LOCUS       NZ_CP067302             3849 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain ABW_A33 plasmid pABW_A33c, complete
            sequence.
ACCESSION   NZ_CP067302
VERSION     NZ_CP067302.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214839
            Assembly: GCF_017639125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Biggel,M.
  TITLE     Characterization of fosA carrying plasmids from multidrug resistant
            Enterobacteriaceae food and environmental isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 03:39:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,790
            CDSs (total)                      :: 4,671
            Genes (coding)                    :: 4,430
            CDSs (with protein)               :: 4,430
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 69 of 241
            Pseudo Genes (incomplete)         :: 170 of 241
            Pseudo Genes (internal stop)      :: 50 of 241
            Pseudo Genes (multiple problems)  :: 48 of 241
            Pseudo Genes (short protein)      :: 4 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067302.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ABW_A33"
                     /isolation_source="waste water"
                     /db_xref="taxon:562"
                     /plasmid="pABW_A33c"
                     /geo_loc_name="Switzerland: Basel"
                     /collection_date="2015"
                     /collected_by="ILS, University of Zurich"
     gene            join(3518..3849,1..235)
                     /locus_tag="JJB24_RS23560"
                     /old_locus_tag="JJB24_23555"
     CDS             join(3518..3849,1..235)
                     /locus_tag="JJB24_RS23560"
                     /old_locus_tag="JJB24_23555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061583929.1"
                     /translation="MAVFRQCEIREKYLAIYKESFDEVITYLEKAGALEPGVLRLTTID
                     DNAIRAWKSQWKGRSRKHAHGAWDWQNLVSKRARSCKRFDVAVWGEDVLCGLSVGKLTR
                     GKKTVRMDYLEACPTAHPLEKRITMIVVAVALSVAKKVGAQHVAIFNPIKDKEEKVLKH
                     YQSYGFTQRMLYGRFLKNVLYKEVV"
                     /name="hypothetical protein"
     gene            complement(239..409)
                     /locus_tag="JJB24_RS23565"
                     /old_locus_tag="JJB24_23560"
     CDS             complement(239..409)
                     /locus_tag="JJB24_RS23565"
                     /old_locus_tag="JJB24_23560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152924371.1"
                     /translation="MSYVSERKSELVKAQQRKMDPSSPDSAPINQGLMDFILLAMKVDD
                     GFVQRGRIHGA"
                     /name="hypothetical protein"
     gene            complement(682..826)
                     /locus_tag="JJB24_RS23935"
                     /pseudo=""
     CDS             complement(682..826)
                     /locus_tag="JJB24_RS23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1370..1570
                     /locus_tag="JJB24_RS23570"
                     /old_locus_tag="JJB24_23565"
     CDS             1370..1570
                     /locus_tag="JJB24_RS23570"
                     /old_locus_tag="JJB24_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_061585996.1"
                     /translation="MTDDRTLQLRLTGFRKAEASLRLEGMDPSGTPLYESVKTRILSGD
                     ITFDEGRTEIRAHYQKRANSN"
                     /name="antitoxin VbhA family protein"
     gene            2320..2646
                     /locus_tag="JJB24_RS23575"
                     /old_locus_tag="JJB24_23570"
     CDS             2320..2646
                     /locus_tag="JJB24_RS23575"
                     /old_locus_tag="JJB24_23570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032431019.1"
                     /translation="MFGFFKKKKLYEEICKDAGMALSDGLLAQGLARNKIEAMGAGAVF
                     SQSLREAVSQGYKSSDAIAEARKNTSHHLAARGFDFETIASAIDVFCTATAFESMLDLA
                     RDKG"
                     /name="hypothetical protein"
     gene            complement(2772..3236)
                     /locus_tag="JJB24_RS23580"
                     /old_locus_tag="JJB24_23575"
     CDS             complement(2772..3236)
                     /locus_tag="JJB24_RS23580"
                     /old_locus_tag="JJB24_23575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061583928.1"
                     /translation="MEDKNNLSSEVKNHVSKWGKTNISAGWTIIPNALLENQSRLGLSC
                     IDTMVLINLIMHWWEKDNPPRPSKKRLANMLGVSLKTVQRSFIHLEQCGAIKRIPRYKE
                     GKDNARTTNHYDLNGLVDLLEGFSKELIEEREANRKSEVNRPKKRGNPKS"
                     /name="hypothetical protein"
     Typing          complement(719..1319)
                     /name="rep_cluster_2324"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.05"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 ggattatcta gaggcttgtc caacagctca tccgttggag aaacgaatca caatgattgt
       61 ggttgctgtg gctctgtcag tagctaaaaa agtaggggca cagcacgtag ccatattcaa
      121 cccgataaaa gacaaagaag aaaaagtgtt aaaacactac cagtcttacg ggtttacaca
      181 aagaatgttg tatggacgct ttttgaaaaa cgtgctgtac aaagaagttg tttgatagct
      241 atgcgccgtg aatacgacca cgttgaacaa agccgtcatc tactttcata gcaagcagga
      301 tgaagtccat aagaccctga ttgattggag ctgaatcagg agaggatgga tccattttgc
      361 gttgctgtgc ctttaccagc tcacttttgc gctcagatac ataactcatc ttgtgactct
      421 ctgtcttgta tggggatgta gacactataa caagtggata aaaaaataac gagatctcta
      481 tattttcaaa aagagatgac tgttgttgac tcgcctcttc tgcaaagcgt actcttttct
      541 ttcaaaagaa aaaaaccgcc tttgcggggc ggtttttttc cagttcattg agtttgcggc
      601 tctttgaacc taggtaacag tcttgtgcga gacgtgtcac caaatctgtc cttccagtgt
      661 agctgtagtc aggctttaac ttcaagaact ctcgatacac ctctcgcact cactgctacc
      721 aatggctacc gccagtggcg ctttgtcgtg tctttcaggg ttggattcaa gagtacagtt
      781 acctgataaa gacgtggtag tcgggctgaa cggggagttc gtgcatacag cccagcttgg
      841 agcgaaccac ctacaaggaa cccagttgca cgggtaggaa tgagaaaacg ccaccttacg
      901 gcggactgca acgggtaaac ctcagggtgg gaacaggaga gcgcacgagg gagcgaccca
      961 tcggaaacgg tggggatctt taagtcctgt caggtttcgc cacttctgat ttgagcgccg
     1021 gaaatcacct gatttctgtg aagcttgtca ggagggcgaa gcctatggaa aaccggcaat
     1081 ccccagaacc tccttatggc cttctgcctg agcaggtaac gctcgccgca gtgtcatgac
     1141 cgaacgaagt gagcgaatga ccgaggaagc gtaatgtctg taatgctgaa cagggatcgt
     1201 gtcgctcggt acacacgttt cctccctgat tacacacccg gctgcaacag tttcctgtcg
     1261 ttttccactt ttccctgcac agctatacaa cggcaacatc cgggcaggac atgtaaagtt
     1321 agctaacttt acgcctgcct tattcgcatg actgctcaaa ggaaaaacca tgactgatga
     1381 tcggacactg caactgcgcc tgacagggtt ccgtaaagcg gaggcctctc tcaggcttga
     1441 agggatggat ccatcaggaa ccccgcttta tgaatcggta aagacaagga ttctttccgg
     1501 tgacatcacc ttcgatgaag gccgcactga gatacgggct cattaccaga agcgggcgaa
     1561 cagtaactga cgcggacagg aaatgttttg tggccaccgc cgctgcgccg gcgccacaaa
     1621 atggcgggac aagatgtgtt tttggagtac cgccggcact ccagaatgtt tcatcaggcc
     1681 ccgcccgata ccctgaattt cgtacgtacg aaatttcgct gctgttcaga tcgagagcag
     1741 gattcgcaaa ttgctggcgt ttgtacccac tgtaaatctg ccgcgcctgg cggcttggcc
     1801 aacgcttcac gaacgcacag taaactgcgc actcgttcac cgttaccaga aaggccattg
     1861 cgggaataat tctttcagtc gttctacgag atcaagcagc tcattcatga ccttcagcat
     1921 gacgcttatc agtttcaaaa atttaggcat ataaaaattt cctgtacgga gttgggtctg
     1981 gatcaggaaa ttagggactt tgtaattttc agcggtagaa aaatcagttt ttctaaaaaa
     2041 attttttagg cggcgaaaag tgaagtaatg agcactgctt cgtgctgaaa atgaggctca
     2101 ggcaggtgtt ttgcagcgaa ggcgcgcgtc attcactctc gtgcatagcc atgcagcggc
     2161 ctaaaaatca tcaggggcgt ttttcaggag tatggacagg gaaaggtgcc ggaaacggcc
     2221 cggacgcccc aggatcgcgc tgaggggctc tcagggggtg cgggcaaggg atgttatggt
     2281 aaattgctca tcacagtcgc cagttaagca gaggttagaa tgtttgggtt ttttaagaaa
     2341 aaaaagctct atgaagagat ctgtaaagat gcagggatgg ctttatctga tgggttattg
     2401 gcccaaggct tagcgagaaa caaaatagaa gcaatggggg ctggtgctgt ttttagtcag
     2461 tctttgaggg aggctgtttc tcaaggttac aagtcttctg atgcgattgc tgaagcaaga
     2521 aaaaacacat ctcatcatct agctgctaga gggtttgatt ttgaaacgat cgcaagtgcg
     2581 attgatgttt tttgtactgc tacagcattt gaatcaatgt tagatcttgc tcgcgataaa
     2641 ggctgaatat cgagaggagt gacgggttgt tatcattagc ctcagagtag atctgaggcc
     2701 ttttgggcgg tgtatgcagg agatgttatg gtaaattgga ctctctacag gagagtgtgt
     2761 ttgtcagcct attaactttt cggattaccg cgcttcttag gtctattaac ttcacttttt
     2821 cgattcgcct ctctttcttc gataagctct ttcgaaaaac cttccagcag gtctaccagt
     2881 ccgttcagat cgtaatggtt tgttgttctg gcattgtctt tcccttcttt gtatcgaggt
     2941 atccgcttga tggcaccaca ctgttccaaa tgaataaaac ttctttgaac ggtttttagt
     3001 gaaacgccca gcatgttcgc cagtctcttt ttcgaaggtc ttggggggtt atctttttcc
     3061 caccaatgca ttatgaggtt tatgagaacc atcgtatcaa tacagctaag acccaacctg
     3121 gactgatttt ccagtaaggc atttgggata atcgtccatc ctgcagaaat gtttgtcttc
     3181 ccccacttgc ttacgtggtt ctttacttcg ctcgacaggt tatttttgtc ttccataaaa
     3241 catgcatcct gtggtcatct gaggctagag ggcattttat atattgaacg ttttaccgtt
     3301 caccttgcgt cattgtctta acgtattgtc ccactatatc tttttctgtg tggtcaaatc
     3361 agaccgtacc ctatggtttt taaagtccac agggtggggt ttaaatgtac cataggatat
     3421 ggtctttgtt gaccactaac caaaatgtta gcaaatgatt taaagtggtt tttttatggc
     3481 aaaatgttta ctggtcaagg tcaggaggga gacgagcgtg gcggttttta gacaatgcga
     3541 aattagagaa aaatatctcg cgatttacaa agagtcattc gatgaagtta taacttatct
     3601 tgagaaggca ggtgctttag agcccggcgt tttaaggctc actacaatcg atgacaatgc
     3661 tattcgtgca tggaagagcc aatggaaagg caggtctcgt aagcacgctc atggtgcttg
     3721 ggattggcag aaccttgtat caaagagagc tcgttcttgt aaaagatttg atgtggctgt
     3781 ttggggagag gatgtgcttt gtgggctttc agttggaaag ctaacccgtg gtaaaaagac
     3841 agttagaat
//
