LOCUS       NZ_CP068142             3407 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1076 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP068142
VERSION     NZ_CP068142.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357245
            Assembly: GCF_016727425.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Blissenbach,B., Krut,O., Tallon,L., Sadzewicz,L., Zhao,X.,
            Boylan,J., Ott,S., Bowen,H., Vavikolanu,K., Mehta,A.,
            Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Blissenbach,B., Krut,O., Tallon,L., Sadzewicz,L., Zhao,X.,
            Boylan,J., Ott,S., Bowen,H., Vavikolanu,K., Mehta,A.,
            Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 7.1.0, HGAP v. 4
            Genome Coverage                   :: 803.04x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 02:34:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,280
            CDSs (total)                      :: 5,146
            Genes (coding)                    :: 5,013
            CDSs (with protein)               :: 5,013
            Genes (RNA)                       :: 134
            rRNAs                             :: 10, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 80 of 133
            Pseudo Genes (incomplete)         :: 57 of 133
            Pseudo Genes (internal stop)      :: 29 of 133
            Pseudo Genes (multiple problems)  :: 27 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068142.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1076"
                     /culture_collection="PEI:PEI-B-P-08"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="Germany: Langen"
                     /collected_by="Paul Ehrlich Institut"
     gene            430..621
                     /locus_tag="I6I33_RS26215"
                     /old_locus_tag="I6I33_26210"
     CDS             430..621
                     /locus_tag="I6I33_RS26215"
                     /old_locus_tag="I6I33_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            621..1022
                     /locus_tag="I6I33_RS26220"
                     /old_locus_tag="I6I33_26215"
     CDS             621..1022
                     /locus_tag="I6I33_RS26220"
                     /old_locus_tag="I6I33_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_077724207.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGSFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          3001..3215
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.105"
                     /coverage="100.0"
                     /evalue="7.539999999999999e-48"
     Typing          complement(2504..3020)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.847"
                     /coverage="87.0"
                     /evalue="5.5e-153"
ORIGIN
        1 gtttattttc atgttgttac agatgattgt aatacgttgc gtattacgct aatacacgaa
       61 ataactccat ttttttcatt taattcatgc agttgcgata aaaatcgcgg ctgcgttagg
      121 tgtatgacca tttaaggggc aaaaaattat cattctggct gggtgatctg cgcaggttaa
      181 ggatcatcgg cgacgaaaaa gcgtctttaa ctgcttcgtt catgcttgca tgaacggatt
      241 atttttccag actacaggta aactatagga agactacaaa agttgatgat ttaatcctgc
      301 gttttagtgc gatgactaca gataaactac aaaataacta caggataacc tcagatccgt
      361 tctctgaacc attcgtacat ccgaccccct taccggttta cactgagcaa tgatgctctg
      421 agggccctta tgacgaaaca acagaaaaca gcgctgaaca tggctaaatt catccagaat
      481 cagtctctgc tcctgctgga gaagcttaac gagcttgatc tcgatgttga ggcggacctt
      541 tgtgagaaac tccacgatga tgcggagcat cttttccgta cactgtcctc gagactggat
      601 gcattacagg atggtaactg atggccagag tgtctattag tgaggctgcc cgactgacgg
      661 gtaagagtag aacaacactg cacaggttaa ttaaagcagg tgacctgtcc acctgtagcg
      721 gtgaacgcaa tgcaaaaatg gtagatactt cagagctttt acgtgtcttt ggttcctttg
      781 aacaaccgaa gggtgaacac cattctggac aggtctctgg acagcgtgat acagcctctg
      841 ctgaccagag tgaacaggtg attcgtcatc tgaaacagga gattgaacac ctgaacacct
      901 tacttttgtc caaggattcg cacattgaca gcctgaaaca ggccatgctc cttcttgaaa
      961 atcaccaggc aaaattaact gaatccccgg cgccatggtg gaaattctgg aaaaaatcct
     1021 gacaccgtca tgcttcagtt ttcctctccg gccattccgg ggagttcctg aaccttccca
     1081 aaaatcgtca gatctctgaa cgcacctcac cacttaaaat acccacacac cacgcaaaat
     1141 taaaattttg cagatatctc attaatatca gtttgttagg tgttattaaa ttacgaatta
     1201 ttaccttctg taaggtaaat ccaagtcgtc ggcgattcgt gtacgacttg ccgacgactc
     1261 gtcatagagt catcgacgac tcacataaga ctatcagtaa cttatgacga cttgactcta
     1321 gagtcgaatc gtcattttgg tgtttttttg accttgacga ctctagagtc aagtcacgag
     1381 tcgaatcgcc ggtgaatcgt gtggtgagtc gtccggcgac ttgcataacg tgattgccgg
     1441 ccgtcgacga acccgattcc tgaacgtgct gaaaaccgtt tttccggctg cgccggcaac
     1501 gcttcacggc cacgcaataa attgcgtccc cgttcaccgt tttcagtctg gtttggtgcg
     1561 ggttttgagt tgtctggcct gaattcaggc tgaaatccgc caccagtcag tctggtgcat
     1621 aacaattcat gcccgtaaaa cgccctagag cgattttgag gcgttttcag ggggtaagac
     1681 ttaggattta atggattacg cccaagaatc gtttctagag cgtttgagcg gtgcgcgtaa
     1741 tgagacgtta tggtaaatct ctaaaggcaa tatttcccgt cccttggagc gaactactct
     1801 gtatttgatc tttggtgttg ataccccgct gcttgcagag ggggtattca ttatcgttgt
     1861 tgtttgctgt aattgtctaa aatatatttg gggatagata cgggtacaca ttcagcagat
     1921 attatgtttt tcccggatat attatgataa atgtctttgt ttgcaatgat tttatctttg
     1981 aattcaatgc attcttcatt cagaatgaag gtgttaacaa cattgtcttt cattcccaaa
     2041 tttgctaatt ctgaagaaca acttgcaata ttattcgtgt taaaacaagt tgttagtatt
     2101 attgcgtata tgtatggcat taaataaacc tatttaaaaa agtacggaat gatagtgatc
     2161 ttgttcggaa atatactagt cagactttaa gtctcaggga tatgaaccta gaacatcgtt
     2221 gtattaactt gtttgagtgt tccaatcctg aatattgatg ttatttgagt ataaaggaaa
     2281 cataataaat atcacaagga tattggttaa ctatttattt ttaaataatt tcttgagatc
     2341 tgacgttgga cgtcgtagtc tcttgccatg taaacgaaaa aaccaccttg cagggtggtt
     2401 tttcgaaggt tcagtaaacc ggcaagtttc tgaaccgtgg taacagtctt gtgcgagacg
     2461 tcaccaaatc tgtcctttta gtgtagcctc agtctggcca ccacttcaag aactcttcgt
     2521 aaatctctcg cacatcctgt ttaccagtgg ctgctgccag tggcgttaag tcgtgtcttt
     2581 ccgggttgga ctcaagacga tagttaccgg aaaaggcgca gcggtcgggc tgaacggggg
     2641 gttcttgcat acagcccagc ttggagcgaa ctgtctaaac ggaacggggc gtggtgattt
     2701 aggtaaagct tccaccacta cacggatgcc gcaggacggg aacaggagag cgcaagaggg
     2761 agccaccagg gggaaactcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     2821 ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg
     2881 ccgcggcctt atcgtatccc tgttaagttt cttcctggca tccaccagga aataaatttc
     2941 cgccccatta gtaagcctgc accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3001 tgagcgagga agcggaatat cacctgaacc agcatcttct cctgacgccg gaaagcctca
     3061 tttcactcct gccacatgaa gcactttggc aaatcaccac ttcggtttaa aatatttagc
     3121 ctgtatgcac tccgctagcg ctacgtgact ggttcagggc tgcgccccga aacccgctaa
     3181 cacccctcag ctactggata tcacaccatg ccatctgagt atgatgaaca accggatgcg
     3241 actgatgctc tgtttcccgg cgaatatgac ctttttgact taatggctca tgttactgaa
     3301 gagaacctgc acgccccggt ggattctggc ccgcctgtcg gccgggaagc gttatagctt
     3361 tttcgctggc cagcgtacat gtaatacgct ggtattacat gttattt
//
