LOCUS       NZ_CP067428             5090 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli O22:H8 strain 154 plasmid p5, complete sequence.
ACCESSION   NZ_CP067428
VERSION     NZ_CP067428.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17217524
            Assembly: GCF_023518355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O22:H8
  ORGANISM  Escherichia coli O22:H8
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Marques da Silva,W., Bono,J., Amadio,A.F. and Cataldi,A.
  TITLE     Whole-genome sequence of Shiga Toxin-Producing Escherichia coli
            O22:H8 strain 154 isolated from cattle in Argentina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Marques da Silva,W., Bono,J., Amadio,A.F. and Cataldi,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2021) EEA Rafaela, Instituto Nacional de
            Tecnologia Agropecuaria, Ruta 34 Km 227, Rafaela, Santa Fe 2300,
            Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023518355.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 05:46:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,714
            CDSs (total)                      :: 5,585
            Genes (coding)                    :: 5,388
            CDSs (with protein)               :: 5,388
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 79 of 197
            Pseudo Genes (incomplete)         :: 128 of 197
            Pseudo Genes (internal stop)      :: 46 of 197
            Pseudo Genes (multiple problems)  :: 51 of 197
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067428.1.
            Bacteria and source DNA available from Angel Cataldi - Instituto de
            Biotecnologia - INTA Castelar, Buenos Aires, Argentina.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Escherichia coli O22:H8"
                     /mol_type="genomic DNA"
                     /strain="154"
                     /serotype="O22:H8"
                     /isolation_source="Rectal swab of live bovine cattle"
                     /db_xref="taxon:2491679"
                     /plasmid="p5"
                     /geo_loc_name="Argentina: San Miguel del Monte, Buenos
                     aires province"
                     /lat_lon="35.45 S 58.75 W"
                     /collection_date="2016-09"
                     /collected_by="Luisina Martorelli"
                     /genotype="stx1-stx2d"
     gene            join(4437..5090,1..429)
                     /locus_tag="JI426_RS27245"
     CDS             join(4437..5090,1..429)
                     /locus_tag="JI426_RS27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_249869009.1"
                     /translation="MNIKYILPLVSSFIISGCDSDGISKVKSSIDYSIDDSMTIGKAFD
                     TRSDCINGTWREEEDSRGRTVVTYTCDISETGIKIINSEIIKGPESSLTGSIKNNNMEI
                     DNINKSIEMYKQQASLIEQTRKLMIEHVRKLENAFNEDPKISNNINIEPYLNKMLNIEC
                     HQRNEGYSCTTDEECRPYMNGLFSPSVSYDYLEESCQKHILPVYQSYIKNVLPLKSKIK
                     AISFTGMFNYCGRSSEECIKLIDDKFNGFSSYNQKQTQKISFYEDLNTRKNKTLNECKK
                     ILNISDVKLKYYWRVTDTGGVDYLSGELTYRINGENKSVAYNNNLVKYAYVNYTEKQIP
                     SDYVNSILNQNYETIKNCSY"
                     /name="hypothetical protein"
     gene            complement(607..2157)
                     /locus_tag="JI426_RS27250"
                     /old_locus_tag="JI426_005373"
     CDS             complement(607..2157)
                     /locus_tag="JI426_RS27250"
                     /old_locus_tag="JI426_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_097464948.1"
                     /translation="MASYHCTVKTGAKGSALKHADYISRTGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERIHDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGPVQAA
                     SLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPELLLQYR
                     KVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARYLDKLGR
                     NVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHERNEFRNKL
                     QQAESEWDKENAAFKRTEGYKYVGDLDRYREREILAAASLENTRQKVAEEVSVARSQML
                     SLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALRIQRSWAREASRSNERDQGMER"
                     /name="MobA/MobL family protein"
     gene            2329..2775
                     /locus_tag="JI426_RS27255"
                     /old_locus_tag="JI426_005374"
     CDS             2329..2775
                     /locus_tag="JI426_RS27255"
                     /old_locus_tag="JI426_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_000184721.1"
                     /translation="MTNREDWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLTKDSA"
                     /name="conjugal transfer protein TraD"
     gene            complement(2969..3904)
                     /locus_tag="JI426_RS27260"
                     /old_locus_tag="JI426_005375"
     CDS             complement(2969..3904)
                     /locus_tag="JI426_RS27260"
                     /old_locus_tag="JI426_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_097464947.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSL
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLDNGYSQKAIAAMLNVHRNTIRNWAMHK"
                     /name="replication initiation protein"
     Typing          complement(2970..3904)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.411"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(611..2157)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.664"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctaaaatta aagcaataag ttttacaggt atgtttaact attgtgggag aagctctgag
       61 gaatgcataa aattaataga cgataaattt aatggctttt catcttacaa tcaaaaacaa
      121 actcaaaaaa tatcattcta cgaagatcta aacacaagaa aaaacaaaac attaaatgaa
      181 tgcaagaaga tattaaatat ctcggatgtt aaactaaaat attactggcg cgtaacagac
      241 accggaggtg ttgactatct gagtggggag ctaacataca gaattaacgg ggaaaacaaa
      301 tccgtagcat acaataacaa cctagtaaaa tatgcatatg ttaactacac agaaaaacaa
      361 ataccatcag attatgtgaa ttcaatactt aaccaaaact atgagacaat aaagaactgc
      421 agctattaat accacatctc acttactggc tgtttcacat ataacgcttg ttaatttcat
      481 taacacctga acagtgagtg aatcaaaacg ataaattttc tcattcagaa acgcttcacg
      541 ggacacggaa ttttttcgcg tcccgtgaag cgttggcggg gttcggggcg cagtcccgat
      601 attgcttcaa cgctccatac cttgatcacg ttcgttacta cgtgatgcct ccctggccca
      661 ggatcgctgt atacgcagtg ccttttcctc ctgttgctgc cgttcctgcg ccatttctgc
      721 cagtaactca tgtcgcattt gagctttttc atctccagat atactgagtt caggctccag
      781 agataacatc tggctacggg caacggatac ttcttcagca accttttgcc tggtattctc
      841 taaagatgcc gcggccagaa tctcacgttc tctgtacctg tccagatccc caacgtactt
      901 atatccctcg gtacgcttaa aagcagcgtt ttctttgtcc cactcggatt cagcttgctg
      961 tagcttgttc ctgaattcat tcctttcatg ttcagcggtt tccagcacac ttttttgctc
     1021 cagaatacgc gcatcaatct cccttttgtc agccccccga aacaggccac gaatcccatt
     1081 caaagcattg cgttgttcct gtagctcccg cagctccctt tgtgcggaaa cgacattacg
     1141 ccccagtttg tctaaatacc gtgctccttc atatattcgc ctgctccatt cggaaagaga
     1201 gtctttttta gctttcgcat tctgaagcag cttatgttct ttgagcgcat tgggattggc
     1261 atcccctaaa cgagcaagcc ttgcctctcc ctgaatgacc gttttcataa ccttgcggta
     1321 ctgaagcaat aactccggtg aacgggctga acgaacaggc tcaactgcct tgattttttg
     1381 cttttgctcc tgttcctgct gaatggcatt tgcggcatct ctggcggtct tgagttcccg
     1441 ctcggcattc ctccgttcct ggattgcaac aatctgctca ccattcagac tggcggcctg
     1501 aacggggcca agatgaactt ctggcgtgcg attgataccc tgttctttca gcgaacgatg
     1561 atctacgcgc gactcgtgac cgtgtcgcgc aaggtgctca ttctgcacat cagcccagcg
     1621 ggaacgcagc gcaacaaggg cggcttttcg ctctccggcg gtctggggaa tactcgcttt
     1681 ttttgcgcct ccccgctctg gtgatttgct gttggcacgt ttaaaaaatt gctgcggatc
     1741 gcgttcaata ccatcatgaa tccgctcaca aaacatgata tgcgcgtgtg gctgttcctt
     1801 tccgtcgatt gcacctggcg gattgtggat cgcgtactgg taggcatggc gatcgccaag
     1861 ttccttttgc acgaaatctt caacaagttc tatccgttgt tcgcgtgtca actcacgcgg
     1921 taatgcgatc tcaatttcac ggtatgccgt cccgtttttt cgttcaaatt catctgcggc
     1981 tttccatagc tctgcgggat ttttcttcgc ccatgatggc atgttaccag atgacgaaaa
     2041 ttccagatcc tcccgcgatt tgtagctctt atattcgccg gttctggaaa tgtaatcagc
     2101 atgttttaag gcactgcctt ttgctccagt tttgacggtg caatggtagc ttgccatcag
     2161 ccttttagcc ctccgcagga gcgcacagtt tctcgatgaa gtagagaaac tactaagtgc
     2221 gcatccctga ataaatcagg gatgatttta cgttctcaca cgcaggcaga caaggtgata
     2281 atatgcacaa taacaactca cattaacggc aaaagtgcag aggtaaggat gacaaatcgt
     2341 gaagactggt taagtgcaaa aattgcctac atcaacggac tgaaatcacc gtcagagcag
     2401 cagagattac ttgtgttact ggcagagaaa aaaaaccgaa caacgacgga tgaaaaaacg
     2461 ttgtccgcac tgatacgagc agaaaaaacc gcagagaaag cagccgcagc aaaagccaga
     2521 gtaacggcaa tcatagcggc agaacggaag gcagccgcaa gggcggaacg aaaggcacga
     2581 gatcatgaac tctacaaggc cgcaggcctc atgattgtgg caggactggt ggacagcaaa
     2641 accggaaaac caaagtttag tgctgccgaa ctggtaggcg cactcgcagg aatcgcagag
     2701 ctaccgcgca accatccaaa atggcaggaa tgggaaaaac gcgggaaaga gctactcacc
     2761 aaagacagtg cgtaagagcc tccgttcacg ttccggttcc ctggtgcttc ctcgctcatt
     2821 cgctcgctac gctcggtcat gagactgcgg cgagcacgac cacctcaatc atacggtaag
     2881 ccgcaccatt gctgggctga aaggagaaaa ccgccggaaa gcccgctgct caaaaagacg
     2941 ccgaaaaggc gctgttatct gatataggct atttgtgcat tgcccaatta cggatcgtat
     3001 tacgatgtac attcagcatc gccgcaatag ctttctgaga atatccatta tccagcatac
     3061 atagcgccat gaaacgcttg tcatcgtagg cttcgccttt agcgatacca cctttccggc
     3121 ctctcttggc ctgtatttct ggtgtgtgcg tatcagccac atactgtgcg aaagtttccg
     3181 gcgtgaagtt cctgtgcgtg tatttcgcaa tgctcttgcc aatagcccga cattcaggca
     3241 gagaaagcgg aacgggaagc gatgcgttgt acatttcgac acgctgaatc acggcatcaa
     3301 gccactgtga gaatgctggc cagccctgac gaatcgccct gtaagcccat ttgcgcgtca
     3361 tttcgaacag gtggcagttt cgccccatcc cgtaatgttt atcgatgcta cgacgctctg
     3421 aggcgctcaa atcgagataa tctgccagtt catcgagggt ataggcttcc tcgcgccact
     3481 ccatcacctg ccattccagg tggaacggat ttttgcaaat cagtccgctg tagttcacat
     3541 cagcacaaag tttctcacac agcgaacgtt caatcgcggc ggcatatttc agcgccttaa
     3601 ccgagctgtc cggcgcggtt ctcacggcga tattgagtgc gtagagcagg tgagcatggc
     3661 cgttcacagg attttttacg gtgatgttcg gcgcgggggc gttccggtcg ctccagtcaa
     3721 tcgcagctcc tacacggtca acgtcaaaaa ccagccagaa ctgcgcatga ggctggttct
     3781 gctggatgta ccgcgcaaga agcgcacgcc ctttgccgga gatgcgaaga ccaaaagcga
     3841 gatcatccgt gtgataggga cggtggggta aattttcgtc gaagtattga agcgcggcgc
     3901 tcatgtgtta cgattccttc caggttatcg accgacatcg cccctgtatt ttcgtgaaag
     3961 tcttggcgga tagacacgaa tttcaggggc gtttgttttt cagtcgctta cactaccccc
     4021 aacaacaaaa cctgtcgagc cttcccgcaa aaccgatcca tttaccataa ctcccgttgt
     4081 acgtaccccg taaaacgccc tcaatcgctc tctgacgcaa aaaacgcaaa aagacaagga
     4141 aacaccaggg taaaacagaa aaatgcgtca gaattgtttt cattggcctg cataactatg
     4201 cacgaaagcg atgcgcgatc ggtttcgacc gcacaaagct gcgaacgggt caaaaagaca
     4261 aactttctca tccagagaac ggtaaacggg cgcgaaatct tttcgagctc aatgaggcgc
     4321 tgagcaccaa atagtaagtc ggtttatacg gtatgcatgg attccattaa catattgttt
     4381 gtattaacat atttgcgttc gttattgtca aataagtcaa aagtgaaaag atgaatatga
     4441 acatcaaata cattcttcca ttagtatctt catttattat ttcagggtgt gactctgatg
     4501 ggataagcaa agtaaaatct tccatagatt attccattga cgactcaatg actattggca
     4561 aggcttttga tacaaggagt gattgtatta atggcacatg gcgtgaagaa gaggatagtc
     4621 gaggaagaac tgttgttaca tatacttgtg acatcagtga aaccggcata aaaataataa
     4681 actcagaaat aataaaaggc cctgagagca gcctgacagg ctctataaaa aataacaata
     4741 tggaaataga caacatcaat aaatcaatag agatgtacaa acagcaagca tcattaattg
     4801 agcaaacaag aaaactcatg atcgaacacg taaggaagtt agaaaacgcc ttcaatgaag
     4861 atccaaaaat atctaacaat attaatattg aaccttacct caataaaatg ctaaacatag
     4921 aatgtcatca gagaaatgaa ggctatagct gcacaacaga tgaagagtgc aggccatata
     4981 tgaacggctt gttttcacca agtgtatcat atgattactt agaagaatca tgtcaaaaac
     5041 acatattacc agtataccaa tcatatataa aaaatgtatt acccctaaaa
//
