LOCUS       NZ_CP068071             4397 bp    DNA     circular CON 06-FEB-2024
DEFINITION  Staphylococcus equorum strain FDAARGOS_1149 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP068071
VERSION     NZ_CP068071.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357318
            Assembly: GCF_016725305.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus equorum
  ORGANISM  Staphylococcus equorum
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 8.0.0, HGAP v. 4, SPAdes v. 3.11.1
            Genome Coverage                   :: 1545.66x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/06/2024 04:18:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,576
            Genes (coding)                    :: 2,546
            CDSs (with protein)               :: 2,546
            Genes (RNA)                       :: 86
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 16 of 30
            Pseudo Genes (incomplete)         :: 11 of 30
            Pseudo Genes (internal stop)      :: 10 of 30
            Pseudo Genes (multiple problems)  :: 6 of 30
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP068071.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Staphylococcus equorum"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1149"
                     /culture_collection="DSM:20674"
                     /type_material="type strain of Staphylococcus equorum"
                     /db_xref="taxon:246432"
                     /plasmid="unnamed2"
                     /geo_loc_name="Germany: Braunschweig"
                     /collected_by="DSMZ"
     gene            join(3872..4397,1..323)
                     /gene="str"
                     /locus_tag="I6J06_RS13215"
                     /old_locus_tag="I6J06_13210"
     CDS             join(3872..4397,1..323)
                     /gene="str"
                     /locus_tag="I6J06_RS13215"
                     /old_locus_tag="I6J06_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptomycin adenylyltransferase Str"
                     /protein_id="WP_031915899.1"
                     /translation="MRTEKEILNLVSEFAYQRSNVKIIALEGSRTNENIKKDKFQDYDF
                     AFFVSDIEYFTHEESWLSLFGELLFIQKPEDMELFPPDLDYGYSYIMYFKDGIKMDITL
                     INLKDLNRYFSDSDGLVKILVDKDNLVTQEIVPDDSNYWLKKPTEREFYDCCNEFWSVS
                     TYVAKGVFRREILFALDHFNNILRPELLRMISWYIGFNRGFDFSLGKNYKFINKYLTDK
                     EFNMFLATFEMNGYRKTYQSFKLCCELFKYYSNKVSCLGNYNYPNYEKNIENFIRNNYE
                     N"
                     /name="str"
     gene            593..991
                     /gene="mobC"
                     /locus_tag="I6J06_RS13220"
                     /old_locus_tag="I6J06_13215"
     CDS             593..991
                     /gene="mobC"
                     /locus_tag="I6J06_RS13220"
                     /old_locus_tag="I6J06_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_001002114.1"
                     /translation="MNEHKDNLASDLSVGGNRKPNRKEPKQISFRVSEQEYSKLKQSAE
                     TLNMTVPNFVKKKAQGSRLVSPKLDKETRQSIAKDLSHLGSNVNQIAKWFNQNKDKATH
                     LPEEKYHDLEKQFDDVKKELHKIWQQLN"
                     /name="mobC"
     gene            973..1935
                     /locus_tag="I6J06_RS13225"
                     /old_locus_tag="I6J06_13220"
     CDS             973..1935
                     /locus_tag="I6J06_RS13225"
                     /old_locus_tag="I6J06_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000246202.1"
                     /translation="MATTKLGNTKSASRAINYAEERAEEKSGLNCDVDYAKSYFKQTRA
                     LYGKENGVQAHTVIQSFKPGEVTAKECNEIGLELAKKIAPDYQVAVYTHTDKDHYHNHI
                     IINSVNLETGNKYQSNKEQRDFIKKANDQLCEERGLSVPEKSSEIRYTLAEQNMIDKDK
                     RSWKNDIRMAVEETKDNAVDFEEFNTLLKEKGVEITRVTKNNVTYRHIEEDKKVRGNKL
                     GDSYDKGVIENGFAIEKFRREREEEREYDEYADTFEVDWDAVAENSEDLRKRRIARTEE
                     TKQASNKIYIRDERTTGLERKGIAGNQVEFEKDDGGLSR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1904..2359
                     /locus_tag="I6J06_RS13230"
                     /old_locus_tag="I6J06_13225"
     CDS             1904..2359
                     /locus_tag="I6J06_RS13230"
                     /old_locus_tag="I6J06_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="WP_000753824.1"
                     /translation="MKKTTEDFQGRGKKVHNDFVRLLQKNLNRVNAEDIEIEVRKDVYG
                     VREEIHSMLREVKQSHEHYQKRQKQLFTGIGAMLLVFMLFALIMTIGRDFMGFLHVDVL
                     QNAIASKIKASEGFISVLWYIAYGLPYILAIGLFIWLYEWIRARFHD"
                     /name="DUF334 domain-containing protein"
     gene            2920..3864
                     /locus_tag="I6J06_RS13235"
                     /old_locus_tag="I6J06_13230"
     CDS             2920..3864
                     /locus_tag="I6J06_RS13235"
                     /old_locus_tag="I6J06_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167206.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_011167206.1"
                     /translation="MSKKAEEIQAKQSLEKENSNFSKTGYSNSRLNRHIMYTPEPKLHF
                     DAMTIVGNLNKNNAHKLSEFMSIAPQIRLWDILQTKFKAKALQEKVYIEYDKVKADAWD
                     RRNMRVEFNPNKLTHEEMLWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKSVK
                     KTIFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADIEVMSEHLWRVEIELKRDMV
                     DYWNDCFNDLHILKPDWSSLEKVKDQAMIYMLIHEESTWGKLERRTKNKYREMLKSISE
                     IDLTDLMKLTLKENEKQLQKQIEFWQREFRFWE"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          2921..3864
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.894"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          974..1932
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatttgcttt agatcatttc aataatattt tacgtcctga attattaaga atgatttctt
       61 ggtatattgg ctttaatagg ggttttgatt ttagtttagg aaagaattat aagtttataa
      121 acaaatattt aactgataaa gaattcaata tgtttttagc tacttttgag atgaatggat
      181 atagaaagac ataccaatct tttaagcttt gttgtgaatt atttaaatat tattcaaata
      241 aagtaagttg tttaggaaat tataactatc caaattacga aaaaaatatt gagaatttta
      301 ttcgtaataa ttatgagaat taattttatt agcaaccaca tttttggttg cttttgtttt
      361 gattttgaat ttggttttga actatgagtg gctagcattt tgccactcat tttttgcgtt
      421 agcaaaaaca taaagggtct gggattaatc ccagcaagcc ggtatattca gaacgaagtg
      481 gctagaatat acgacgcttg ccaaaccact atattacttg agaaaatata gattgtatta
      541 taagctatat ttatgaaaag gaatattttt cgtcagaaag gtggtgggac ttgtgaacga
      601 acataaagat aatttggcta gcgatttgtc tgttggggga aaccgtaaac ccaaccgcaa
      661 agagccaaaa caaattagtt tcagagtgag tgaacaggaa tattcaaagt tgaaacagtc
      721 ggcagaaact ttgaatatga cagtgcctaa tttcgttaag aaaaaggcac aaggcagtcg
      781 attagtgtcg ccaaaattag acaaagaaac gcgacaatcg attgccaaag atttaagtca
      841 tttgggtagt aatgtgaatc agattgctaa gtggtttaat caaaataaag ataaggctac
      901 acatttacca gaagagaaat atcatgattt agaaaagcaa tttgatgatg tgaaaaagga
      961 gttgcataag atatggcaac aactaaatta ggcaatacga agtcggcaag tcgtgccatt
     1021 aattacgcag aagaacgtgc agaagaaaaa agcggcttaa attgtgatgt tgattatgct
     1081 aaatcatatt ttaagcaaac tagggcgtta tatggcaaag agaatggtgt tcaagcacat
     1141 acagtcattc aatcttttaa gcctggcgaa gtaacagcta aagagtgtaa tgagataggt
     1201 ttagaattag ctaaaaaaat tgcaccagat tatcaagttg cagtttatac acatacagac
     1261 aaagaccatt atcacaatca cattattatt aattcagtaa atttagaaac aggcaacaag
     1321 tatcagtcaa acaaagaaca acgtgatttt ataaaaaagg cgaatgatca gttatgtgaa
     1381 gaacgtggtt tatctgtgcc agaaaagtca tcagaaatca gatatacgtt ggcagaacag
     1441 aacatgatag ataaagataa aaggtcatgg aaaaatgata taagaatggc agttgaagaa
     1501 acaaaggata acgcagtaga ctttgaagaa tttaatactt tgctaaaaga aaaaggtgtt
     1561 gaaatcacga gagtaacgaa aaacaacgta acgtatagac acattgaaga agataaaaaa
     1621 gtacgtggga ataagttggg cgattcttat gacaaaggag tgattgaaaa tggctttgca
     1681 atcgagaaat tcagacgaga acgagaagaa gaacgagaat acgatgaata tgcagacacg
     1741 ttcgaagttg actgggacgc agtcgcagaa aattcagaag accttagaaa acgaagaatt
     1801 gcaagaactg aagaaacaaa acaagctagt aataaaatat atatcagaga tgaaagaaca
     1861 acaggacttg agagaaaagg aattgcaggc aatcaagtcg agtttgaaaa agacgacgga
     1921 ggactttcaa ggtagaggta aaaaagtcca taatgatttt gttcgtttgt tacaaaagaa
     1981 tttaaatcga gtgaatgcag aagatattga gatagaggtt cgaaaagatg tttatggagt
     2041 tcgagaagaa attcattcga tgttgcgtga agttaaacaa tcacatgaac attatcaaaa
     2101 aagacaaaag caattattta ctggtattgg tgcaatgtta ttagtgttta tgctttttgc
     2161 tttgattatg acgattggca gagattttat gggcttttta catgtagatg tattacagaa
     2221 tgccatagca agcaaaataa aggcttctga gggctttatt tcagttttat ggtacattgc
     2281 ttatggttta ccttatatac tcgctatagg gctatttatt tggttgtatg agtggataag
     2341 agcaagattc catgattaaa caaagtatga tcaggctttt atgccgagaa aatttattta
     2401 tattgagaag tacccttaac taaacttgca gacgaatgtc ggcatagcgt gagctattag
     2461 cccgaccatt cgacaagttt tgggtttgtt aagggtacag aggctcaatg taaataaagc
     2521 aattggaata aagcaacaaa ggggttgaag aaaatgagta agaagaaaaa gattaattta
     2581 gcaatatcaa ttgctcaagt agtagtgcag gcgttaatac tttataacac ttatcagcaa
     2641 tataaatatt ctaaaaaata ttcagcctaa taaaaaaaga cttagtctga atagaccaag
     2701 ccttttgtta gtgttatatt aatatgcaaa taaaaaaaca aaaaaagaag tcgctcactc
     2761 cctgaccaaa gtttgtgaat gcgacttacc ttaaagaaaa acagttggtg ttttttttct
     2821 taatttttgt atatctagat attaaacgat attggtttat tcttcaagat atatattcgg
     2881 gtgagcgact tcttaaacaa aataaggagt cgatttttta tgagtaaaaa agcagaagaa
     2941 attcaggcaa aacaaagttt ggaaaaggaa aactcaaatt tttctaaaac cggatactct
     3001 aatagccggt taaaccgaca tattatgtac actcccgaac caaaattaca ctttgatgct
     3061 atgacaattg ttgggaatct taataagaac aatgctcaca aactgtctga atttatgagt
     3121 attgcgccac aaattagact ttgggatata ttacaaacca aatttaaagc taaggctcta
     3181 caagaaaaag tttacatcga atatgataaa gtaaaagcag atgcgtggga tagacgtaat
     3241 atgcgtgttg aatttaatcc taataaactt acgcatgaag aaatgctttg gttaaaacaa
     3301 aacatcattg actacatgga agatgatggt tttacaagat tagatttggc ttttgatttt
     3361 gaagacgatt tgagcgatta ttatgcgatg actgataaat cagttaagaa aactattttt
     3421 tatggtcgta acggtaaacc agaaacaaaa tattttggtg ttcgtgacag tgatagattt
     3481 attagaattt ataataaaaa acaagaacgt aaggataatg cagacattga agttatgtct
     3541 gaacacttat ggcgtgtaga aattgaatta aaaagagata tggttgatta ttggaacgat
     3601 tgttttaatg atttacatat tttgaaacca gattggtcta gtttagaaaa agtaaaagac
     3661 caagcaatga tttatatgct aattcatgaa gaaagtacat ggggtaaatt agaaagacgt
     3721 acaaaaaata aatacagaga aatgttaaaa agtatttcag aaattgattt aacggactta
     3781 atgaaattga ctttaaaaga gaatgaaaaa caattgcaaa agcagattga attttggcag
     3841 cgtgaattta gattttggga gtgaaaaaac aatgaggact gaaaaagaaa ttttaaattt
     3901 agtttctgaa tttgcttatc aacgaagcaa tgtaaaaatt attgctctcg agggttcaag
     3961 aactaatgaa aatataaaaa aagataagtt tcaagattat gattttgctt ttttcgtatc
     4021 agatattgag tatttcacac atgaagaaag ttggttaagt ttatttggag aattattgtt
     4081 tatacagaaa ccagaagata tggaattatt cccacctgat ttagattatg gttacagtta
     4141 tataatgtat tttaaagatg gcataaaaat ggatattaca ttaattaatt taaaagattt
     4201 aaatcgttat tttagtgatt ctgatggtct tgtaaaaatt ttagttgata aagataattt
     4261 agtaactcaa gaaattgttc cagatgactc aaattattgg ttaaaaaaac caacagaacg
     4321 agaattttat gattgctgta atgagttttg gagtgtctca acgtatgtag caaagggtgt
     4381 ttttagaaga gaaatat
//
