LOCUS       NZ_CP067374             3813 bp    DNA     linear   CON 16-JUN-2024
DEFINITION  Klebsiella pneumoniae strain BA30371 plasmid p_unnamed.
ACCESSION   NZ_CP067374
VERSION     NZ_CP067374.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214426
            Assembly: GCF_023518335.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Hybrid assembly of VBA30371 Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. August 2020
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75.9x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023518335.1-RS_2024_06_15
            Annotation Date                   :: 06/15/2024 05:07:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,385
            CDSs (total)                      :: 5,261
            Genes (coding)                    :: 5,124
            CDSs (with protein)               :: 5,124
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 60 of 137
            Pseudo Genes (incomplete)         :: 76 of 137
            Pseudo Genes (internal stop)      :: 25 of 137
            Pseudo Genes (multiple problems)  :: 20 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067374.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA30371"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p_unnamed"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2019"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College, Vellore"
     gene            complement(1124..1441)
                     /locus_tag="JI970_RS26850"
                     /old_locus_tag="JI970_26825"
     CDS             complement(1124..1441)
                     /locus_tag="JI970_RS26850"
                     /old_locus_tag="JI970_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225865423.1"
                     /translation="MVDLLAVDVCSYGEIVATDAQVKQINALIKKYPDSCSICHQEYDE
                     DYVTYTVFGYDKNQKMQVTTGCCARTLTELVLLGVCGCFDPDERDEIMENHPMAKKFFS
                     N"
                     /name="hypothetical protein"
     gene            complement(2197..2598)
                     /locus_tag="JI970_RS26855"
                     /old_locus_tag="JI970_26830"
     CDS             complement(2197..2598)
                     /locus_tag="JI970_RS26855"
                     /old_locus_tag="JI970_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_070585248.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESQAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2598..2789)
                     /locus_tag="JI970_RS26860"
                     /old_locus_tag="JI970_26835"
     CDS             complement(2598..2789)
                     /locus_tag="JI970_RS26860"
                     /old_locus_tag="JI970_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_070585250.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDGLQDDN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3412..3626)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.105"
                     /coverage="100.0"
                     /evalue="7.539999999999999e-48"
     Typing          2..460
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.174"
                     /coverage="93.0"
                     /evalue="5.14e-98"
     Typing          3601..3813
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.785"
                     /coverage="97.0"
                     /evalue="8.98e-66"
ORIGIN
        1 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggtg gctccctctt
       61 gcgctctcct gttcccgtcc tgcggcatcc gtgtagtggt ggaagcttta cctaaatcac
      121 cacgccccgt tccgtttaga cagttcgctc caagctgggc tgtatgcaag aaccccccgt
      181 tcagcccgac cgctgcgcct tttccggtaa ctatcgtctt gagtccaacc cggaaagaca
      241 cgacttaacg ccactggcag cggccactgg taaacaggat gtgcgagaga tttacgaaga
      301 gttcttgaag tggtggccag actgaggcta cactaaaagg acagatttgg tgacgtctcg
      361 cacaagactg ttaccacggt tcagaaactt gccggtttac tgaaccttcg aaaaaccacc
      421 ctgcaaggtg gttttttcgt ttacatggca agagactacg acgtccaacg tcagatctca
      481 agaaattatt taaaaataaa tagttaacca atatccttgt gatatttatt atgtttcctt
      541 tatactcaaa taacatcaat attcaggatt ggaacactca aacaagttaa tacaacgatg
      601 ttctaggttc atatccctga gacttaaagt ctaactagta tatttccgaa caagatcact
      661 atcattccgt acttttttaa ataggtttat ttaatgccat acatatacgc aataatacta
      721 acaacttgtt ttaacacgaa taatattgca agttgttctt cagaattagc aaatttggga
      781 atgaaagaca atgttgttaa caccttcatt ctgaatgaag aatgcattga atttaaagat
      841 aaaatcattg caaacaaaga catttatcat aatatatccg ggaaaaacat aatatctgct
      901 gaatgtgtac ccgtatctat ccccaaatat attttagaca attacagcaa acaacaacga
      961 taatgaatac cccctctgca agcagcgggg tatcaacacc aaagatcaaa tacagagtag
     1021 ttcgctccaa gggacgggga atattgcctt tagagattta ccataacgtc tcattgcacg
     1081 aacccaaacg cccgcaaaga gcgggcttcg gtaatagtca ggtctagttg ctgaaaaatt
     1141 tcttcgccat tgggtgattt tccatgatct cgtcacgctc atctggatca aagcatccgc
     1201 aaacacctaa caacaccaat tcggtaagcg ttcgcgcgca acatccagtt gtaacctgca
     1261 tcttctggtt tttgtcatat ccgaaaaccg tatacgtaac gtaatcctca tcgtattcct
     1321 gatggcaaat agagcacgag tcagggtatt ttttgatgag cgcattaatc tgttttactt
     1381 gtgcatctgt agccacaatc tctccataag aacaaacatc aacagccagc aaatctacca
     1441 catcagcttc gcgctaccga tttaccataa cgtcgcatta cgcgcacctc tcagacagcc
     1501 tgagagcgtt tctggcagaa aaccggtaaa acaggcaaca aaccacccga aaacccgcca
     1561 gagacgcgca ggcgctattt ttacggcatg catacgtatg cgcgcaagtg catagcccga
     1621 aaattcacgc cattcagcca gggtaagtcc tgtttccggc gttcttccca tccgaaaacg
     1681 gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt gccggcgcag ccggaaaacc
     1741 ggttttcagc acgatcagga atcgggttcg tcgacggccg gcaatcacgt tatggcaagt
     1801 cgacggacga cttaccacac gattcaccgg cgattcgact cgtgacttga ctctagagtc
     1861 gccaaggtca aaaaaacacc aaaatgacga ttcgactcta gagtcaagtc gtcataagtt
     1921 actgatagtc ttatgtgagt cgtcgatgac tcaatgacga gtcgtcggca agtcgtacac
     1981 gaatcgccga cgacttggat ttaccttaca gaaggtaata attcgtaatt taataacacc
     2041 taacaaactg atattaatga gttatctgca aaattttaat tttgcgtggt gtgtgggtat
     2101 tttaagtggt gaggtgcgtt cagagatctg acgatttttg ggaaggttca ggaactcccc
     2161 ggaatggccg gagaggaaaa ctgaagcatg acggtgtcag gattttttcc agaatttcca
     2221 ccatggcgcc tgggattcag ttaattttgc ctggtgattt tcaagaagga gcatggcctg
     2281 tttcaggctg tcaatgtgcg aatccttaga caaaagtaag gtgttcaggt gttcaatctc
     2341 ctgtttcaga tgacgaatca cctgttcact ctggtcagca gaggctgtat cacgctgttc
     2401 agagacctgt ccagaatggt gttcaccctt cagttgttca aagggaccaa agacacgtaa
     2461 aagctctgaa gtatctacca tttttgcatt acgttcaccg ctacaggtgg acagatcacc
     2521 tgctttaatt aacctgtgca gtgttgttct actcttaccc gtcagtcggg cagcctcact
     2581 aatagacact ctggccatca gttatcatcc tgtaatccat ccagtctcga ggacagtgta
     2641 cggaaaagat gctccgcatc atcgtggagt ttctcacaaa ggtccgcctc aacatcgaga
     2701 tcaagctcgt taagcttctc cagcaggagc agagactgat tctggatgaa tttagccatg
     2761 ttcagcgctg ttttctgttg tttcgtcata agggccctca gagcatcagt gctcagtgta
     2821 aaccggtaag ggggtcggat gtacgaatgg ttcagagaac ggatctgagg ttatcctgta
     2881 gttattttgt agtttatctg tagtcatcgc actaaaacgc aggattaaat catcaacttt
     2941 tgtagtcttc ctgtagttta cctgtagtct ggaaaaataa tccgttcatg cttgcatgaa
     3001 cgaagcagtt aaagacgctt tttcgtcgcc gatgatcctt aacctgcgca gatcacccag
     3061 ccagaatgat aattttttgc cccttaaatg gtcatacacc taacgcagcc gcgattttta
     3121 tcgcaactgc atgaattaaa tgaaaaaaat ggagttactt cgtgtgttag cgtaatacgc
     3181 aacgtattac aatcatctgt aacaacatga aaataaacaa ataacatgta ataccagcgt
     3241 attacatgta cgctggccag cgaaaaagct ataaagcttc ccggccgaca ggcgggccag
     3301 aatccaccgg ggcgtgcagg ttctcttcag taacatgagc cattaagtca aaaaggtcat
     3361 attcgccggg aaacagagca tcagtcgcat ccggttgttc atcatactca gatggcatgg
     3421 tgtgatatcc aatagctgag gggtgttagc gggtttcggg gcgcagccct gaaccagtca
     3481 cgtagcgcta gcggagtgca tactggctaa atattttaaa ccgaagtggt gatttgccaa
     3541 agtgcttcat gtggcaggag tgaaatgagg ctttccggcg tcaggagaag atgctggttc
     3601 aggtgatatt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3661 gcggtgcagg cttactaatg gggcggaaat ttatttccta gtggatgcca ggaagaaact
     3721 taacagggat acgataaggc cgcggcaaag ccgtttttcc ataggctccg ccccccctga
     3781 caagcatcac gaaatctgac gctcaaatca gtg
//
