LOCUS       NZ_CP068141             3991 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1076 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP068141
VERSION     NZ_CP068141.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357245
            Assembly: GCF_016727425.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Blissenbach,B., Krut,O., Tallon,L., Sadzewicz,L., Zhao,X.,
            Boylan,J., Ott,S., Bowen,H., Vavikolanu,K., Mehta,A.,
            Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Blissenbach,B., Krut,O., Tallon,L., Sadzewicz,L., Zhao,X.,
            Boylan,J., Ott,S., Bowen,H., Vavikolanu,K., Mehta,A.,
            Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 7.1.0, HGAP v. 4
            Genome Coverage                   :: 803.04x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 02:34:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,280
            CDSs (total)                      :: 5,146
            Genes (coding)                    :: 5,013
            CDSs (with protein)               :: 5,013
            Genes (RNA)                       :: 134
            rRNAs                             :: 10, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 10, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 80 of 133
            Pseudo Genes (incomplete)         :: 57 of 133
            Pseudo Genes (internal stop)      :: 29 of 133
            Pseudo Genes (multiple problems)  :: 27 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068141.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1076"
                     /culture_collection="PEI:PEI-B-P-08"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="Germany: Langen"
                     /collected_by="Paul Ehrlich Institut"
     gene            complement(111..223)
                     /locus_tag="I6I33_RS26480"
                     /pseudo=""
     CDS             complement(111..223)
                     /locus_tag="I6I33_RS26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            631..822
                     /locus_tag="I6I33_RS26195"
                     /old_locus_tag="I6I33_26190"
     CDS             631..822
                     /locus_tag="I6I33_RS26195"
                     /old_locus_tag="I6I33_26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040198051.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            822..1244
                     /locus_tag="I6I33_RS26200"
                     /old_locus_tag="I6I33_26195"
     CDS             822..1244
                     /locus_tag="I6I33_RS26200"
                     /old_locus_tag="I6I33_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040214596.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2048..2320
                     /locus_tag="I6I33_RS26205"
                     /old_locus_tag="I6I33_26200"
     CDS             2048..2320
                     /locus_tag="I6I33_RS26205"
                     /old_locus_tag="I6I33_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            2901..3875
                     /locus_tag="I6I33_RS26210"
                     /old_locus_tag="I6I33_26205"
     CDS             2901..3875
                     /locus_tag="I6I33_RS26210"
                     /old_locus_tag="I6I33_26205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_114269875.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     Typing          complement(112..583)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 accggcaagt ttctgaaccg aggtaactgg cttagaggag cgcagtcacc aaaacagttc
       61 gtttagttta gccttaactg gcgcataact tcaagactaa ctcctctaaa tcaattacca
      121 gtggctgctg ccaatggcga taagtcgtgt ctttccgggt tggactcaag agatagttac
      181 tggaacgggc gcagcagtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
      241 gaactgcctt cccggagccg agtatcaggc atggaatgag aaaacgcggc cataacagcg
      301 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccaccagggg
      361 gaaacgcctg gtatctttaa gtcctgtcgg gtttcgccac cactgatttg agcgtccgtt
      421 tccgcgatgc ttgtcagggg ggcggagcct atggaaaaac agccgttcgg cggcctttgc
      481 ttttctgcct catatcttgg ttgcctacgt ttttatgtac acgctcctgc cgctcgccgc
      541 agccgaacgg ctggagcgta gcgactgagt gagcgaggaa gcggaaaaga gactagtttg
      601 acattgagca ctgacgctct gaggcttctt atgacaaaac aacagaaaac agcgctgaac
      661 atggctaaat tcatccagaa tcagtctctg ctcctgctgg ataagcttaa cgagcttgat
      721 ctcgatgtcg aggccgacct ttgtgagaaa ctccatgatg atgcggagca tcttttccgt
      781 acactgtcct cgagactgga tgaattacag gatggtaact gatggccata gtgtctatta
      841 gtgaagcggc acggcttgtt aaggttagtc gccccacaat ttacaagatg attaacagtg
      901 ggaagttaag ttacacctct gtggtaaagc acgggaaagc tattaaagtt attgatactt
      961 cagagctaat tagagtgttt ggttctcttg atggtgtaat cgatactgta aaatatgatg
     1021 taaaaagtga cgcagaatct acaggtatta acagcgttgg tttacatgat ttacaacata
     1081 gaatcgcttt acttgaagct gaaaacgatg gtcttaaagg ggctgttaag gctcgagatg
     1141 agcatattga ttcccttcgt caggctatgc agcttctgga gcacaagcac gaaccttctt
     1201 caccacccca ttccccgtgg tggaaattct ggaaaaaatc ctgatcccgc catgcttcca
     1261 ttttgctctc cggccattcc ggggagttcc tgaaccttcc caaaaatcgt cagatccctg
     1321 aacgctcctc accactaaaa atacccacac actacgcaaa attaaaattt tgcagataga
     1381 cctttatttt caatcagtta tctattttaa attacgcaat tttatttttt gtaaggtgaa
     1441 tccgagtcgt ggacgactct atggcgaatc gccgatgact tgcccacgat tcatatgtga
     1501 atcacgccat ataatcagta cgttatgatg acttgactct agagtcgact tgccgggttg
     1561 gtggcttttt gaccatggcg actctagagt caagtcacga gtcgaatcgc cggtgaatcg
     1621 tgtggtgagt cgtccggcga cttgccataa tgtgattgct ggtctcgaca aacccgattc
     1681 ctgaacgtgc tgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg ccacgcaata
     1741 aattgcgcac ccgttcaccg ttttcagtct ggtttggtgc gggttttgag ttgcctggcc
     1801 tgaattcagg ctgaaatccc cagacagtca gtcaagtgca taacgattca tgcccgtaaa
     1861 acgccctgga gcgattttga ggcgttttca gggggtaaga cgtaggattt aatgggttac
     1921 gccccagaat cgttctgagg ccgttttagc ggtgcgtgta atgggacgtt atggtaaatc
     1981 aacggcggat gcctgagtct gtgttagagt cagcggattg tgataatcag tgactcaggg
     2041 agatgttttg gctactgacg ctcaggtaaa agaaattaat gcactcatca aaaaataccc
     2101 ggactcatgc tccatctgtc atgaagtcta tgatgaggat gatgtgacct acaccgtttt
     2161 tgggtatgac cggaaaggta aaatacaggt caccacggga tgctgtgccg gaatgctgac
     2221 agagccggtt ctgctgggtg tctgtgggtg ctttgacccg gaagaacgtg acgagatcat
     2281 gcagaatcac cccatggcaa agcagttttt cacagagtga ttccgtatct gttgagtctg
     2341 aagcccgcct tgtgcgggct ttttcatatc tgcgtgaaat gaggcgttat ggtaaatgca
     2401 cgcattcatt cttttgaaaa atccagaatg acgaatacat ggggcatgtt tatatgacta
     2461 ttgagaggaa ggatgttatt gtaaaaaaat cctttaaatt caatcattta attatggtag
     2521 ccaaaggggc ggggcttcgc ttattcctaa ggaataagcg aagccccgcc cctttggcta
     2581 ccagtgcatt atactttttt tttcacccaa tgtcaataca tgggttgatt ttatcatttt
     2641 aagcattttt gttgttgtga gtgctgggtt tatgccctat attcagcgct aagcaagcat
     2701 gggcttgcaa aggaacaatt aatgagaggt gctctatgaa aaaaatcaaa cttcacgatg
     2761 agcgtccaga cttgtaagtg tcaagtcagc agaaaaaggc agccaaggct gccttttttg
     2821 ttattggtta gtatatgatt gagcttatct taacttacct gaacaaagtt ttattgtttg
     2881 ctttgcgcaa ggattctctc atggcttttt ttaacctatt gtttgttgca tctctaattt
     2941 gttttggtgg tgttatgggg agctattcca gaggttgccg agactcccaa aataaatttt
     3001 caaaagataa ggatgagaat aataaaagag ctagtgttta tagatttggt atagcatccg
     3061 catttatttg tgttcctttc atatcatcat ttcttcatgt tgattactca agtataatat
     3121 ttcctgtggc tgatggtggt ggaactaagt tcatagaaca aatattgctt cttatttctg
     3181 tttcggggat ctctgcgtat cttgggtacg cattactgga tggtcttgcc aacaaagtat
     3241 taaaagagca agtagacggt atagataaaa agcaacaaga tttggaggct gaacaagatg
     3301 agtttaaaga tgagcttgat aggagcaagg agctgataga acagcttgaa atggataaga
     3361 aaacaacaaa attcgagcta ggatacttta aagccatttc agctgtggat aaagcagaat
     3421 caatgatgtc cattccggat gaggctcttt ctgttaagaa aaaactaact gaggcattag
     3481 atgctgtaac ggaatcattg tctcttgtta aaagagagga tgtagctaag gatgactacg
     3541 acaagctctt agtgcttaaa gcttatattt tgaaaagact tgatcgtatt gatgatgctc
     3601 tgtcaattac ggatgagtta cttatgagta atgaggataa ccctatactt atttacaata
     3661 aggcttgcta tcaatatatt ttgcgtcgtt gccaagctga taactccgat attaaggata
     3721 tgattcgcag ggctctgact attaaagtta cagatcctga attcattcgc aggcaagaaa
     3781 aaataagaac caaggtcata ggaaataaag ataatgatct tgaagggttg tttactgacg
     3841 ctgagctgga ggaattaaag gtggctatca aataggcagt ggctagctct atgaattcca
     3901 aaaagatcct tgacgatcct ttgaggtgca gcgtactcta cccctcaaac gaaaaaacca
     3961 cccggcaagg tggtttttcg aaggttcagt a
//
