LOCUS       NZ_CP068604             4382 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain 59493 plasmid p59493-4.382, complete
            sequence.
ACCESSION   NZ_CP068604
VERSION     NZ_CP068604.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17348255
            Assembly: GCF_016772535.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Chen,L. and Kreiswirth,B.
  TITLE     Genome sequencing of apramycin resistant K. pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Chen,L. and Kreiswirth,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 ideation way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:24:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,770
            CDSs (total)                      :: 5,650
            Genes (coding)                    :: 5,494
            CDSs (with protein)               :: 5,494
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 66 of 156
            Pseudo Genes (incomplete)         :: 90 of 156
            Pseudo Genes (internal stop)      :: 22 of 156
            Pseudo Genes (multiple problems)  :: 19 of 156
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068604.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="59493"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p59493-4.382"
                     /geo_loc_name="USA"
                     /collection_date="2017/2018"
     gene            complement(join(4294..4382,1..475))
                     /locus_tag="JMZ77_RS26500"
                     /old_locus_tag="JMZ77_26500"
     CDS             complement(join(4294..4382,1..475))
                     /locus_tag="JMZ77_RS26500"
                     /old_locus_tag="JMZ77_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001749520.1"
                     /translation="MHALSILPSVARANLATKFSSHLKVIELFNTMQDSQVVVLSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTGQTVYFIE
                     PEHIEQLLADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIEDKFTHMLDELSAA"
                     /name="hypothetical protein"
     gene            complement(733..1143)
                     /locus_tag="JMZ77_RS26505"
                     /old_locus_tag="JMZ77_26505"
     CDS             complement(733..1143)
                     /locus_tag="JMZ77_RS26505"
                     /old_locus_tag="JMZ77_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874179.1"
                     /translation="MAFISLSNLAAKTGRIIQSQCVTATQTKKSTGAVELGFRLSKSAM
                     DETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEEDNKKLLSDDSFTQLSEGVIVFRLDDE"
                     /name="hypothetical protein"
     gene            2237..2431
                     /locus_tag="JMZ77_RS26510"
                     /old_locus_tag="JMZ77_26510"
     CDS             2237..2431
                     /locus_tag="JMZ77_RS26510"
                     /old_locus_tag="JMZ77_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041631.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3254..3337
                     /locus_tag="JMZ77_RS29120"
     CDS             3254..3337
                     /locus_tag="JMZ77_RS29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071532987.1"
                     /translation="MHEPPVQSDRCAFSGNYRLESNPERHD"
                     /name="replication initiation protein"
     gene            3639..4265
                     /locus_tag="JMZ77_RS26515"
                     /old_locus_tag="JMZ77_26515"
     CDS             3639..4265
                     /locus_tag="JMZ77_RS26515"
                     /old_locus_tag="JMZ77_26515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223149424.1"
                     /translation="MSPTLNKQASKERCAVEFATRKKSKTQRKKSKALSYRGLCFFCTL
                     VTGKCQWTSKMSKQDYIQLRTLTDQFYADNKGLQEALDGSNDGKVRGYGIVVIDLNGLV
                     FGIPLRSHLNHKFGFVSERSEGVKKGLDYTKALLIKKEEYVSRAYKIPTPEFTHINDNK
                     EKIQEDFNKFVNRYIEANVKKDENILRNYRYSTLKNYHKELGLED"
                     /name="hypothetical protein"
     Typing          complement(1713..1968)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.86"
                     /coverage="89.0"
                     /evalue="1.25e-96"
     Typing          complement(2093..2201)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.0"
                     /coverage="100.0"
                     /evalue="2.2800000000000002e-29"
     Typing          2850..3363
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.023"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgtgatgtag aaggcttaag acggcttgag aaacactacc ttttttctcg ataagcttgc
       61 cgagattttt cccttctttt tcgagagaac gaaagaagag gctcctttcc tgattagcaa
      121 acacaagctc aggatcagcg agaagttgct cgatgtgttc aggctctatg aaatagacag
      181 tttgtccagt catccttcct gtagttacag atttaacaga tacatttaga tgacgaatag
      241 ggaaaaaata ttttttctct aacatctcga taatcgccgg taagcgagtg acctcaaagt
      301 cggctttgac actgttacca gaactggtga ggtgatggcc ttcgatcagt gatgacaaga
      361 caacaacttg ggaatcctgc atcgtgttga agagctcgat caccttcaaa tgagacgaga
      421 atttagttgc caggttagcg cgggcaactg aaggtagtat tgataaagcg tgcatatgta
      481 tagcctcata tgtttttaaa aacataaact taagagacca aagccaatag catcgcacct
      541 atcagaatag aaaatgtcgt gttcacatcc ctcaaaagaa acgatttttt ctttattaga
      601 atgcaagttt atgaacataa tgccgcccat aacactccac ccttccaaag gtagtgatgt
      661 gttgaccaga aggcggagtt agtcgattgt ttggcgacgc tcactaactc tgcctttcct
      721 ttttggtcct tcctattcat catccagcct gaagacgatt acaccttcac ttagctgagt
      781 gaatgaatca tcggaaagta gcttcttgtt gtcctcttca tcctcagaaa ggcgaggaaa
      841 acctggtcgc aaggtcaatc taacagcaca cgatgagtaa ccgccgtgct tgtttttttg
      901 cccagacaca ccaaagccac cttcactaat tttcttaatc atccactggt tttcttcttt
      961 gtcgtaaaga atgtcgacac gatccccaat ggtaatacca gtctcgtcca tagcgctctt
     1021 gctcaagcgg aatcccagct cgacagcgcc cgtgcttttt ttcgtttgcg tggccgtaac
     1081 acactgcgac tggattatcc ttccagtctt cgctgccaag ttcgatagag agataaaggc
     1141 cataatacaa tccgttttag acatcgatca gttcttatac ggaagtgtac ccagggagcg
     1201 aggtgacttt caagatcttt tgtattgatt tttctagagc cgcaaaaaac ggcgataaga
     1261 aaccttaaat acaaaaacaa ttgctcgtat tgaacctcgt caagatacac atccagatcg
     1321 atctttatgt aagtaaaggg tatttttcta tggtgattta aagtcgagca acgtcagcgc
     1381 atccaaaaaa atcaccaagt aggtgcaaga aagccgctta tagaaactga tttttatgcc
     1441 tgtataggtt gccgatgaga tgaatttaac ataagagttc ttgcgcgcac cgctaaaacg
     1501 cgctgaacgc gattctgccg caaacaccgc aaaaagagat gttttccccg gggtaaaccg
     1561 gaaaaatgcg tcaggatcga tttcagctca ctgcatagct atgcatgaaa gtgaatgacg
     1621 atcggtttgg gcgcatacag tacccatgag acacattttc ggacattttc agttcgtgaa
     1681 tccctgactc attgctacat atctgaacag acacccctgg cagcgccccc aggggcgtcc
     1741 tgtaaaaaaa cacaccgcgc cgctagcagc acccctaata taagatgatg ttttttatga
     1801 aaatagtcag taccacccct aaaaagcggt gtcggcgcgg tgttgtagct gcgccaacac
     1861 cgctttttta aatatcacaa agagagcaag aagaactaat ttgtcataac tctctattta
     1921 taaagaaaaa tcagcaaaaa cttgtttttg cgtggggtgt gggcatttca ggcatgagaa
     1981 cagactcgcg tttttccgga gtatcagcgg aattattgat taagaaacac tcgacgcaga
     2041 gcgaaatttt atgcaacagc ggctgcactg cacagggatt atttatcagt tgatatgctg
     2101 tgccatgata cgtcctaaag aaacaggaca ggacaggaca ggatgtgcaa ttgtaatacc
     2161 gtcgcccgca acgtcttaca attaccacct gctcagggtc cccccgacac ccggaactga
     2221 cggagaacaa cggaaggtga acaaacaaca acaaactgcg ctgaatatgg cgcgatttat
     2281 caaaagccag agcctgacac tgcttgaaaa actggatgct ctggatgccg acgaacaggc
     2341 ggccatgtgc gaacgactgc acgaactcgc agaagaactc cagaacagca tccagattcg
     2401 ctttgaggcc gaaagtgaaa caggaacata acgaagctga cggaggcggt cacggctttc
     2461 ccgtgggcgt tttcgtcagc agacaagccc gtcagggcgc gtcagggggt tttagcgggt
     2521 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaatat
     2581 gttagggggc tgatgctcag tcgaacgaaa actcacgtta ggcgagagaa gtcgtctgaa
     2641 aaatcgatta atggacaatg gtgtccatta agtgccattt gccgattaaa aacacaccgc
     2701 tttaggcaca tttttcactg tccacaatat tctgatttat cggacaaaaa gtccttaacg
     2761 tgtgaaaccg ttccgttgag cgtcaaaccc ctgttacggc ggagtaacac tttataaaag
     2821 tgcttcatgt ggcaggggaa aatgcagctg cagggcgtac gggagaacat ggaggttgcg
     2881 gtgacattac gcctcctcgc tcactcggtc gcttccgctc ctgccgttcg gctgcggcgg
     2941 gcgttacctg cacacggata agagcaaagg catacggcat gacaaacagg gcaagaaaag
     3001 gatgcggcgc tgccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     3061 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3121 ggtggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     3181 ttatggccgc gtttatctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     3241 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tttccggtaa
     3301 ctatcgactt gagtccaacc cggaaagaca cgactaatcg ccattggcaa cggccactgg
     3361 taaacatgat gtgcgagaga tgtattgaga gttcttgaag tggtggcctt actgaggcta
     3421 cactggaagg acgaatttgg tgacacgtct cgcacaagac tgttaccacg gttagataat
     3481 ctgccaggat taaataacct tcgaaaaacc acctgccagg gtggtttttt cgtttcagga
     3541 gaagattacg ctggcaaagt aagtccagtc aagaacagct acgaaattga cttcacatat
     3601 aatttttgat ataagtaaat ctagaagtgg cggtaggaat gagccccacg ttaaacaaac
     3661 aagcttccaa agaaagatgt gcggtagagt tcgccacacg taaaaaatcc aagactcaac
     3721 gaaagaaaag caaagccctc tcttacagag ggctttgctt tttttgtaca ttggttacag
     3781 gaaaatgcca atggacatca aaaatgagca aacaggatta catacagtta agaaccctta
     3841 cagaccagtt ttatgctgat aacaaaggcc tacaagaggc tctcgacggc agtaatgatg
     3901 gaaaagtaag aggctacgga atcgtagtta ttgatctcaa cggattggtg ttcgggatac
     3961 ccttacgaag ccatttaaac cataaatttg gattcgtttc ggagcgatca gaaggtgtta
     4021 agaaaggact ggattacacc aaagcgttgc taattaagaa agaagagtat gtatcgcgag
     4081 cctataagat accaactcct gaatttaccc atatcaatga taacaaagaa aaaatacaag
     4141 aagatttcaa caaattcgta aacagataca tagaagcgaa cgtcaagaaa gatgaaaaca
     4201 tactcagaaa ctatcgttac tctacgctga aaaactatca caaagaatta ggtttagaag
     4261 actaaaaagg cggtctaacg accgcccttg cctttacgcc gcgctcaatt catccagcat
     4321 atgggtgaac ttatcttcta tctccttcca catctcgtca gaaagaggtt tatctttgta
     4381 gg
//
