LOCUS       NZ_CP067272             4183 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain ABW_S38 plasmid pABW_S38g, complete
            sequence.
ACCESSION   NZ_CP067272
VERSION     NZ_CP067272.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214841
            Assembly: GCF_017639105.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Biggel,M.
  TITLE     Characterization of fosA carrying plasmids from multidrug resistant
            Enterobacteriaceae food and environmental isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 04:00:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,122
            CDSs (total)                      :: 5,007
            Genes (coding)                    :: 4,821
            CDSs (with protein)               :: 4,821
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 46 of 186
            Pseudo Genes (incomplete)         :: 129 of 186
            Pseudo Genes (internal stop)      :: 37 of 186
            Pseudo Genes (multiple problems)  :: 27 of 186
            Pseudo Genes (short protein)      :: 5 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067272.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ABW_S38"
                     /isolation_source="waste water"
                     /db_xref="taxon:562"
                     /plasmid="pABW_S38g"
                     /geo_loc_name="Switzerland: Basel"
                     /collection_date="2015"
                     /collected_by="ILS, University of Zurich"
     gene            complement(297..1490)
                     /gene="mobQ"
                     /locus_tag="JJB13_RS25295"
                     /old_locus_tag="JJB13_25290"
     CDS             complement(297..1490)
                     /gene="mobQ"
                     /locus_tag="JJB13_RS25295"
                     /old_locus_tag="JJB13_25290"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_000190114.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYVDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     gene            1732..1896
                     /locus_tag="JJB13_RS25705"
     CDS             1732..1896
                     /locus_tag="JJB13_RS25705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162865999.1"
                     /translation="MEKETLGPDIQVNWCDHCEKNTRHIFRFTEEYSKEEGPITEVECT
                     KCGNTFTIL"
                     /name="hypothetical protein"
     gene            1913..2302
                     /locus_tag="JJB13_RS25300"
                     /old_locus_tag="JJB13_25295"
     CDS             1913..2302
                     /locus_tag="JJB13_RS25300"
                     /old_locus_tag="JJB13_25295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223168448.1"
                     /translation="MQYRGLEFEEQEFNDCLNILHKEVVSFLNKNNMALDFFQYPVEDI
                     CRIIFRDKIDLRTAVILDFGITTYDLSPLGDHDPFVWRLLEWPLYDYENFQFVIVQKLA
                     SYELDKLNEIIQVEDNKDNKDKRRL"
                     /name="hypothetical protein"
     gene            2304..2573
                     /locus_tag="JJB13_RS25305"
                     /old_locus_tag="JJB13_25300"
     CDS             2304..2573
                     /locus_tag="JJB13_RS25305"
                     /old_locus_tag="JJB13_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_000047246.1"
                     /translation="MSKSTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LDKKPEEINKALILGLLSKINELDEEEKRDFTRIGMDILQQWKS"
                     /name="conjugal transfer protein TraD"
     gene            complement(2819..3088)
                     /locus_tag="JJB13_RS25310"
                     /old_locus_tag="JJB13_25305"
     CDS             complement(2819..3088)
                     /locus_tag="JJB13_RS25310"
                     /old_locus_tag="JJB13_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="WP_021197950.1"
                     /translation="MRRSTIRKGLTQKEMAERFNISVKTVKKYTSEPREQYEQTAADRR
                     RQAYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPKNSL"
                     /name="response regulator transcription factor"
     gene            complement(3085..3942)
                     /locus_tag="JJB13_RS25315"
                     /old_locus_tag="JJB13_25310"
     CDS             complement(3085..3942)
                     /locus_tag="JJB13_RS25315"
                     /old_locus_tag="JJB13_25310"
                     /inference="COORDINATES: protein motif:HMM:NF015076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000586611.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEKVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQEKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPLNPLQYKELKATARSIARWTWKNFDSHTFSLIQSSRGAKNKNKPKKSNVKEMLGFI
                     GGEK"
                     /name="replication initiation protein"
     Typing          complement(481..1490)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.703"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 tagaaaaaca ataaaaaaag aaaaaagagt attgttcgaa gaaaaattgg cgattcgatt
       61 ttttttggtg aatatatgaa aaaaaagaaa gtgcagaaaa aacggaaaag aaataagcat
      121 agtaaatttt ttaaaatgct tatgaagtca gttaatgaag ctgtggcaat acataaaggg
      181 gaaatagaac cggcgaggat cacaactcta aaaatgtagt atattatagt atagtattaa
      241 ataatattaa gttgtattaa gtgatactac tatataggaa tatataccac tatatactac
      301 attccaaatt tcttcttcct cttaggttgc tgatcttcga attccggcat tggttccggc
      361 tcaggctctt caatttttgg ttgtggttgg ttcataggag acattccaga gcctgaaccc
      421 atttttccgc ctccattatt tccaccggtt ccggttcctg tgcctgtttt aggttttgtt
      481 tctaactctg tctttaatat atgttcgcca aattcattta ttacataact actaacgaaa
      541 ttttgaggat cttctttttc ttctttttta tttttattta cattaggttt tgatttaaat
      601 atatgctggg tctttttgta taatgccttt aaattatcaa tgattgacct tgcaagaggc
      661 tttttctcta ttttcatagt ttcctctggc tgaggtggtt tggtttgctg taagtatgtt
      721 gcattagcta tttttaataa taatgctttt tctttttgct ccatagctct taatttttga
      781 ccttttcgcc attggtgcct gtgtatcctg gtcattggcg ttctgtccag ttcaatagtt
      841 tttgaaatcc acttggcttt ctctttcgga gttttagcaa aaacagcctt tctttctgcc
      901 tcttcttttt gatcttttaa tgaacgggaa tcaatacggg acttatgacc atattttttt
      961 aatctgtcat ttgctattat ttcccagtta actcgccagg cttcaatatt ttctctatta
     1021 ttccattctc tttgtttgtt cccaaatcca tttgggctaa cagttcttaa agttagcatt
     1081 acatgagcat gagggttatg actatcaaga tcatgaaatg ctatgtctgc aatcattcca
     1141 taatccacga aattttcttg gcagtaatcg aggacgatct gcttcttctg gccgtcgtcc
     1201 agctccttgg ggatggctat gtccacatag cgacataatt cggcatcctt tcgcttctcg
     1261 ccagcctcta cggcattcca gagggtgcct gagtccttca ccagttcggc aggtgtgccg
     1321 gcaggggaga ggatagcaga gccgaaaaat ccctcttttt ttgaataatc ccaggttgcc
     1381 ccggttcgat tatcttttgt cttttctcgg ttttgatatg cagataaata aaccgaagat
     1441 ttaccttctg atcttcgtaa ttttttaaag ctgatatgaa atgtcgccat agtctacccc
     1501 ttactttttt tgtcttttga attttggttt ttagccccgc agggcccata cgtcgcttgc
     1561 gacgtgtaag tgggcattac taatattatt ttttcggctt cacctcaaaa taataagtaa
     1621 tgaaccccag gcttcgccca atattaaata ttatttattt tattctaaaa tatatttgac
     1681 taaagtaaat aatggaggta ataaataaca aaataaaata atggaggtta tatggaaaaa
     1741 gaaacgctcg gccctgatat acaagtcaat tggtgtgatc attgtgaaaa aaacactcgc
     1801 catatattta gatttactga ggagtattca aaagaagagg gaccaattac agaggttgaa
     1861 tgtactaaat gtggtaatac attcactatc ttatagtgtg gaggttttta atatgcaata
     1921 tagaggttta gagtttgaag aacaagagtt taacgactgt ttaaacattt tacataaaga
     1981 ggtagtgtct tttttaaata aaaataatat ggctctggat tttttccagt atccagttga
     2041 ggatatatgt agaataattt ttcgagataa aatagatctg agaactgctg ttattttaga
     2101 ttttggaatt acaacatatg atttaagccc tttgggagat cacgatcctt ttgtttggcg
     2161 gttgcttgaa tggccattat atgattatga gaatttccag tttgtgattg ttcaaaaact
     2221 ggcttcttat gaattagata aattaaatga aataatccaa gtagaagata acaaagataa
     2281 taaagataaa aggaggttgt aatatgagca aatcaacagc acagcagatc gcagatctac
     2341 aaaataaatt acaagcacta agaacaaaaa aagcaaggca ggacagacgg gaagaaacaa
     2401 gacaaaaaat aatcctgggt gcagaagtgg ctaaggtgtt agataaaaaa ccagaggaaa
     2461 ttaacaaggc tttaattctt ggcttgttaa gtaaaatcaa tgagttagac gaagaagaga
     2521 aaagagattt tacacgaatc ggaatggata tattgcaaca atggaaatcg taaaccgtga
     2581 acagtgaaga accagccgct ttttgggctg gttttttgct gtgaagggtt gccagccaac
     2641 ggctggaggt tttaaggttg ccagagggcc tccggtggct gggtgggttt gttgatttgt
     2701 tggtaccaga gtgtccctca cctatccaga cacaagcccg gatacgggga aagggggaaa
     2761 aatcccccca ggggtggagg gtttggttag gttaggggga aaaactgctt agaagtcgtt
     2821 acaggctgtt tttaggcata tctaaggcaa cataacggcg atacagggca acggctccgt
     2881 gatacgaaca gcccattttg tcggctacct gttgccattt cagcccctgg cttcgtagtt
     2941 cgtaagcctg acgacgacga tcagccgctg tttgttcgta ttgttcacgc ggttcggatg
     3001 tatatttttt taccgtttta acagatatat taaatctttc agccatttct ttttgagtta
     3061 aaccttttct tattgtgctt cttctcattt ttcacctcct ataaaaccaa gcatttcttt
     3121 aacattactc ttcttgggtt tgtttttatt tttcgcacct cttgaagatt gtattagtga
     3181 aaatgtatgg ctatcaaaat ttttccaggt ccaacgggct atgcttcttg ctgttgcctt
     3241 taactctttg tattgtaacg gatttagtgg attgcaaaaa gtatttaaac cctgtgcttt
     3301 taataatagt tcgttttcga atttctcgaa agtagatatt tttttgaaat ttaaaaccgt
     3361 tctgtaagca tattgtcgta gatcttcaaa caaattaaca ttgcgaccta aaccataagc
     3421 ctcgcttttt ttctcttgta tttgaggttt ttctaagtcc acaaaatcag ctaaataatc
     3481 caggtcgtaa aggtcagcac ctgaccagaa cggcgaccag tgatcattga gtggattttt
     3541 tgtaatcaat ccagcaaacc ccagatctgc tttgagtttt ttcgccatag ccatttcaac
     3601 ggcagcggca tacttcaacg gcttcaacct tgcttgttgt gtcttacaaa caccggcttt
     3661 aagcatgtac ccgtaatggc aacgacctgt ctttggtgtt tttgtaataa agtgaggtat
     3721 gggtaagccc accttctcaa atgtatagaa tgagtttttc ttatctaaat caaagagtaa
     3781 gcaagtttgg attgctggct gatttgcctg taaatacagc atttctaagg cttttgcctt
     3841 gttccttatg cttaatccca tatcgaggtt atttgtacaa taaggcttgt taggcatttg
     3901 atttttaaaa aaatcgatgt tatgttcttc ttgtattatc attttttcag tcctttttat
     3961 ctggccctac tctctgtttt ctgtcgccaa aacaaaatcg cagagggtgg ggtttttatt
     4021 tttataggtt tttcaatcct gtaccagttc taaccgatct taaaaatttg tcaagtgggg
     4081 agcttaacca tatcagataa cagcccaacg aagttggggc agttctttac atacggcagc
     4141 aaagcattac tatcagatct atggtgcgaa gcattaccga aga
//
