LOCUS       NZ_CP067306             3849 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain ABW_A32 plasmid pABW_A32c, complete
            sequence.
ACCESSION   NZ_CP067306
VERSION     NZ_CP067306.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214838
            Assembly: GCF_017639145.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Biggel,M.
  TITLE     Characterization of fosA carrying plasmids from multidrug resistant
            Enterobacteriaceae food and environmental isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 03:33:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,599
            Genes (coding)                    :: 4,364
            CDSs (with protein)               :: 4,364
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 70 of 235
            Pseudo Genes (incomplete)         :: 164 of 235
            Pseudo Genes (internal stop)      :: 49 of 235
            Pseudo Genes (multiple problems)  :: 48 of 235
            Pseudo Genes (short protein)      :: 4 of 235
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067306.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ABW_A32"
                     /isolation_source="waste water"
                     /db_xref="taxon:562"
                     /plasmid="pABW_A32c"
                     /geo_loc_name="Switzerland: Basel"
                     /collection_date="2015"
                     /collected_by="ILS, University of Zurich"
     gene            join(3706..3849,1..465)
                     /locus_tag="JJB23_RS23220"
                     /old_locus_tag="JJB23_23215"
     CDS             join(3706..3849,1..465)
                     /locus_tag="JJB23_RS23220"
                     /old_locus_tag="JJB23_23215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063859057.1"
                     /translation="MAKCLLVKVRRETSVAVFRQCEIREKYLAIYKESFDEVITYLEKA
                     GALEPGVLRLTTIDDNAIRAWKSQWKGRSRKHAHGAWDWQNLVSKRARSCKRFDVAVWG
                     EDVLCGLSVGKLTRGKKTVRMDYLEACPTAHPLEKRITMIVVAVALSVAKKVGAQHVAI
                     FNPIKDKEEKVLKHYQSYGFTQRMLYGRFLKNVLYKEVV"
                     /name="hypothetical protein"
     gene            complement(469..639)
                     /locus_tag="JJB23_RS23225"
                     /old_locus_tag="JJB23_23220"
     CDS             complement(469..639)
                     /locus_tag="JJB23_RS23225"
                     /old_locus_tag="JJB23_23220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152924371.1"
                     /translation="MSYVSERKSELVKAQQRKMDPSSPDSAPINQGLMDFILLAMKVDD
                     GFVQRGRIHGA"
                     /name="hypothetical protein"
     gene            complement(912..1056)
                     /locus_tag="JJB23_RS23600"
                     /pseudo=""
     CDS             complement(912..1056)
                     /locus_tag="JJB23_RS23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1600..1800
                     /locus_tag="JJB23_RS23230"
                     /old_locus_tag="JJB23_23225"
     CDS             1600..1800
                     /locus_tag="JJB23_RS23230"
                     /old_locus_tag="JJB23_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_061585996.1"
                     /translation="MTDDRTLQLRLTGFRKAEASLRLEGMDPSGTPLYESVKTRILSGD
                     ITFDEGRTEIRAHYQKRANSN"
                     /name="antitoxin VbhA family protein"
     gene            2550..2876
                     /locus_tag="JJB23_RS23235"
                     /old_locus_tag="JJB23_23230"
     CDS             2550..2876
                     /locus_tag="JJB23_RS23235"
                     /old_locus_tag="JJB23_23230"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032431019.1"
                     /translation="MFGFFKKKKLYEEICKDAGMALSDGLLAQGLARNKIEAMGAGAVF
                     SQSLREAVSQGYKSSDAIAEARKNTSHHLAARGFDFETIASAIDVFCTATAFESMLDLA
                     RDKG"
                     /name="hypothetical protein"
     gene            complement(3002..3466)
                     /locus_tag="JJB23_RS23240"
                     /old_locus_tag="JJB23_23235"
     CDS             complement(3002..3466)
                     /locus_tag="JJB23_RS23240"
                     /old_locus_tag="JJB23_23235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061583928.1"
                     /translation="MEDKNNLSSEVKNHVSKWGKTNISAGWTIIPNALLENQSRLGLSC
                     IDTMVLINLIMHWWEKDNPPRPSKKRLANMLGVSLKTVQRSFIHLEQCGAIKRIPRYKE
                     GKDNARTTNHYDLNGLVDLLEGFSKELIEEREANRKSEVNRPKKRGNPKS"
                     /name="hypothetical protein"
     Typing          complement(949..1549)
                     /name="rep_cluster_2324"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.05"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 gagcccggcg ttttaaggct cactacaatc gatgacaatg ctattcgtgc atggaagagc
       61 caatggaaag gcaggtctcg taagcacgct catggtgctt gggattggca gaaccttgta
      121 tcaaagagag ctcgttcttg taaaagattt gatgtggctg tttggggaga ggatgtgctt
      181 tgtgggcttt cagttggaaa gctaacccgt ggtaaaaaga cagttagaat ggattatcta
      241 gaggcttgtc caacagctca tccgttggag aaacgaatca caatgattgt ggttgctgtg
      301 gctctgtcag tagctaaaaa agtaggggca cagcacgtag ccatattcaa cccgataaaa
      361 gacaaagaag aaaaagtgtt aaaacactac cagtcttacg ggtttacaca aagaatgttg
      421 tatggacgct ttttgaaaaa cgtgctgtac aaagaagttg tttgatagct atgcgccgtg
      481 aatacgacca cgttgaacaa agccgtcatc tactttcata gcaagcagga tgaagtccat
      541 aagaccctga ttgattggag ctgaatcagg agaggatgga tccattttgc gttgctgtgc
      601 ctttaccagc tcacttttgc gctcagatac ataactcatc ttgtgactct ctgtcttgta
      661 tggggatgta gacactataa caagtggata aaaaaataac gagatctcta tattttcaaa
      721 aagagatgac tgttgttgac tcgcctcttc tgcaaagcgt actcttttct ttcaaaagaa
      781 aaaaaccgcc tttgcggggc ggtttttttc cagttcattg agtttgcggc tctttgaacc
      841 taggtaacag tcttgtgcga gacgtgtcac caaatctgtc cttccagtgt agctgtagtc
      901 aggctttaac ttcaagaact ctcgatacac ctctcgcact cactgctacc aatggctacc
      961 gccagtggcg ctttgtcgtg tctttcaggg ttggattcaa gagtacagtt acctgataaa
     1021 gacgtggtag tcgggctgaa cggggagttc gtgcatacag cccagcttgg agcgaaccac
     1081 ctacaaggaa cccagttgca cgggtaggaa tgagaaaacg ccaccttacg gcggactgca
     1141 acgggtaaac ctcagggtgg gaacaggaga gcgcacgagg gagcgaccca tcggaaacgg
     1201 tggggatctt taagtcctgt caggtttcgc cacttctgat ttgagcgccg gaaatcacct
     1261 gatttctgtg aagcttgtca ggagggcgaa gcctatggaa aaccggcaat ccccagaacc
     1321 tccttatggc cttctgcctg agcaggtaac gctcgccgca gtgtcatgac cgaacgaagt
     1381 gagcgaatga ccgaggaagc gtaatgtctg taatgctgaa cagggatcgt gtcgctcggt
     1441 acacacgttt cctccctgat tacacacccg gctgcaacag tttcctgtcg ttttccactt
     1501 ttccctgcac agctatacaa cggcaacatc cgggcaggac atgtaaagtt agctaacttt
     1561 acgcctgcct tattcgcatg actgctcaaa ggaaaaacca tgactgatga tcggacactg
     1621 caactgcgcc tgacagggtt ccgtaaagcg gaggcctctc tcaggcttga agggatggat
     1681 ccatcaggaa ccccgcttta tgaatcggta aagacaagga ttctttccgg tgacatcacc
     1741 ttcgatgaag gccgcactga gatacgggct cattaccaga agcgggcgaa cagtaactga
     1801 cgcggacagg aaatgttttg tggccaccgc cgctgcgccg gcgccacaaa atggcgggac
     1861 aagatgtgtt tttggagtac cgccggcact ccagaatgtt tcatcaggcc ccgcccgata
     1921 ccctgaattt cgtacgtacg aaatttcgct gctgttcaga tcgagagcag gattcgcaaa
     1981 ttgctggcgt ttgtacccac tgtaaatctg ccgcgcctgg cggcttggcc aacgcttcac
     2041 gaacgcacag taaactgcgc actcgttcac cgttaccaga aaggccattg cgggaataat
     2101 tctttcagtc gttctacgag atcaagcagc tcattcatga ccttcagcat gacgcttatc
     2161 agtttcaaaa atttaggcat ataaaaattt cctgtacgga gttgggtctg gatcaggaaa
     2221 ttagggactt tgtaattttc agcggtagaa aaatcagttt ttctaaaaaa attttttagg
     2281 cggcgaaaag tgaagtaatg agcactgctt cgtgctgaaa atgaggctca ggcaggtgtt
     2341 ttgcagcgaa ggcgcgcgtc attcactctc gtgcatagcc atgcagcggc ctaaaaatca
     2401 tcaggggcgt ttttcaggag tatggacagg gaaaggtgcc ggaaacggcc cggacgcccc
     2461 aggatcgcgc tgaggggctc tcagggggtg cgggcaaggg atgttatggt aaattgctca
     2521 tcacagtcgc cagttaagca gaggttagaa tgtttgggtt ttttaagaaa aaaaagctct
     2581 atgaagagat ctgtaaagat gcagggatgg ctttatctga tgggttattg gcccaaggct
     2641 tagcgagaaa caaaatagaa gcaatggggg ctggtgctgt ttttagtcag tctttgaggg
     2701 aggctgtttc tcaaggttac aagtcttctg atgcgattgc tgaagcaaga aaaaacacat
     2761 ctcatcatct agctgctaga gggtttgatt ttgaaacgat cgcaagtgcg attgatgttt
     2821 tttgtactgc tacagcattt gaatcaatgt tagatcttgc tcgcgataaa ggctgaatat
     2881 cgagaggagt gacgggttgt tatcattagc ctcagagtag atctgaggcc ttttgggcgg
     2941 tgtatgcagg agatgttatg gtaaattgga ctctctacag gagagtgtgt ttgtcagcct
     3001 attaactttt cggattaccg cgcttcttag gtctattaac ttcacttttt cgattcgcct
     3061 ctctttcttc gataagctct ttcgaaaaac cttccagcag gtctaccagt ccgttcagat
     3121 cgtaatggtt tgttgttctg gcattgtctt tcccttcttt gtatcgaggt atccgcttga
     3181 tggcaccaca ctgttccaaa tgaataaaac ttctttgaac ggtttttagt gaaacgccca
     3241 gcatgttcgc cagtctcttt ttcgaaggtc ttggggggtt atctttttcc caccaatgca
     3301 ttatgaggtt tatgagaacc atcgtatcaa tacagctaag acccaacctg gactgatttt
     3361 ccagtaaggc atttgggata atcgtccatc ctgcagaaat gtttgtcttc ccccacttgc
     3421 ttacgtggtt ctttacttcg ctcgacaggt tatttttgtc ttccataaaa catgcatcct
     3481 gtggtcatct gaggctagag ggcattttat atattgaacg ttttaccgtt caccttgcgt
     3541 cattgtctta acgtattgtc ccactatatc tttttctgtg tggtcaaatc agaccgtacc
     3601 ctatggtttt taaagtccac agggtggggt ttaaatgtac cataggatat ggtctttgtt
     3661 gaccactaac caaaatgtta gcaaatgatt taaagtggtt tttttatggc aaaatgttta
     3721 ctggtcaagg tcaggaggga gacgagcgtg gcggttttta gacaatgcga aattagagaa
     3781 aaatatctcg cgatttacaa agagtcattc gatgaagtta taacttatct tgagaaggca
     3841 ggtgcttta
//
