LOCUS       NZ_CP067387             3335 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Klebsiella grimontii strain 5512.56 plasmid p5512.56-5, complete
            sequence.
ACCESSION   NZ_CP067387
VERSION     NZ_CP067387.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17215154
            Assembly: GCF_017863935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Campos-Madueno,E.I., Moser,A.I., Risch,M., Bodmer,T. and
            Endimiani,A.
  TITLE     Exploring the Global Spread of Klebsiella grimontii Isolates
            Possessing blaVIM-1 and mcr-9
  JOURNAL   Antimicrob Agents Chemother 65 (9), e0072421 (2021)
   PUBMED   34181480
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 262.28x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017863935.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:04:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,919
            CDSs (total)                      :: 5,803
            Genes (coding)                    :: 5,650
            CDSs (with protein)               :: 5,650
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 71 of 153
            Pseudo Genes (incomplete)         :: 96 of 153
            Pseudo Genes (internal stop)      :: 26 of 153
            Pseudo Genes (multiple problems)  :: 34 of 153
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067387.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="5512.56"
                     /isolation_source="Not defined"
                     /host="Homo sapiens"
                     /db_xref="taxon:2058152"
                     /plasmid="p5512.56-5"
                     /geo_loc_name="Switzerland"
                     /lat_lon="47.38824 N 8.34350 E"
                     /collection_date="2014-09"
                     /collected_by="Institute for Infectious Diseases,
                     University of Bern, Friedbuehlstrasse 51, 3001 Bern,
                     Switzerland"
     gene            complement(join(2718..3335,1..117))
                     /locus_tag="JJJ11_RS00590"
                     /old_locus_tag="JJJ11_00590"
     CDS             complement(join(2718..3335,1..117))
                     /locus_tag="JJJ11_RS00590"
                     /old_locus_tag="JJJ11_00590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_130953829.1"
                     /translation="MALMVTRRYLLGGEYPSEEFVQASLEKYFFRQGFDIDTGSYIDLI
                     CRDKESRDVVWHIEVKGKTSQPGLDFRTCLGQLVQRMTKDNINYAIAVPRIKQYERLIE
                     ETSILAVSKLNLHWIFVGPDGSVTIKEPDSSTMKNGNGNGNGNGREVSLVNGGFKRFIT
                     GDFMPTCSYGKAPVVFKSSRREMWDWLDDFVISNGVIPSIADIKKEAESRSGKWTLSNV
                     KIEYQFWKIYHLYKGHFFYQSE"
                     /name="hypothetical protein"
     gene            complement(317..487)
                     /locus_tag="JJJ11_RS00595"
                     /old_locus_tag="JJJ11_00595"
     CDS             complement(317..487)
                     /locus_tag="JJJ11_RS00595"
                     /old_locus_tag="JJJ11_00595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157771094.1"
                     /translation="MATATNFDAWLDDVDGDYEEVMALYDSVQNVSDMGLYQCVEGGRG
                     DAWVVSSNHHP"
                     /name="hypothetical protein"
     gene            complement(1338..1739)
                     /locus_tag="JJJ11_RS00600"
                     /old_locus_tag="JJJ11_00600"
     CDS             complement(1338..1739)
                     /locus_tag="JJJ11_RS00600"
                     /old_locus_tag="JJJ11_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_048270303.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNILLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1739..1930)
                     /locus_tag="JJJ11_RS00605"
                     /old_locus_tag="JJJ11_00605"
     CDS             complement(1739..1930)
                     /locus_tag="JJJ11_RS00605"
                     /old_locus_tag="JJJ11_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_049139654.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEELHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2364..>2444
                     /locus_tag="JJJ11_RS29505"
                     /pseudo=""
     CDS             2364..>2444
                     /locus_tag="JJJ11_RS29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          2107..2491
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.487"
                     /coverage="89.0"
                     /evalue="2.97e-154"
ORIGIN
        1 acccgtatct atatcgaacc cttgtcgaaa aaaatatttt tctaaagaag cttgaacaaa
       61 ttcctcggag ggatattcac cacctagtaa gtaccttctc gtcaccataa gagccacaac
      121 ccctacgcat cctatttact ataacgtcat attttaagag ttcccacaag gggaactctc
      181 tgtacgatta cgagtgatca ttcgatatat ttcgttgaaa tccataccat gaatcaacat
      241 cttcgccgcc acatagcctt tctctaatca atttgaggaa agtatcccta gccacagctg
      301 atgctagaaa aagagcttat ggatgatgat tagagctcac gacccaagca tcaccccgac
      361 caccctcaac acactgatac aaccccatat cacttacatt ctgaacggaa tcataaagag
      421 ccataacctc ctcataatcg ccatcaacat catctaacca cgcatcaaaa ttagtagcag
      481 tcgccatgcc aatcctccat tttttagttt tcaggagtta atttacatag tgataggggc
      541 tgttaagcaa ataagttttt accttttttg cctcatgctc taaaagtgag caagacaggc
      601 atttaccata acgtcccatt gtacgcaccg ctaaaacggc ctcagggcgt ttctgacggg
      661 aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt
      721 tttacggcat gcatgagtat gcacgcaagt gcatagcgcg aaaattcacg ccattcagcc
      781 agggtaagtc ctgtttccgg cgtacttccc atccgaaaac ggtgaacggg gacgcaattt
      841 attgcgtggc cgtgaagcgt tgccggcgca gccggaaaac cggttttcag cacgttcagg
      901 aatccggttc gtcgacggcc ggcaatcacg ttatggcaag tcaccgaacg actcaccaca
      961 cgattcaccg gcgactcttc actagagtcg tacccagagt cgccacgcct aaaaaacaac
     1021 caaacgggga gtcgggtcta gagtcgggtc gttattaact cattgttttt atagtcctga
     1081 gtcgttaccg actctatgac gactcgccgg ggactctatg acgagtcgtc atagagtcgg
     1141 atctatataa atcaaattta aaaaacgaaa ttttacagac ttaaactact gatattaaat
     1201 acaaatccgc aaaattttaa ttttgcgtag tgtgtggata tttttagtgg tgaggagcgt
     1261 tcagaaatcc ggcgattttt tggaaggttc aggaactccc cgggatggcc ggagaggaaa
     1321 acggaagcat gacggggtca ggattttttc cagaatttcc accatggcgc cggggattca
     1381 gttaattttg cctggtgatt ttcaagaagg agcatggcct gtttcaggct gtcaatgtgc
     1441 gaatccttgg acaaaagtaa gatattcagg tgttcaatct cctgtttcag atgacgaatc
     1501 acctgttcac tctggtcagc agaggctgta tcacgctgtt cggagacctg tccagaatgg
     1561 tgttcaccct tcggttgctc aaagggacca aagacacgta aaagctctga agtatctacc
     1621 atttttgcat tgcgctcacc actacaggtg gacaggtcac ctgttttaat taacctgtgc
     1681 agtgttgttc tactcttacc cgtcagtcgg gcagcctcac taatagacac tctggccatc
     1741 agttaccatc ctgtaatgca tccagtctcg aggacagtgt acggaaaaga tgctccgcat
     1801 catcgtggag ttcctcacaa aggtcggcct cgacatcgag atcaagctcg ttaagcttct
     1861 ccagcaggag cagagactga ttctggatga atttagccat gttcagcgct gttttctgtt
     1921 gtttcgtcat aagaggcctc agagcgtcag tgctcaatgt agggatgttc tcttttccgc
     1981 ttcctcgctc actcagtcgc tacgctcctg ccgttcggct gcggcgagcg gcaggagcat
     2041 gcgtataaaa gctcgagaaa accgagcgat cgcgtgatgg ggagaaggag aggccgcaca
     2101 gcggccgttt ttccataggc tccgcccccc ctgacaagca tcacagaaac tgacgctcaa
     2161 atcagtggtg gcgaaacccg acaggactat aaagatatca ggcgtttccc cctgatggct
     2221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccag atacctgtcc gcctttctcc
     2281 cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcagt tcggtgtagg
     2341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgactaatcg ccattgacaa
     2461 cggccactgg tagacaggat gtgcgagaga tgtatcgaga gttcttgaag tggtggcctg
     2521 actgcggcta cactgaaagg acagatttgg tgacatgtct cgcacaagac tgttaccacg
     2581 gttcagaagt tccccaactt actgaacctt cgaaaaacca cctccccagg tggttttttc
     2641 gttttccgag aaagagatta cgctttgagc gaatgaatct caagaagatc atggcatttg
     2701 atgaacaaat gagtaagcta ctcagattga tagaaaaaat gccccttata caagtgatat
     2761 attttccaaa actgatactc tattttaaca ttagaaagcg tccatttccc actccgcgat
     2821 tcagcctctt ttttaatatc agcaatactt gggatcaccc catttgaaat aacaaaatcg
     2881 tccaaccaat cccacatttc tcgccgagat gatttaaata caacaggagc tttcccatat
     2941 gagcaagttg gcataaaatc ccccgtaata aatcttttaa accccccatt cacaagagaa
     3001 acttctcttc cattaccatt accattacca ttaccatttt tcatcgtaga gctatcaggt
     3061 tccttaattg tcacactgcc atcaggccca acaaatatcc agtgtaaatt caatttacta
     3121 acagcaagaa tgctcgtttc ttctattaat ctttcatact gttttattct tggaacagca
     3181 atagcataat taatattatc ttttgtcatt ctctgcacta attgtccaag acaagttcga
     3241 aaatccaacc ccggctgtga tgtttttcct tttacttcaa tatgccacac aacatcccta
     3301 gattctttat cacgacaaat aaggtcaata taaga
//
