatcaagaa gtaacaaagc aagtgaatgc agcggtcact atcaaaaagg attatgagtg
     3481 tttgagagaa acagactttg tgaaagagta tgagagttta aaaatgactg cagaaggttg
     3541 gatgaaagaa aatagaacat tgaaacagga aaaaaaacag ttgcaaaaag aagttggcgt
     3601 attaaataga gaaattagct cattaaaagc tcatataaag ggtttgcaga cgaatataag
     3661 agttttgtat gtacaaacga aaaaagtttt taaagagcaa tttaaggcgt ttagagaaat
     3721 tgttaaaaat gaattggata gtaagggaat agataatcaa tttgaacgtg aacataagag
     3781 agaaataagc agacatcgag attttgatag ggaaagatag tattgaatgt tgtatttttg
     3841 caagagacgg aaagctttaa caaggtgttt catgttatta tagagaaaga catacaaacg
     3901 agaaaaaccg cccaagcttt ggacggcaag gcggtttttc ataataaggt tgggatcaac
     3961 caatatatat tctatataca ttctaacata taaaagttga ttccttccac taagggagag
     4021 aattatttta tgacaaaagt tgtagatttt ggacaagctg aaaaaaaggc gaaaataaga
     4081 gatagtaaga ttgatagtat ttatgaccag ttgcaagccg gtggttactc agaagaagag
     4141 aaagcaatgc tcttgcagtt attaagtaaa acaactggtg gagatgagta tttcataggg
     4201 aaaaagaaaa aacctacaga tcgggtgagg tttgtgcagc taattatgga taacgttaat
     4261 tatcttactg aaattgagta tttgagtagc aaggaagaag catttttatt taaacttgct
     4321 ccatatgttg aatttaaaac aaatgttatt attcaaaaaa ttgataagga caatgtagat
     4381 acaactactc cagcatcacc tacatattta gccgaacgtt ttaaaatggc aagaaaaaat
     4441 gtttctttaa caatgaatgg tttatttaaa aaaggggttt taggagttgc cgcagctgga
     4501 ataacaacag aagatggtcg tgcatgtaca agtcgtacat ggtttgtaaa tcctaatatt
     4561 atgtgttgta gtcctaaaga tggaatagat aaagcgactc aacatatttt tagaaattca
     4621 ttaaggaact ttaaaattga tgagtctaag aaaaaatata aattaccaat ctatttattt
     4681 tagatgtcaa attctcattg acatcttttt tttattatgt ttttcctatt attaggtgtt
     4741 acgaaaaagt aacacaggtg ttacgaaaaa gtaacacctc atatgctata aacccttggt
     4801 attactggct ttttttattt gctctatctc actcttatat atagggctaa aagttccgca
     4861 aggaacaggt agtatttttg agcaaacaac gctcaaaaat ctccaccttc aggagaaaaa
     4921 aagtcggttc gttaatcctc acctaaaggc tatcgccttt tctttttttc tctgtccttg
     4981 cagaactttt ttcggctcgt gtgcttttct cgccaagccg acagacaaaa agcaaagtaa
     5041 tggttctcga cttggactag caagtataca agccgaaccg cttagatcat tcgtccaaaa
     5101 aataatcttt attcaaaaat agatacgtaa tacgtggggt ttcgccccca cacgacgagc
     5161 cagcactcta gccaaaaagc aatggaagac caaagggcaa gatccaaaaa aaaggaatag
     5221 gcacgctaag ggtttggcat gtcaaacctt aaaaactatg tttttgaata gaaaaagaag
     5281 ccataatagg cttcttaatt agtttcagaa gttgtgtaat tttttttgat gatatctttt
     5341 atatttataa ttaaggatat aaggaatccg gcagctagaa taccgtttac ccaataatat
     5401 gtattccctg atgtaaattt attataaaag gaatggatat tataatatac tagaagcact
     5461 gaaaagatag tagagcatac taacatacca aaacttttca tactgttcac ctcaattcta
     5521 aaattggtaa cttttgttat aattatacat gtaaataaag agattttaag caaaacatac
     5581 aataagctgt ccagatggac agcttattta cataactacc gttgtaggaa gtaaaagtaa
     5641 acttttcaca tccaaaaaag ttttattttt taaatagttg cttaaatttg ggtcagcaat
     5701 tgtattagct gatccagcac ttatacctaa aaataaaggt aaaatagaat gataaaacat
     5761 ttttacactg tagaatcatg ctattataaa gaaatttggt agtctttaat tagcctttaa
     5821 cttaaccttg aacttaaggg agatgtatag atgaacatta gtaaacagat acaatatttt
     5881 cgtaaacggg ataatatgtc ccaagaagag ctagcagaaa aacttcatgt ttccagacaa
     5941 tctatttcta actgggaaaa tgaaagaaat tatccagata ttcataattt attaatgatg
     6001 agtattctct ttgatgtctc cctagatgat ttagttaaag gagatgtaaa aatcatgaaa
     6061 gaaaaacttc aaaaatcaat ttttcttaag tggggtagcg tcagggaaat gcggatttac
     6121 aacggtaagc atttgataca agtcttatga gacagtcaga agtcatttaa aacgctaatt
     6181 tcattatgtc tcaataaact ctactctatt ggaattgtga agtgatacac ttaaactaac
     6241 gtacgcaggt ttgttgaata aggacttcaa aaaataccct taattaagct aaaaagtcct
     6301 tctctttatt aagaaggact tttttatgtg ttattccatt gtctcattca ttgtaatagt
     6361 ttcgcttgct tcatgaccag attctccatg tcctggtcca aacaaattca atattacaac
     6421 tcctacaaga atcagtgtta ttcctgcaat gctagccatg ttaagttttt ccttccaaac
     6481 taaaactgaa attactgtag tcaaaacaag tcctaaacct gcccataatg catatgcagt
     6541 gtttaagggg attgttttta gcgacaagga taaaaagaaa aaacaaccaa cgaaagctat
     6601 tattacccct attgtaggat agagttttga aaagccttca gatgctttta atagggaaga
     6661 gccaataatc tcccccacta ttgcaagtgc taaatataga tatgacatta ttcgtcctcc
     6721 tgaatcatgt ttattagttc atcaaaaact tgtgttttta gttcttcatt taatggtgcc
     6781 attccaaata tttctgaaaa ccaaagacca tctatggata atcttgtaat tgttattttg
     6841 acaggatcaa ttccatcagt tattaacttt gtatgcaaaa tatcgtatct ctgccggaat
     6901 tcatctaata aatctggatt aataaacata gtagccatta gtgcggaatt tagattattg
     6961 ttttctaaat cggaaaatgt agatttaaga taagccctat tccattttcc tattgcgtta
     7021 tcatcgtcgt tattaactaa cgtattaatg cattcgaaat aattatttgt ccaatcttcc
     7081 accattcctt taattaatgc ttccttgcta ggaaaatggt ataataatcc acctttactc
     7141 acgccagctc tttgggcgac tgcctctaat gtgagtttga caactccttc ttcttttaca
     7201 atttcagatg cagcaatcaa tatatttttt ttcttattag catgctttga aatagacata
     7261 ataacctcca taacttagat acatataata taccgtctgg acggtttttt tgtcaatcga
     7321 attaaagctc ttatttaata tcactctttc acataaaaat aatccaataa attaatctaa
     7381 agattgtgaa gaaatgtact taaagtaaag ggagctttag ttcaacagtc gatttcttaa
     7441 aatcggcttt atttatgggt gaaaattaca ttaccgttgt aaatccgcat tttcaggact
     7501 ctacccctta agtggagtta tatcatgtta gtattaatga ttatgcttcc tatttctctt
     7561 gtacctgtat gttacttttt tggttattat ggttttatag cccttttggt actttctatt
     7621 cttcttatgt ttgcatcatt gaaagttgaa agtataaaaa gagagcataa tttaaaaact
     7681 tatcgtgaaa tagttgattt tgtagaagga aaacctgtaa ataaagtaaa ggttgataaa
     7741 aaaaatagag tattgaaaac aagtttgatt cttttaattc ctttaatcag ttatattgta
     7801 gtttatttag gaatgactgc gtttggagta taatttagaa aatattgatt tgattttttc
     7861 tgatgagagt aacattttgt tactctcttt aatttttatt gtgaataaat attcttatta
     7921 agttaacata atgtctcatt atcggtagca actattttga tagaatcctt gtatttacgg
     7981 ggattcttct ttttttgtct gtttatttgg tatacaggat tttatacatc gttgatgtag
     8041 cggggattct agggattcgc actcttcttt ttttgtataa aatcttgtat atagaaaaaa
     8101 gattttgatg ttattatcga gaagtaatac atatcggtgg taaaatcgga atgtatttcg
     8161 gaaattacat cgtttttaaa atcggttttc atatcggttg taagtacatt ccgattttga
     8221 ttggaatgtt ggtttaatcg tgttttttaa gtaaaaaacg tattccgttt tttggggagg
     8281 aataaaaatt ggaactaaaa tcggaaatgc acacaccgaa aattgttgcg gatcttttag
     8341 gaatcacacc cgatttgtta aaggtatggt caaatgaatt taatatacaa acggagcgaa
     8401 gtcagggagg acatcgtaga tactcgaagg aaaatataga agaattaaaa gcaataaaag
     8461 aaaaaataca ggcccaaaaa tggtcttacg atcaagttcg ttcatggcga aacggagaac
     8521 ttgatttgtt tgtttcaaaa gaagaaaaat cagaattaga aaagaaactg aatgaagtat
     8581 tagaaaatca acagttacaa aatcaattta atcaagcact cgttcagaaa ctacaagaaa
     8641 ttacgaatga attggttacg acaaaagaac atttagcgaa tacggagaaa aagttaactg
     8701 aagtagaagg gaaaaacagt gaattagaag tttttataga gaagaagcta gagaaacgag
     8761 atcagttatt gcttgaaaac attagagata tgcaggcaca aaatcagaaa caaaaacgga
     8821 aaggtttttt tggtttgttt aaaaagtaaa tttggctggt attttatgta gggttctaat
     8881 gtcggaaaaa gaagaataga aatatagcat acatacaata agggaatgca gtatagatca
     8941 gtctacaagc attcccttgc tataaagatg ttaataatat tttaatgttg ttactgctat
     9001 atatgtcata ctaattaaga ttaatatatc tagtgttaaa aaaacatatg atattatttt
     9061 caatcatata tttaatatat agtattgtag ttgagtgaat atttcatgct atgattccta
     9121 tacttggagt tcttttttta gaatcctaga atagttatta tttatattta aattgttttt
     9181 tttaaggaac gaaaccattc taaatagcca ttttcatctg tagcacctac tattctattc
     9241 acttcttttc cacccttata atggactatt gttggtgttc cttctatttt gtaatgactc
     9301 catccctctt tatatgaatc aagattgagt acttctaaat ctaaattttc ttttttcgcc
     9361 ataggaacta taagaggaga aacttcttca caataaggac aatttggttt gtaaaaatat
     9421 acaatttttt cttccttgtt ttgtaatgcc gtttgtaatt gatctaatgt taattgtttt
     9481 gaataataat tttcactttc attctctgac atttggga
//
