LOCUS       NZ_CP067260             3005 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain ESBL_DR09 plasmid pESBL_DR09e, complete
            sequence.
ACCESSION   NZ_CP067260
VERSION     NZ_CP067260.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214843
            Assembly: GCF_017639065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Biggel,M.
  TITLE     Characterization of fosA carrying plasmids from multidrug resistant
            Enterobacteriaceae food and environmental isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:55:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,450
            CDSs (total)                      :: 5,328
            Genes (coding)                    :: 5,207
            CDSs (with protein)               :: 5,207
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 54 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 16 of 121
            Pseudo Genes (multiple problems)  :: 17 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067260.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ESBL_DR09"
                     /isolation_source="imported food: chilli pepper"
                     /db_xref="taxon:573"
                     /plasmid="pESBL_DR09e"
                     /geo_loc_name="Switzerland: Zurich"
                     /collection_date="2014"
                     /collected_by="ILS, University of Zurich"
     gene            complement(86..247)
                     /locus_tag="JJB15_RS27455"
     CDS             complement(86..247)
                     /locus_tag="JJB15_RS27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071784682.1"
                     /translation="MHEPPVQPDHCALSGNYRLESNPVRHAKAPLAAAIGNRVSRARFV
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(734..928)
                     /locus_tag="JJB15_RS27145"
                     /old_locus_tag="JJB15_27150"
     CDS             complement(734..928)
                     /locus_tag="JJB15_RS27145"
                     /old_locus_tag="JJB15_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041644.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1201..1524
                     /gene="mobA"
                     /locus_tag="JJB15_RS27150"
                     /old_locus_tag="JJB15_27155"
     CDS             1201..1524
                     /gene="mobA"
                     /locus_tag="JJB15_RS27150"
                     /old_locus_tag="JJB15_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(1833..2216)
                     /locus_tag="JJB15_RS27155"
                     /old_locus_tag="JJB15_27160"
     CDS             complement(1833..2216)
                     /locus_tag="JJB15_RS27155"
                     /old_locus_tag="JJB15_27160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_022631129.1"
                     /translation="MKFFKPKTDAIEYIRVEPSFSKTSSNINNAKSGSDTNSQPGFILR
                     SQIEEMIKNHRPKTEKEIKAESEGLIRLCSMAFIFLIVLSIFMLIVYFGIKDRELPDFA
                     NNLMALMTTLVGAIGGFIYGTKK"
                     /name="hypothetical protein"
     gene            complement(2213..2890)
                     /locus_tag="JJB15_RS27160"
                     /old_locus_tag="JJB15_27165"
     CDS             complement(2213..2890)
                     /locus_tag="JJB15_RS27160"
                     /old_locus_tag="JJB15_27165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023233475.1"
                     /translation="MENKAVTISTQLAIFIDSTIARPDLLFNSLNSEIGEVIDAMPQTL
                     PLPADAPADIPRVIGTSSFGKFNLNISLNRIDFVENYTPTEEIEKSVTEFKLKCNTLIA
                     TIFQKYGVVRIGLVGNYYIPHKNPSQLIADLYLNENKRNLDEITIRINNRDTFKNIQIN
                     NVINLSQGQVTAQNYNGNAVIVQLDYNSTDNNTPLDEGLTLALFKEKSIAYNSKHVEDV
                     SGL"
                     /name="hypothetical protein"
     Typing          966..1192
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.833"
                     /coverage="99.0"
                     /evalue="1.04e-81"
     Typing          complement(176..705)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.6"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1202..1521
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="1.96e-62"
ORIGIN
        1 ctggcagatt gtctaaccgg gtaactggct tcagcagagc gcagatacca aatactgtcc
       61 ttctagtgta gccgtagtca ggccatcact tcaagaactc tgtaagcatc tggacaaatc
      121 tcgctctgct aactcggtta ccaatggctg ctgccagtgg tgcttttgca tgtcttaccg
      181 ggttggactc aagacgatag ttaccggata aggcgcagtg gtcgggctga acggggggtt
      241 cgtgcacaca gcccagcttg gagcgaacga cctacaccgg gccgagatac ctacagcgtg
      301 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      361 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      421 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      481 gggggcggag cctatggaaa aacgcctgcg gtgctggctt cttccggtgc tttgcttttt
      541 gctcacatgt tctttccggc tttatcccct gattctgtgg ataaccgtat taccgccttt
      601 gagtgcgctg acaccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag
      661 gaagcggaag agcgccttat gtgacatttt ctccttacgc tctgttgtgc cgttcggcat
      721 cctgccctga gcgttatatc tctgtgctat tttctacttc aaagcgtgtc tgtatgctgt
      781 tctggagttc ttctgcgagt tcgtgcagct tctcacacat ggtggcctgc tcgtcagcat
      841 cgagtgcgtc cagtttttcg agcagcgtca ggctctggct ttttatgaat cccgccatgt
      901 tgagtacagt ttgctgctgc ttgttcatct ttctgttttc tccgttctgt ctgtcatctg
      961 cgtcgtgtga ttatatcgcg caccactttt ggaccgtctt accgccggta ttctgccgac
     1021 ggacatttca gtcagacaac actgtcactg ccaaaaaact gccgtgcctt tgtcggtaat
     1081 tcgagcttgc tgataggaca ggatgtgtaa ttgttatacc gcgcatacat gcacgctatt
     1141 acaattaccc tggtcagggc tttgccccga caccccatgt cagatacgga gccatgtttt
     1201 atgacaaaac gaagtggaag taatacgcgc aggcgggcta tcagtcgccc tgttcgtctg
     1261 acggcagaag aagaccagga aatcagaaaa agggctgctg aatgcggcaa gaccgtttcc
     1321 ggttttttac gggcggcagc tctcggtaag aaagttaact cactgactga tgatcgggta
     1381 ctgaaagaag ttatgagact gggggcgtta cagaaaaaac tctttatcga cggcaagcgt
     1441 gtcggggata gggaatatgc ggaggtgctg atcgctatta cggagtatca ccgtgccctg
     1501 ttatccaggc ttatggcaga ttagcttccc ggggagaaaa ctgtcgaaaa cagacggtat
     1561 gaacaccgta agcccccaaa ccgatcgcca ttcactttca tgcatagcta tgcagtgagc
     1621 tgaaagcaat cctgacgcat ttttccggtt taccccgggg aaaacatctc tttttgcggt
     1681 gtctgcgtca gaatcgcgtt cagcgcgttt tggcggtgcg cgtaatgaga tgttatggta
     1741 aatggtgtca ggtaacggtt gtaaaagccc cgaagtatgg ggctggtgtg cttgtgtgtt
     1801 atttgttttt tttatttgtg tttttgtctg tgttattttt ttgttccata tataaaacca
     1861 cctatcgctc caactaatgt tgtcattagt gccatcaggt tatttgcaaa atctggtagt
     1921 tctctatctt ttattccgaa ataaacgatt aacataaaaa ttgataaaac tattaaaaat
     1981 ataaaggcca tggagcaaag ccttataaga ccttcgcttt cagcctttat ttctttttct
     2041 gttttcggtc tgtggttttt tatcatttcc tctatttgag atcttaaaat gaatccaggc
     2101 tgtgagtttg tgtcacttcc tgatttcgca ttatttatat tgctacttgt ttttgagaat
     2161 gatggttcta cacgaatgta ttcaatcgca tctgtttttg gtttgaaaaa tttcataatc
     2221 cactcacatc ctcaacatgc ttggagttat aagcaatgga tttttcttta aatagggcta
     2281 aggttaaccc ttcgtcaagt ggtgtgttat tatcggttga gttgtagtca agttgaacta
     2341 tcaccgcatt tccattgtag ttctgtgcgg ttacttgtcc ttggcttaaa ttgatgacat
     2401 tgtttatttg tatatttttg aatgtatcgc ggttatttat tcttattgtt atttcatcta
     2461 aatttctttt gttttcgttt aaatacagat cagctattag ttgagatgga tttttatgtg
     2521 ggatgtaata gttaccaact aggccgattc ttacgactcc atatttttgg aatattgttg
     2581 ctattagcgt attgcacttt aatttgaatt ccgttactga tttttcgatc tcctcagtag
     2641 gggtgtagtt ttctacaaaa tctattctgt ttagtgatat gtttaagttg aacttgccaa
     2701 aggacgatgt gccaataacg cgagggatat ctgcaggtgc atctgcaggc aggggaagcg
     2761 tctgtggcat tgcgtctata acttcgccaa tttctgagtt taggctatta aagagaagat
     2821 ctggtctagc tatggtgctg tcaataaata ttgctagctg tgttgatatt gtgacggcct
     2881 tgttttccat tttttctttc ctattttgca ttggtattaa ttgaagtatt gcgaaattaa
     2941 cgtgttgtga gtatcttaaa cgaaaaaacc accctggcag gtggtttttc gaaggttagc
     3001 taatc
//
