LOCUS       NZ_CP067429             7301 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli O22:H8 strain 154 plasmid p7, complete sequence.
ACCESSION   NZ_CP067429
VERSION     NZ_CP067429.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17217524
            Assembly: GCF_023518355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O22:H8
  ORGANISM  Escherichia coli O22:H8
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Marques da Silva,W., Bono,J., Amadio,A.F. and Cataldi,A.
  TITLE     Whole-genome sequence of Shiga Toxin-Producing Escherichia coli
            O22:H8 strain 154 isolated from cattle in Argentina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   Marques da Silva,W., Bono,J., Amadio,A.F. and Cataldi,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2021) EEA Rafaela, Instituto Nacional de
            Tecnologia Agropecuaria, Ruta 34 Km 227, Rafaela, Santa Fe 2300,
            Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023518355.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 05:46:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,714
            CDSs (total)                      :: 5,585
            Genes (coding)                    :: 5,388
            CDSs (with protein)               :: 5,388
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 79 of 197
            Pseudo Genes (incomplete)         :: 128 of 197
            Pseudo Genes (internal stop)      :: 46 of 197
            Pseudo Genes (multiple problems)  :: 51 of 197
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067429.1.
            Bacteria and source DNA available from Angel Cataldi - Instituto de
            Biotecnologia - INTA Castelar, Buenos Aires, Argentina.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Escherichia coli O22:H8"
                     /mol_type="genomic DNA"
                     /strain="154"
                     /serotype="O22:H8"
                     /isolation_source="Rectal swab of live bovine cattle"
                     /db_xref="taxon:2491679"
                     /plasmid="p7"
                     /geo_loc_name="Argentina: San Miguel del Monte, Buenos
                     aires province"
                     /lat_lon="35.45 S 58.75 W"
                     /collection_date="2016-09"
                     /collected_by="Luisina Martorelli"
                     /genotype="stx1-stx2d"
     gene            complement(join(5347..7301,1..289))
                     /locus_tag="JI426_RS27265"
                     /old_locus_tag="JI426_005379"
     CDS             complement(join(5347..7301,1..289))
                     /locus_tag="JI426_RS27265"
                     /old_locus_tag="JI426_005379"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="WP_050875988.1"
                     /translation="MTNSLGYSGTPISHYVDGVPYDFNGSPIIVITNGPEKPVINTGVL
                     DKLPPVDKPIFLDPSIFNKEVTSVSDLKKIIVALESAIKHLTSLLPSDINKFVSSVKTL
                     LGEVRGNSSFTVLVNELDTALSRLEKSIEDVNSGIVVVDERRHNVETMLNQFTDKFKYK
                     EFLDKSKQLGDFSYFEGAFIIGLASRPDTRWSITSWKEMVVLPNKELFDAQNELITKYN
                     HLLHEYEIVSKKVDILSVEKSRLESGTSLTAPEKPDLIEPSNNGKPDTLPGKAELLIPV
                     LPPPDIHIPEIPTIQALDLENIKTIELELADFKPADIDPVEIKPIELPSVDIKPWPSSA
                     LGSEVTDPVYTPEMVKKAESSMLAAGALVMGVAPQGMQLSVAGRGAWIPVAEIAGNIAT
                     RILSAVKSLMAVNPITLSASVTVAGLWPKEAGKNSDKVPGRDVEALFAVNARLLAEDIQ
                     MSEFSRMEHIPVRGAIVMDNGRPVIRFFNTGATGRFPFVPVLKAERDELTGLDKIIVPG
                     INGGPSRTILINPVPVPSVPTDTGNTSPVPVTPVHTGTEVKPVDNIGVTTSPVPEDIVL
                     NDFIYWQPGEHGTVYEPIYVMLSGIYGETNAKGKYSHRPYNTDKAGGPIQNLDWKNTEI
                     DRVGVDKVKLHTGRFEESDANKVMIDRLEKILKGELQVTDTDKRFYTHEIRELERYRNL
                     GVKDGTIPDNAGEVWNNTHTATLEDYKLSNDETLLYTPEALKAAEEQELRMLK"
                     /name="S-type pyocin domain-containing protein"
     gene            complement(<596..769)
                     /locus_tag="JI426_RS27270"
                     /pseudo=""
     CDS             complement(<596..769)
                     /locus_tag="JI426_RS27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530439.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(2660..2854)
                     /locus_tag="JI426_RS27275"
     CDS             complement(2660..2854)
                     /locus_tag="JI426_RS27275"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_001367294.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAKG
                     ENIGWCIARAEYCKKTGRF"
                     /name="ANR family transcriptional regulator"
     gene            3729..3923
                     /locus_tag="JI426_RS27280"
                     /old_locus_tag="JI426_005376"
     CDS             3729..3923
                     /locus_tag="JI426_RS27280"
                     /old_locus_tag="JI426_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041629.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELGNSIQTHFEAQYGTEP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4482..4643
                     /locus_tag="JI426_RS28655"
     CDS             4482..4643
                     /locus_tag="JI426_RS28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072033293.1"
                     /translation="MQDPPVQPDCCACSGNCSLEANPERHALTPLAAATGNWGHCGSLV
                     KRESLYKT"
                     /name="replication initiation protein"
     gene            complement(4842..4988)
                     /locus_tag="JI426_RS27285"
                     /old_locus_tag="JI426_005377"
     CDS             complement(4842..4988)
                     /locus_tag="JI426_RS27285"
                     /old_locus_tag="JI426_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_040077440.1"
                     /translation="MRKIKWIFGLVIIGVIVIAGCQANYIRDVQGGTVAPSSSSELTGI
                     ATQ"
                     /name="colicin release lysis protein"
     gene            complement(5078..5350)
                     /locus_tag="JI426_RS27290"
                     /old_locus_tag="JI426_005378"
     CDS             complement(5078..5350)
                     /locus_tag="JI426_RS27290"
                     /old_locus_tag="JI426_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040077442.1"
                     /translation="MIDFERMSKEENIADVIAFLVRKEGGFGYPQMDRFFSRHNFSVIE
                     SGEFMRVFEQLCQAGIVVLGDKMLVKKGPNWKEPQFVSDKKYGIS"
                     /name="hypothetical protein"
     Typing          1850..1928
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.75"
                     /coverage="87.0"
                     /evalue="3.37e-32"
ORIGIN
        1 acttattaat atcggaagga agcaatgacg tcagatgttt aattgcactt tctaacgcaa
       61 ctataatctt tttaaggtcg ctgaccgacg taacttcttt gttgaaaata gaaggatcga
      121 ggaaaatagg tttatccacc ggaggtagtt tatcaagaac acctgtatta attaccggtt
      181 tttctggacc gttggtgata acaataattg gactaccatt aaaatcataa ggaacaccat
      241 caacgtaatg tgatatgggt gtcccgctat atcctaaact attcgtcata ataaaatcct
      301 ttttaaaatg cgcacacaca acaactgtat atgcatacag tatcattgtg ctaattttgc
      361 tcgccttgtc aagaaggcta atttcttttt ttgtgataat catcacaaga aagggcttaa
      421 aacggataga tgagacctaa tgacagcaac tggccaaagc aagagaaggg gtcagtttgg
      481 agatcggaaa tttttgtacg ttaagcgcat tatttaacca atcccgatgc gtaacggatt
      541 gattttgcgt caacgtttat cgcttttgag gtattggagg tttgagaaag gggtgatccg
      601 ctacgctcac ctcagataca gcagccaggg cgctcagtgt ttccaaactt ggctgatcgc
      661 cgttctcgat acgctgcacc gtgcgcacgc ttagcccaca cagttcgtta agctgctcct
      721 gggactaggt acgcgcaaga cgcagtgacc taaattggta catggacatg tcagatttac
      781 cataatccct taattgtacg caccgctgaa acgcgctcag cgcgattctg gcgcaaaaac
      841 cgcaaaaata gatgttttcc cccgggtaaa ccggaaaaat gcgtcaggat tgttttcatg
      901 gcactgcatg actatgcatg aaactgaatg gcgatcggtt tgggcgcgcc tgatgcccat
      961 aaggcgcatt ttcggacgtt ttcagccctg atgagaagaa atcagacagt agttacagac
     1021 gagtcgtgag cgattcaccc cgggagtcat cagcgagtca tccagtattt tcctcgcgac
     1081 tctctggcga ctcgccttct ctgaacacca gagagacagt gtgttgagtc atcgataaat
     1141 caccgacgac tcgttgccga atcatccagt agtcgccgac gactcgcttt tgtatacacc
     1201 tgaataagaa aatatatttt tagaaccata acgacatgat ttaaaaagca aatcagcaaa
     1261 aagttggttt tgcgtagtgt gtgggcattt caggcatgag aacagactcg cgtttttccg
     1321 gaagaactgc ggggattttt gattaaacaa tactcaccgc agaacggaat tttatgcaac
     1381 gccggcagtg ctgcacgggg atttttaatc cccagcatgt cagagggtgc ggatgacggt
     1441 tcccgtctgg cgttttcatg ggaaccggtt gtcaaaaaat accagcgtga tcagtcgagg
     1501 gataagcgca gattaatcag tgatgattga gcgcgaatct gacaacgagt atagcgacac
     1561 agcaacaaaa aagacgccag aaacgcgctc agaggctttg taacgcgatt ttttacatga
     1621 ggcaatggct tcggttgttt tttcgctttg aggcgttcag gcgtgttcag ggcgcgatgc
     1681 tggtacatac ctgactgtaa tcatccctac gaactactct ttgcaccaga aacgcgcagg
     1741 agttttgtag cgcgtctccg gtccctgatc aatgaattcc cggtcattaa catgcgttta
     1801 cccggaaacg cgccctgatg ttctatcgta tcgcgggaac ggtgtcgagg gtgtcggggc
     1861 aaagccctga ccaggtggta atcgtatagc cgtgcgtgcg cggttatacg attacacatc
     1921 ctgtcccgga atcaggattg tttttcgcgc acaactgcgg caaattcatc ccgttgcggt
     1981 tgcaacgatg ggtatatcaa aactacgaaa attattcagg catttgctac cccccttgcc
     2041 ctggcctcca ccagtcacgg gggatttttt tgttgtctct catgacgaac tacggcaaat
     2101 ttctgacgtt gcaaactgca acacataaaa aacacacaaa actcacaatt gccatgtcct
     2161 cattgacacc cccttgccat atagggggat tttttcgcct gtcctgattg tcttttgccg
     2221 atggttacgg cagattggaa aagagcgcat ttgtggtgct ctctccagaa taatatttct
     2281 cattactcaa actctgatgg cttccccctg taatccaggg ggatttttta atttatcgct
     2341 atgcttcgta attcattcag aatacagaca gtatggttaa tcagtttttc atcatttttg
     2401 cggtgtttgc cctgtcctgc atcagaacgc taaaaaacag gattggaatc tctttatgac
     2461 gaatcctcca tttcaggatt ttttatgtaa cgtctactac atgcgatccg ttggtgaacc
     2521 gttagttata tattggttat atgttaatta tatcttagtt atacttttga tgcgcgtcag
     2581 ttacagaggc gttttgaggg taaattctgg aggcacgtcg acgaatcaaa gggggcgccc
     2641 ctttgattcc ccgccagaat taaaaccggc ctgtcttttt acaatattca gctctggcta
     2701 tacaccaacc tatattttct cctttggcat aatgaagcgc atcattccat tttttccatg
     2761 ctccaatgta atcaccttca cgctccagtc tgacagcttc gtttgcgggt gaaaaatatt
     2821 tattttctaa ataaattgct ttgtcctgat tcatactcgt atttatattt ttactggaat
     2881 taaaaagagc ggcctgtcgg gttaatacat attggcatga ctgttgatat atcagcccaa
     2941 ctgcacgctc gtaatcgttt taacgtgatt ttcgcaatat ggcaatccgg gttcgtgtgt
     3001 ccggtaatca ataaaaaacc gtgcgaaaaa cgggacagga tgtgcatttg tcggaccgcg
     3061 cacacacgac cggacaaacg ccacccggtc atggcgcgcc ccgacacccc gcgaacgtct
     3121 ctggccagaa ctggccatca cgccgggtca ctttgtcaga tgttaaaaaa tcacgcggta
     3181 aacgccgtta ttcgcgggtt tgcgcaagca cctcccgtgc gtcactttgt caaaacgtca
     3241 aagtgacgca catcaggaac cgccggaacg ttcatgttga tgacgacaac atgcacacaa
     3301 cgcagacaga aaatcgcgca catattcagc aaaaaataag cgtcacgggg tgttaatggg
     3361 ctgcgtttgg gaaacgaaca atattgtacg gttgcgctat cgcggttggg tgtactcccg
     3421 tgctatacgg cgttttcagg ggctttggcc ttaatcggat aacctaaatg cgacatagca
     3481 agagcgataa cttaaaggcg atagccgctg atgttaaatc aatacgttag aatcttaaat
     3541 tgattaaata atgcgcttaa cgtacaaaaa tttccgtttc ccaaaccgcc gtttcccaaa
     3601 ccgccccctt aatgtcgcgc agcccactca cgacggggct tgtgtgataa ttttcagtga
     3661 cgtcacctgg cgtgatgacc aggtgacgca cagccggacg taaacaccag taacggagaa
     3721 gggagaacgt gaacaaacag cagcagaccg cgctcaacat ggcgcgattt atcaaaagcc
     3781 agagcctgac gttgctcgaa aaactggatg cactggatgc agacgaacag gccgccatgt
     3841 gcgaacgact gcacgaactg gcggaagaac tcggtaacag catacagaca cactttgaag
     3901 cgcagtacgg cacggaacca taaaggggct ggcgaaagcg gtcgcggctt gtccgtgagt
     3961 gttttcgcca gcagacaagc ccggagggcg cgtcaggtca cgggctgaaa ggaacacagg
     4021 cgcgtaagtg ctcaaagcag acaggcggag aaattccgct tcctcgccca ctcgctcact
     4081 acgttcggtc gctggggctg cggcgagcgg tgacggctct ctcataacag cagacggatt
     4141 atccacagaa tcgggggata accaggacag aacatgtgag caaaaaagca cgaccagaaa
     4201 aggccgcggc aaagccgttt ttccataggc tccgcctccc cctgacgagc ttcacacttt
     4261 ccgaagctgc aagtctagga tttggcgaaa cccgatgtgg cttcaagatc atcaccgttt
     4321 ccggtgaatc gctcccccct gcgaccccca cattcccgcc atatcgtttg ccggatacct
     4381 taccgccttt ccccttgcgg gaccgtggcg ctttctcata gctcacgtgc atctctgcgg
     4441 tatctcgggc gacttaggca gttcgctcca ggtctggctg tctgcaggac ccccccgttc
     4501 agcctgactg ctgcgcctgt tccggtaact gttctcttga ggccaacccg gaaagacacg
     4561 ccttaacgcc actggcagca gccactggta actggggaca ttgcgggagt ttagttaaac
     4621 gtgaatcgct atataaaact tgaagacacc tgccgttaca ggctaaactg actgtacaga
     4681 tttggtgact gtctcccgca atagagtcca gttaccgccc ggtttcgaag agttgctagc
     4741 tcaacgaacc cgaactaacc cgccatccgg cgggtttttt cgtttacagg cagcagatta
     4801 cgcgcagaaa aaaaggatct caagagatcc tttgatcttt tctactgagt agcgatcccc
     4861 gtcagttcag aagaggatga tggcgcaaca gtccctcctt gtacatcacg aatatagttt
     4921 gcctgacagc cggcaattac aatcacacca ataataacca atccaaatat ccattttatt
     4981 tttctcataa tcaatcctta tcatgagtta aatatcagtc tcccatccat gaaagactga
     5041 tatttagaac aacagctaat ccattaactg tttctactta actaatgcca tattttttat
     5101 cagaaacaaa ttgaggttcc ttccaattag gtcctttctt aacaagcatt ttatcaccca
     5161 atacgacaat acctgcctga cataattgct caaaaactct cataaattcg ccactttcaa
     5221 ttactgagaa gttatgacga ctaaaaaaac gatccatctg agggtatcca aatccgcctt
     5281 cctttctcac tagaaatgca ataacatcag caatattctc ttcttttgac atccgttcaa
     5341 aatcaatcat tttagcatcc tcaattcttg ttcttccgcg gcttttaaag cttctggtgt
     5401 atataaaaga gtctcgtcgt tacttaattt ataatcttcc aacgtagcgg tatgggtgtt
     5461 attccaaact tcccccgcat tgtcaggaat ggtgccatct ttgacaccaa gattgcgata
     5521 cctttccagc tctctaatct catgggtata aaagcgttta tccgtatcag taacttgcaa
     5581 ttcgcctttc aggattttct ccaagcggtc gatcattact ttgttagcat ccgactcttc
     5641 aaaacgcccg gtatgtagtt tcaccttatc aacacctacg cgatcaatct cggtattttt
     5701 ccagtccaga ttttgaatag gaccgcctgc tttatccgta ttatatggac gatgactgta
     5761 cttcccttta gcatttgttt ctccataaat accactcagc atcacataaa tcggctcata
     5821 aacggttccg tgctccccgg gctgccagta gataaaatca ttcagcacaa tatcttcagg
     5881 aacaggtgag gttgtcaccc ctatattatc cacaggttta acttctgttc ctgtatgcac
     5941 tggtgttacc gggacagggg aggtattccc tgtatcggtc gggacagaag gtaccggtac
     6001 cggattaatc agaatggtcc gcgacgggcc accgttaatt cccggaacaa taattttatc
     6061 cagcccagtt agttcatccc gctccgcttt cagaactggg acaaacggga aacgccctgt
     6121 agctcccgta ttaaagaacc tgatgacagg ccgcccatta tccatcacta tagccccacg
     6181 gacaggaatg tgctccattc gggaaaactc agacatttgt atatcctctg ccagaagacg
     6241 ggcattaaca gcaaacaatg cctctacatc ccgaccggga accttgtcac tgttttttcc
     6301 tgcctccttc ggccataacc cagcaacagt aacggaagca ctcagagtta tcggattcac
     6361 tgccatcagt gatttcacag cactaagtat ccgcgtagca atatttccgg caatttcggc
     6421 cactggtatc cacgcccctc ttccggcaac ggataactgc attccctgtg gggcaacccc
     6481 cataaccaaa gcacctgctg ccaacatgct actttcagct ttcttcacca tctcaggcgt
     6541 atagaccgga tctgtgacct cactgcccaa ggcgctacta ggccagggtt taatatcaac
     6601 agaagggagc tcgatcggct taatctccac tggatcaata tcagcaggtt taaaatcagc
     6661 taattccagc tcgattgtct taatattttc tagatcaagc gcctgaattg tcggaatctc
     6721 cgggatatga atatcaggtg gtggtaacac agggatcaac agttctgctt tccccggtag
     6781 cgtatcaggc tttccattat tagaaggctc aataagatct ggtttttctg gagcagtaag
     6841 tgaggttccc gattccagtc gtgatttttc aactgaaaga atatccacct ttttggagac
     6901 aatctcatat tcatgcagaa gatgattata tttcgtaatc agttcgttct gcgcatcaaa
     6961 caattcttta tttggtaata ctaccatttc tttccatgaa gtgatgctcc agcgtgtatc
     7021 cggtcttgat gcgaggccaa taataaacgc cccttcaaaa taagagaaat cacccaattg
     7081 tttcgattta tcaagaaatt ccttatattt aaacttatcc gtaaattgat taagcatcgt
     7141 ttcaacatta tgtcgacgct catcaactac cacaattccg gaatttacat cctcaatact
     7201 tttttcaaga cgacttaatg ccgtatccaa ctcattaacc aagacagtaa aactgctgtt
     7261 tccacgaact tcaccaagaa gcgttttaac gctagataca a
//
