LOCUS       NZ_CP067273             2348 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain ABW_S38 plasmid pABW_S38h, complete
            sequence.
ACCESSION   NZ_CP067273
VERSION     NZ_CP067273.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214841
            Assembly: GCF_017639105.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Biggel,M.
  TITLE     Characterization of fosA carrying plasmids from multidrug resistant
            Enterobacteriaceae food and environmental isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 04:00:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,122
            CDSs (total)                      :: 5,007
            Genes (coding)                    :: 4,821
            CDSs (with protein)               :: 4,821
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 46 of 186
            Pseudo Genes (incomplete)         :: 129 of 186
            Pseudo Genes (internal stop)      :: 37 of 186
            Pseudo Genes (multiple problems)  :: 27 of 186
            Pseudo Genes (short protein)      :: 5 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067273.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ABW_S38"
                     /isolation_source="waste water"
                     /db_xref="taxon:562"
                     /plasmid="pABW_S38h"
                     /geo_loc_name="Switzerland: Basel"
                     /collection_date="2015"
                     /collected_by="ILS, University of Zurich"
     gene            complement(join(1668..2348,1..48))
                     /locus_tag="JJB13_RS25320"
                     /old_locus_tag="JJB13_25315"
     CDS             complement(join(1668..2348,1..48))
                     /locus_tag="JJB13_RS25320"
                     /old_locus_tag="JJB13_25315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_012564910.1"
                     /translation="MGNSKRNIKKLNDNFREDILDYAIAHNLKCANALAILYATGCRPD
                     ELQTGVTVNYDSKKNEIEFRIIGSKLNRRMRRGIGVRKIKVKINNENARFFKNIVDKFI
                     ENPMSYDHKIKIESAKAFSGYITKISKKLWPRKTYHASAYSFRHAKATELKNSDYDKIE
                     IAQIMGHASVRSQQSYGRKSKKSKGGFDDIADVETNVKPRGGDRLLRFKIANKNKAAAK
                     IADTSTPSSPPPAPVRRFKM"
                     /name="site-specific integrase"
     gene            complement(416..1162)
                     /locus_tag="JJB13_RS25325"
                     /old_locus_tag="JJB13_25320"
     CDS             complement(416..1162)
                     /locus_tag="JJB13_RS25325"
                     /old_locus_tag="JJB13_25320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_071681991.1"
                     /translation="MDIGNILNESLNVEYSKLDLFLEKYDLTPEQKVEVYEFHAKASKK
                     NKTLVISETKENRFKSISEGVEYVHLFPKNLKILIKKYGLNTNELLVLTEIMESMLSHG
                     NLLINFSQKTLCELTEINKSTMSKTFKSLKQKQCLIEKNGHIYLNSVIFMKGLPHKLFI
                     QFRDHFLNSISYKLDDEEEFEKVFDDNFIKAYEKNLKEIKKKKQQIKEKKISKALDNFE
                     KEISEEWKEKFKDEEENFEFGFESEM"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1424..1606)
                     /name="Col(IRGK)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.7e-91"
ORIGIN
        1 tctaaaatta tcatttagtt tttttatatt tcttttcgaa ttacccatat tgatttttcc
       61 tcattgcaaa ttaaaagaac cggaaatttc aaaaattcgc tttgctcaaa tttgaaatac
      121 cgtaaaaccc aaaccctccc cccttatgtt caatttttcg ctatcgctca aatcgaacaa
      181 cgacccccgg ttgggggtgg caattttatg gccttcgctt tgctctatcc ctaaaatgcc
      241 ttgttgttcc acccaaaaag acgggttgaa acaactactt atagtactgt tataaccggg
      301 tggaaaaaac tgtcaatatt tgaaatgtgg agaattaatg gataaaaaaa atcccttagt
      361 ggaatagaat ttattctatt tcagtaaggg attcgcctgc ccggcgaggg cgattttaca
      421 tttcagattc aaaaccaaat tcgaaatttt cctcttcgtc tttaaatttt tctttccatt
      481 cttcagaaat ttctttttca aaattatcta atgcttttga tattttcttt tcttttatct
      541 gttgtttttt ctttttaatt tctttgagat ttttttcata tgctttaata aaattatcgt
      601 cgaagacttt ttcaaactct tcttcatcat ctaatttata agaaattgaa tttaaaaaat
      661 gatctctaaa ttgaataaac aatttatgag gcaaaccttt cataaatatt acagaattta
      721 aataaatatg cccgtttttc tcaattaaac attgcttttg tttaaggctt ttaaatgttt
      781 tcgacatagt agatttatta atttctgtta attcgcaaag tgttttttgc gaaaaattaa
      841 ttaataaatt tccgtgtgaa agcattgatt ccattatttc cgttaaaact aataattcat
      901 ttgtgtttaa accatatttt ttaattaaaa tttttaagtt tttcggaaac aaatgcacat
      961 attcaacacc ttcggaaata gatttaaatc tattttcttt tgtttccgaa ataaccaaag
     1021 tcttattttt cttagacgct ttcgcgtgaa attcataaac ttccactttt tgctcaggtg
     1081 ttaaatcata tttttctaaa aacaaatcta attttgaata ttcaacattc aaactctcat
     1141 ttaatatatt tccaatatcc atagaaacct cctcttttta tttatttcta tatatatgtt
     1201 ttaacattta taaagaacta gtcaatagat tgagttgcaa aaggtagaaa aagtttcagt
     1261 tttgaaactg tagtttctac ttggaaactg gctagagttg tcggatttga caacctcagt
     1321 tgacgaattt gacaactaga gttgacgaat ttgacaacta cggttgaagt ttttgacaac
     1381 tgcctttttt taaaatccag taatatcaat ggttagagag tagtttttta actcctaaga
     1441 acatctaaga attttaagaa cactaagggg gtgaaatttt caacttttga gatggctttt
     1501 aagatcattg gttttcaagt aacagatcaa tcccccacgg gggaaaacca tccagttacc
     1561 cgttccgggc ttctgcttcg ctatggagga ttttgctgta aaaccgtcct gaccggacgg
     1621 gccaaccctt cacagtccag gagggaacct cctgaactgc tcacggttca cattttgaag
     1681 cgacgaacgg gagccggtgg aggactgctg ggggtggaag tatcggcaat ttttgccgct
     1741 gctttgtttt tatttgcgat cttaaatctc aataatctat caccgccacg gggtttaaca
     1801 ttggtttcga catctgcgat gtcatcaaat ccacctttgc tttttttgct ctttcttccg
     1861 taactctgct gagatctaac tgaggcatgg cccataatct gagctatttc gattttatca
     1921 taatcggaat tttttaattc cgttgctttt gcatgtctaa aagaatatgc agaagcatga
     1981 taggttttcc tgggccatag ctttttcgat atttttgtta tgtacccgga aaatgctttg
     2041 gcactttcaa ttttgatttt gtgatcatat gacattgggt tttcaataaa tttatcaaca
     2101 atgtttttaa aaaacctggc attttcatta ttgattttta cttttatttt tctaacccct
     2161 atgcctcttc tcattcttct atttagtttt gatccaatta ttctaaattc aatttcattt
     2221 tttttactgt catagtttac agtaactccg gtttggagtt cgtccggacg gcaacccgtt
     2281 gcgtataaaa tagcaagtgc gttagcacat tttagattgt gcgcgatcgc ataatctaaa
     2341 atatcctc
//
