LOCUS       NZ_CP067259             3357 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain ESBL_DR09 plasmid pESBL_DR09d, complete
            sequence.
ACCESSION   NZ_CP067259
VERSION     NZ_CP067259.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17214843
            Assembly: GCF_017639065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Biggel,M.
  TITLE     Characterization of fosA carrying plasmids from multidrug resistant
            Enterobacteriaceae food and environmental isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore MinIon
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:55:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,450
            CDSs (total)                      :: 5,328
            Genes (coding)                    :: 5,207
            CDSs (with protein)               :: 5,207
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 54 of 121
            Pseudo Genes (incomplete)         :: 69 of 121
            Pseudo Genes (internal stop)      :: 16 of 121
            Pseudo Genes (multiple problems)  :: 17 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP067259.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ESBL_DR09"
                     /isolation_source="imported food: chilli pepper"
                     /db_xref="taxon:573"
                     /plasmid="pESBL_DR09d"
                     /geo_loc_name="Switzerland: Zurich"
                     /collection_date="2014"
                     /collected_by="ILS, University of Zurich"
     gene            join(3228..3357,1..104)
                     /locus_tag="JJB15_RS27115"
                     /old_locus_tag="JJB15_27120"
     CDS             join(3228..3357,1..104)
                     /locus_tag="JJB15_RS27115"
                     /old_locus_tag="JJB15_27120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_049595772.1"
                     /translation="MTELETHLLNALKRMEQQFSEQLKASEKAQAALQSMFERTAEDNA
                     TLLKQVSFLDNRVTVLSEQLKQFGNLYRQNRR"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            complement(333..767)
                     /locus_tag="JJB15_RS27120"
                     /old_locus_tag="JJB15_27125"
     CDS             complement(333..767)
                     /locus_tag="JJB15_RS27120"
                     /old_locus_tag="JJB15_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193480.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_149053586.1"
                     /translation="MSKRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVGNHKKALTNGLSVIQGGRS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1199..1504)
                     /locus_tag="JJB15_RS27125"
                     /old_locus_tag="JJB15_27130"
     CDS             complement(1199..1504)
                     /locus_tag="JJB15_RS27125"
                     /old_locus_tag="JJB15_27130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_208770152.1"
                     /translation="MDIQQNSQIEANKSKILEAAYAGKGEIVTLDYEDDFGISRKEPVG
                     QVRIALAELVKNGLVIEKGCPDDGINVLITALGISTYEMKLFDNLPPDHPSRGKFF"
                     /name="hypothetical protein"
     gene            1686..1985
                     /locus_tag="JJB15_RS27130"
                     /old_locus_tag="JJB15_27135"
     CDS             1686..1985
                     /locus_tag="JJB15_RS27130"
                     /old_locus_tag="JJB15_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_049595769.1"
                     /translation="MEKRDNMLRVRFSDAEFEALKQLAEDAGCTMSELVRDHLGRVSVR
                     NKDVDRERIAMLNRINANLNMIARWVNTHKSAASSVEVVAHLMDIERHIRELSQ"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(2072..2482)
                     /locus_tag="JJB15_RS27340"
     CDS             complement(2072..2482)
                     /locus_tag="JJB15_RS27340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228294179.1"
                     /translation="MIRLSDLTCAPWVIRISQAIPGVDFTAQVIESLFCGISGRADVKA
                     FPGAKLNAGRHKMQLVMPGMGMPDPEDVVLIGFKPGERDLFKAIHERLLHFWRDLLLRS
                     ERQHARSVFVPEIERVDKVAGSLRGSPQHFRR"
                     /name="hypothetical protein"
     gene            2718..3224
                     /locus_tag="JJB15_RS27140"
                     /old_locus_tag="JJB15_27145"
     CDS             2718..3224
                     /locus_tag="JJB15_RS27140"
                     /old_locus_tag="JJB15_27145"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_149053585.1"
                     /translation="MSVSLTLQERLKQQHLAESETIQSLTKQQLSSLRSELNGIFQQEL
                     NTMLEDMRRQLHDTTEDMRQSRQSMLLTVVKNRVIFPALSAVTILAVIFLSGWGLSTFQ
                     ESQIMANWQQINSQKATLATLESKTAGLNIVQDKNGTFVVLPKGAKVDDSGWTVGKQQA
                     LRIVR"
                     /name="mobilization protein"
     Typing          1983..3355
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.197"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 acgccacgct gttgaagcaa gtcagcttct tagacaatcg ggtgacagta ttgtcggagc
       61 agctgaagca gttcgggaac ctctacaggc agaacaggcg ctgagaaggc agctggaatt
      121 tcaggaagcg ttgcagcaga aacagcgtga acctgagcct ccggcgccga agccgaacag
      181 gtattacgga ccgagcatgt agagttagcg ggcaaaaaaa accccgcact aggcggggaa
      241 aattcgttac agaggaatat ccctgtaacc tattggacaa tataaatata ttttggttca
      301 ccgcatcgat agttacgttt catggtaact attcagctac gcccgccctg aataacgctc
      361 aacccgttag taagggcttt tttgtggttc cccacagtac ctttccagcc aaactgaggg
      421 ttcagacggt aactgtaact gcgtcctact tttgggcctt gaagaatcac ggataggtca
      481 ataagcgcct taaccgaacg gcttacattg gttttctgca tacctaaagc gtcggcaata
      541 tcagcttggg caatttggat gtaattttca taatcgagat cagctaacag catcatcaag
      601 actttgaatt gctcccctgt gagggtttta gcaaggatct ttaaagcgtc ttggttcata
      661 gtgaaatgcc tctcaaaagc tgacttctgt ttgggtcgga taactgcgac aaaactacct
      721 acctcttccc ctgtagcagt gtctatctga gtaattcgtt tactcattta agaggctctc
      781 ccaaaaaaat agttatcaat aatgataaca acgttatcaa taatgataac tatcgtcaag
      841 cgagagccgc gccccgcaag ggctgagaga aggccctctt ctgtatctta tagcgagaag
      901 cccatatggc cgggtagcga accctatttt catcgactcg tgcgtaagca tttctatgcc
      961 cgttcaggtg atattccgct tcctcgttca ggctgttcgt tcctcgctat gctgcggtac
     1021 gcctcagcct gacctgcggc gagcggcaca tgttctttca aaagcggctt tgttagctga
     1081 aattcgtcaa gggcgagcgg agcgagtgca tttcaccctt gacgactcac gggataaaac
     1141 aagctgagta gaggtggatg gttcacgcag tgaggccatc cacctgtaag acgggttgtt
     1201 aaaagaattt accccgagat ggatggtcag gaggaagatt gtcgaagagt ttcatctcat
     1261 atgtactgat accgagtgct gtaattaaaa cgttgatccc atcgtccggg cagccttttt
     1321 caataactaa cccgtttttt actaattcag ccaaagcaat gcgtacttgc cctacaggtt
     1381 ctttgcggct gataccgaag tcatcttcgt aatccagagt aactatctca cctttgcctg
     1441 cgtaagctgc ctccagaatt ttggacttat ttgcttcaat ctgactgttc tgctgaatat
     1501 ccatcgaacc ctcctacctg gttaattaag cacctatatt accacttttt tgccttaggt
     1561 tttggagggg tcaaggggag gcgaagccgc ccattgcaag gcccgatgtg cgacattgag
     1621 catagcgaaa tgtagcaaca tcgggtagcc ttgtttaaag cgaaatatgc gacgacggag
     1681 gtggaatgga aaagcgcgat aacatgctgc gggtcaggtt ttctgacgcg gaatttgagg
     1741 cgctgaagca gctggccgaa gatgcaggct gtaccatgtc cgagctggtt cgcgatcacc
     1801 tcggcagggt cagcgtgcgc aacaaagacg tcgatcggga gcggattgcc atgcttaacc
     1861 gcatcaatgc caacctgaac atgattgccc gctgggttaa tacgcataaa tcggcggctt
     1921 cctcggttga ggtcgttgct cacctgatgg atatagagcg ccatattcgg gagctgtcgc
     1981 aatgattgtg ggtttcagca agcacggcac tggcggcggc ggtggcccgg tcggctatgt
     2041 caccgataaa acgcgtcccg gtcgggaaaa ttcaccgccg gaagtgctga ggggatcccc
     2101 ggaggctacc cgcaacctta tcgactcgct cgatttccgg cacaaataca cttctggcgt
     2161 gctgtctttc gctccggagg agaaggtcac gccagaaatg gagcaggcgt tcatggatcg
     2221 ctttgaacag gtcgcgttcg ccgggcttga agccgatcag tacaacatcc tctgggtcag
     2281 gcatacccat gccgggcatc acgagctgca ttttgtgacg ccccgcgttg agcttggcac
     2341 cgggaaaagc cttaacatcc gcccgcccgg agatgccgca aaacaggctt tcgatgacct
     2401 gcgcagtgaa atcaacgcca ggtatggcct ggctgatccg gatgacccac ggcgcgcacg
     2461 tgagatcgct cagcctgatc atgagctgaa aatcgccgca gaagctctca gaaggggtga
     2521 gaagccgcag gaagacgtca ggctactcat agacgcggtt ttatctcaga gggccgcaca
     2581 ggggcttata cgcagtcgta gcgatgttat cgagcaggtt tcagaccttg gaatgcaggt
     2641 aacacgcgaa ggtaaaaatt acatcaccgt gtcagatccg cagagcggcc agcggtggcg
     2701 catgaaaggg ggactttatg agcgtgagtt tgacgctgca ggagcgattg aaacagcaac
     2761 atctcgcaga gagcgagact attcaaagcc tgacgaagca gcagctgagc agtttgcgaa
     2821 gcgagttgaa cggcatattt cagcaagagc tgaataccat gctggaagat atgcgcaggc
     2881 agctgcacga cacgacagag gatatgcgcc agagccggca gagcatgttg ctgaccgtcg
     2941 tgaaaaaccg ggtgatattc ccggccctga gcgcggttac gatcttggca gttatctttc
     3001 tgagcggttg gggcctgagt acgtttcagg aaagccagat catggccaac tggcagcaga
     3061 tcaacagcca gaaagccacg ctggcgacgc tggagagcaa aacagcgggt ctgaacatcg
     3121 ttcaggacaa gaacgggaca ttcgtcgtgc tgcccaaagg ggcgaaagtg gacgacagcg
     3181 gctggacagt cgggaagcag caagccctgc ggatagtgag gtaaaccatg acggaactgg
     3241 aaacgcactt attgaacgcc ttgaagcgta tggagcagca attcagcgag cagctgaaag
     3301 cttccgagaa ggcgcaggcc gccttgcaga gcatgttcga gcgtactgcg gaagaca
//
