LOCUS       NZ_CP068693            10206 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain RJBSI76 plasmid
            pRJBSI76-4, complete sequence.
ACCESSION   NZ_CP068693
VERSION     NZ_CP068693.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17349264
            Assembly: GCF_016774435.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Qu,J. and Ou,H.-Y.
  TITLE     A clinical bloodstream infection-causing ST11 carbapenem-resistant
            Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   Qu,J. and Ou,H.-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2021) Department of Pulmonary and Critical Care
            Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of
            Medicine, No. 197 Ruijin Er Road, Shanghai 200025, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 06-MAY-2020
            Assembly Method                   :: SPAdes v. v3.14
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 490.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:33:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,166
            CDSs (total)                      :: 6,051
            Genes (coding)                    :: 5,869
            CDSs (with protein)               :: 5,869
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 70 of 182
            Pseudo Genes (incomplete)         :: 123 of 182
            Pseudo Genes (internal stop)      :: 23 of 182
            Pseudo Genes (multiple problems)  :: 33 of 182
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068693.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="RJBSI76"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pRJBSI76-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="31.12 N 121.27 E"
                     /collection_date="2018-12-16"
                     /collected_by="Department of Pulmonary and Critical Care
                     Medicine, Ruijin Hospital Affiliated with Shanghai Jiao
                     Tong University"
     gene            352..597
                     /locus_tag="I5002_RS30555"
                     /old_locus_tag="I5002_30555"
     CDS             352..597
                     /locus_tag="I5002_RS30555"
                     /old_locus_tag="I5002_30555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            871..1242
                     /gene="tolA"
                     /locus_tag="I5002_RS30560"
                     /old_locus_tag="I5002_30560"
     CDS             871..1242
                     /gene="tolA"
                     /locus_tag="I5002_RS30560"
                     /old_locus_tag="I5002_30560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1239..1604
                     /locus_tag="I5002_RS31010"
     CDS             1239..1604
                     /locus_tag="I5002_RS31010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1779..2057
                     /locus_tag="I5002_RS30570"
                     /old_locus_tag="I5002_30570"
     CDS             1779..2057
                     /locus_tag="I5002_RS30570"
                     /old_locus_tag="I5002_30570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2045..2359
                     /locus_tag="I5002_RS30575"
                     /old_locus_tag="I5002_30575"
     CDS             2045..2359
                     /locus_tag="I5002_RS30575"
                     /old_locus_tag="I5002_30575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2523..2951
                     /locus_tag="I5002_RS30580"
                     /old_locus_tag="I5002_30580"
     CDS             2523..2951
                     /locus_tag="I5002_RS30580"
                     /old_locus_tag="I5002_30580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2977..3156
                     /locus_tag="I5002_RS30585"
                     /old_locus_tag="I5002_30585"
     CDS             2977..3156
                     /locus_tag="I5002_RS30585"
                     /old_locus_tag="I5002_30585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3183..3713)
                     /locus_tag="I5002_RS30590"
                     /old_locus_tag="I5002_30590"
     CDS             complement(3183..3713)
                     /locus_tag="I5002_RS30590"
                     /old_locus_tag="I5002_30590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3720..4451)
                     /gene="mobC"
                     /locus_tag="I5002_RS30595"
                     /old_locus_tag="I5002_30595"
     CDS             complement(3720..4451)
                     /gene="mobC"
                     /locus_tag="I5002_RS30595"
                     /old_locus_tag="I5002_30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4451..6415)
                     /locus_tag="I5002_RS30600"
                     /old_locus_tag="I5002_30600"
     CDS             complement(4451..6415)
                     /locus_tag="I5002_RS30600"
                     /old_locus_tag="I5002_30600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7899..8048)
                     /locus_tag="I5002_RS30605"
                     /old_locus_tag="I5002_30605"
     CDS             complement(7899..8048)
                     /locus_tag="I5002_RS30605"
                     /old_locus_tag="I5002_30605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8133..8390)
                     /locus_tag="I5002_RS30610"
                     /old_locus_tag="I5002_30610"
     CDS             complement(8133..8390)
                     /locus_tag="I5002_RS30610"
                     /old_locus_tag="I5002_30610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8400..10085)
                     /locus_tag="I5002_RS30615"
                     /old_locus_tag="I5002_30615"
     CDS             complement(8400..10085)
                     /locus_tag="I5002_RS30615"
                     /old_locus_tag="I5002_30615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3400..10206
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7218..7796
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3724..4451)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cttgtggatt gctggtcctt caatggtcgg ggactttcac cccttgatct ctaaccggtc
       61 tcccggcgca cactgtacat atacacagta taattattac cttactcctc ccgttttcag
      121 gtgtcaatat atgagattag ttctcatctt caggcacaaa aaagcgcccg gagccacctg
      181 taagcagatc gcgtcttttc ccgaaaattt ctcatggcaa caaattttat tatttaagac
      241 attcatcaca cttacatttc agatggtata gacacatctg aaagctaact ggcatgagga
      301 gaggacctgc ggttaaactg ttcgggaaac ctgagacgcc cggaggccac tatgacgcac
      361 ctgaaactga ctcccgctga agaaaagcgc ctggcggacg cctttgtcgc tcaggcactg
      421 gccatgggcc tgaaagggaa caaaacgctg ccaaaggcgg taaaacaaca ggtgcgggaa
      481 agggtgcttc gtgccattca ggacgaacgt gaagccaggc gggcgcagga aatgcgtcgg
      541 gagaaatacg aggctacgga aaccaccttc acctggcagc cacagcgccg ccggtaaggg
      601 gtatttacca taacgtcgca ttgcccgcac cgctcagacg gtctgagggc gtttccggcg
      661 gctacgggat aaaacaggca acaaaccacc cggaaaaccg ccggaaacgc gcccggtaaa
      721 ttgtaacccg ctgcatgcct atgcacgcaa ctgcatggcg ggccgtttgg tgtaaaaacg
      781 atacgataag ccgtgtttat gtaaatgaac gtggccgtaa agagtgacaa atatacatgt
      841 aacatttttt actgacaggt catcaacccg atgcgtttaa taccctttat cctgctggca
      901 ttgtgcagcc ccttactcct gacggcctgt aagcattcca ccacccagcc gccgaaaatt
      961 tatactctgg ataagtcatc taaaatcagt agctacgctg cggccatcaa gaccgctgtt
     1021 gaaatgcaga tgtttgaacc agaggcctat gcgggaaaaa tgtgcaccgt gcggatatcc
     1081 atacaacgtg atggcactct gaacagcgcc attgctgaag gaggagatcc gaaactctgt
     1141 aaagcagcaa tatcagccgt tacccacgcc aaaattccgc cggcaccgga tgaaaatacc
     1201 tggcaaatat tcaaaaacgc ccctctggat ttcagaccat gaaagctttt actctgacag
     1261 tgctggcggc catgttcctc actccggtac atgcgatacc actaaaaccc gtaaccacac
     1321 tgcagccaac ctacccggta caggccgcag caaaacgcat cgaaggtcag gtgaccgcca
     1381 cgtttgatat tctggacgat ggacgagtgg acaacgtgtc gcttaccggc tgtcaccttt
     1441 tttttcggga aacgctgacg gcaatgaggc actggctata tcccgcaggg cacccggcaa
     1501 tgcgacaggt tataaatttt gaattcagtc tgaaaaatga cggcttacag gatccgcgac
     1561 cagtcaccac ccaaaagctc aatgatgatg gttgccgcca gtaacaggtc ctttaacccg
     1621 caggctgcaa tagtcctgga tgatgagtga gcctgtagca gccctgatta gtggaagcga
     1681 agctgaaaaa tgggcagcaa aagccgtatt tctggcaaat agcgccgcca cggattaaag
     1741 tagtcatgtg taatacatat gactacaaag gggcttttat gtcatcagca acatccatca
     1801 ggctcgatga agaacttaag gaccggctga aaacgctggc cgatgaccgg caccgctcag
     1861 cgcacgcgct aatgctggag gcaatcaccg aatacatcga ccgggaagag aagcgcagcc
     1921 agtacctgcg ggacggacag gccgcatggc agcactatca ggaaacgggt ctgcacctta
     1981 ctgcggaaga agcggaagcc tggataagta cctggggaac tgagaacgag caggatgctc
     2041 cgccatgcca caggtaacga tttccgcgct ggcacagcgc gatttacaac gccttcagga
     2101 ctttctcaaa accaaaaatc ggctggcagc cagaaaagga ggtgaggtga tcgtccgggc
     2161 tatccagcaa ctgaaaacat tgccagacat tggccgcccg gtgccgtttc tgccgctgga
     2221 atataaggaa ctggtgatcg ggtttggcga cagtggctat gtgatgctct accgccacga
     2281 ccgggaaatg gaccagattg tgatcgtcac cgtcaggcat cagaaagaat ccgggtatcc
     2341 gggggcagac agcctctaat aaccgatgac gggggcgcaa ttcattgcgt ggccgtcgcc
     2401 ggtttgacaa gcgcagcgcg tcagaaaacc acatcctgaa cggaaaaacg ggtaaaattc
     2461 agcgatacat ttaccgtgag aaattacaca cctttcacac ccgataaaat caggggtata
     2521 cagtggcgaa aacattgctc aacctttcac aggcagcaca agccgcgggc attaccagaa
     2581 gaacgctata caatcatgta aaacagggaa aggttaccgt gtcacgtgac gggaaaaaca
     2641 accctgtcgt ggatgtgtca gaactgatcc gtgtatacgg aaatgtgaat atccctgaga
     2701 aacaaattcc cggcatttca caccgtgaaa atacacaaaa aaatttccca caggaacagc
     2761 ttctggcaat gcaaaaggaa ctggccgatc tccggcaggc tgtcaccctg atgctggaag
     2821 ataaaacatc ccgggaagaa gaacgccgcc agcacgatga tgagcgccgg aaactccagg
     2881 ccgaagtgga cagattgaca acagaactga cacagaagaa aaaacgcttc tggtccggct
     2941 ggtttagctg agaacttacc gaagggaatg gagaacatga cgaagcagga aaaaactgcc
     3001 ctcaatatgg ctaaattcat taaggatcag tcgctgctgt tgctggaaaa gctgaacgag
     3061 ctggacatgg ataccccggc cgacatgtgc gaacggcttc acgaggatgc ggaaacactc
     3121 tggcaggtga tgcgggataa gctcggggaa gaataacagc accggccatg ccggtgctgg
     3181 cgttattttt tgccgaacgg ccaccaccgg cgggcgggtt tgtcctccgg cagttcaggc
     3241 accggcacgt taaaatagcg gctgttatat tcccgcatca gctccgcttt cagcttctcg
     3301 ttcaccgcgc tttccccgtg ccgctcgctg accacctcaa tcatcgcgtt aatctgctcc
     3361 ggtgagcact tcccggcatc aaccacctgc acaccgttct cgatggcgaa ccgctccacc
     3421 tttcgataga ggtaatcaca cagcttcatt cctgtgggtt tgtcccgttc ccattgcgga
     3481 ttttcaccgg tgaaaaagta cgtggctttt tcgtcgctgt tgagccgctc gtcatacatc
     3541 ccgatggatt tcatcatcgc cagatgctgg gcccggtcga caaaacagaa aacatacgcc
     3601 ccggtctgcc aggcgagcac caccatgtct tccagatgct cgctggttgg cataatgcag
     3661 cgctcgccgt ctttgtcttc cacgtcctca ataaatacca tgtacagcgc cataatccct
     3721 cactccgttg tcataaattt aaagcacgcg aacagcctct cccggtccag ctccaccgtt
     3781 ttgcgggcct cgccggtctg tggattcacg atttcatcca ccgccagaat cgcccgctgt
     3841 acccggacaa gcgtcggccg gtcagggcag atgtaccaga tttcatcgat gccgttcgcc
     3901 cggcggttga acagataccc ggacaccact tcctgatacc gcctgcggct cttgacggtg
     3961 cgctctacct caaatgccac cgttctgccg tccagctccg ccagcgcgtc cggcactttc
     4021 atccctttca gggccatacg gtgcaggtac cgccagccgg tggccccccg gccctccatg
     4081 gagaggcgca gggactgcac ggccagccgg tgcggcagct gcgccgccga cacccttgac
     4141 ggctgaaacg gaataacgtc cgtcagcggc tcgtcctcgt cgaacgagaa cgccaccccg
     4201 tgcggcgtca gcccccacac gctgacctga cggccgtcaa ccgggtattt cgcccggacc
     4261 agcagccctt tcgccaccag actgtccagg aagcggtaaa ccggctgtac cgccgcaatc
     4321 cccagccgtc gcgcaaccag ctccgccgtg gaacacagct cttcccgcag ccagcgcagg
     4381 gtcagccgtg ttttctccgc gttccgcgcc tgccgcttcg cgtggctgac gttgtactgc
     4441 tccagtgtca tcagtaaagc cccgcaaaat catcatccgc ttttggtccg ccgccgtcgg
     4501 ccggaggcag gtactccgcc agcgggtccg ttttcgccag caccgggaag gttttcagcg
     4561 aaatttcccg gcgctcaacc cgcagcgggc tgctgaacgc cagccgcgcc acgcccagcc
     4621 cggtcatcac cgcgaacccg tcaggcaggt gttgcagggt gttcacgtca aacagcggcc
     4681 gcgcctcctt ctggatgaag ctgtcgccgc tgatgtgctc cacgttcccc gcctcccggc
     4741 tggcccggcg gcgctccacg tccacccgga tctccccggt ctggcccgcc gcccactgcg
     4801 cggactcctg gctggccgcg cgatagaacc agcggatcgg cgtgttatcc agtaccgtgg
     4861 ttttcacaaa atcagcgggc agatcctgcc cgcactgacc gaagtcgccc agcgactggt
     4921 gcgcgagcag caggttgcag tcccggctgc gcagcgtccc cagcgcgttc agcacgtact
     4981 tcgacagcag atatttaatc tcatcgagca tgatgctcgc gtgcggccac ctccggaact
     5041 catcccgcgc aatgatgatt tgcgcgcagc gggcaaacag catcttctgc acccggatca
     5101 ccgcctcatc gtccatcgag ccgatgacgt acaggcagcc gccgccgttg attatccccg
     5161 ccacatcgat accgctgtcc gtctgcagga cgctcaggtc ggccacgttc tccagcttgg
     5221 tgatcagccc cttcacgtcc tttttcaggg cctccggcaa cgcgtacgcc gccgccagca
     5281 cctgctgaat gtttgccccc tgcgggaact gctccgcaat cagcttcgcc gctttctgct
     5341 cgctgatgcg gtagtggtcc gccacgtcgc cggtctcact caggttaaac ccggcaacca
     5401 gcagattctt cagggcgtac tggtcgagat cgcggaacag attcagctgc gccggtttcc
     5461 ccggccgcag atccagcagg gtgaagtccg ggcagtgcgc ccggaagacg tgcggcgccc
     5521 acgcgtcccc tttggggtcg aagtacacca cagcgtcccc gaaatcgcgt acgcagcgaa
     5581 tgagcgcgtt ggtggccatc gcgctcttgc cgctcccggg caggccgagg atctgaatat
     5641 tggtcttgcg ccaggtatca cgagggacca gcaccggctg gccctgctca tccttaccca
     5701 gaaacaccgc ctgctgtttc cgggcctgct ccgtggaaaa atcggccagc gcgttaaccg
     5761 gtaccggctc cccgaaatgc aggtggcgga cgtctgtcat ttctccggtc cgctggctgc
     5821 tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc gtcagcggcc gcaaaatgac
     5881 catcaagcag caggaacgcc agcagccgcg ccagaaggac gccggcgaca agccctccgg
     5941 cacaccaggc tatcccgaac aggttagccg gctgcagcag gtaataatct gcgttcgcat
     6001 agaggtccgg gcgcaggacg gtttctcccc agctctcgat aaacacggcg acaaccagcc
     6061 cccccagaag ggccagaccg gcagcgccgg tcagccagta cgcgtagttc cagacatgtc
     6121 gccgtgccct gaccatccgc ccgggtgaat ccagcgaggc cagcaccgaa aatggcacag
     6181 tccggttatt atcgcgctta tagcgatcgg catactcccg ccgcgcctta tggctcagcc
     6241 ccacaaaacg cattcggccg gcccagagtt cagccagacc gaaaaacagg ccgataacca
     6301 gaaaaaaacc ggccagactg taatactccc ggtacagcca gctgacccac gggtaaatct
     6361 gccgggccag ctccgggata tgccactgcc aggcaagcga atccgtatta aacatgcaat
     6421 ctctctcctt cagacgacgg gtttaccggc gtgatgcgca gcccccgctg cacaagccgc
     6481 ggacatgccc caaggcatgg cggcggcggt cacgtccgtg gtgattgcgt tcagctttca
     6541 ccgcggaggc cagcctcttt ccgccccatg cggaaaaggc gcagccccgg aggaacaacg
     6601 agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt tccggtgttc
     6661 cggcccccgt cagagttcgc catagcgagc ggctgaccac ccgcaaaatc aggagcagct
     6721 ttgctgcttc gtcaggtgcg tccagcaccg tggatttttg tgggtggtca ggcgtgagcc
     6781 tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca gccggacaga gccagaaaga
     6841 ggggaaaaga ccgtgcgtcg gtggcgtcca gccagcgtaa gcaaggtctt ttcccctgaa
     6901 gtcagccacg ccccagcggg gatgacttgc atttctccgc aaggagaaaa aagcaacaga
     6961 gataggttaa cgtaaaatgt tcagcgaaaa accgacatgg ttcacctatc ctgataattt
     7021 atcgtcaggg aatagaaaga cttaatcagg ggacaatatc ccacatcagc gcgtccggtg
     7081 accgtcttgc aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc tacgctccgg
     7141 gcgtgagact gcggcgggcg ctgcggacac atacaaagtt acccacagat tccgtggata
     7201 agcaggggac taacatgtga ggcaaaacgg cagggccgcg ccggtggcgt ttttccatag
     7261 gctccgccct cctgccagag ttcacataaa cagacgcttt ttcggtgcat ctgtgggagc
     7321 cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc cgacaggact taaagatccc
     7381 caccgtttcc ggtaggtcgc tccctcttgc gctctcctgt tccgaccctg ccgtttaccg
     7441 gatacctgtc cgccgttctc ccttgcggga agtgtggcgc tttctcatag ctcacacact
     7501 ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg
     7561 ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac ccgggaaagc
     7621 acgcaaaacg ccactggcag cagccattgg taactgggag ttcgcagagg atttgtttag
     7681 ctaaacacgc ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg aaggacagat
     7741 ttggttgctg tgctctgcga aagccagtta ccacggttaa gcagttcccc aactgactta
     7801 accttcgatc aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg
     7861 cagaaaaaaa ggatctcaag aagatccttt gatcttttct actgaaccgc gatccccgtc
     7921 agttcagagg aggacgatgg tgccaccgtt ccgccctgaa catcccggat ataatttgcc
     7981 tgacatgcgg ccagaagaaa gccagcaaca atcaatataa aaagaaaaat agtctttacc
     8041 tttttcatat actttccctg tatttaaaga ctccggcatt catccctgaa caccggaagt
     8101 cagtatttca aatcatcctt gataattttt tgttaccagt taccgtcccg ataatcaaaa
     8161 gctatataac aattatccac atcaggtttt atttgatttt taaaaaatgg ctgtagtaaa
     8221 ggaacccatt ctttcttcaa atcaaactca ccattattaa tattatcctt taaaggcatt
     8281 cccaagcttt caatgacaga accatcgtca ccaaagtctt tagaatactc ttccccttta
     8341 aactcttcag ttttcttatc aaaccatgtc aaacgtaatt taagccccat atttacctct
     8401 caaagatatt ttttaatgtt ccgttttgga tctggccctt taacctgctt acccgttttg
     8461 ggatcaaatg cgcctaggtg ttcgccatca ctggcacgat acccttcaag ctcaccgtgc
     8521 tgggaatccc attcataaat tttacgtttt ttatcgccat accatcgggc ccgcttacca
     8581 ccaccacctt gtttaggtgt tttaggatca cctcgcttta actctccaag tccctttatg
     8641 tcttctgttt taggtactgg atgataatca tggccgtagt ctttagtccc cttgcgaggc
     8701 ttgttctttt cctcatttaa cttattttct gcatccttcg cttttttctc cttttgtttc
     8761 cggctctcca tcgccccggt aagtgcagcc ttatctctgg ataaggattc attcattgcg
     8821 tttaaagatg cctgagccgc ttttaatttc tcattctgat tctgaatatc cttatcaaga
     8881 tagcgaacct gatcctgcca cccggagcca tattcatgag gcgggttata agtcacaaaa
     8941 tcatctttat ttgcctctgc ctcttttacc tttttatcgg cttcctgaac agcagccctt
     9001 gcaccgggga gcgccttctc tgtcgatgca atgcggctgt ttaaggtcgc aatattttta
     9061 tcttcggcat ccagctcagc tttcgcttta tcatagtctc ttttcagccc ctcttccgga
     9121 tgagcggcgt cccatgcctg ctggcgacgc ttttcttcct cctgacgctg ttttacctgt
     9181 gccggggtaa gaacatctgt caccgacaca taaaccggct tctgtccggt ctctttcggg
     9241 aaacgaataa cggcctcacg ggagttacca ccggctgtaa aaccagccgg acgtgaatca
     9301 cctttttcag caacaatgcc ttttggcggg gctttcgctg tcggaacacc tttcggtacg
     9361 ctcacctgca gagacgggag acccggaatc gacacactga atacccccgg acgttttgtc
     9421 ggtttcgcat ccaccacagg gacactcatt ggccgacctg cgacaaccgc aatatgctgc
     9481 cgctcatcct tcaccacatc cacaacccgt tgtctgacac gaaccgtcgc ctggtccagt
     9541 ggtaaagtac ttgccggagt ctccgtcacc gcatcggccg gtaatgacgt cataattttt
     9601 gacatcattt tcggatcatc ttttgcaatc tcagaaggca gcacaccgta gatcgcaatc
     9661 ccccacagac caaacttaaa cggccccttc agggcagcca gcacatcagc aacggcggcc
     9721 gacaacgcat cgccggaaac tgataaagcc agtccttcag cccccggagt agccagggca
     9781 ggcagaccga aggccatcgc agtggccgaa gactgtccgc caccagagct accgctatga
     9841 ttgccgctgt taccaccatt accaccgggt ttactcccgc cgttaccaaa ctcccccgga
     9901 ttatagttat gaatatctcc attcggcgta tgcgtcgtac cccagcccgg cccgctgttt
     9961 ggatccgtac caccacctga tgaagggcca cctttaccag acgtaccgtt cacattccca
    10021 ctggcctgac caccattatg tcccagacct gaattacccg gacctcgacc gtctccacca
    10081 ctcataccgt tttcctcttt gacatttaaa acaataaatt aaagccaaca tgtcgcggta
    10141 gggatacccg ttaccggata ccccccgcac agatcccggc gtgcgcgatt tacgcaccgg
    10201 gctcct
//
