LOCUS       NZ_CP067427             3596 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Escherichia coli O22:H8 strain 154 plasmid p3, complete sequence.
ACCESSION   NZ_CP067427
VERSION     NZ_CP067427.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17217524
            Assembly: GCF_023518355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O22:H8
  ORGANISM  Escherichia coli O22:H8
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Marques da Silva,W., Bono,J., Amadio,A.F. and Cataldi,A.
  TITLE     Whole-genome sequence of Shiga Toxin-Producing Escherichia coli
            O22:H8 strain 154 isolated from cattle in Argentina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Marques da Silva,W., Bono,J., Amadio,A.F. and Cataldi,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2021) EEA Rafaela, Instituto Nacional de
            Tecnologia Agropecuaria, Ruta 34 Km 227, Rafaela, Santa Fe 2300,
            Argentina
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: HGAP v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023518355.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 05:46:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,714
            CDSs (total)                      :: 5,585
            Genes (coding)                    :: 5,388
            CDSs (with protein)               :: 5,388
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 79 of 197
            Pseudo Genes (incomplete)         :: 128 of 197
            Pseudo Genes (internal stop)      :: 46 of 197
            Pseudo Genes (multiple problems)  :: 51 of 197
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067427.1.
            Bacteria and source DNA available from Angel Cataldi - Instituto de
            Biotecnologia - INTA Castelar, Buenos Aires, Argentina.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Escherichia coli O22:H8"
                     /mol_type="genomic DNA"
                     /strain="154"
                     /serotype="O22:H8"
                     /isolation_source="Rectal swab of live bovine cattle"
                     /db_xref="taxon:2491679"
                     /plasmid="p3"
                     /geo_loc_name="Argentina: San Miguel del Monte, Buenos
                     aires province"
                     /lat_lon="35.45 S 58.75 W"
                     /collection_date="2016-09"
                     /collected_by="Luisina Martorelli"
                     /genotype="stx1-stx2d"
     gene            complement(123..446)
                     /gene="mobA"
                     /locus_tag="JI426_RS27225"
                     /old_locus_tag="JI426_005370"
     CDS             complement(123..446)
                     /gene="mobA"
                     /locus_tag="JI426_RS27225"
                     /old_locus_tag="JI426_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            718..912
                     /locus_tag="JI426_RS27230"
                     /old_locus_tag="JI426_005371"
     CDS             718..912
                     /locus_tag="JI426_RS27230"
                     /old_locus_tag="JI426_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041642.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAESGTGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1408..1566
                     /locus_tag="JI426_RS27235"
     CDS             1408..1566
                     /locus_tag="JI426_RS27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071532985.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPKRHDLSPLAAATGNWNSRAMIM
                     RSVLQSS"
                     /name="replication initiation protein"
     gene            complement(1771..3288)
                     /locus_tag="JI426_RS27240"
                     /old_locus_tag="JI426_005372"
     CDS             complement(1771..3288)
                     /locus_tag="JI426_RS27240"
                     /old_locus_tag="JI426_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_000739598.1"
                     /translation="MKKNIVRQVLESYGPCISSELAERIKRQNPSMSSDAIRKMISRST
                     DIGKLPFLRFSHNRRFIYLKDDFGSFKFWRALKKCMREANSAYSHAILSVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYESVLKNLLSAKILRSVYIDGVGDCVLINNNIANDISIFNM
                     ANCESFFDKPIFELIKAWLRNLGIVAFNQIKTKYDGENNPVVGSFEWDITAPSYVSPLA
                     EYSSDGLIPGFVVCDFALGFNRNEITTDAADTFIRKVQMTKSSRTNQRIMFVLFARRFE
                     KNAFNKLRSEGVLAITIANAFGNKVDESLARLSKVIQGTLSIERHPDELLQMVKDLESI
                     SGENGNLRGYIFELFVSSQICNFYGYGNVRINKEYKINGKHAEADVVLETNDDIYIVEC
                     KNVKTLPSMEVSLWMKTRVPIINSYYKSNNPDNKNIHHRLWVTGNIYPKDINRLDKFKC
                     NNKKIDVDYLFGQSLDDFFYQKKQVFNLYRKVISPDYKKGQIPDFDL"
                     /name="NERD domain-containing protein"
     Typing          complement(456..680)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.167"
                     /coverage="100.0"
                     /evalue="4.85e-75"
     Typing          958..1476
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(127..446)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="8.379999999999999e-60"
ORIGIN
        1 ggaaggatgc gtcaggatcg ttttcagctc gctgcatagt tatgcatgaa agcgaatggt
       61 gatcactttg ggagcttacg gtgttcatac cgtctgtttt cgacagtttt ctccccggga
      121 agctaatctg ccataagcct ggataacagg gcacggtgat actccgtaat agcgatcagc
      181 acctccgcat actccctgtc cccgacacgc ttgccgtcga taaagagttt tttctgcaac
      241 gcccccagtc gcatgacttc tttcagcacc cggtcatcag tcagtgagtt aactttctta
      301 ccgagagctg ccgcccgtaa aaaaccagaa acggtcttgc cgcattcagc agcccttttt
      361 ctgatttcct ggtcttcttc tgccgtcaga cgaacagggc gactgatagc ccgcctgcgc
      421 gtattacttc cacttcgttt tgtcataaaa catggctccg tatctgacac ggggtgtcgg
      481 ggcgaagccc tgaccagggt aattgtaata gcgtgcatgc atgcgcggta taacaattgc
      541 acatcctgtc ctgtctgcaa gttcgaatta ccaacaaagc actgctgttt tttggcagtg
      601 acagtgttgt ctgactgaaa tgtccgtcgg cagaataccg gcggtaagac ggtcgaaaag
      661 tggtacgcga tataatcaca cgacgcagag gacagacaga acggagaaaa cagaaagatg
      721 aacaagcagc agcaaaccgt actcaacatg gcgggattca taaaaagcca gagcctgacg
      781 ctgctcgaaa agctggacgc actcgatgcc gacgaacagg ccgccatgtg tgagaagctg
      841 cacgaactcg cggaagaact ccagaacagc atacagacac gctttgaagc ggaaagtggc
      901 accggggtat aacagtgtat cctgctccag acaggatgca gaacggtata ccggtacgta
      961 aggagaaaat gccacataag gcgctcttcc gcttcctcgc tcactaactc gctacgctcg
     1021 gtcgttcggc tgcggcgagc ggtatcagct cactcaaaga cggtaatgcg gttatccaca
     1081 gaatcagggg ataaagccaa aagaaacatg tgagcaaaaa acaagaaccc gaaaaaggcc
     1141 gcgccggtgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1201 tcaagccaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1261 agctccctcg tgcgctctcc tgttccgacc ctgccgttta ccggatacct gtccgccttt
     1321 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gttggtatct cggcccggtg
     1381 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1441 gccttatccg gtaactatcg tcttgagtcc aacccgaaaa gacacgactt atcgccactg
     1501 gcagcagcca ctggtaactg gaatagcaga gcgatgataa tgcggtccgt actacagagt
     1561 tcttgaagtg atgccccaac tacggctaca cttaaaggac aggatttggt atctgcgctc
     1621 tgctgaagcc agttacctag gttaggtaat ctgccaggat taactaacct tcgaaaaacc
     1681 accttgccgg gtggtttttt cgtttcatgg aggaagatac gcttttcaag ccgtcatcgt
     1741 caagaaatgc agagactaga aaacgtcatt ctataagtca aaatcgggta tttgcccttt
     1801 tttataatct ggcgaaatga ctttcctata taaattaaac acttgttttt tttggtagaa
     1861 aaaatcatcc aaagactgac caaataaata atcaacatca atcttcttat tattacattt
     1921 aaacttgtcc aatcggttta tatctttagg atatatattc cccgtaaccc acaaacgatg
     1981 atgtatattt ttgttatcag ggttatttga tttgtagtag gaatttataa ttggcactct
     2041 tgttttcatc caaagactaa cctccatcga aggtaaagtc ttaacatttt tgcactcaac
     2101 gatataaata tcatcgttag tttccaatac aacatcagct tcggcatgtt taccgtttat
     2161 tttatattct ttattaatac gaacatttcc ataaccatag aaattacaaa tttgagagga
     2221 aacaaaaagc tcaaaaatat acccccttaa atttccattc tctccagaga tagactccag
     2281 atcttttacc atttgaagta actcatcagg atgtctttca atagataaag ttccctgaat
     2341 caccttcgat agtctggcca agctctcatc gaccttatta ccaaacgcat tagctatagt
     2401 tatagccaga accccttcac tccttagctt gttgaaggca ttcttctcaa aacgtctagc
     2461 aaaaagaaca aacataatcc tctggtttgt cctagaggac ttggtcatct gaacttttct
     2521 tatgaaggta tcagcagcat cggtggtaat ttcatttcga ttaaacccta gagcaaaatc
     2581 acagacaaca aaacctggga tgagtccatc gctggaatat tcagcaagag gggacacata
     2641 acttggcgct gttatatccc actcgaaaga accaaccaca ggattgttct ctccatcata
     2701 ttttgttttt atttgattaa aagccacaat acctaaattt cgcaaccatg ccttaatcag
     2761 ttcaaatatt ggcttatcaa aaaaagactc gcaatttgcc atattaaaga tgctgatatc
     2821 attagctata ttattattta tcaaaacaca atcaccaaca ccatcaatat acacagatct
     2881 aagtatttta gctgaaagca gattttttag aacagattca taacttaaat gcttggcctg
     2941 ctttatcgga gagccagaca caatcccgaa atcctttacc ttcaagtatc cgccattatt
     3001 aattacggaa agtatagcgt gagaatacgc cgaatttgcc tcacgcatac attttttgag
     3061 cgcccgccaa aatttgaagc taccaaagtc atcttttaga taaatgaatc tcctgttatg
     3121 actaaatctt aaaaatggca acttaccaat atcagtgctt cgagagatca ttttacgtat
     3181 tgcatcagat gacatagaag gattctgacg cttaatcctc tcagcaagtt cagaagatat
     3241 gcacggacca taactctcaa gcacttgtct aacaatgttt tttttcataa attcatctta
     3301 tttttgtcac actacgctat ttataaaatt acactacgtg taatatctat agatccccaa
     3361 aaaaacacaa agaaaaaaat cataaaaatc aaatatttaa caaaagcaac atatcacaaa
     3421 aaacacacct gcgacattta tccttaaaaa tcaagatgtt acttttgtga catttcatca
     3481 tcatatatca accgccctat ttaccataat cccttaattg tacgcaccac tgaaacgcgc
     3541 tgaacgcgat tctgacgcag acaccgcaaa aagagatgtt ttaccccgga aaaata
//
