LOCUS       NZ_CP068178             5483 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Acinetobacter ursingii strain FDAARGOS_1096 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP068178
VERSION     NZ_CP068178.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357265
            Assembly: GCF_016726345.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter ursingii
  ORGANISM  Acinetobacter ursingii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 677.6x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 02:27:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,327
            CDSs (total)                      :: 3,229
            Genes (coding)                    :: 3,132
            CDSs (with protein)               :: 3,132
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 48 of 97
            Pseudo Genes (incomplete)         :: 58 of 97
            Pseudo Genes (internal stop)      :: 37 of 97
            Pseudo Genes (multiple problems)  :: 36 of 97
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068178.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Acinetobacter ursingii"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1096"
                     /culture_collection="DSM:30867"
                     /db_xref="taxon:108980"
                     /plasmid="unnamed3"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            join(4309..5483,1..658)
                     /locus_tag="I6I53_RS16520"
                     /old_locus_tag="I6I53_16525"
     CDS             join(4309..5483,1..658)
                     /locus_tag="I6I53_RS16520"
                     /old_locus_tag="I6I53_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="WP_201686731.1"
                     /translation="MNKYIFEQLILNFFIFGPLVMGVSAGYQQQARERHAAGTQFRSGW
                     WIKVCSWGVMFYTACFALWCWIAPDFNFFWDYIKYGVPPIAVYVVACKLVSLSYQNWVP
                     VTVSKSPRSSAIHKYYDIFDTSNMWKVLTVTVDYGSPLVCLGNEHEIDPNNPDRIYTAE
                     EKAWCIADDKARVSNEDRGLVVGAPGSGKTTYLVAQLIDWMESGKSFVATDIKPEIWGI
                     LKENGVFDRFGYTDWVINPTDTASHHYNIFSEAMSSAELNEILAVVIPNTDESAEVFAE
                     NARRLLKAVIMELGKGASLPACQRYINRCDDVPELLKQLRQSDNEVVVSIAKDITRTAK
                     NDNLLSSIMTSLSRAFAFLDDDRIRASTADSDVALKEVLLKPRQAVFLQFDQQYKSTTA
                     TLFGAMVAHTLRILQSNYRKRDEVFVALDEIINCSPIPKFIDTLNTMRSAKMPTFLYFQ
                     ALEGLNRVYGAGADRLFMGACDFKAVYKISDIETAKVFSDLIGVTEGTITDKAYGTANN
                     TNAVNERSMLGVSIADAYSSGQSNQDNTMIRTSIMQVIEPDNFTKLQQYQAVCIYRGEA
                     TIFNMPVYHEHYPVPNRPQYPTVGEYEEHIGVVA"
                     /name="type IV secretion system DNA-binding
                     domain-containing protein"
     gene            complement(687..1628)
                     /gene="repM"
                     /locus_tag="I6I53_RS16525"
                     /old_locus_tag="I6I53_16530"
     CDS             complement(687..1628)
                     /gene="repM"
                     /locus_tag="I6I53_RS16525"
                     /old_locus_tag="I6I53_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641836.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_201640258.1"
                     /translation="MKNSLIVKDNALINASYNLELTEQRLIMLAIINARESGQGITADT
                     KLEIHASDYAKLFNVSIDASYKALREAVNNLFNRQFSYTAEYKRTGKTGVVRSRWVSRI
                     FYVDDLALLEITFSPDVVPLVTRLEEHFTSYQAKQVAHLTSKYATRLYELLIAWREVGK
                     VPTINIGEFRDRLGLLDDDYIAMSDFKKRVLEPSIKQINEHTDITVTYEQHKKGRLISG
                     FSFRLKRKQQTKQAESKRDPDTGDLFTKMTDAQRHLFGHKLAHDARVQSEYSQLIGTGS
                     YEAFAKLLADMLAEEKHFKTFYPLLIEHGFKK"
                     /name="repM"
     gene            complement(2519..2710)
                     /locus_tag="I6I53_RS16530"
                     /old_locus_tag="I6I53_16535"
     CDS             complement(2519..2710)
                     /locus_tag="I6I53_RS16530"
                     /old_locus_tag="I6I53_16535"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_201640259.1"
                     /translation="MKITAKKSKLERENFIKLKIFQKNTYKKTKTAYIDEVGIKYLISC
                     KIFEFKLKIFKKLARESA"
                     /name="hypothetical protein"
     gene            2786..3103
                     /locus_tag="I6I53_RS16535"
                     /old_locus_tag="I6I53_16540"
     CDS             2786..3103
                     /locus_tag="I6I53_RS16535"
                     /old_locus_tag="I6I53_16540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_201640260.1"
                     /translation="MKLFKFLNRQNTAITAPVINSACYTTDDLLILSTSVGQFTFDPVD
                     CDFAGVDDDVILFLFARKNANNRVENFNLNLDNSLKNKSKNKEIIAKFLEIKKRKSSIK
                     S"
                     /name="hypothetical protein"
     gene            3177..4070
                     /locus_tag="I6I53_RS16540"
                     /old_locus_tag="I6I53_16545"
     CDS             3177..4070
                     /locus_tag="I6I53_RS16540"
                     /old_locus_tag="I6I53_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017402718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="WP_201686729.1"
                     /translation="MTLEYQFNMLCNKHREGSHATQANRRAMLKMIAEQLKVCGYNTFR
                     MSPSDLKGRHVNKLLEQWRKDGVSTGTIKNRMTVIRWWADKVGNSGAVQSNDTYKIEKR
                     VYITNENKSISLNDLDLSKVDQSIAQSLRLQDAFGLRREESMKFQPEYALDGQRIENAK
                     YIRLKDSWTKGGRPRTIPITSDKQRQELRSAYTQAAINGGSLIPKEKSYKAHLASFERI
                     TSALGVGQTHGLRHGYAQTRYLELMGFDCPAVGGYRELTEAEKAKDKEIRMQISEELGH
                     SRINITSVYLGSWSKK"
                     /name="phage integrase N-terminal domain-containing
                     protein"
     gene            4070..4309
                     /locus_tag="I6I53_RS16545"
                     /old_locus_tag="I6I53_16550"
     CDS             4070..4309
                     /locus_tag="I6I53_RS16545"
                     /old_locus_tag="I6I53_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_201686730.1"
                     /translation="MGVASEIREKLPLCGRCINCKLLIWNDKAELKTSQTLIKFKNLDS
                     SYYYTVRCSWLKSPVSEPQFLDTCEGKQSQRGAE"
                     /name="hypothetical protein"
     Typing          complement(694..1628)
                     /name="rep_cluster_1219"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.6"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcaatataag tcaactacag caacgctttt cggtgcgatg gttgctcata ctctccgtat
       61 tttgcaaagc aactatcgca agcgtgatga ggtttttgtg gcgttggatg aaatcattaa
      121 ctgttcccca atccctaaat tcattgatac gcttaataca atgcgtagtg ctaagatgcc
      181 tacattcctt tattttcaag ccttagaggg gcttaatcgt gtctatggtg caggtgctga
      241 caggctgttt atgggggctt gtgattttaa ggcagtgtac aaaatcagtg atattgagac
      301 cgccaaggta tttagcgatt taattggtgt tactgaggga acgattacgg ataaggctta
      361 tggtactgct aataatacta acgcagtcaa cgagcgatct atgcttgggg tgagtattgc
      421 tgatgcctat agtagtgggc aatctaatca ggacaacaca atgattagga catcgattat
      481 gcaggtaatc gagcctgaca atttcacaaa attgcagcaa tatcaagcag tatgtatata
      541 tcgaggtgaa gcaacaatat ttaatatgcc tgtctatcat gagcattatc cagttcctaa
      601 ccgtccacaa tatccaacgg tgggagaata tgaggaacac attggagtag ttgcttaaat
      661 aaaaaaacgg ctatatagcc gtttttttat ttcttaaagc catgttctat gagtagtgga
      721 tagaacgtct taaagtgttt ttcttctgca agcatatcag cgagtaattt tgcaaaagct
      781 tcatagcttc cagtacctat caattggctg tattcgcttt gtactcgtgc atcatgtgct
      841 agtttatgtc caaataaatg acgctgtgca tcggtcattt tcgtgaatag atcgcctgtg
      901 tccggatcac gttttgattc tgcctgcttc gtctgttgct tccgtttaag tctaaatgaa
      961 aagcctgaaa ttaagcgtcc ttttttatgc tgttcatacg ttacggtaat gtctgtatgc
     1021 tcattgatct gtttgataga cggttctagt acacgttttt taaagtcact cattgctatg
     1081 tagtcatcat ctaaaagccc taaacgatct ctaaattcac caatgtttat tgttggcact
     1141 ttgccaactt cacgccacgc aatgagaagc tcatacagtc ttgttgcgta tttactggtt
     1201 aagtgtgcaa cttgttttgc ttggtagctt gtgaagtgtt cttctaatcg tgtcaccagt
     1261 ggaacaacat caggcgaaaa agtaatttca agtagtgcta aatcgtcaac gtaaaaaata
     1321 cgactaaccc aacgtgagcg cacaacgcct gttttacctg ttcttttgta ttcggctgta
     1381 tagctgaact gtcgattaaa taagttgttt acggcttctc ttaatgcctt gtatgacgca
     1441 tcaattgata cattaaacaa ttttgcataa tcactagcat gtatttctag tttggtatcc
     1501 gctgtgatgc cctgccctga ttcccttgca ttgatgattg caagcatgat caagcgctgt
     1561 tctgttaact ccagattgta actggcatta attaaagcat tgtctttcac gatcaaactg
     1621 tttttcatag agcagcacat attattattt aattaacgga caatgtatat taacgtcatt
     1681 taaaggtcaa tatatttttt gtcctttaat taagagctaa tgtccgttta tataagagct
     1741 aatgtccgtt tatataagag ctaatgtccg tttatgtaag agctaatgtc cgtttataag
     1801 ctctgtaatg cacgtatatc aaggctttca gcttgcctaa aagcatttaa aagctttaaa
     1861 aataattaaa agcataacgg caggggagtg caactgccct aggtactcgc taaagctcgt
     1921 actctattgc tgttcgcttc gctcacagcg aggggcagtt ttggttaatt ctgtttgtga
     1981 aatctaaaag caaaagcaac tatgaattcg cttcgctcat agagatttat gctcgctccg
     2041 ctcggtctag gttcaaatgc aagtgataga aaaaattgaa tctatgattc aattttttta
     2101 aaagcggttt ttcagagggg atgatcgaat attttttatg tcgctcgtag acactcgcta
     2161 aaactgttca tttgaattta ataatttttc caataaataa aattttcaaa cctagaaaaa
     2221 attaacaaag ggggcggagt tgcccccgac tagacacaat caaaatgcaa aaactttcct
     2281 ccctgattac aagcttcctt tggatttttt ttcgggagta gccgagccat cccgataggg
     2341 tggcgacttc cctttttgat actcgaaaac tgttgcctta cggctagcgt cactaccata
     2401 taggtagtga cgctagccgt aaggcttagt atatttacaa ttgtagacct tttttttagt
     2461 gatggatgcg tgacagaatt atttttgatg atataatgat cttacttttt tatgcttttc
     2521 atgcactttc ccttgctaat tttttaaata tttttagctt aaactcaaat attttacagg
     2581 agatcaaata cttaatacct acctcatcta tatatgcagt cttggttttt ttataagtat
     2641 ttttttggaa aatttttagc tttataaaat tttctcgttc aagttttgat ttttttgcag
     2701 ttattttcat tctgattgtg acttgtaaaa catttatttt tttaaatgat cttaacactg
     2761 acctttttta tggatctctt gtcttatgaa gctttttaaa tttttgaacc gccaaaatac
     2821 tgctataaca gctccagtta tcaattccgc ttgttataca actgatgatt tattgatttt
     2881 atcaacttct gttggtcagt ttactttcga ccctgtcgat tgtgattttg cgggtgttga
     2941 tgacgatgta attctatttt tatttgctcg aaaaaatgca aataatcgag tagaaaattt
     3001 caatttaaac ttagataatt ctttgaaaaa caaaagtaaa aataaagaga ttattgctaa
     3061 attcctcgaa ataaaaaaac ggaaatcctc aataaaatct tgatgatgcc tttttaactc
     3121 cgtagcaccc catacgggtg ctgtactcgt taaaaacgca tctcgttagg tgatctatga
     3181 cactcgaata tcaatttaat atgctgtgta ataagcacag agagggttct cacgccactc
     3241 aagccaatag gcgggcaatg ctcaagatga ttgcagagca actcaaggtt tgcggttaca
     3301 acacatttcg catgagtcca agcgacctaa aaggtcggca tgttaataaa ttactggagc
     3361 aatggcgtaa ggatggagta agcacgggca cgatcaaaaa ccgcatgaca gtgattaggt
     3421 ggtgggctga taaggtcggc aatagtggtg cggtgcagag caatgacacc tacaaaatcg
     3481 aaaaacgtgt ctatatcaca aatgaaaata aatccatatc tttgaatgac ttagatttgt
     3541 cgaaagttga tcaatctatc gcacagagcc tgcgcctaca agatgcgttt ggtttgcgtc
     3601 gagaagagag tatgaaattt cagccagaat atgcgcttga tggtcaacgt attgaaaatg
     3661 cgaaatatat tcgcttaaaa gatagttgga ctaagggtgg aagacctcgc acaatcccga
     3721 ttacaagcga taagcaacgg caagaattgc gtagtgccta cactcaagct gcgataaatg
     3781 gggggtcgtt aatccccaaa gagaagtcat acaaggcaca tttagccagt tttgaacgta
     3841 tcaccagtgc gttaggtgtg ggacaaacgc atggtttaag gcatgggtac gctcagactc
     3901 gatatttaga gctaatgggc tttgattgtc ctgcggtggg tgggtatagg gaactgacag
     3961 aagcggaaaa agcaaaagat aaggaaatac gcatgcagat cagtgaggag ttagggcata
     4021 gccgtatcaa cattacgtca gtttatttgg gcagttggag taaaaaataa tgggtgttgc
     4081 atcagaaatt agagaaaaac tgcctttatg tggtcgttgc attaactgta agttactaat
     4141 ctggaatgac aaagcagaat taaaaacaag tcaaacattg attaaattca aaaacttaga
     4201 ttcatcttat tactatacag tgcgttgctc atggcttaaa agtccagtat ctgaaccaca
     4261 gtttttagat acttgcgagg gtaagcaatc acaacgaggg gctgagtgat gaacaaatat
     4321 atatttgagc agcttattct taattttttt attttcgggc cacttgtaat gggagtgagt
     4381 gcgggatatc aacagcaagc acgagaacgg catgccgctg gtactcagtt tcggagtggg
     4441 tggtggatta aagtatgctc ttggggtgta atgttttaca cggcatgctt tgctttgtgg
     4501 tgttggatag ctcccgattt caattttttt tgggattata ttaaatatgg tgtccctcct
     4561 atcgctgtct atgtcgtggc gtgtaagctt gtctctctgt cataccaaaa ctgggtgcct
     4621 gttaccgtaa gcaaaagccc aagatcaagt gcgatccaca aatactatga cattttcgat
     4681 acgtcaaata tgtggaaagt ccttacagtc actgtggact atggctcacc ccttgtatgt
     4741 ctaggcaatg aacacgagat agacccaaat aatccagatc ggatttacac ggcagaggaa
     4801 aaagcatggt gcatagcaga tgataaagca cgagttagca acgaagatcg gggcttagtc
     4861 gtgggcgcac cagggtcggg taagacgact tatctagtgg cgcaactcat agattggatg
     4921 gagtctggta agtcatttgt tgctacggac atcaagccag aaatttgggg aattctaaag
     4981 gaaaatggtg tttttgaccg ttttgggtac acagattggg taatcaatcc gacagataca
     5041 gcaagtcatc actacaatat tttctctgaa gcaatgtctt ccgcagagtt gaacgagatc
     5101 cttgcggtgg tcatccccaa tactgatgag agtgcggaag tttttgcaga aaatgcccgc
     5161 cgactcctga aggctgtcat tatggagctt ggcaaggggg cgagtttgcc tgcgtgtcaa
     5221 cgctatatca acagatgtga tgatgtacca gagcttttga aacagcttcg tcagtctgat
     5281 aatgaagtcg ttgtttcaat tgctaaagac atcactagaa cagcaaaaaa cgataattta
     5341 ttatcatcta ttatgacaag tctaagtaga gcctttgctt tccttgatga tgaccgcatt
     5401 agagcgtcta cggctgatag tgatgtggct ttaaaagagg tgttattaaa gccaagacag
     5461 gcagtttttt tgcagtttga tca
//
