LOCUS       NZ_CP068179             4721 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Acinetobacter ursingii strain FDAARGOS_1096 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP068179
VERSION     NZ_CP068179.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357265
            Assembly: GCF_016726345.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter ursingii
  ORGANISM  Acinetobacter ursingii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 677.6x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 02:27:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,327
            CDSs (total)                      :: 3,229
            Genes (coding)                    :: 3,132
            CDSs (with protein)               :: 3,132
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 58 of 97
            Pseudo Genes (internal stop)      :: 37 of 97
            Pseudo Genes (multiple problems)  :: 36 of 97
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068179.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Acinetobacter ursingii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1096"
                     /culture_collection="DSM:30867"
                     /db_xref="taxon:108980"
                     /plasmid="unnamed4"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            join(3719..4721,1..914)
                     /locus_tag="I6I53_RS16550"
                     /old_locus_tag="I6I53_16555"
     CDS             join(3719..4721,1..914)
                     /locus_tag="I6I53_RS16550"
                     /old_locus_tag="I6I53_16555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045126957.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPFAKKYTSGCLSFYEHDLSDQDKQQIMQNFEQALFPGMDQSQYRVLWIEHQDKKNEE
                     TGKRRLELNFLIPNIEILTGQRLQPYFDRADRSRVDLFKKITNYEYQLHDADDPLYQQD
                     IKHAKNLPKAVNEIKETLNILATKAVENGLITDRQSMKTWLLDLGLEISRETKKSLSIK
                     NPNNDDPNARPIKLKGAIYEQDFRLTEESERLTIAASERYRREAEQRNQSNIQRYAEYS
                     AKRSTELEQQYRQHQNRYTAETERSNERDITADRPDYSSNQEATATGHDSPIARTTTAD
                     QTATSELAAVERLEPRNSPSSQIKENPYYIEYSLDFTGMYNAYQQYLSGVRQQEQIQRH
                     HSNGEQSQLFKITGREHDNNNVRGETVRGVRPDSERELRIGQWNETAWNSKGGTLNESR
                     STVIADYRAATAAAQRATEAARASIAAHSGTEQNHRRIRELQQQTGEALQPVERERKRA
                     DQYHQETTESEVIRGFIARLGEQLKTAITEPFRAISDWVKSKERDQRNIANDDLYRNRE
                     ADSTIDGTASKETRLSATVSRTIRGFDQENIFKALDELDRRRELQIVQERKNDRGYDSP
                     SPF"
                     /name="hypothetical protein"
     gene            986..1225
                     /locus_tag="I6I53_RS16555"
                     /old_locus_tag="I6I53_16560"
     CDS             986..1225
                     /locus_tag="I6I53_RS16555"
                     /old_locus_tag="I6I53_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918185.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_005000146.1"
                     /translation="MELAIKKWGNSAAVRLPSILLESLNLKLDSRVEVFTQDEAIVIKP
                     IKSKISLEQLLADITSDNLHCEVDTGHAVGKEIY"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            1225..1566
                     /gene="mazF"
                     /locus_tag="I6I53_RS16560"
                     /old_locus_tag="I6I53_16565"
     CDS             1225..1566
                     /gene="mazF"
                     /locus_tag="I6I53_RS16560"
                     /old_locus_tag="I6I53_16565"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005402007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="WP_005000144.1"
                     /translation="MPQNYVPEEGDIIWINFNPQSGHEQAGHRPALVLSPSSYNKATSL
                     LVCCPMTTQIKGYPFEVKIVGNPENVALSDQVKSLDWVARSATKKGQAKSEELEEVRQK
                     IAALLKIST"
                     /name="mazF"
     gene            1828..2778
                     /locus_tag="I6I53_RS16565"
                     /old_locus_tag="I6I53_16570"
     CDS             1828..2778
                     /locus_tag="I6I53_RS16565"
                     /old_locus_tag="I6I53_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004993712.1"
                     /translation="MNNAQLTDFFTNLAHKPYCADELLYGLKIRPKKTAINMQYIQGNQ
                     PCMLHYFFFDIDRSDAVMAWHEENLPVPYWTAQTQKNGHAHLCYKLEIPLCTSEFGSQK
                     SIAYASKVQAALANKLGADVGYSHLITKNPFHKDWRVTFWSEQAYTLDYLADFVELPKK
                     LSKKQEVLGLGRNCTLFENARKWAYKAVRDYFHHYSSLEWDKAVLAHLEALNREFEVPL
                     PYSEVKATAKSIAKYCWNKFSYAGFSEWQSKNAERANAKGACSLGGKARSQQFNNLRQQ
                     ALDLHIEGVNNTKIAEYLNVSRKTITRWLNQVVIQ"
                     /name="replication initiation protein"
     gene            2775..2996
                     /locus_tag="I6I53_RS16570"
                     /old_locus_tag="I6I53_16575"
     CDS             2775..2996
                     /locus_tag="I6I53_RS16570"
                     /old_locus_tag="I6I53_16575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004993714.1"
                     /translation="MSELDFFCYSLYVQKERKYKSNWAFVIFKVRYGKWISKSLRAQAI
                     AKNPTKEYLDWLYNYFEQNLDIVKAYNS"
                     /name="hypothetical protein"
     gene            3378..3722
                     /gene="mobC"
                     /locus_tag="I6I53_RS16575"
                     /old_locus_tag="I6I53_16580"
     CDS             3378..3722
                     /gene="mobC"
                     /locus_tag="I6I53_RS16575"
                     /old_locus_tag="I6I53_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_004993722.1"
                     /translation="MTAIKRNKVYKVRFSDAEVQKIKDANILNIAKYLRDTSLSQIDAS
                     QVNAKKKNLENHYTKLDREFLLELSRIGGNINQIAKAINTDLASSRPLDAARLLHLLIG
                     IDQTLKELKK"
                     /name="mobC"
     Typing          3720..4720
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.808"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gaacaacaac agcagatcaa acagctacaa gcgaacttgc agcagttgaa cggcttgagc
       61 caagaaacag cccaagcagc caaattaaag aaaaccctta ttacatcgaa tatagccttg
      121 atttcactgg catgtataac gcttatcagc agtatttgtc tggggtacgt cagcaagagc
      181 aaatacaacg acatcacagc aatggagaac aaagtcaatt atttaagatc acagggcggg
      241 agcatgataa caacaacgtg cggggggaaa ctgtgcgtgg agttagaccc gacagtgaaa
      301 gagaactacg cattggacaa tggaacgaga ccgcttggaa tagtaaagga ggtacgttaa
      361 atgaatctcg aagcacagtt attgcagatt atcgagcagc aacagcagca gctcaaagag
      421 caacagaagc agcaagagca agcattgcag ctcattcagg cacagaacag aaccatagac
      481 gaattagaga actacaacaa caaactggtg aagcactgca accagttgaa cgagaaagaa
      541 aacgagctga tcaatatcat caagaaacta cagaatccga agttatcaga ggatttattg
      601 caaggcttgg agaacagctt aaaacagcga ttacagagcc atttagagca attagtgact
      661 gggttaaatc taaagaacga gatcaacgaa atattgccaa cgatgatcta tacagaaata
      721 gagaagcaga cagcaccatt gacggaacag cttcaaaaga aactagactc agtgcaacag
      781 tatcacgaac gattcgaggt tttgatcaag aaaatatctt caaggcttta gatgagttgg
      841 acagacgtag agagttgcaa atagtgcaag aacgtaaaaa tgatcgaggt tatgattcac
      901 caagtccatt ttaatgctcg cgttattgac acaaaaatct tgctagggta ttgtatctac
      961 aatgtagata ctaggggttg ctgttatgga actagctatt aaaaagtggg gcaatagtgc
     1021 tgctgtccgc ctaccatcca ttttattaga aagccttaat cttaaattgg atagccgtgt
     1081 tgaggtattt actcaagatg aagcgatcgt tatcaagcca attaaatcaa aaatatcttt
     1141 agagcagctt ttagcagata taacatctga taatttgcat tgtgaagtgg atactggaca
     1201 tgctgtaggt aaggaaatct attaatgcct caaaattatg taccagaaga aggcgatatt
     1261 atctggatta attttaatcc acagagtggg catgaacagg caggacatag acctgcctta
     1321 gttttaagtc cttcatctta taataaagcc acaagcctac tagtttgctg tccaatgaca
     1381 acgcagataa aagggtatcc tttcgaagtt aaaattgtag gaaatcctga aaatgtagca
     1441 ctatctgatc aagtaaaaag tttagattgg gtcgctcgat cagcaacgaa aaagggacaa
     1501 gctaaatctg aagaacttga ggaagtgcga caaaaaattg cagccttact gaaaatatca
     1561 acttgatatc atctatgaaa tcaaagggta aggtaataca taaatctttt taagattatt
     1621 tactgataca atagcctata taaatcaata agttggaaag tttaatgaag tgtgttaaat
     1681 tttcttttag gcgaaaaaaa agcaagcttg ccggctcact tttttctcat tgtttaacaa
     1741 atacttaaag catcattaaa cgcaaattag ttacttgtta ttttactgat gttttgagca
     1801 tttgcaacct ttagaacgtt gcataatatg aataatgctc aattaacgga tttttttact
     1861 aatcttgctc ataaaccata ctgtgcagat gagttactgt atggcttaaa aataagaccg
     1921 aaaaaaacgg caatcaacat gcaatacata cagggcaatc agccttgtat gcttcattac
     1981 ttcttttttg acatagaccg ttcagatgca gtcatggcat ggcatgaaga aaatttacca
     2041 gtgccttatt ggacagcaca aacacagaaa aacggtcatg ctcatctctg ttataaatta
     2101 gaaattcctt tgtgtaccag tgagtttggc agtcaaaaat caatagccta tgcttcaaag
     2161 gtacaagcag cgcttgccaa caaacttggt gcagacgttg gctatagtca tctcattacg
     2221 aagaatccat ttcataaaga ttggcgtgtc actttttgga gtgaacaagc gtatacgctc
     2281 gattatttag ccgattttgt agagttacca aagaaattaa gcaaaaaaca ggaagtttta
     2341 gggcttggtc gtaactgcac cttatttgag aatgctcgta aatgggcata taaggctgtt
     2401 agagactact ttcaccatta tagtagtcta gagtgggaca aagctgtttt agcgcatcta
     2461 gaggcattaa atcgagaatt tgaagtacct ttgccatata gtgaagtaaa agctacagca
     2521 aagagtattg ctaaatattg ttggaataag ttttcgtatg ctggatttag tgagtggcag
     2581 tctaagaatg ccgaacgtgc taatgctaaa ggggcttgtt ctttgggagg aaaagcacgc
     2641 tcacaacaat tcaataattt aagacaacaa gccttagatt tgcatattga gggtgttaac
     2701 aatactaaaa ttgctgaata tttaaatgta agccgtaaga cgattactcg ttggcttaat
     2761 caggtagtaa ttcaatgagc gagttagatt tcttttgtta ttcactatat gttcaaaaag
     2821 aacgtaaata taaatcgaac tgggcatttg ttatttttaa ggttcgctat ggtaaatgga
     2881 tatctaaaag tcttagagca caagcaatag caaagaatcc aacaaaagaa tatttagact
     2941 ggctatataa ctattttgaa caaaatttag atattgtgaa ggcatataat tcataaaaag
     3001 gggtgggaca gtgccgccat atcagatatc agccccttgg gtgctttttt cccatttcat
     3061 ttttaaaaat tattatttaa gggaatgagc aaggctgtgg gcaaagtctc gacctgtgta
     3121 ataaacttgt gtgcaactca acgtataact agaagagtta tgcggagttg tgcataatgt
     3181 ttattcacag attagtcgag gcgttcgctt gcgattgtcc ataaccttgc catgccctgc
     3241 tcaataaaat atacgtctag gggtctgtca ccgtatgaga acgcaaggat acatggagac
     3301 ccctccgcta cgctacagag ttccatgacc ttgttcaggg gcaagccccc gaaaccccca
     3361 aaagcaaagg caaaagcatg acagcgatca aacgtaataa ggtttacaaa gtccgatttt
     3421 ctgatgcaga ggtacagaaa atcaaagatg ccaacatcct aaatattgct aaatatttgc
     3481 gggatacttc gctttctcaa attgatgcca gtcaagtcaa cgccaaaaaa aagaatttgg
     3541 aaaatcacta cacaaaatta gatcgtgaat ttttacttga gctatcccga atcgggggca
     3601 acatcaatca gattgcaaag gcaataaata ccgatcttgc aagcagtcgt ccccttgatg
     3661 ctgcaaggct tttgcacctg ttaataggga ttgatcagac cttaaaggaa ttgaaaaaat
     3721 gattgttcag ttcttcaggt acggcaatgg tttaagcaaa gggccactag attatttgct
     3781 tggtaaagac cgtgatagag actatgcaaa agttttgcag ggtgatgttt ccgaagtatc
     3841 agggttaatt gatacatcac cttttgccaa aaaatatacg tcaggttgtt tgtcttttta
     3901 cgagcatgat ttaagtgatc aagataaaca gcagattatg caaaattttg agcaagcctt
     3961 attccctggc atggatcaga gccagtaccg tgttttgtgg attgagcatc aagacaagaa
     4021 aaacgaggag acggggaagc gaagattaga gcttaatttt ctaattccca atattgaaat
     4081 tcttacaggg caacgtttac agccatattt tgaccgagct gatcgttcta gagttgatct
     4141 atttaagaag attacaaact atgaatatca actgcatgat gcagatgacc cgttgtatca
     4201 acaagacatt aaacacgcca aaaacctacc taaagctgtt aatgagatta aggaaactct
     4261 caatatttta gctacgaaag ctgtagagaa tggattaatt acagatcgtc aaagcatgaa
     4321 aacgtggcta ttggatctcg gtcttgaaat cagcagagaa acaaaaaaaa gcctatctat
     4381 taaaaatcca aataatgatg acccaaacgc cagaccaatt aagttaaaag gtgcaatcta
     4441 tgagcaggat tttagactta cagaagaaag cgaacgactt actattgcag catcagagcg
     4501 atatagacga gaagctgaac agcgaaatca gtcaaatatt caaagatatg cggaatacag
     4561 tgcgaagcga agcacagagc ttgagcagca gtatcgacag caccaaaacc gatatacagc
     4621 agaaacagag cgaagcaacg aacgagatat tacagcagat cgaccagact acagctcaaa
     4681 ccaagaagca acagcaacag ggcatgacag ccctattgca a
//
